RNAi in fruit flies (Diptera: Tephritidae): Successes and challenges

Journal of Applied Entomology, Journal Year: 2021, Volume and Issue: 145(8), P. 740 - 756

Published: June 12, 2021

Abstract Fruit flies (Diptera: Tephritidae) are among the most economically important pests worldwide, causing direct damage to fruit crops through female oviposition and larval feeding. Currently, widely disseminated methods of control involve adoption cultural biological techniques, like sterile insect technique, use agrochemicals. However, latter depend on concomitant other especially chemical ones, which successful, but also related resistance, off‐target effects pollution, showing need for new methods. RNA interference (RNAi) is a biotechnological tool that capable inducing post‐transcriptional gene silencing using double‐stranded (dsRNA). The mechanism could be exploited in pest associated with integrated management, by targeting essential genes or increasing susceptibility insects insecticides. Here, we discuss RNAi machinery uptake main Tephritidae species their differences comparison insects. Then, investigate international literature application this improve enhance insects’ insecticides modulate growth development, demonstrating sensibility RNAi. Finally, challenges future as method Tephritidae, such delivery procedures, degradation production dsRNA, effects, occurrence refractoriness, perspectives shortages its considered.

Language: Английский

---

Sublethal impacts of pyriproxyfen on biological traits of non-target species, Drosophila melanogaster (Diptera: Drosophilidae)

Bioagro, Journal Year: 2025, Volume and Issue: 37(1), P. 13 - 24

Published: Jan. 1, 2025

Pyriproxyfen, a juvenile hormone analog (JHA), is considered as reduced-risk alternative to synthetic pesticides for crop protection. It has been frequently used in agriculture and public health manage insect pests. However, recent studies have reported that pyriproxyfen may adverse physiological effects on non-target organisms. This study investigated the of sublethal doses endocrine disrupting insecticide Drosophila melanogaster Meigen (Diptera: Drosophilidae) biological model. Results showed had noticeable effect developmental stages individuals exposed generation. Pyriproxyfen treatment significantly shortens adult longevity both sexes, female male. Finally, these results suggest reproduction capacity D. impacted by reducing number progeny after parent’s generation with pyriproxyfen. These research findings indicate exposure induces affects offspring growth rates insects Drosophila.

Language: Английский

---

Functional Characterization of Vitellogenin Unveils Novel Roles in RHBP Uptake and Lifespan Regulation in the Insect Vector Rhodnius prolixus

Insect Biochemistry and Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 104301 - 104301

Published: March 1, 2025

Language: Английский

---

Bioinformatics-Based Management of Vitellogenin-like Protein’s Role in Pathogen Defense in Nicotiana tabacum L.

Applied Sciences, Journal Year: 2025, Volume and Issue: 15(8), P. 4463 - 4463

Published: April 18, 2025

The primary objective of this study was to identify and characterize pathogen defense proteins in the Nicotiana tabacum L. proteome, focusing on their structural, functional, evolutionary properties, as well interactions with pathogen-derived molecules. Specifically, we aimed comprehensively analyze proteome pinpoint potential uncharacterized defense-related protein that has emerging roles immune responses antioxidant activity across plants animals. Through integrated computational approaches, determined relationships, structural modeling selected performed using different software, followed by validation through multiple metrics, including stereochemical checks (Ramachandran plot), MolProbity analysis, Z-scores. We further investigated functional binding regions or interaction sites. molecular docking investigate between pathogen-associated patterns (PAMPs), specifically β-glucan peptidoglycan (PGN), elucidate defensive mechanisms. Last, normal mode analysis (NMA), dynamics simulation (MDS), post-simulation analyses were employed evaluate stability mobility protein–ligand complexes. Uncharacterized vitellogenin-like (VLP: ID A0A1S4CXB2) domain chosen because its predicted immune-related features, stress response patterns, unknown role at new immunity functions. Phylogenetic revealed significant sequence homology VLPs from other members Solanaceae family. Structural showed a high-quality model, studies indicating stronger affinity for PGN (−10.16 kcal/mol) (−7.19 kcal/mol), highlighting involvement defense. NMA, MDS, exhibits more substantial extensive VLP than β-glucan. Our findings confirmed N. may function pattern recognition receptors (PRRs), capable recognizing responding pathogens activating signaling pathways. Future experimental these could plant application biotechnological approaches sustainable agriculture.

Language: Английский

---

Identification and characterization of doublesex in Bemisia tabaci

Insect Molecular Biology, Journal Year: 2018, Volume and Issue: 27(5), P. 620 - 632

Published: April 16, 2018

Abstract Bemisia tabaci (Gennadius) is an important agricultural pest with a worldwide distribution. Although B. known to have unique haplodiploid reproductive strategy, its sex determination mechanism largely unknown. In this study, we cloned the full‐length sequence of doublesex ( Btdsx ) and found that has 28 splicing isoforms. We two new isoforms transformer 2 Bttra2 ), which encode proteins. also confirmed both genes lack sex‐specific Real‐time quantitative PCR analysis showed expression higher in males than females. RNA interference affected vice versa. Furthermore, silencing or caused malformation male genitalia (anal style). It did not affect female phenotype, but reduced vitellogenin gene These results indicate associated each other are for formation. addition increasing our understanding roles dsx tra2 , will be useful studies species.

Language: Английский

---

Molecular Characterization of Vitellogenin and Its Receptor in Sogatella furcifera, and Their Function in Oocyte Maturation

Frontiers in Physiology, Journal Year: 2019, Volume and Issue: 10

Published: Dec. 18, 2019

The yolk protein precursor, vitellogenin (Vg), provides nutrition for embryonic development whereas the receptor (VgR) is responsible uptake of by maturing oocytes. These two proteins are key reproduction-related in insects. We cloned and characterized Vg VgR genes Sogatella furcifera, investigated their function oocyte maturation. Cloned SfVg SfVgR have open reading frames 6,114 5,796 bp, encoding 2,037 1,931 amino acid residues, respectively. Structural analysis indicates that has three conserved LPD_N, DUF1943, VWFD domains, contains all conservative motifs LDLR superfamily. Both were highly expressed adult females; was most fat body mainly ovary. Knockdown either gene reduced deposition oocytes arrested However, silencing one these did not affect transcript level other. results demonstrate role transporting into essential maturation S. furcifera both could potentially be targeted as means controlling this pest.

Language: Английский

---

Effects of Sublethal Concentrations of Insecticides on the Fecundity of Sogatella furcifera (Hemiptera: Delphacidae) via the Regulation of Vitellogenin and Its Receptor

Journal of Insect Science, Journal Year: 2020, Volume and Issue: 20(5)

Published: Aug. 25, 2020

Abstract White-backed planthopper (Sogatella furcifera, Hemiptera: Delphacidae) is an important migratory pest of rice. It causes severe economic losses by reducing crop production. Vg and VgR are proteins that help in the successful reproduction insects have been studied many insects. To understand molecular mechanisms underlying effects insecticides on white-backed reproduction, we expression profiles SfVg, SfVg-like, SfVgR exposed to insecticides. SfVg silencing inhibited ovarian development, number eggs laid by, hatching rate planthopper. Thiamethoxam LC10 significantly SfVg-like expression. In contrast, triazophos LC25 promoted increased vitellogenin content These results demonstrate can regulate altering SfVgR, thereby affecting population density findings build a foundation for improving our understanding resurgence pests.

Language: Английский

---

New insight into molecular mechanisms underlying division of labor in honeybees

Current Opinion in Insect Science, Journal Year: 2023, Volume and Issue: 59, P. 101080 - 101080

Published: June 28, 2023

Language: Английский

---

The use of gene expression to unravel the single and mixture toxicity of abamectin and difenoconazole on survival and reproduction of the springtail Folsomia candida

Environmental Pollution, Journal Year: 2018, Volume and Issue: 244, P. 342 - 350

Published: Oct. 16, 2018

Language: Английский

---

CRISPR/Cas9-Mediated Vitellogenin Receptor Knockout Leads to Functional Deficiency in the Reproductive Development of Plutella xylostella

Frontiers in Physiology, Journal Year: 2020, Volume and Issue: 10

Published: Jan. 23, 2020

The vitellogenin receptor (VgR) belongs to the low-density lipoprotein (LDLR) gene superfamily and plays an indispensable role in Vg transport, yolk deposition, oocyte development. For this reason, it has become a promising target for pest control. involvement of VgR transport reproductive functions remains unclear diamondback moths, Plutella xylostella (L.), destructive cruciferous crops. Here, we cloned identified complete cDNA sequence P. VgR, which encoded 1805 amino acid residues contained four conserved domains LDLR superfamily. PxVgR was mainly expressed female adults, more specifically ovary. protein also showed similar expression profile with transcript. CRISPR/Cas9-mediated knockout created homozygous mutant 5-bp-nucleotide deletion PxVgR. deficiency detected ovaries eggs individuals. still individuals, but decreased level. However, PxVg transcripts were not significantly affected by knockout. Knockout resulted shorter ovarioles newly emerged females. No significant difference between wild individuals terms number laid first 3 days after mating. loss smaller whiter lower egg hatching rate. This study represents report on ovary development, oviposition, embryonic development using CRISPR/Cas9 technology. lays foundation understanding molecular mechanisms reproduction, making use as potential genetic-based better control xylostella.

Language: Английский

---