Genome-wide association in Drosophila identifies a role for Piezo and Proc-R in sleep latency

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 2, 2024

Abstract Sleep latency, the amount of time that it takes an individual to fall asleep, is a key indicator sleep need. latency varies considerably both among and within species heritable, but lacks comprehensive description its underlying genetic network. Here we conduct genome-wide association study latency. Using previously collected activity data on wild-derived population flies, calculate confirming significant, heritable variation for this complex trait. We identify 520 polymorphisms in 248 genes contributing variability Tests mutations 23 candidate additional putative pan-neuronal knockdown 9 them implicated CG44153 , Piezo Proc-R Rbp6 Two large-effect were further confirmed via rescue. This work greatly enhances our understanding factors influence

Language: Английский

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High-throughput and genome-scale targeted mutagenesis using CRISPR in a nonmodel multicellular organism,Bombyx mori

Genome Research, Journal Year: 2024, Volume and Issue: 34(1), P. 134 - 144

Published: Jan. 1, 2024

Large-scale genetic mutant libraries are powerful approaches to interrogating genotype–phenotype correlations and identifying genes responsible for certain environmental stimuli, both of which the central goal life science study. We produced first large-scale CRISPR-Cas9-induced library in a nonmodel multicellular organism, Bombyx mori . developed piggyBac -delivered binary genome editing strategy, can simultaneously meet requirements mixed microinjection, efficient multipurpose operation, preservation growth-defect lines. constructed single-guide RNA (sgRNA) plasmid containing 92,917 sgRNAs targeting promoters exons 14,645 protein-coding genes, established 1726 transgenic sgRNA lines following microinjection 66,650 embryos, generated 300 with diverse phenotypic changes. Phenomic characterization identified large set visual or economically valuable trait Next, we performed pooled context-specific positive screens tolerance pollutant cadmium exposure, KWMTBOMO12902 as strong candidate gene breeding applications sericulture industry. Collectively, our results provide novel versatile approach functional B. genomics, well resource potential key improving various economic traits. This study also shows effectiveness, practicality, convenience other organisms.

Language: Английский

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Future avenues inDrosophilamushroom body research

Learning & Memory, Journal Year: 2024, Volume and Issue: 31(5), P. a053863 - a053863

Published: May 1, 2024

How does the brain translate sensory information into complex behaviors? With relatively small neuronal numbers, readable behavioral outputs, and an unparalleled genetic toolkit, Drosophila mushroom body (MB) offers excellent model to address this question in context of associative learning memory. Recent technological breakthroughs, such as freshly completed full-brain connectome, multiomics approaches, CRISPR-mediated gene editing, machine techniques, led major advancements our understanding MB circuit at molecular, structural, physiological, functional levels. Despite significant progress individual areas, field still faces fundamental challenge resolving how these different levels combine interact ultimately control behavior fly. In review, we discuss various aspects research, with a focus on current knowledge gaps, outlook future methodological developments required reach overall view neurobiological basis

Language: Английский

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Rosy Beginnings: Studying Peroxisomes in Drosophila

Frontiers in Cell and Developmental Biology, Journal Year: 2020, Volume and Issue: 8

Published: Aug. 25, 2020

Research using the fruit fly Drosophila melanogaster has traditionally focused on understanding how mutations affecting gene regulation or function affect processes linked to animal development. Accordingly, flies have become an essential foundation of modern medical research through repeated contributions our fundamental their homologs human genes function. Peroxisomes are organelles that metabolize lipids and reactive oxygen species like peroxides. However, despite clear linkage in peroxisomes developmental defects, for many years models were conspicuously absent from study peroxisomes. Now, few early studies linking Rosy eye colour phenotype been joined by a growing body establishing novel roles during development specific tissues cell types. Similarly, unique properties cultured Schneider 2 cells advanced move cytoskeleton. Here, we profile those past more recent started link effects peroxisome dysfunction organ highlight utility as model peroxisomal diseases. We also identify key differences proliferation compared yeast mammals. Finally, discuss future system including new techniques should support identification additional tissue

Language: Английский

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Upgraded CRISPR/Cas9 tools for tissue-specific mutagenesis in Drosophila

Proceedings of the National Academy of Sciences, Journal Year: 2021, Volume and Issue: 118(14)

Published: March 29, 2021

CRISPR/Cas9 has emerged as a powerful technology for tissue-specific mutagenesis. However, tools currently available in

Language: Английский

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