Interfacing with the Brain: How Nanotechnology Can Contribute DOI Creative Commons
Abdullah Ahmed Ali Ahmed, Núria Alegret, Bethany Almeida

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Interfacing artificial devices with the human brain is central goal of neurotechnology. Yet, our imaginations are often limited by currently available paradigms and technologies. Suggestions for brain-machine interfaces have changed over time, along technology. Mechanical levers cable winches were used to move parts during mechanical age. Sophisticated electronic wiring remote control arisen age, ultimately leading plug-and-play computer interfaces. Nonetheless, brains so complex that these visions, until recently, largely remained unreachable dreams. The general problem, thus far, most technology mechanically and/or electrically engineered, whereas a living, dynamic entity. As result, worlds difficult interface one another. Nanotechnology, which encompasses engineered solid-state objects integrated circuits, excels at small length scales single few hundred nanometers and, thus, matches sizes biomolecules, biomolecular assemblies, cells. Consequently, we envision nanomaterials nanotools as opportunities in alternative ways. Here, review existing literature on use nanotechnology look forward discussing perspectives limitations based authors' expertise across range complementary disciplines─from neuroscience, engineering, physics, chemistry biology medicine, science mathematics, social jurisprudence. We focus but also include information from related fields when useful complementary.

Language: Английский

Vascularization of Human Brain Organoids DOI Open Access
Takeshi K. Matsui,

Yuichiro Tsuru,

Koichi Hasegawa

et al.

Stem Cells, Journal Year: 2021, Volume and Issue: 39(8), P. 1017 - 1024

Published: March 31, 2021

Human brain organoids are three-dimensional tissues that generated in vitro from pluripotent stem cells and recapitulate the early development of human brain. Brain consist mainly neural lineage cells, such as stem/precursor neurons, astrocytes, oligodendrocytes. However, all lack vasculature, which plays indispensable roles not only homeostasis but also development. In addition to delivery oxygen nutrition, accumulating evidence suggests vascular system regulates differentiation, migration, circuit formation during Therefore, vascularization is great importance. Current trials vascularize various include adjustment cultivation protocols, introduction microfluidic devices, transplantation into immunodeficient mice. this review, we summarize efforts accomplish perfusion organoids, discuss these attempts a forward-looking perspective.

Language: Английский

Citations

126
Generation of hypothalamic arcuate organoids from human induced pluripotent stem cells DOI Creative Commons

Wei‐Kai Huang,

Samuel Zheng Hao Wong,

Sarshan R. Pather

et al.

Cell stem cell, Journal Year: 2021, Volume and Issue: 28(9), P. 1657 - 1670.e10

Published: May 6, 2021

Language: Английский

Citations

125
An in vivo neuroimmune organoid model to study human microglia phenotypes DOI Open Access
Simon T. Schafer, Abed AlFatah Mansour, Johannes C. M. Schlachetzki

et al.

Cell, Journal Year: 2023, Volume and Issue: 186(10), P. 2111 - 2126.e20

Published: May 1, 2023

Language: Английский

Citations

124
Bioengineering Approaches for the Advanced Organoid Research DOI
Sang Ah Yi, Yixiao Zhang, Christopher Rathnam

et al.

Advanced Materials, Journal Year: 2021, Volume and Issue: 33(45)

Published: Sept. 24, 2021

Abstract Recent advances in 3D cell culture technology have enabled scientists to generate stem derived organoids that recapitulate the structural and functional characteristics of native organs. Current organoid technologies been striding toward identifying essential factors for controlling processes involved development, including physical cues biochemical signaling. There is a growing demand engineering dynamic niches characterized by conditions resemble vivo organogenesis reproducible reliable various applications. Innovative biomaterial‐based advanced engineering‐based approaches incorporated into conventional methods facilitate development research. The recent engineering, extracellular matrices genetic modulation, are comprehensively summarized pinpoint parameters critical organ‐specific patterning. Moreover, perspective trends developing tunable response exogenous endogenous discussed next‐generation developmental studies, disease modeling, therapeutics.

Language: Английский

Citations

116
iPS-cell-derived microglia promote brain organoid maturation via cholesterol transfer DOI

Dong Shin Park,

Tatsuya Kozaki, Satish Kumar Tiwari

et al.

Nature, Journal Year: 2023, Volume and Issue: 623(7986), P. 397 - 405

Published: Nov. 1, 2023

Language: Английский

Citations

104
Structural and functional integration of human forebrain organoids with the injured adult rat visual system DOI Creative Commons
Dennis Jgamadze,

James Lim,

Zhijian Zhang

et al.

Cell stem cell, Journal Year: 2023, Volume and Issue: 30(2), P. 137 - 152.e7

Published: Feb. 1, 2023

Language: Английский

Citations

87
Human fetal brain self-organizes into long-term expanding organoids DOI Creative Commons
Delilah Hendriks, Anna Pagliaro, Francesco Andreatta

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(3), P. 712 - 732.e38

Published: Jan. 8, 2024

Human brain development involves an orchestrated, massive neural progenitor expansion while a multi-cellular tissue architecture is established. Continuously expanding organoids can be grown directly from multiple somatic tissues, yet to date, solely established pluripotent stem cells. Here, we show that healthy human fetal in vitro self-organizes into (FeBOs), phenocopying aspects of vivo cellular heterogeneity and complex organization. FeBOs expanded over long time periods. FeBO growth requires maintenance integrity, which ensures production tissue-like extracellular matrix (ECM) niche, ultimately endowing expansion. lines derived different areas the central nervous system (CNS), including dorsal ventral forebrain, preserve their regional identity allow probe positional identity. Using CRISPR-Cas9, showcase generation syngeneic mutant for study cancer. Taken together, constitute complementary CNS organoid platform.

Language: Английский

Citations

46
Morphological diversification and functional maturation of human astrocytes in glia-enriched cortical organoid transplanted in mouse brain DOI
Meiyan Wang, Lei Zhang, Sammy Weiser Novak

et al.

Nature Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 28, 2024

Language: Английский

Citations

34
Single-cell mapping of lipid metabolites using an infrared probe in human-derived model systems DOI Creative Commons
Yeran Bai,

Carolina M. Camargo,

Stella M.K. Glasauer

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 8, 2024

Abstract Understanding metabolic heterogeneity is the key to uncovering underlying mechanisms of metabolic-related diseases. Current imaging studies suffer from limitations including low resolution and specificity, model systems utilized often lack human relevance. Here, we present a single-cell platform enable direct lipid metabolism with high specificity in various human-derived 2D 3D culture systems. Through incorporation an azide-tagged infrared probe, selective detection newly synthesized lipids cells tissue became possible, while simultaneous fluorescence enabled cell-type identification complex tissues. In proof-of-concept experiments, were directly visualized human-relevant among different cell types, mutation status, differentiation stages, over time. We identified upregulated progranulin-knockdown induced pluripotent stem their differentiated microglia cells. Furthermore, observed that neurons brain organoids exhibited significantly lower compared astrocytes.

Language: Английский

Citations

28
An integrated transcriptomic cell atlas of human neural organoids DOI Creative Commons
Zhisong He, Leander Dony, Jonas Simon Fleck

et al.

Nature, Journal Year: 2024, Volume and Issue: 635(8039), P. 690 - 698

Published: Nov. 20, 2024

Human neural organoids, generated from pluripotent stem cells in vitro, are useful tools to study human brain development, evolution and disease. However, it is unclear which parts of the covered by existing protocols, has been difficult quantitatively assess organoid variation fidelity. Here we integrate 36 single-cell transcriptomic datasets spanning 26 protocols into one integrated cell atlas totalling more than 1.7 million cells1–26. Mapping developing references27–30 shows primary types states that have estimates similarity between counterparts across protocols. We provide a programmatic interface browse query new datasets, showcase power annotate evaluate Finally, show can be used as diverse control cohort compare models disease, identifying genes pathways may underlie pathological mechanisms with models. The will fidelity, characterize perturbed diseased facilitate protocol development. A integrating counterparts, showing potential fidelity

Language: Английский

Citations

27