Emerging Roles of SWEET Sugar Transporters in Plant Development and Abiotic Stress Responses

Cells, Journal Year: 2022, Volume and Issue: 11(8), P. 1303 - 1303

Published: April 12, 2022

Sugars are the major source of energy in living organisms and play important roles osmotic regulation, cell signaling storage. SWEETs (Sugars Will Eventually be Exported Transporters) most recent family sugar transporters that function as uniporters, facilitating diffusion molecules across membranes. In plants, multiple physiological processes including phloem loading, senescence, pollen nutrition, grain filling, nectar secretion, abiotic (drought, heat, cold, salinity) biotic stress regulation. this review, we summarized role SWEET plant development stress. The gene expression dynamics various under stresses different species also discussed. Finally, discuss utilization genome editing tools (TALENs CRISPR/Cas9) to engineer genes can facilitate trait improvement. Overall, advancements on highlighted, which could used for crop improvement tolerance.

Language: Английский

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Extracellular niche establishment by plant pathogens

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: 22(6), P. 360 - 372

Published: Jan. 8, 2024

Language: Английский

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Structure, evolution, and roles of SWEET proteins in growth and stress responses in plants

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 263, P. 130441 - 130441

Published: Feb. 28, 2024

Language: Английский

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CRISPR/Cas9-generated mutations in a sugar transporter gene reduce cassava susceptibility to bacterial blight

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 195(4), P. 2566 - 2578

Published: May 3, 2024

Bacteria from the genus Xanthomonas are prolific phytopathogens that elicit disease in over 400 plant species. Xanthomonads carry a repertoire of specialized proteins called transcription activator-like (TAL) effectors promote and pathogen virulence by inducing expression host susceptibility (S) genes. phaseoli pv. manihotis (Xpm) causes bacterial blight on staple food crop cassava (Manihot esculenta Crantz). The Xpm effector TAL20 induces ectopic S gene Manihot Sugars Will Eventually be Exported Transporter 10a (MeSWEET10a), which encodes sugar transporter contributes to (CBB) susceptibility. We used CRISPR/Cas9 generate multiple lines with edits MeSWEET10a binding site and/or coding sequence. In several regenerated lines, was no longer induced Xpm, these cases, we observed reduced CBB symptoms post infection. Because is expressed flowers, further characterized reproductive capability promoter sequence mutants. Lines were crossed themselves wild-type plants. results indicated female, but not male, flowers critical produce viable F1 seed. case mutations left intact, progeny recovered. Taken together, demonstrate blocking induction strategy for decreasing ideal will contain block TAL while leaving endogenous unaltered.

Language: Английский

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SWEET genes and TAL effectors for disease resistance in plants: Present status and future prospects

Molecular Plant Pathology, Journal Year: 2021, Volume and Issue: 22(8), P. 1014 - 1026

Published: June 2, 2021

Abstract SWEET genes encode sugar transporter proteins and often function as susceptibility (S) genes. Consequently, the recessive alleles of these provide resistance. This review summarizes available literature on molecular basis role (as S genes) in host corresponding transcription activator‐like effectors (TALEs) secreted by pathogen. The has four major sections, which follow a brief introduction: first part gives some details about occurrence evolution approximately 30 plant species; second systems where (a) with without TALEs (b) cause different diseases; third information along interfering/truncated pathogens; this section also effector‐binding elements (EBEs) promoters either or Executor R genes; code that is used for binding to EBEs described section; fourth approaches are being can be future exploiting developing disease‐resistant cultivars. concludes giving conclusions possibilities using cultivars approaches, including conventional breeding genome editing.

Language: Английский

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Physiological implications of SWEETs in plants and their potential applications in improving source–sink relationships for enhanced yield

Plant Biotechnology Journal, Journal Year: 2022, Volume and Issue: 21(8), P. 1528 - 1541

Published: Dec. 19, 2022

The sugars will eventually be exported transporters (SWEET) family of in plants is identified as a novel class sugar carriers capable transporting sugars, alcohols and hormones. Functioning intercellular transport, SWEETs influence wide range physiologically important processes. regulate the development sink organs by providing nutritional support from source leaves, responses to abiotic stresses maintaining intracellular concentrations, host-pathogen interactions through modulation apoplastic levels. Many bacterial fungal pathogens activate expression SWEET genes species such rice Arabidopsis gain access nutrients that virulence. genetic manipulation has led generation blight (BB)-resistant varieties. Similarly, while overexpression involved sucrose export leaves pathogenesis growth retardation yield penalties, overexpressing show improved disease resistance. Such findings demonstrate complex functions stress tolerance. Here, we review importance plant-pathogen source-sink We highlight possible applications crop improvement programmes aimed at improving strengths for enhancing sustainability yield. discuss how adverse effects on plant may overcome.

Language: Английский

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Quantitative disease resistance: Multifaceted players in plant defense

Journal of Integrative Plant Biology, Journal Year: 2022, Volume and Issue: 65(2), P. 594 - 610

Published: Nov. 30, 2022

Abstract In contrast to large‐effect qualitative disease resistance, quantitative resistance (QDR) exhibits partial and generally durable has been extensively utilized in crop breeding. The molecular mechanisms underlying QDR remain largely unknown but considerable progress made this area recent years. review, we summarize the genes that have associated with plant their biological functions. Many belong canonical gene categories predicted functions pathogen perception, signal transduction, phytohormone homeostasis, metabolite transport biosynthesis, epigenetic regulation. However, other “atypical” are be involved processes not commonly such as vesicle trafficking, chaperones, others. This diversity of function for contrasts which is often based on actions nucleotide‐binding leucine‐rich repeat (NLR) proteins. An understanding effective against classes pathogens will enable more deployment produce durably resistant, resilient crops.

Language: Английский

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Promoter editing for the genetic improvement of crops

Journal of Experimental Botany, Journal Year: 2023, Volume and Issue: 74(15), P. 4349 - 4366

Published: May 19, 2023

Abstract Gene expression plays a fundamental role in the regulation of agronomically important traits crop plants. The genetic manipulation plant promoters through genome editing has emerged as an effective strategy to create favorable crops by altering pattern pertinent genes. Promoter can be applied directed manner, where nucleotide sequences associated with are precisely generated. Alternatively, promoter also exploited random mutagenic approach generate novel variations within designated promoter, from which elite alleles selected based on their phenotypic effects. Pioneering studies have demonstrated potential engineering well mining valuable for breeding. In this review, we provide update application increased yield, enhanced tolerance biotic and abiotic stresses, improved quality. We discuss several remaining technical bottlenecks how may better employed improvement future.

Language: Английский

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Phytophthora sojae boosts host trehalose accumulation to acquire carbon and initiate infection

Nature Microbiology, Journal Year: 2023, Volume and Issue: 8(8), P. 1561 - 1573

Published: June 29, 2023

Language: Английский

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Advancing crop disease resistance through genome editing: a promising approach for enhancing agricultural production

Frontiers in Genome Editing, Journal Year: 2024, Volume and Issue: 6

Published: June 26, 2024

Modern agriculture has encountered several challenges in achieving constant yield stability especially due to disease outbreaks and lack of long-term disease-resistant crop cultivars. In the past, economically important crops had a major impact on food security economy. On other hand climate-driven emergence new pathovars or changes their host specificity further poses serious threat sustainable agriculture. At present, chemical-based control strategies are frequently used microbial pathogens pests, but they have detrimental environment also resulted development resistant phyto-pathogens. As replacement, cultivating engineered can help minimize negative regular pesticides environment. Although traditional breeding genetic engineering been instrumental improvement certain limitations such as labour intensity, time consumption, low efficiency. this regard, genome editing emerged one potential tools for improving resistance by targeting multiple traits with more accuracy For instance, techniques, CRISPR/Cas9, CRISPR/Cas13, base editing, TALENs, ZFNs, meganucleases, proved successful through targeted mutagenesis, gene knockouts, knockdowns, modifications, activation target genes. CRISPR/Cas9 is unique among these techniques because its remarkable efficacy, risk off-target repercussions, ease use. Some primary targets developing CRISPR-mediated host-susceptibility genes (the S method), (R genes) pathogen material that prevents development, broad-spectrum resistance. The use methods notably ameliorate transform agricultural practices future. This review highlights phyto-pathogens productivity. Next, we discussed while focusing editing. We provided an update accomplishments improve against bacterial, fungal viral different systems. Finally, highlighted future systems enhancing

Language: Английский

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