Neuronal PAS domain 1 identifies a major subpopulation of wakefulness-promoting GABAergic neurons in basal forebrain DOI Open Access
Timothy A. Troppoli, Chun Yang, Fumi Katsuki

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 11, 2023

ABSTRACT Here we describe a novel group of basal forebrain (BF) neurons expressing neuronal PAS domain 1 (Npas1), developmental transcription factor linked to neuropsychiatric disorders. Immunohistochemical staining in Npas1-cre-2A-TdTomato mice revealed BF Npas1 + are distinct from well-studied parvalbumin or cholinergic neurons. GAD67-GFP knock-in confirmed that the vast majority GABAergic, with minimal colocalization glutamatergic vGlut1-cre-tdTomato vGlut2-cre-tdTomato mice. The density was high, 5-6 times neighboring cholinergic, Anterograde tracing identified prominent projections brain regions involved sleep-wake control, motivated behaviors and olfaction such as lateral hypothalamus, habenula, nucleus accumbens shell, ventral tegmental area olfactory bulb. Chemogenetic activation light (inactive) period increased amount wakefulness latency sleep for 2-3 hr, due an increase long wake bouts short NREM bouts. Non-REM slow-wave (0-1.5 Hz) sigma (9-15 power, well spindle density, amplitude duration, were reduced, reminiscent findings several Together previous implicating stress responsiveness, anatomical effect activating them suggest possible role motivationally-driven stress-induced insomnia. Identification this major subpopulation GABAergic will facilitate studies their disorders, dementia other conditions involving BF. SIGNIFICANCE STATEMENT We characterize mouse subset more numerous than glutamate target areas arousal, motivation olfaction. Activation slow waves spindles reduced wake-promoting allow insomnia,

Language: Английский

Bulbar projecting subcortical GABAergic neurons send collateral branches extensively and selectively to primary olfactory cortical regions DOI

Chelsea Hook,

Adam C. Puché

The Journal of Comparative Neurology, Journal Year: 2022, Volume and Issue: 531(3), P. 451 - 460

Published: Dec. 3, 2022

Abstract Circuit operations of the olfactory bulb are modulated by higher order projections from multiple regions, many which themselves targets bulbar output. Multiple glutamatergic regions project to bulb, including anterior nucleus (AON), prefrontal cortex (PFC), piriform (PC), entorhinal (EC), and tenia tecta (TT). In contrast, only one region provides GABAergic bulb. These GABA neurons located in horizontal limb diagonal band Broca extending posteriorly through magnocellular preoptic lateral However, it was unclear whether projecting collaterallize other brain regions. To address this, we mapped collateral using intersectional strategies viral traditional tract tracers. This approach revealed show remarkable specificity targeting primary cortical exhibiting abundant into accessory AON, PFC, PC, TT. The "nonolfactory" receiving sparse connectivity medial orbital cortex. suggests that basal forebrain inhibitory feedback also modulates areas prominent projection Thus, may be simultaneously modulating both synaptic processing information associational We hypothesize these a regulator entire system.

Language: Английский

Citations

4
Deficits in odor discrimination versus odor identification in patients with schizophrenia and negative correlations with GABAergic and DNA methyltransferase mRNAs in lymphocytes DOI Creative Commons
Robert C. Smith, Henry Sershen,

Mary Youssef

et al.

Frontiers in Psychiatry, Journal Year: 2023, Volume and Issue: 14

Published: March 28, 2023

People with schizophrenia have been reported to show deficits in tests of olfactory function. DNA methylation and GABAergic input implicated biochemical processes controlling odor animal studies, but this has not investigated human studies.In a study measures mRNAs lymphocytes, we also measured identification discrimination the Sniffin' Sticks battery 58 patients chronic (CSZ) 48 controls. lymphocytes were assessed by qPCR using TaqManTM probes. Cognition was MATRICS (Measurement Treatment Research Improve Schizophrenia) CSZ controls, symptoms PANSS scale (Positive Negative Symptom Scale). The relationships mRNA, cognition, explored correlation analysis. Variables which significantly differentiated from controls logistic regression.Overall, showed (P≤.001) lower scores on compared moderate effect size, no difference identification. Deficits discrimination, standardly many prior strongly In regression analysis, identification, significant variable predicting versus control class membership. This is first report relationship between blood cells subjects. There negative correlations enzymes mRNAs. Lower associated cognitive CSZ, symptom scores, while higher PANNS Excitement factor.Odor more powerful than discriminating should be used regularly as an measure studies schizophrenia. substantive meaning mRNA variables peripheral needs investigation comparison results neural tissue.

Language: Английский

Citations

2
The Response Dynamics and Function of Cholinergic and GABAergic Neurons in the Basal Forebrain During Olfactory Learning DOI Creative Commons

Ping Zhou,

Penglai Liu,

Ying Zhang

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2022, Volume and Issue: 16

Published: July 27, 2022

Modulation of neural circuits is essential for flexible sensory perception and decision-making in a changing environment. Cholinergic GABAergic projections to the olfactory system from horizontal limb diagonal band Broca (HDB) basal forebrain are crucial odor detection learning. Although studies have demonstrated that HDB neurons respond during learning, how cholinergic differ their response dynamics roles learning remains unclear. In this study, we examined profiles these two subpopulations passive exposure associative We show excitatory responses both tended habituate repeated exposure. However, while habituated were also observed go-go task, there was no such habituation neurons. Moreover, S+ S- trials diverged once mice learned go/no-go task. Furthermore, chemogenetic inactivation impaired discrimination. Together, findings suggest reflect attention positive reinforcement may regulate discrimination via top-down inputs system.

Language: Английский

Citations

3
Neuronal network-based biomimetic chip for long-term detection of olfactory dysfunction model in early-stage Alzheimer's disease DOI
Mengxue Liu, Changming Chen,

Keqiang Gao

et al.

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 216, P. 114619 - 114619

Published: Aug. 7, 2022

Language: Английский

Citations

3
Cell type-specific and frequency-dependent centrifugal modulation in olfactory bulb output neurons in vivo DOI
Adam C. Puché,

Chelsea Hook,

Fu‐Wen Zhou

et al.

Journal of Neurophysiology, Journal Year: 2024, Volume and Issue: 131(6), P. 1226 - 1239

Published: May 1, 2024

Mitral/tufted cells (M/TCs) form complex local circuits with interneurons in the olfactory bulb and are powerfully inhibited by these interneurons. The horizontal limb of diagonal band Broca (HDB), only GABAergic/inhibitory source centrifugal circuit bulb, is known to target interneurons, we have shown targeting also glutamatergic neurons vitro. However, net efficacy under different patterns activation vivo relative balance between various targeted intact was focus this study. Here channelrhodopsin-2 (ChR2) expressed HDB GABAergic investigate short-term plasticity HDB-activated disinhibitory rebound excitation M/TCs. Optical increased spontaneous M/TC firing without odor presentation odor-evoked firing. induced (burst or cluster spiking) all classes This frequency dependent, facilitation at higher stimulation (5 Hz above). frequency-dependent regulation more potent deeper layer M/TCs compared superficial In neural inhibition plays a critical functional role, patterned input-dependent inhibitory inputs may help maintain precise across populations output environmental odors, putatively sharpen enhancement tuning specificity individual odors.

Language: Английский

Citations

0
Basal forebrain modulation of olfactory coding in vivo DOI Creative Commons
Juan Pablo Venegas, Marcela Díaz,

Laura Orellana-Garcia

et al.

International journal of psychological research, Journal Year: 2023, Volume and Issue: 16(2), P. 62 - 86

Published: Aug. 23, 2023

Sensory perception is one of the most fundamental brain functions, allowing individuals to properly interact and adapt a constantly changing environment. This process requires integration bottom-up topdown neuronal activity, which centrally mediated by basal forebrain, region that has been linked series cognitive processes such as attention alertness. Here, we review latest research using optogenetic approaches in rodents vivo electrophysiological recordings are shedding light on role this region, regulating olfactory processing decisionmaking. Moreover, summarize evidence highlighting anatomical physiological differences forebrain with autism spectrum disorder, could underpin sensory abnormalities they exhibit, propose line potential opportunity understand neurobiological basis disorder.La percepción sensorial es una de las funciones cerebrales más fundamentales, permitiendo los individuos interactuar manera apropiada con el entorno y adaptarse un ambiente en constante cambio. Este proceso requiere la integración actividad ascendente descendente, que mediada por cerebro (BF), región cerebral ha sido asociada serie procesos cognitivos, como estados atención alerta.En este trabajo revisamos últimas investigaciones han utilizado optogenética registros electrofisiológicos iluminado rol del BF procesamiento olfatorio toma decisiones. Además, resumimos literatura destaca alteraciones fisiológicas anatómicas trastornos espectro autista, podrían subyacer anormalidades presentan, proponemos esta línea investigación posible oportunidad para entender bases neurobiológicas trastorno.

Citations

1
Parvalbumin-expressing basal forebrain neurons mediate learning from negative experience DOI Creative Commons

Panna Hegedüs,

B. Király, Dániel Schlingloff

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 7, 2024

Abstract Parvalbumin (PV)-expressing GABAergic neurons of the basal forebrain (BFPVNs) were proposed to serve as a rapid and transient arousal system, yet their exact role in awake behaviors remains unclear. We performed bulk calcium measurements electrophysiology with optogenetic tagging from horizontal limb diagonal band Broca (HDB) while male mice performing an associative learning task. BFPVNs responded distinctive, phasic activation punishment, but showed slower delayed responses reward outcome-predicting stimuli. Optogenetic inhibition during punishment impaired formation cue-outcome associations, suggesting causal learning. received strong inputs hypothalamus, septal complex median raphe region, they synapsed on diverse cell types key limbic structures, where broadcasted information about aversive propose that arousing effect is recruited by stimuli crucial functions.

Language: Английский

Citations

0
Olfactory integration and odor perception DOI
Patricia Duchamp‐Viret, Nicola Kuczewski, Christine Baly

et al.

Elsevier eBooks, Journal Year: 2022, Volume and Issue: unknown, P. 149 - 204

Published: Aug. 19, 2022

Language: Английский

Citations

2
The effects of acamprosate on maternal and neonatal outcomes in a mouse model of alcohol use disorders DOI
Ebony Quintrell, Caitlin S. Wyrwoll, Tim Rosenow

et al.

Physiology & Behavior, Journal Year: 2022, Volume and Issue: 259, P. 114037 - 114037

Published: Nov. 23, 2022

Language: Английский

Citations

2
Dynamic cholinergic tone in the basal forebrain reflects reward-seeking and reinforcement during olfactory behavior DOI Creative Commons

Elizabeth Hanson,

Katie L. Brandel-Ankrapp, Benjamin R. Arenkiel

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2020, Volume and Issue: unknown

Published: Dec. 2, 2020

Abstract Sensory perception underlies how we internalize and interact with the external world. In order to adapt changing circumstances interpret signals in a variety of contexts, sensation needs be reliable, but sensory input flexible. An important mediator this flexibility is top-down regulation from cholinergic basal forebrain. Basal forebrain projection neurons serve as pacemakers gatekeepers for downstream neural networks, modulating circuit activity across diverse neuronal populations. This control necessary cue detection, learning, memory, disproportionately disrupted neurodegenerative diseases associated cognitive decline. Intriguingly, signaling acts locally within sculpt output neurons. To determine local impacts pathways that participate regulation, sought define dynamics during motivated behavior learning. Towards this, utilized fiber photometry genetically encoded acetylcholine indicator GAChR2.0 temporal patterns olfactory-guided, behaviors We show reliably increased reward-seeking was strongly suppressed by reward delivery go/no-go, olfactory-cued discrimination task. The observed transient reduction tone mirrored suppression GABAergic activity. Together, these findings suggest changes rapidly reflect rewardseeking positive reinforcement impact

Language: Английский

Citations

1