Avoiding alkaline taste through ionotropic receptors

iScience, Journal Year: 2024, Volume and Issue: 27(6), P. 110087 - 110087

Published: May 23, 2024

Taste organs contain distinct gustatory receptors that help organisms differentiate between nourishing and potentially harmful foods. The detection of high pH levels plays a crucial role in food selection, but the specific responsible for perceiving elevated foods have remained unknown. By using

Language: Английский

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Tastant-receptor interactions: insights from the fruit fly

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: April 11, 2024

Across species, taste provides important chemical information about potential food sources and the surrounding environment. As details chemicals receptors responsible for gustation are discovered, a complex view of system is emerging with significant contributions from research using fruit fly, Drosophila melanogaster , as model organism. In this brief review, we summarize recent advances in their relevance to more broadly. Our goal highlight molecular mechanisms underlying first step gustatory circuits: ligand-receptor interactions primary cells. After an introduction how it encodes canonical modalities sweet, bitter, salty, describe insights into nature carboxylic acid amino detection context sour umami taste, respectively. analysis extends non-canonical including metals, fatty acids, bacterial components, highlights unexpected signaling pathways that have recently been identified Comparing intricate cellular underpinnings ligands detected vivo flies reveals both specific promiscuous receptor selectivity encoding. Throughout compare contextualize these findings mammalian not only emphasize conservation chemosensory systems, but demonstrate power organism elucidating neurobiology feeding.

Language: Английский

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Identification and function analysis of two new gustatory receptors related to silkworm monophagy

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Silkworm is a typical monophagous insect that can only feed on fresh mulberry leaves. The mechanism for this nature not fully understood. One bitter gustatory receptor (GR) GR66 located the maxilla of mouthpart has been reported to be an important factor influencing feeding preference silkworm. However, mutants nonhost plant was very low, suggesting other factors related silkworm monophagy need further explored. In study, 10 GRs were screened out based their specific high expression in silkworm, and 4 most expressed (GR15, GR43, GR69, GR66) knocked by clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated nuclease 9. Feeding experiments showed except GR69-/-, host range GR43-/-, GR15-/-, GR66-/- expanded remarkably, both GR43-/- revealed highest plants. Moreover, strict leaves almost eliminated double mutant silkworms GR66-/-. These results imply it likely variety different are involved determining larvae. addition, GR15 mutation significantly inhibited ovarian development, resulting significant decrease oviposition. findings enhance our understanding provide possibility molecular breeding polyphagous future.

Language: Английский

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Harnessing Insect Chemosensory and Mechanosensory Receptors Involved in Feeding for Precision Pest Management

Life, Journal Year: 2025, Volume and Issue: 15(1), P. 110 - 110

Published: Jan. 16, 2025

Chemosensation and mechanosensation are vital to insects’ survival behavior, shaping critical physiological processes such as feeding, metabolism, mating, reproduction. During insects rely on diverse chemosensory mechanosensory receptors distinguish between nutritious harmful substances, enabling them select suitable food sources while avoiding toxins. These distributed across various body parts, allowing detect environmental cues about quality adjust their behaviors accordingly. A deeper understanding of insect sensory physiology, especially during not only enhances our knowledge biology but also offers significant opportunities for practical applications. This review highlights recent advancements in research feeding-related receptors, covering a wide range species, from the model organism Drosophila melanogaster agricultural human pests. Additionally, this examines potential targeting precision pest control. Disrupting feeding reproduction emerges promising strategy management. By interfering with these essential behaviors, we can effectively control populations minimizing impacts promoting ecological balance.

Language: Английский

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Artificial sweeteners differentially activate sweet and bitter gustatory neurons in Drosophila

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

ABSTRACT Artificial sweeteners are highly sweet, non-nutritive compounds that have become increasingly popular over recent decades despite research suggesting their consumption has unintended consequences. Specifically, there is evidence some of these chemicals interact with bitter taste receptors, implying likely generate complex chemosensory signals. Here, we report the basic sensory characteristics in Drosophila , a common model system used to study impacts diet, and find all noncaloric inhibited appetitive feeding responses at higher concentrations. At cellular level, found sucralose rebaudioside A co-activated sweet gustatory receptor neurons (GRNs), two populations reciprocally impact behavior, while aspartame only activated cells. We assessed behavioral co-activation low concentrations signal high aversion. Finally, silencing GRNs reduced aversive elicited by significantly increased behaviors. Together, conclude artificial more than “sweetness” alone, this behaviorally relevant effects on may help flies flexibly respond unique compounds.

Language: Английский

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Cholesterol taste avoidance in Drosophila melanogaster

Published: April 7, 2025

The question as to whether animals taste cholesterol is not resolved. This study investigates the fruit fly, Drosophila melanogaster , capable of detecting through their gustatory system. We found that flies are indifferent low levels and avoid higher levels. avoidance mediated by receptor neurons (GRNs), demonstrating can cholesterol. responsive GRNs comprise a subset also respond bitter substances. Cholesterol detection depends on five ionotropic (IR) family members, disrupting any these genes impairs flies’ ability Ectopic expressions IRs in reveals two classes receptors, each with three shared one unique subunit. Additionally, expressing receptors sugar-responsive confers attraction cholesterol, GRNs.

Language: Английский

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Cholesterol taste avoidance in Drosophila melanogaster

Published: April 7, 2025

The question as to whether animals taste cholesterol is not resolved. This study investigates the fruit fly, Drosophila melanogaster, capable of detecting through their gustatory system. We found that flies are indifferent low levels and avoid higher levels. avoidance mediated by receptor neurons (GRNs), demonstrating can cholesterol. cholesterol-responsive GRNs comprise a subset also responds bitter substances. Cholesterol detection depends on five ionotropic (IR) family members, disrupting any these genes impairs flies' ability Ectopic expressions IRs in reveals two classes receptors, each with three shared one unique subunit. Additionally, expressing receptors sugar-responsive confers attraction cholesterol, GRNs.

Language: Английский

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Cholesterol taste avoidance in Drosophila melanogaster

eLife, Journal Year: 2025, Volume and Issue: 14

Published: April 17, 2025

The question as to whether animals taste cholesterol is not resolved. This study investigates the fruit fly, Drosophila melanogaster , capable of detecting through their gustatory system. We found that flies are indifferent low levels and avoid higher levels. avoidance mediated by receptor neurons (GRNs), demonstrating can cholesterol. cholesterol-responsive GRNs comprise a subset also responds bitter substances. Cholesterol detection depends on five ionotropic (IR) family members, disrupting any these genes impairs flies' ability Ectopic expressions IRs in reveals two classes receptors, each with three shared one unique subunit. Additionally, expressing receptors sugar-responsive confers attraction cholesterol, GRNs.

Language: Английский

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The power of Drosophila genetics in studying insect toxicology and chemical ecology

Crop Health, Journal Year: 2023, Volume and Issue: 1(1)

Published: Nov. 27, 2023

Language: Английский

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Evolution of fatty acid taste in drosophilids

Cell Reports, Journal Year: 2023, Volume and Issue: 42(10), P. 113297 - 113297

Published: Oct. 1, 2023

Comparative studies of related but ecologically distinct species can reveal how the nervous system evolves to drive behaviors that are particularly suited certain environments. Drosophila melanogaster is a generalist feeds and oviposits on most overripe fruits. A sibling species, D. sechellia, an obligate specialist Morinda citrifolia (noni) fruit, which rich in fatty acids (FAs). To understand evolution noni taste preference, we characterized behavioral cellular responses noni-associated FAs three drosophilids. We find mixtures sugar evoke strong aversion not sechellia. Surveys sensory FA- species-specific differences at least two mechanisms—bitter neuron activation sweet inhibition–that correlate with shifts preference. Chemoreceptor mutant analysis predicts multiple genetic changes account for gustatory preference

Language: Английский

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