Cellular stress management by caspases

Current Opinion in Cell Biology, Journal Year: 2024, Volume and Issue: 86, P. 102314 - 102314

Published: Jan. 11, 2024

Cellular stress plays a pivotal role in the onset of numerous human diseases. Consequently, removal dysfunctional cells, which undergo excessive stress-induced damage via various cell death pathways, including apoptosis, is essential for maintaining organ integrity and function. The evolutionarily conserved family cysteine-aspartic-proteases, known as caspases, has been key player orchestrating apoptosis. However, recent research unveiled capability these enzymes to govern fundamental cellular processes without triggering death. Remarkably, some non-lethal functions caspases may contribute restoring equilibrium stressed cells. This manuscript discusses how can function managers their potential impact on health disease. Additionally, it sheds light limitations caspase-based therapies, given our still incomplete understanding biology enzymes, particularly non-apoptotic contexts.

Language: Английский

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Novel 8-Methoxycoumarin-3-Carboxamides with potent anticancer activity against liver cancer via targeting caspase-3/7 and β-tubulin polymerization

BMC Chemistry, Journal Year: 2023, Volume and Issue: 17(1)

Published: Dec. 2, 2023

In the present study, we explored potential of coumarin-based compounds, known for their potent anticancer properties, by designing and synthesizing a novel category 8-methoxycoumarin-3-carboxamides. Our aim was to investigate antiproliferative activity against liver cancer cells. Toward this, developed versatile synthetic approach produce series 8-methoxycoumarin-3-carboxamide analogues with meticulous structural features. Assessment demonstrated significant inhibitory effects on growth HepG2 cells, widely studied cell line. Among screened compound 5 exhibited most among compounds (IC50 = 0.9 µM), outperforming drug staurosporine 8.4 while showing minimal impact normal The flow cytometric analysis revealed that induces cycle arrest during G1/S phase triggers apoptosis in cells increasing percentage arrested G2/M pre-G1 phases. Annexin V-FITC/PI screening further supported induction without necrosis. Further, ability activate caspase3/7 protein substantially inhibited β-tubulin polymerization Finally, molecular modelling affirmed high binding affinity toward active cavity protein, suggesting its mechanistic involvement. Collectively, our findings highlight therapeutic presented class coumarin analogues, especially 5, as promising candidates development effective anti-hepatocellular carcinoma agents.

Language: Английский

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Juvenile Hormone as a contributing factor in establishing midgut microbiota for fecundity and fitness enhancement in adult female Aedes aegypti

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: June 5, 2024

Abstract Understanding the factors influencing mosquitoes’ fecundity and longevity is important for designing better more sustainable vector control strategies, as these parameters can impact their vectorial capacity. Here, we address how mating affects midgut growth in Aedes aegypti , what role Juvenile Hormone (JH) plays this process, it impacts mosquito’s immune response microbiota. Our findings reveal that JH induce growth. Additionally, establishment of a native bacterial population due to JH-dependent suppression has reproductive outcomes. Specific downregulation AMPs with an increase bacteria abundance gut results increased egg counts longer lifespans. Overall, provide evidence cross-talk between response, epithelial tissue, cell cycle regulation, mechanisms governing trade-offs nutrition, immunity, reproduction at cellular level mosquito gut.

Language: Английский

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A near death experience: The secret stem cell life of caspase-3

Seminars in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 171, P. 103617 - 103617

Published: May 8, 2025

Language: Английский

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Exploring caspase-dependent non-lethal cellular processes using Drosophila

Frontiers in Cell Death, Journal Year: 2024, Volume and Issue: 3

Published: Oct. 21, 2024

Caspases are cysteine aspartic acid proteases conserved in animals that not only execute apoptosis, but also regulate diverse cellular processes independent of which termed caspase-dependent non-lethal (CDPs). Owing to its strong genetics detect and manipulate caspase activity cells interest vivo , Drosophila melanogaster serves as an excellent model organism for analyzing CDPs. This is further supported by the fact apoptotic signaling, well CDPs their mechanisms, are, part, other animals. Here, we present a review guide researchers studying using . In this review, provide overview current understanding regulates activation available genetic tools characteristics detecting manipulating so can choose appropriate own experimental settings. We introduce identified including brief description discovery characterization processes. describe underlying molecular mechanisms several well-characterized CDPs, regulatory enable activation. Finally, use proximity labeling techniques, especially TurboID, facilitates analysis mechanisms. Because caspases various functions, no longer considered point return cell death. Understanding will advance our states living dying cells, along with intermediate states.

Language: Английский

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Non‐apoptotic caspase activation ensures the homeostasis of ovarian somatic stem cells

EMBO Reports, Journal Year: 2023, Volume and Issue: 24(6)

Published: April 11, 2023

Current evidence has associated caspase activation with the regulation of basic cellular functions without causing apoptosis. Malfunction non-apoptotic activities may contribute to specific neurological disorders, metabolic diseases, autoimmune conditions and cancers. However, our understanding remains limited. Here, we show that prevents intracellular accumulation Patched receptor in autophagosomes subsequent Patched-dependent induction autophagy Drosophila follicular stem cells. These events ultimately sustain Hedgehog signalling physiological properties ovarian somatic cells their progeny under moderate thermal stress. Importantly, key findings are partially conserved human origin. observations attribute caspases a pro-survival role certain conditions.

Language: Английский

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Recent advances in fluorescence biosensor for caspase detecting and imaging in biomedical applications

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 168, P. 117337 - 117337

Published: Sept. 30, 2023

Language: Английский

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Something new under the sun: caspases promote cell survival under moderate heat stress

EMBO Reports, Journal Year: 2023, Volume and Issue: 24(6)

Published: May 11, 2023

Language: Английский

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