Progress in studying the impact of hyperlipidemia and statins on rotator cuff injury and repair DOI Creative Commons

Yinhua Qian,

H.C. Huang,

Renwen Wan

et al.

Frontiers in Public Health, Journal Year: 2023, Volume and Issue: 11

Published: Oct. 27, 2023

This review delves into the intersection of two prevalent conditions, hyperlipidemia and rotator cuff injuries, both which bear substantial healthcare burdens. Our investigation begins with an exploration common musculoskeletal disorders that severely impair shoulder functionality quality life. These injuries are notably pervasive among sports enthusiasts older adult, incidence rate estimated at 5–10% in general population. Despite their widespread occurrence diverse, multifactorial etiological factors, effective treatment strategies remain elusive. We then examine hyperlipidemia, a metabolic disorder affecting approximately 40% global adult Characterized by elevated levels cholesterol triglycerides, can precipitate severe cardiovascular complications presents significant socioeconomic burden. Although current management encompass lifestyle modifications pharmacological interventions, condition remains formidable health challenge. Central to this is potential association between injuries. aim synthesize understanding hyperlipidemia’s role pathophysiology thereby offering fresh insights underpinnings, therapeutic targets, drugs, such as Statins. The influence other lipid-lowering therapeutics on tendon also considered, further research molecular pathways benefits these drugs required. pursuit aligns broader efforts enhance patient outcomes, minimize burdens, contribute conditions.

Language: Английский

Low-intensity pulsed ultrasound promotes skeletal muscle regeneration via modulating the inflammatory immune microenvironment DOI Creative Commons
Haocheng Qin, Zhiwen Luo, Yaying Sun

et al.

International Journal of Biological Sciences, Journal Year: 2023, Volume and Issue: 19(4), P. 1123 - 1145

Published: Jan. 1, 2023

Background: Low-intensity pulsed ultrasound (LIPUS, a form of mechanical stimulation) can promote skeletal muscle functional repair, but lack mechanistic understanding its relationship and tissue regeneration limits progress in this field.We investigated the hypothesis that specific energy levels LIPUS mediates by modulating inflammatory microenvironment.Methods: To address these gaps, irritation was applied vivo for 5 min at two different intensities (30mW/cm 2 60mW/cm ) next 7 consecutive days, treatment begun 24h after air drop-induced contusion injury.In vitro experiments, three , 45mW/cm times introduction LPS RAW264.7.Then, we comprehensively assessed histological parameters injury mice phenotype shifting macrophages through molecular biological methods immunofluorescence analysis both vitro.Results: We reported therapy intensity exhibited most significant differences recovery contusion-injured mice.The comprehensive tests indirectly directly proved effectiveness recovery.Through vitro, found improvement attributable part to clearance M1 populations increase M2 subtypes with change macrophage-mediated factors.Depletion eliminated therapeutic effects LIPUS, indicating function result M2-shifted macrophage polarization.Moreover, M2-inducing were partially WNT pathway upregulating FZD5 expression enhancing β-catenin nuclear translocation vivo.The inhibition augment further verified our results.Conclusion: could significantly promoted from M2.The ability direct polarization may be beneficial target clinical many injuries diseases.

Language: Английский

Citations

40
Research progress of exosomes in pathogenesis, diagnosis, and treatment of ocular diseases DOI Creative Commons

Xinting Feng,

Zhen Peng,

Lingyi Yuan

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Jan. 24, 2023

Exosomes are natural extracellular vesicles with a diameter of 30–150 nm, which exist in biological fluids and contain biomolecules related to the parent cell, such as proteins, nucleic acids, lipids, etc. It has wide range functions, participates regulation important physiological pathological activities body. can be used biomarker for early diagnosis ocular diseases, potential therapeutic target, targeted drug carrier, high clinical application. In this paper, we summarized genesis mechanism, research application progress exosomes, focused on engineering strategy advantages disadvantages common exosome preparation methods. Systematically combed role exosomes corneal glaucoma, retinal provide reference further understanding pathogenesis, diagnosis, treatment diseases. Finally, opportunities challenges precision medicine. The extension field ophthalmology will help advance current diagnostic Tiny have huge potential.

Language: Английский

Citations

33
Enhancing diabetic muscle repair through W-GA nanodots: a nanomedicinal approach to ameliorate myopathy in type 2 diabetes DOI Creative Commons
Shan Liu,

Renwen Wan,

Qingrong Li

et al.

Burns & Trauma, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 1, 2025

Abstract Objective Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder that significantly impairs muscle regeneration following injuries, contributing to numerous complications and reduced quality of life. There an urgent need for therapeutic strategies can enhance alleviate these pathological mechanisms. In this study, we evaluate the efficacy W-GA nanodots, which are composed gallic acid (GA) tungstate (W6+), on in type (T2D)-induced injury, with focus their anti-inflammatory antioxidative effects. Methods This study synthesized ultrasmall nanodots were optimized improved stability bioactivity under physiological conditions. vitro assessments included cell viability, apoptosis, reactive oxygen species (ROS) generation, myotube differentiation C2C12 myoblasts hyperglycemic vivo, T2D was induced C57BL/6 mice, followed by injury treatment W-GA. Muscle repair, fibrosis, functional recovery assessed through histological analysis gait using CatWalk system. Results The enhanced proliferation, decreased ROS, apoptosis vitro. compared control group, W-GA-treated group exhibited notably regeneration, recovery. modulated inflammatory response oxidative stress diabetic tissues, facilitating regenerative dynamics function. Conclusions effectively counter mechanisms myopathy enhancing capacity reducing inflammation. nanomedicine approach offers promising avenue improving health overall life individuals suffering from T2D. However, further studies needed explore clinical applications long-term preventing complications.

Language: Английский

Citations

1
Exercise-induced IL-15 acted as a positive prognostic implication and tumor-suppressed role in pan-cancer DOI Creative Commons
Zhiwen Luo, Zhong He, Haocheng Qin

et al.

Frontiers in Pharmacology, Journal Year: 2022, Volume and Issue: 13

Published: Nov. 17, 2022

Objective: Exercise can produce a large number of cytokines that may benefit cancer patients, including Interleukin 15 (IL-15). IL-15 is cytokine has multiple functions in regulating the adaptive and innate immune systems tumorigenesis lung breast cancers. However, roles other types remain unknown. In this article, we try to systematically analyze if potential molecular biomarker for predicting patient prognosis pan-cancer its connection with anti-cancer effects exercise. Methods: The expression was detected by Cancer Genome Atlas (TCGA) database, Human protein (HPA), Genotype Tissue-Expression (GTEX) database. Analysis genomic alterations human organic tissues analyzed cBioPortal database HPA. correlations between survival outcomes, clinical features, immune-associated cell infiltration, ferroptosis/cuproptosis were using TCGA, ESTIMATE algorithm, TIMER databases. Gene Set Enrichment (GSEA) performed evaluate biological pan-cancer. Results: differential analysis suggested level mRNA significantly downregulated 12 tumor compared normal tissues, which similar most types. high could predict positive outcome patients LUAD (lung adenocarcinoma), COAD (colon COADREAD rectum ESCA (esophageal carcinoma), SKCM (skin cutaneous melanoma), UCS (uterine carcinosarcoma), READ (rectum adenocarcinoma). Moreover, amplification found be frequent mutation type genomic. Furthermore, correlated infiltration levels various cells assessed algorithm addition, positively ferroptosis/cuproptosis-related genes (ACSL4 LIPT1) Levels reported elevated humans 10-120 min following an acute Therefore, hypothesized better regular exercise achieved IL-15. Conclusion: Our results demonstrated prognosis, immunoreaction, partly explained

Language: Английский

Citations

29
A Mesoporous Silica‐Loaded Multi‐Functional Hydrogel Enhanced Tendon Healing via Immunomodulatory and Pro‐Regenerative Effects DOI
Renwen Wan, Zhiwen Luo,

Xiaoshuang Nie

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: April 9, 2024

Tendon injuries are pervasive orthopedic encountered by the general population. Nonetheless, recovery after severe injuries, such as Achilles tendon injury, is limited. Consequently, there a pressing need to devise interventions, including biomaterials, that foster healing. Regrettably, tissue engineering treatments have faced obstacles in crafting appropriate scaffolds and efficacious nanomedical approaches. To surmount these hurdles, an innovative injectable hydrogel (CP@SiO2), comprising puerarin chitosan through situ self-assembly, pioneered while concurrently delivering mesoporous silica nanoparticles for In this research, CP@SiO2 employed treatment of conducting extensive vivo vitro experiments evaluate its efficacy. This reults demonstrates enhances proliferation differentiation tendon-derived stem cells, mitigates inflammation modulation macrophage polarization. Furthermore, using histological behavioral analyses, it found can improve biomechanical properties injured tendons. findings indicate multifaceted constitutes suitable bioactive material repair presents promising new strategy clinical management injuries.

Language: Английский

Citations

7
Role of oxidative stress in the concurrent development of osteoporosis and tendinopathy: Emerging challenges and prospects for treatment modalities DOI Creative Commons

Xianting Xia,

Zhengyuan Fang,

Yinhua Qian

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(13)

Published: July 1, 2024

Both osteoporosis and tendinopathy are widely prevalent disorders, encountered in diverse medical contexts. Whilst each condition has distinct pathophysiological characteristics, they share several risk factors underlying causes. Notably, oxidative stress emerges as a crucial intersecting factor, playing pivotal role the onset progression of both diseases. This imbalance arises from dysregulation generating neutralising reactive oxygen species (ROS), leading to an abnormal environment. Elevated levels ROS can induce multiple cellular disruptions, such cytotoxicity, apoptosis activation reduced cell function, contributing tissue deterioration weakening structural integrity bones tendons. Antioxidants substances that prevent or slow down oxidation process, including Vitamin C, melatonin, resveratrol, anthocyanins so on, demonstrating potential treating these overlapping disorders. comprehensive review aims elucidate complex within interlinked pathways comorbid conditions. By integrating contemporary research empirical findings, our objective is outline new conceptual models innovative treatment strategies for effectively managing underscores importance further in-depth validate efficacy antioxidants traditional Chinese medicine plans, well explore targeted interventions focused on promising areas future advancements.

Language: Английский

Citations

7
Different origin-derived exosomes and their clinical advantages in cancer therapy DOI Creative Commons
Xiaoyan Jin, Jing Zhang, Yufu Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: June 27, 2024

Exosomes, as a class of small extracellular vesicles closely related to the biological behavior various types tumors, are currently attracting research attention in cancer diagnosis and treatment. Regarding diagnosis, stability their membrane structure wide distribution body fluids render exosomes promising biomarkers. It is expected that exosome-based liquid biopsy will become an important tool for tumor future. For treatment, exosomes, “golden communicators” between cells, can be designed deliver different drugs, aiming achieve low-toxicity low-immunogenicity targeted delivery. Signaling pathways exosome contents also used safer more effective immunotherapy against tumors. Exosomes derived from range sources, exhibit characteristics well clinical application advantages therapies. In this review, we analyzed main sources have great potential broad prospects therapy. Moreover, compared therapeutic advantages, providing new ideas exosomes.

Language: Английский

Citations

6
Agomir-122-loaded nanoparticles coated with cell membrane of activated fibroblasts to treat frozen shoulder based on homologous targeting DOI Creative Commons
Zhen Peng, Beijie Qi, Zhiwen Luo

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: April 10, 2024

As a common musculoskeletal disorder, frozen shoulder is characterized by thickened joint capsule and limited range of motion, affecting 2-5% the general population more than 20% patients with diabetes mellitus. Pathologically, fibrosis resulting from fibroblast activation key event. The activated fibroblasts are proliferative contractive, producing excessive collagen. Albeit high prevalence, effective anti-fibrosis modalities, especially fibroblast-targeting therapies, still lacking. In this study, microRNA-122 was first identified sequencing data as potential therapeutic agent to antagonize activation. Then, Agomir-122, an analog microRNA-122, loaded into poly(lactic-co-glycolic acid) (PLGA) nanoparticles (Agomir-122@NP), carrier excellent biocompatibility for delivery. Moreover, relying on homologous targeting effect, we coated Agomir-122@NP cell membrane derived (Agomir-122@MNP), attempt inhibit proliferation, contraction, collagen production abnormally fibroblasts. After confirming effect Agomir-122@MNP in vitro, proved that effectively curtailed activation, ameliorated fibrosis, restored motion mouse models both prophylactically therapeutically. Overall, targeted delivery method developed promising translational value against shoulder.

Language: Английский

Citations

5
Endothelial cells-derived exosomes-based hydrogel improved tendinous repair via anti-inflammatory and tissue regeneration-promoting properties DOI Creative Commons

Yichen Dou,

Zhai Hong,

Haiqiu Li

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: July 9, 2024

Tendon injuries are common orthopedic ailments with a challenging healing trajectory, especially in cases like the Achilles tendon afflictions. The trajectory of is often suboptimal, leading to scar formation and functional impairment due inherent low metabolic activity vascularization tissue. As pressing needed for effective interventions, efforts made explore biomaterials augment healing. However, tissue engineering approaches face hurdles optimizing scaffolds nanomedical strategies. To navigate these challenges, an injectable hydrogel amalgamated human umbilical vein endothelial cells-derived exosomes (HUVECs-Exos) was prepared named H-Exos-gel this study, aiming enhance repair. In our research involving model 60 rats, we investigated efficacy through histological assessments performed at 2 4 weeks behavioral conducted 4-week mark revealed its ability tendon's mechanical strength, regulate inflammation facilitate regeneration recovery. Mechanically, modulated cellular behaviors macrophages tendon-derived stem cells (TDSCs) by inhibiting inflammation-related pathways promoting proliferation-related pathways. Our findings delineate that epitomizes viable bioactive medium healing, heralding promising avenue clinical amelioration injuries.

Language: Английский

Citations

5
Macrophage-Myofibroblast Transition as a Potential Origin for Skeletal Muscle Fibrosis After Injury via Complement System Activation DOI Creative Commons
Beijie Qi, Yuqi Li, Zhen Peng

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 1083 - 1094

Published: Feb. 1, 2024

Acute skeletal muscle injury is common in sports. The injured cannot fully recover due to fibrosis resulting from myofibroblasts. Understanding the origin of fibroblasts is, therefore, important for development anti-fibrotic therapies. Accumulating evidence shows that a mechanism called macrophage-myofibroblast transition (MMT) can lead tissue or organ fibrosis, yet it still unclear whether MMT exists and exact mechanisms.

Language: Английский

Citations

4