Deploying Viruses against Phytobacteria: Potential Use of Phage Cocktails as a Multifaceted Approach to Combat Resistant Bacterial Plant Pathogens DOI Creative Commons
Tahir Farooq, Muhammad Dilshad Hussain, Muhammad Taimoor Shakeel

et al.

Viruses, Journal Year: 2022, Volume and Issue: 14(2), P. 171 - 171

Published: Jan. 18, 2022

Plants in nature are under the persistent intimidation of severe microbial diseases, threatening a sustainable food production system. Plant-bacterial pathogens major concern contemporary era, resulting reduced plant growth and productivity. Plant antibiotics chemical-based bactericides have been extensively used to evade bacterial diseases. To counteract this pressure, bacteria evolved an array resistance mechanisms, including innate adaptive immune systems. The emergence resistant detrimental consequences antimicrobial compounds on environment human health, accentuates development alternative disease evacuation strategy. phage cocktail therapy is multidimensional approach effectively employed for biocontrol diverse infections without affecting fauna flora. Phages engage set counter defense strategies undermine wide-ranging anti-phage mechanisms pathogens. Microbial ecology, evolution, dynamics interactions between plant-bacterial lead engineering robust therapeutics mitigation devastating phytobacterial In review, we highlight concrete fundamental determinants application cocktails their underlying mechanism, combating Additionally, provide recent advances use against phytobacteria

Language: Английский

Antibiotic resistance in Vibrio cholerae: Understanding the ecology of resistance genes and mechanisms DOI Creative Commons
Bhabatosh Das, Jyoti Verma, Pawan Kumar

et al.

Vaccine, Journal Year: 2019, Volume and Issue: 38, P. A83 - A92

Published: July 2, 2019

The unique genetic makeup and remarkable competency of Vibrio cholerae are the key factors that help cholera pathogen adapt rapidly to adverse environmental conditions resist detrimental effect antimicrobial agents. In last few decades, V. causes acute watery diarrhoeal disease has emerged as a notorious multidrug resistant (MDR) enteric pathogen. Although chromosomal mutations can contribute resistance (AMR), frequent acquisition extrachromosomal mobile elements (MGEs) from closely/distantly related bacterial species major players in drug resistance. Whole genome sequence analysis clinical strains revealed most recent isolates harbour integrating conjugative (ICEs), plasmids, superintegron, transposable insertion sequences, which carriers traits encoding function. Different genes identified antibiotic by facilitating one following three mechanisms; (i) reduced permeability or active efflux antibiotics, (ii) alteration targets introducing post-transcriptional/translational modifications (iii) hydrolysis chemical modification antibiotics. Here, we present an overview insights on emergence mechanisms AMR cholerae.

Language: Английский

Citations

176
Revisiting the rules of life for viruses of microorganisms DOI
Adrienne M. S. Correa, Cristina Howard‐Varona, Samantha R Coy

et al.

Nature Reviews Microbiology, Journal Year: 2021, Volume and Issue: 19(8), P. 501 - 513

Published: March 24, 2021

Language: Английский

Citations

137
Diverse and abundant phages exploit conjugative plasmids DOI Creative Commons
Natalia Quinones‐Olvera, Siân V. Owen, Lucy M. McCully

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 12, 2024

Phages exert profound evolutionary pressure on bacteria by interacting with receptors the cell surface to initiate infection. While majority of phages use chromosomally encoded structures as receptors, plasmid-dependent exploit plasmid-encoded conjugation proteins, making their host range dependent horizontal transfer plasmid. Despite unique biology and biotechnological significance, only a small number have been characterized. Here we systematically search for new targeting IncP IncF plasmids using targeted discovery platform, find that they are common abundant in wastewater, largely unexplored terms genetic diversity. Plasmid-dependent enriched non-canonical types phages, all but one 65 isolated were non-tailed, members lipid-containing tectiviruses, ssDNA filamentous or ssRNA phages. We show tectiviruses exhibit differences which is associated variation phage holin protein. relatively high abundance missed metaviromic analyses, underscoring continued importance culture-based discovery. Finally, identify tailed plasmid, related structural genes orthogonal type 4 pilus receptor, highlighting evolutionarily promiscuous these distinct contractile multiple groups Taken together, results indicate play an under-appreciated role constraining gene via conjugative plasmids.

Language: Английский

Citations

19
Pf Bacteriophage and Their Impact on Pseudomonas Virulence, Mammalian Immunity, and Chronic Infections DOI Creative Commons
Patrick R. Secor, Elizabeth B. Burgener,

Margie Kinnersley

et al.

Frontiers in Immunology, Journal Year: 2020, Volume and Issue: 11

Published: Feb. 21, 2020

Pf bacteriophage are temperate phages that infect the bacterium

Language: Английский

Citations

94
Black box of phage–bacterium interactions: exploring alternative phage infection strategies DOI Creative Commons
Sari Mäntynen, Elina Laanto, Hanna M. Oksanen

et al.

Open Biology, Journal Year: 2021, Volume and Issue: 11(9)

Published: Sept. 1, 2021

The canonical lytic–lysogenic binary has been challenged in recent years, as more evidence emerged on alternative bacteriophage infection strategies. These modes are little studied, and yet they appear to be abundant ubiquitous nature than previously recognized, can play a significant role the ecology evolution of their bacterial hosts. In this review, we discuss extent, causes consequences phage lifestyles, clarify conceptual terminological confusion facilitate research progress. We propose distinct definitions for terms ‘pseudolysogeny’ ‘productive or non-productive chronic infection’, distinguish them from carrier state life cycle, which describes population-level phenomenon. Our review also finds that phages may change response environmental conditions physiological host cell. outline known molecular mechanisms underlying phage–host interactions, including specific genetic pathways considerable biotechnological potential. Moreover, potential implications lifestyles microbial biology ecosystem functioning, well applied topics such therapy.

Language: Английский

Citations

93
Bacteriophage and Bacterial Susceptibility, Resistance, and Tolerance to Antibiotics DOI Creative Commons
Qingquan Chen, Tejas Dharmaraj, Pamela C. Cai

et al.

Pharmaceutics, Journal Year: 2022, Volume and Issue: 14(7), P. 1425 - 1425

Published: July 7, 2022

Bacteriophages, viruses that infect and replicate within bacteria, impact bacterial responses to antibiotics in complex ways. Recent studies using lytic bacteriophages treat infections (phage therapy) demonstrate phages can promote susceptibility chemical phage/antibiotic synergy is possible. However, both lysogenic contribute antimicrobial resistance. In particular, some mediate the horizontal transfer of antibiotic resistance genes between bacteria via transduction other mechanisms. addition, chronic infection filamentous tolerance, ability persist face antibiotics. serve as structural elements biofilms prevent penetration Over time, these contributions tolerance favor selection clones. Here, we review recent insights into bacteriophage susceptibility, resistance, tolerance. We discuss mechanisms involved effects address their on fitness.

Language: Английский

Citations

55
Phage delivered CRISPR-Cas system to combat multidrug-resistant pathogens in gut microbiome DOI Creative Commons

Arijit Nath,

Rahul Bhattacharjee, Aditya Nandi

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2022, Volume and Issue: 151, P. 113122 - 113122

Published: May 17, 2022

The Host-microbiome interactions that exist inside the gut microbiota operate in a synergistic and abnormal manner. Additionally, normal homeostasis functioning of are frequently disrupted by intervention Multi-Drug Resistant (MDR) pathogens. CRISPR-Cas (CRISPR-associated protein with clustered regularly interspersed short palindromic repeats) recognized as prokaryotic immune system has emerged an effective genome-editing tool to edit delete specific microbial genes for expulsion bacteria through bactericidal action. In this review, we demonstrate many systems against anti-microbial resistance multiple pathogens, which infiltrate gastrointestinal tract. Moreover, discuss advancement development phage-delivered killing MDR pathogen. We also combinatorial approach use bacteriophage delivery gene targeting pathogenic community microbiome resensitize drug sensitivity. Finally, engineered phage plausible potential option improvement efficacy system.

Language: Английский

Citations

48
Engineered M13 phage as a novel therapeutic bionanomaterial for clinical applications: From tissue regeneration to cancer therapy DOI Creative Commons
Cheng Chang,

Wennan Guo,

Xinbo Yu

et al.

Materials Today Bio, Journal Year: 2023, Volume and Issue: 20, P. 100612 - 100612

Published: March 24, 2023

Bacteriophages (phages) are nanostructured viruses with highly selective antibacterial properties that have gained attention beyond eliminating bacteria. Specifically, M13 phages filamentous recently been studied in various aspects of nanomedicine due to their biological advantages and more compliant engineering capabilities over other phages. Having nanofiber-like morphology, can reach varied target sites self-assemble into multidimensional scaffolds a relatively safe stable way. In addition, genetic modification the coat proteins enables specific display peptides antibodies on phages, allowing for precise individualized medicine. also subjected novel approaches, including phage-based bionanomaterial phage-directed nanomaterial combinations enhance view these features, researchers able utilize therapeutic applications such as drug delivery, biodetection, tissue regeneration, targeted cancer therapy. particular, utilized precisely mimicking natural environment order overcome shortage organ donors. Hence, this review, we address recent studies advances using field agents based upon characteristics biomolecules displayed. This paper emphasizes approach enhances phage's capabilities. Current limitations future approaches discussed provide insight further progress clinical applications.

Language: Английский

Citations

36
Engineered Phage-Based Cancer Vaccines: Current Advances and Future Directions DOI Creative Commons

Murali Ragothaman,

So Young Yoo

Vaccines, Journal Year: 2023, Volume and Issue: 11(5), P. 919 - 919

Published: April 29, 2023

Bacteriophages have emerged as versatile tools in the field of bioengineering, with enormous potential tissue engineering, vaccine development, and immunotherapy. The genetic makeup phages can be harnessed for development novel DNA vaccines antigen display systems, they provide a highly organized repetitive presentation antigens to immune cells. opened new possibilities targeting specific molecular determinants cancer Phages used anticancer agents carriers imaging molecules therapeutics. In this review, we explored role bacteriophages bacteriophage engineering targeted therapy. question how engineered interact biological immunological systems is emphasized comprehend underlying mechanism phage use effectiveness technology identifying high-affinity ligands substrates, such cells tumor-associated molecules, emerging its effective treatments are discussed. We also highlight usage clinical trials well related patents. This review provides insight into phage-based vaccines.

Language: Английский

Citations

25
Emerging applications of phage therapy and fecal virome transplantation for treatment of Clostridioides difficile infection: challenges and perspectives DOI Creative Commons
Hamideh Raeisi, Maryam Noori, Masoumeh Azimirad

et al.

Gut Pathogens, Journal Year: 2023, Volume and Issue: 15(1)

Published: May 9, 2023

Abstract Clostridioides difficile , which causes life-threatening diarrheal disease, is considered an urgent threat to healthcare setting worldwide. The current standards of care solely rely on conventional antibiotic treatment, however, there a risk promoting recurrent C. infection (rCDI) because the emergence antibiotic-resistant strains. Globally, alarming spread strains has resulted in quest for alternative therapeutics. use fecal microbiota transplantation (FMT), involves direct infusion suspension from healthy donor into diseased recipient, been approved as highly efficient therapeutic option patients with rCDI. Bacteriophages or phages are group viruses that can infect and destroy bacterial hosts, recognized dominant viral component human gut microbiome. Accumulating data demonstrated play vital role microbial balance Recently, phage therapy virome (FVT) have introduced promising alternatives treatment - related infections, particular drug-resistant CDI. Herein, we review latest updates specific phages, phage-mediated treatments, highlight future prospects management

Language: Английский

Citations

23