Extracellular vesicles: roles in gamete maturation, fertilization and embryo implantation

Human Reproduction Update, Journal Year: 2015, Volume and Issue: unknown, P. dmv055 - dmv055

Published: Dec. 9, 2015

BACKGROUNDExtracellular vesicles (EVs) are membrane-bound vesicles, found in biofluids, that carry and transfer regulatory molecules, such as microRNAs (miRNAs) proteins, may mediate intercellular communication between cells tissues. EVs have been isolated from a wide variety of including plasma, urine, and, relevant to this review, seminal, follicular uterine luminal fluid. We conducted systematic search the literature review present currently available evidence on possible roles growth, resumption oocyte development maturation (meiosis), sperm maturation, fertilization embryo implantation.

Language: Английский

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The emerging role of extracellular vesicles as biomarkers for urogenital cancers

Nature Reviews Urology, Journal Year: 2014, Volume and Issue: 11(12), P. 688 - 701

Published: Nov. 18, 2014

Language: Английский

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Human Endometrial Exosomes Contain Hormone-Specific Cargo Modulating Trophoblast Adhesive Capacity: Insights into Endometrial-Embryo Interactions1

Biology of Reproduction, Journal Year: 2016, Volume and Issue: 94(2)

Published: Jan. 14, 2016

Embryo implantation into receptive endometrium requires synergistic endometrial-blastocyst interactions within the uterine cavity and is essential for establishing pregnancy. We demonstrate that exosomes (40–150 nm nanovesicles) released from endometrial epithelial cells are an important component of these interactions. defined proteome purified epithelial-derived (Exos) influenced by menstrual cycle hormones estrogen (E; proliferative phase) plus progesterone (EP; examined their potential to modify trophoblast function. E-/EP-Exos were uniquely enriched with 254 126 proteins, respectively, 35% newly identified proteins not previously reported in exosome databases. Importantly, EP-Exos protein cargo was related fundamental changes implantation: adhesion, migration, invasion, extracellular matrix remodeling. These findings hormonally treated ECC1 cancer validated human primary cell-derived exosomes. Functionally, internalized enhanced adhesive capacity, a response mediated partially through active focal adhesion kinase (FAK) signaling. Thus, contribute endometrial-embryo microenvironment successful implantation.

Language: Английский

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Meta-signature of human endometrial receptivity: a meta-analysis and validation study of transcriptomic biomarkers

Scientific Reports, Journal Year: 2017, Volume and Issue: 7(1)

Published: Aug. 24, 2017

Abstract Previous transcriptome studies of the human endometrium have revealed hundreds simultaneously up- and down-regulated genes that are involved in endometrial receptivity. However, overlap between is relatively small, we still searching for potential diagnostic biomarkers. Here perform a meta-analysis endometrial-receptivity associated on 164 samples (76 from ‘pre-receptive’ 88 mid-secretory, ‘receptive’ phase endometria) using robust rank aggregation (RRA) method, followed by enrichment analysis, regulatory microRNA prediction. We identify meta-signature receptivity involving 57 mRNA as putative markers, where 39 these confirm experimentally RNA-sequencing method two separate datasets. The highlight importance immune responses, complement cascade pathway involvement exosomes mid-secretory functions. Bioinformatic prediction identifies 348 microRNAs could regulate 30 genes, decreased expression 19 with 11 corresponding up-regulated our validation experiments. identified pathways, together their serve promising sought-after biomarkers receptivity, fertility infertility.

Language: Английский

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New insights into extracellular vesicle biogenesis and function

Journal of Cell Science, Journal Year: 2019, Volume and Issue: 132(13)

Published: July 1, 2019

ABSTRACT It is becoming increasingly evident that most cell types are capable of forming and releasing multiple distinct classes membrane-enclosed packages, referred to as extracellular vesicles (EVs), a form intercellular communication. Microvesicles (MVs) represent one the major EVs formed by outward budding plasma membrane. The second class EVs, exosomes, produced components multivesicular bodies (MVBs) released from cells when MVBs fuse with surface. Both MVs exosomes have been shown contain proteins, RNA transcripts, microRNAs even DNA can be transferred other thereby trigger broad range cellular activities biological responses. However, EV biogenesis also frequently de-regulated in different pathologies, especially cancer, where suggested promote tumor growth, therapy resistance, invasion metastasis. In this Review, we highlight some recent advances rapidly emerging exciting field biology, focusing on underlying mechanisms drive MV exosome formation release, particular emphasis how potentially impact aspects cancer progression stem biology.

Language: Английский

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Oviduct extracellular vesicles protein content and their role during oviduct–embryo cross-talk

Reproduction, Journal Year: 2017, Volume and Issue: 154(3), P. 253 - 268

Published: June 20, 2017

Successful pregnancy requires an appropriate communication between the mother and embryo. Recently, exosomes microvesicles, both membrane-bound extracellular vesicles (EVs) present in oviduct fluid have been proposed as key modulators of this unique cross-talk. However, little is known about their content role during oviduct-embryo dialog. Given differences secretions by vivo vitro epithelial cells (OEC), we aimed at deciphering EVs protein from sources. Moreover, analyzed functional effect on embryo development. Our study demonstrated for first time substantial secretion/content. Mass spectrometry analysis identified 319 proteins EVs, which 186 were differentially expressed when compared (P < 0.01). Interestingly, 97 exclusively 47 only 175 common. Functional revealed involved sperm-oocyte binding, fertilization development, some them lacking EVs. showed that vitro-produced embryos able to internalize culture with a In increased blastocyst rate, extended survival over improved quality. provides characterization increasing our understanding gamete/embryo-oviduct interactions. results point promising tools improve development under conditions.

Language: Английский

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Extracellular Vesicles in Human Reproduction in Health and Disease

Endocrine Reviews, Journal Year: 2018, Volume and Issue: 39(3), P. 292 - 332

Published: Jan. 30, 2018

Extensive evidence suggests that the release of membrane-enclosed compartments, more commonly known as extracellular vesicles (EVs), is a potent newly identified mechanism cell-to-cell communication both in normal physiology and pathological conditions. This review presents about formation different EVs, their definitive markers cargo content reproductive physiological processes, capacity to convey information between cells through transfer functional protein genetic alter phenotype function recipient associated with biology. In male tract, epididymosomes prostasomes participate regulating sperm motility activation, capacitation, acrosome reaction. female follicular fluid, oviduct/tube, uterine cavity EVs are considered vehicles carry during oocyte maturation, fertilization, embryo-maternal crosstalk. via might be also involved triggering, maintenance, progression reproductive- obstetric-related pathologies such endometriosis, polycystic ovarian syndrome, preeclampsia, gestational diabetes, erectile dysfunction. this review, we provide current knowledge on present future use not only biomarkers, but therapeutic targeting agents, mainly vectors for drug or compound delivery into target tissues.

Language: Английский

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Extracellular Vesicles from BOEC in In Vitro Embryo Development and Quality

PLoS ONE, Journal Year: 2016, Volume and Issue: 11(2), P. e0148083 - e0148083

Published: Feb. 4, 2016

To evaluate the effect of conditioned media (CM) and Extracellular Vesicles (EVs) derived from bovine oviduct epithelial cell (BOEC) lines on developmental capacity zygotes quality embryos produced in vitro, presumptive were cultured under specific conditions. In experiment 1, either monolayers BOEC extended culture (E), together with fresh suspension cells, or BOEC-CM E-monolayers. 2, EVs isolated characterized (150–200 nm) by Nanosight® electron microscopy. Zygotes presence 3x105EVs/mL, 1.5x105EVs/mL 7.5x104EVs/mL frozen BOEC-EVs. 3, absence FCS but BOEC-E that had been different media. 4, SOF+5% normal-FCS, EV-depleted-FCS. all cases, cleavage rate (Day 2) blastocyst development 7–9) was assessed. Blastocysts Days 7/8 used for evaluation through differential count, cryotolerance gene expression patterns. No differences found among FCS-containing groups yield. However, more trophectoderm while BOEC-EVs, both frozen, has total cells. More survived vitrification BOEC-EV groups. contrast, EV-depleted-FCS than normal-FCS group. Gene patterns modified PAG1 IFN-T, PLAC8, PAG1, CX43, GAPDH FCS. conclusion, have a deleterious embryo quality. during vitro positive embryos, suggesting functional communication between early stages development.

Language: Английский

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Extracellular Vesicles Originate from the Conceptus and Uterus During Early Pregnancy in Sheep1

Biology of Reproduction, Journal Year: 2016, Volume and Issue: 94(3)

Published: Jan. 28, 2016

Cells release diverse types of membrane-bound vesicles endosomal and plasma membrane origin, termed exosomes microvesicles, respectively. Extracellular (EVs) represent an important mode intercellular communication by transferring select RNAs, proteins, lipids between cells. The present studies tested the hypothesis that elongating ovine conceptus uterus produces EVs mediate conceptus-maternal interactions during early pregnancy. In Study 1, were purified from uterine luminal fluid Day 14 cyclic sheep. fluorescently labeled with PKH67 dye infused into lumen pregnant sheep for 6 days using osmotic pump. On 14, observed in trophectoderm epithelia, but not stroma or myometrium. 2, conceptuses cultured ex vivo 24 h found to culture medium. Proteomics analysis conceptus-derived identified 231 proteins enriched extracellular space several protein classes, including proteases, protease inhibitors, chaperones chaperonins. RNA sequencing detected expression 512 mRNAs. top-expressed genes overrepresented ribosomal functions components. Isolated Labeled ovary other maternal tissues. These support ideas emanate both are involved those cells establishment pregnancy

Language: Английский

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Tetraspanins, More than Markers of Extracellular Vesicles in Reproduction

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(20), P. 7568 - 7568

Published: Oct. 14, 2020

The participation of extracellular vesicles in many cellular processes, including reproduction, is unquestionable. Although currently, the tetraspanin proteins found are mostly applied as markers, increasing evidence points to their role vesicle biogenesis, cargo selection, cell targeting, and uptake under both physiological pathological conditions. In this review, we bring other insight into involvement physiology mammalian reproduction. We provide knowledge regarding tetraspanins these processes somatic cells. Furthermore, discuss future direction towards an understanding functions tissues fluids reproductive system gamete maturation, fertilization, embryo development; mutual contact communication complexity.

Language: Английский

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