Drosophila melanogaster as a model for nutrient regulation of ovarian function

Reproduction, Journal Year: 2019, Volume and Issue: 159(2), P. R69 - R82

Published: Sept. 5, 2019

Observed in a wide variety of organism, from invertebrates to mammals, nutritional status modulates the energetically costly effort producing female gametes. Despite this long-standing link between nutrition and ovarian function, relatively little is known about cellular molecular mechanisms that underlie how dietary components modulate egg production. Drosophila melanogaster , with its powerful extensive genetic tools as well well-characterized response diet, has proven be instrumental addressing issue. This review covers what we currently know control oogenesis salient features fruit fly make it model for function.

Language: Английский

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Effects of dietary protein:carbohydrate balance on life‐history traits in six laboratory strains of Drosophila melanogaster

Entomologia Experimentalis et Applicata, Journal Year: 2019, Volume and Issue: 168(6-7), P. 482 - 491

Published: Nov. 26, 2019

Abstract Drosophila melanogaster Meigen (Diptera: Drosophilidae) is a key model insect for studying life span and aging. Many laboratory strains of D. are currently used by laboratories worldwide, but they known to vary considerably in their physiology, behavior, histories. Although the importance dietary protein:carbohydrate (P:C) balance as predominant determinant other life‐history traits has been highlighted recent research, it remains unexplored whether impacts P:C on these fitness‐related strain‐specific manner . In this study, we compared consequences (life span, egg production rate, pre‐adult survival, development time, body mass at eclosion) six ( w1118 , yw Oregon‐R white Canton‐S Canton‐S‐SNU Canton‐S‐Inha ) allocated one four synthetic diets differing ratio (1:16, 1:4, 1:1, or 4:1). The effects various adult larval were qualitatively similar across all studied study. Regardless fly strain, adults exhibited shortened improved diet with highest 4:1. strains, larvae raised comprising lowest 1:16 suffered high mortality, delayed reduced mass. Despite general similarity direction effect differed magnitude responses balance, indicated significant interaction between strain measured except eclosion. Possible mechanisms explaining such discussed.

Language: Английский

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Genetic independence between traits separated by metamorphosis is widespread but varies with biological function

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2023, Volume and Issue: 290(2010)

Published: Nov. 8, 2023

Why is metamorphosis so pervasive? Does it facilitate the independent (micro)evolution of quantitative traits in distinct life stages, similarly to how enables some limbs and organs develop at specific stages? We tested this hypothesis by measuring expression 6400 genes 41 Drosophila melanogaster inbred lines larval adult stages. Only 30% showed significant genetic correlations between expression. By contrast, 46% level independence Gene ontology terms enrichment revealed that across stages correlated were often involved proteins synthesis, insecticide resistance innate immunity, while a vast number associated with energy metabolism compared our results similar case: constraints males females gonochoric species (i.e. sexual antagonism). expected selection for separation be higher than juvenile functions, as gonochorism more common strategy animal kingdom metamorphosis. Surprisingly, we found inter-stage lower inter-sexual constraints. Overall, show large part transcriptome evolve independently different

Language: Английский

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High-Sucrose Diet Exposure on Larvae Contributes to Adult Fecundity and Insecticide Tolerance in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel)

Insects, Journal Year: 2023, Volume and Issue: 14(5), P. 407 - 407

Published: April 24, 2023

Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) is one of the broad host ranges and economically-important insect pests in tropical subtropical areas. A wide range hosts means they have strong adaptation ability to changes dietary macronutrients (e.g., sucrose protein). However, effects conditions on phenotypes genotypes B. are still unclear. In this study, we aimed investigate larval life history traits stress tolerance dorsalis, its defense response at molecular level. The results showed that low-sucrose (LS) induced decreased body size, shortened developmental duration, enhanced sensitivity beta-cypermethrin. Otherwise, high-sucrose (HS) diet increased adult fecundity, malathion. Based transcriptome data, 258 904 differentially expressed genes (DEGs) were identified NS (control) versus LS groups, HS respectively. These yielded DEGs relevant multiple specific metabolisms, hormone synthesis signaling, immune-related pathways. Our study will provide biological perspective understand phenotypic adjustments diets adaptability oriental fruit flies.

Language: Английский

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An overview of two decades of diet restriction studies using Drosophila

Biogerontology, Journal Year: 2019, Volume and Issue: 20(6), P. 723 - 740

Published: Aug. 2, 2019

Language: Английский

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Thermal and nutritional environments during development exert different effects on adult reproductive success in Drosophila melanogaster

Ecology and Evolution, Journal Year: 2020, Volume and Issue: 11(1), P. 443 - 457

Published: Nov. 24, 2020

Abstract Environments experienced during development have long‐lasting consequences for adult performance and fitness. The “environmental matching” hypothesis predicts that individuals perform best when developmental environments match whereas the “silver spoon” expects fitness is higher in developed under favorable regardless of environments. Temperature nutrition are two most influential determinants environmental quality, but it remains to be elucidated which these hypotheses better explains long‐term effects thermal nutritional histories on traits. Here we compared how temperature larval environment would affect survivorship reproductive success fruit fly, Drosophila melanogaster . aspect focused this study was dietary protein‐to‐carbohydrate (P:C) ratio. impact low improve survivorship. High P:C diet had a negative effect ingested stage, positive development. No matter whether matched or not, females raised warm protein‐enriched produced more eggs than those cool protein‐limiting environments, suggesting presence significant silver spoon nutrition. egg production weak persisted across early stage initially strong diminished rapidly after day 5 posteclosion. Egg strongly influenced by ratio diet, indicating contributing mainly reproduction shifted from diet. Our results highlight importance shaping organismal also demonstrate aspects differ fundamentally their nature, strength, persistence.

Language: Английский

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Physiological demands and nutrient intake modulate a trade-off between dispersal and reproduction based on age and sex of field crickets

Journal of Experimental Biology, Journal Year: 2021, Volume and Issue: 224(7)

Published: April 1, 2021

ABSTRACT Animals adjust resource acquisition throughout life to meet changing physiological demands of growth, reproduction, activity and somatic maintenance. Wing-polymorphic crickets invest in either dispersal or reproduction during early adulthood, providing a system which determine how variation demands, determined by sex history strategy, impact nutritional targets, plus the consequences nutritionally imbalanced diets across stages. We hypothesized that high biosynthesis (especially oogenesis females) drive elevated requirements confer vulnerability diets. Nutrient targets allocation into key tissues associated with investments were for juvenile adult male female field (Gryllus lineaticeps) when given choice between two calorically equivalent but (protein- carbohydrate-biased) artificial diets, restricted one diet. Flight muscle synthesis drove general caloric juveniles investing dispersal, flight quality was robust Testes not costly, males insensitive diet composition. In contrast, costs ovarian protein females. When constrained carbohydrate-biased diet, ovary reduced reproductive morph females, eliminating their advantage fecundity over morph. Our findings demonstrate nutrient modulates dispersal–reproduction trade-offs an age- sex-specific manner. Declines food will thus disproportionately affect specific cohorts, potentially driving demographic shifts altering patterns evolution.

Language: Английский

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Fat Quality Impacts the Effect of a High-Fat Diet on the Fatty Acid Profile, Life History Traits and Gene Expression in Drosophila melanogaster

Cells, Journal Year: 2022, Volume and Issue: 11(24), P. 4043 - 4043

Published: Dec. 14, 2022

Feeding a high-fat diet (HFD) has been shown to alter phenotypic and metabolic parameters in Drosophila melanogaster. However, the impact of fat quantity quality remains uncertain. We first used butterfat (BF) as an example investigate effects increasing dietary content (3–12%) on male female fruit flies. Although body weight composition were not altered by any BF concentration, health parameters, such lifespan, fecundity larval development, negatively affected dose-dependent manner. When flies fed various 12% HFDs (BF, sunflower oil, olive linseed fish oil), their fatty acid profiles shifted according qualities. Moreover, was found determine effect size response HFD for traits, climbing activity, or fertility. Consistently, we also highly quality-specific transcriptional three exemplary qualities with small overlap only 30 differentially expressed genes associated immune/stress metabolism. In conclusion, our data indicate that but is crucial factor terms life-history traits when applying D.

Language: Английский

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Biosynthetic constraints on amino acid synthesis at the base of the food chain may determine their use in higher-order consumer genomes

PLoS Genetics, Journal Year: 2023, Volume and Issue: 19(2), P. e1010635 - e1010635

Published: Feb. 13, 2023

Dietary nutrient composition is essential for shaping important fitness traits and behaviours. Many organisms are protein limited, Drosophila melanogaster this limitation manifests at the level of single most limiting Amino Acid (AA) in diet. The identity AA its effects on female fecundity readily predictable by a procedure called exome matching which sum AAs encoded consumer’s used to predict relative proportion required However, calculation does not weight contributions overall profile size or expression. Here, we update include these weightings. Surprisingly, although nearly half transcriptome differentially expressed when comparing male flies, found that creating transcriptome-weighted matched diets each sex did enhance their over supported alone. These data indicate while may require different amounts dietary across conditions, constituent remains constant. Interestingly, also profiles generally conserved taxa might be explained energetic elemental limitations microbial synthesis. Thus, it appears ecological constraints amongst autotrophs shape available trophic levels constrains biomass composition.

Language: Английский

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Towards an integrated understanding of dietary phenotypes

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2023, Volume and Issue: 378(1891)

Published: Oct. 15, 2023

Diet and nutrition comprise a complex, multi-faceted interface between animal biology food environments. With accumulating information on the many facets of this association arises need for systems-based approaches that integrate dietary components their links with ecology, feeding, post-ingestive processes functional ecological consequences these interactions. We briefly show how modelling approach, nutritional geometry, has used experimental control afforded in laboratory studies to begin unravel links. Laboratory studies, however, have limited ability establish whether feeding physiological mechanisms realistic next provide an overview observational field free-ranging primates examined this, producing largely correlative data suggesting similar operate wild as laboratory. Significant challenges remain, establishing causal resource variation wild. end more detailed account two temperate capitalized discrete provided by seasonal environments strengthen inference link patterns intake dynamics nutrient processing. This article is part theme issue 'Food processing assimilation animals'.

Language: Английский

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