Network-Based Analysis of Virulence Factors for Uncovering Aeromonas Veronii Pathogenesis DOI Creative Commons
Hong Li, Xiang Ma,

Yanqiong Tang

et al.

Research Square (Research Square), Journal Year: 2021, Volume and Issue: unknown

Published: April 26, 2021

Abstract Background: Aeromonas veronii is a pathogen that causes serious harm to aquaculture. Virulence factors are its pathogenic basis, which could promote pathogens colonize the host, evade host defense and so on. But because experimental verification of virulence time-consuming laborious, number known limited. In this past, most studies only focused on single factor, resulting biased interpretation for pathogenesis. Results: study, protein-protein interaction (PPI) network at genome-wide scale A. was first constructed. Then, were predicted mapped network. Topological characteristics analyzed. The results showed had higher degree betweenness centrality than other proteins in particular, tended interact with each enriched two modules. One modules mainly consisted histidine kinases, response regulators, diguanylate cyclases phosphodiesterases, played important roles two-component regulatory systems synthesis degradation cyclic-diGMP. Furthermore, an interspecies PPI between Oreochromis niloticus also structures interacting sites added By analyzing network, we found competitively bind some shared by different proteins. Drugs be designed target these further prevent interfere pathways. Conclusions: Our indicated regulated involving signal transduction pathway manipulate biological processes mimicking binding deepened understanding pathogenesis theoretical significance designing targeted antibacterial drugs.

Language: Английский

Understanding the Dynamics of Blast Resistance in Rice-Magnaporthe oryzae Interactions DOI Creative Commons

Basavantraya N. Devanna,

Priyanka Jain, Amolkumar U. Solanke

et al.

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(6), P. 584 - 584

Published: May 30, 2022

Rice is a global food grain crop for more than one-third of the human population and source nutritional security. production subjected to various stresses; blast disease caused by Magnaporthe oryzae one major biotic stresses that has potential destroy total under severe conditions. In present review, we discuss importance rice in future context, genomics molecular biology pathogen rice, interplay between rice–M. interaction governed different gene models. We also elaborated detail on M. effector Avr genes, role noncoding RNAs development. Further, resistance QTLs; (R) genes; alleles identified, cloned, characterized are discussed. utilization QTLs R genes through conventional breeding transgenic approaches. Finally, review demonstrated examples applications latest genome-editing tools understanding managing rice.

Language: Английский

Citations

82
Cell surface immune receptors: the guardians of the plant’s extracellular spaces DOI Creative Commons
K. Kanyuka, J. J. Rudd

Current Opinion in Plant Biology, Journal Year: 2019, Volume and Issue: 50, P. 1 - 8

Published: March 9, 2019

Since the original 'Zigzag model', several iterations have been proposed to reconcile both Pattern Triggered Immunity (PTI) and Effector (ETI) branches of plant immune system. The recent cloning new disease resistance genes, functioning in gene-for-gene interactions, which structurally resemble cell surface broad spectrum Recognition Receptors, further blurred distinctions between PTI ETI immunity. In an attempt simplify existing conceptual models, we, herein, propose a scheme based on spatial localization key proteins (receptors) function induce responses. We believe this 'Spatial Invasion model' will prove useful for understanding how receptors interact with different pathogen types peripherally or totally invade cells, colonize solely extracellularly switch locations during successful infection.

Language: Английский

Citations

125
Suppression of Drosophila antifungal immunity by a parasite effector via blocking GNBP3 and GNBP-like 3, the dual receptors for β-glucans DOI Creative Commons

Mengting Lu,

Dongxiang Wei,

Junmei Shang

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(1), P. 113642 - 113642

Published: Jan. 1, 2024

The tactics used by animal pathogens to combat host immunity are largely unclear. Here, we report the depiction of virulence-required effector Tge1 deployed entomopathogen Metarhizium robertsii suppress Drosophila antifungal immunity. can target both GNBP3 and GNBP-like 3 (GL3), latter bind β-glucans like GNBP3, whereas glucan binding receptors be attenuated Tge1. As opposed surveillance GL3 is inducible in depending on Toll pathway via a positive feedback loop mechanism. Losses genes result deregulations protease cascade, Spätzle maturation, antimicrobial gene expressions upon fungal challenges. Fly survival assays confirm that plays more essential role than combating infections. In addition evidencing gene-for-gene interactions between fungi insects, our data advance insights into

Language: Английский

Citations

12
Exploring unconventional attributes of red blood cells and their potential applications in biomedicine DOI Creative Commons
Alkmini T. Anastasiadi,

Vasiliki-Zoi Arvaniti,

Krystalyn E. Hudson

et al.

Protein & Cell, Journal Year: 2024, Volume and Issue: 15(5), P. 315 - 330

Published: Jan. 25, 2024

Language: Английский

Citations

11
In vivo analysis of influenza A mRNA secondary structures identifies critical regulatory motifs DOI Creative Commons

Lisa Marie Simon,

Edoardo Morandi, Anna Luganini

et al.

Nucleic Acids Research, Journal Year: 2019, Volume and Issue: 47(13), P. 7003 - 7017

Published: April 23, 2019

The influenza A virus (IAV) is a continuous health threat to humans as well animals due its recurring epidemics and pandemics. IAV genome segmented the eight negative-sense viral RNAs (vRNAs) are transcribed into positive sense complementary (cRNAs) messenger (mRNAs) inside infected host cells. role for secondary structure of mRNAs has been hypothesized debated many years, but knowledge on adopt in vivo currently missing. Here we solve, first time, living We demonstrate that, compared vitro refolded structure, less structured exhibit specific locally stable elements. Moreover, show that targeted disruption these high-confidence domains results an extraordinary attenuation replicative capacity. Collectively, our data provide comprehensive map structural landscape mRNAs, hence providing means development new RNA-targeted antivirals.

Language: Английский

Citations

65
SWEET genes and TAL effectors for disease resistance in plants: Present status and future prospects DOI Creative Commons
P. K. Gupta, H. S. Balyan, Tinku Gautam

et al.

Molecular Plant Pathology, Journal Year: 2021, Volume and Issue: 22(8), P. 1014 - 1026

Published: June 2, 2021

Abstract SWEET genes encode sugar transporter proteins and often function as susceptibility (S) genes. Consequently, the recessive alleles of these provide resistance. This review summarizes available literature on molecular basis role (as S genes) in host corresponding transcription activator‐like effectors (TALEs) secreted by pathogen. The has four major sections, which follow a brief introduction: first part gives some details about occurrence evolution approximately 30 plant species; second systems where (a) with without TALEs (b) cause different diseases; third information along interfering/truncated pathogens; this section also effector‐binding elements (EBEs) promoters either or Executor R genes; code that is used for binding to EBEs described section; fourth approaches are being can be future exploiting developing disease‐resistant cultivars. concludes giving conclusions possibilities using cultivars approaches, including conventional breeding genome editing.

Language: Английский

Citations

48
The Mycobacterium tuberculosis PE_PGRS Protein Family Acts as an Immunological Decoy to Subvert Host Immune Response DOI Open Access
Tarina Sharma,

Anwar Alam,

Aquib Ehtram

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(1), P. 525 - 525

Published: Jan. 4, 2022

Mycobacterium tuberculosis (M.tb) is a successful pathogen that can reside within the alveolar macrophages of host and survive in latent stage. The has evolved developed multiple strategies to resist immune responses. M.tb escapes from macrophage through evasion or subversion effector functions. genome codes for PE/PPE/PE_PGRS proteins, which are intrinsically disordered, redundant antigenic nature. These proteins perform functions intensify virulence competence majorly by modulating responses, thereby affecting mediated clearance pathogen. highly repetitive, nature provide critical edge over other terms imparting higher level also as decoy molecule masks effect molecules, immuno-surveillance. An understanding how these subvert immunological machinery may add current knowledge about pathogenesis. This help redirecting our tackling infections.

Language: Английский

Citations

36
Good Riddance? Breaking Disease Susceptibility in the Era of New Breeding Technologies DOI Creative Commons
Stefan Engelhardt, Remco Stam, Ralph Hückelhoven

et al.

Agronomy, Journal Year: 2018, Volume and Issue: 8(7), P. 114 - 114

Published: July 5, 2018

Despite a high abundance and diversity of natural plant pathogens, disease susceptibility is rare. In agriculture however, epidemics often occur when virulent pathogens successfully overcome immunity single genotype grown in monoculture. Disease are partially controlled by chemical genetic protection, but pathogen populations show potential to adapt new cultivars or control agents. Therefore, strategies breeding biotechnology required obtain durable resistance. Generating exploiting loss one the recent strategies. Better understanding host genes (S) technologies now enable targeted mutation S for protection. Here we summarize biological functions factors both conventional DNA nuclease-based exploitation genes. We further discuss trade-offs whether can provide

Language: Английский

Citations

50
Hce2 domain‐containing effectors contribute to the full virulence ofValsa maliin a redundant manner DOI Creative Commons
Mian Zhang, Shichang Xie,

Yuhuan Zhao

et al.

Molecular Plant Pathology, Journal Year: 2019, Volume and Issue: 20(6), P. 843 - 856

Published: March 26, 2019

Valsa mali is the causal agent of apple canker, a destructive disease in East Asia. Effector proteins play important roles virulence phytopathogenic fungi, and we identified five Hce2 domain-containing effectors (VmHEP1, VmHEP2, VmHEP3, VmHEP4 VmHEP5) from V. genome. Amongst these, VmHEP1 VmHEP2 were found to be up-regulated during early infection stage was also as cell death inducer through its transient expression Nicotiana benthamiana. Although deletion each single VmHEP gene did not lead reduction virulence, double-deletion notably attenuated both twigs leaves. An evolutionary analysis revealed that are two paralogues, under purifying selection. located next other on chromosome 11 tandem genes with only 604 bp physical distance. Interestingly, promoted and, vice versa, VmHEP1. The present results provide insights into functions acting factors mali, new perspective regarding contribution fungi.

Language: Английский

Citations

31
Genome-wide identification of the NLR gene family in Haynaldia villosa by SMRT-RenSeq DOI Creative Commons

Zhenpu Huang,

Fangyuan Qiao,

Boming Yang

et al.

BMC Genomics, Journal Year: 2022, Volume and Issue: 23(1)

Published: Feb. 10, 2022

Nucleotide-binding and leucine-rich repeat (NLR) genes have attracted wide attention due to their crucial role in protecting plants from pathogens. SMRT-RenSeq, combining PacBio sequencing after resistance gene enrichment (RenSeq), is a powerful method for selectively capturing full-length NLRs. Haynaldia villosa, wild grass species with proven potential wheat improvement, confers multiple diseases. So, genome-wide identification of the NLR family villosa by SMRT-RenSeq can facilitate disease exploration.In this study, was performed identify complement H. villosa. In total, 1320 NLRs were annotated 1169 contigs, including 772 complete All phylogenetically analyzed 11 main clades special characteristics derived. could be captured high efficiency when aligned cloned R genes, cluster expansion some specific loci observed. The physical location individual chromosomes showed perfect homoeologous relationship group 1, 2, 3, 5 6 other Triticeae species, however, physically located on 4VL largely silico predicted 7. Fifteen types integrated domains (IDs) 52 NLRs, Kelch B3 NLR-IDs found expanded while DUF948, NAM-associated PRT_C detected as unique implying new emergence diverged species.SMRT-RenSeq tool using baits evolutionary related reference sequences. availability provide valuable library mining transfer into wheat.

Language: Английский

Citations

18