Recruitment of Peroxin14 to lipid droplets affects triglyceride storage in Drosophila DOI Creative Commons

Matthew Anderson‐Baron,

Kazuki Ueda,

Julie Haskins

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: July 3, 2021

Abstract The activity of multiple organelles must be coordinated to ensure cellular lipid homeostasis. This includes the peroxisomes which metabolise certain lipids and droplets act as neutral storage centres. Direct organellar contact between has been observed, interaction proteins associated with membranes these shown, but functional role interactions is not clear. In Drosophila cells, we identified a novel localization subset three transmembrane Peroxin (Peroxin3, Peroxin13, Peroxin14), normally required for peroxisome biogenesis, newly formed droplets. event was linked significant changes in size or number, nor recruitment other mature observed. presence at influences their function relative levels Peroxin14 droplet surface directly affected regulatory perilipin lipases corresponding effects on triglyceride storage. Summary statement Interactions thought help coordinate management lipids. are biogenesis. A spectrum triacylglyceride seen when each 12 conserved Peroxins knocked down fat body knockdown having largest effect. When S2 cells were cultured excess oleic acid, Peroxin3, Peroxin14, localized independently markers. altered lipase proteins.

Language: Английский

Combined effects of a pharmaceutical pollutant, gemfibrozil, and abiotic stressors (warming and air exposure) on cellular stress responses of the blue mussels Mytilus edulis DOI Creative Commons
Halina Falfushynska, Eugene P. Sokolov, Inna M. Sokolova

et al.

Aquatic Toxicology, Journal Year: 2025, Volume and Issue: 279, P. 107233 - 107233

Published: Jan. 1, 2025

Language: Английский

Citations

0
Circadian clock-independent ultradian rhythms in lipid metabolism in the Drosophila fat body DOI Creative Commons

Blanca Lago Solis,

Rafael Koch, Emi Nagoshi

et al.

Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 110245 - 110245

Published: May 1, 2025

The role of circadian clocks in regulating metabolic processes is well known; however, their impact on states across species and life stages remains largely unexplored. This study investigates the relationship between rhythms regulation Drosophila larval fat body, a hub analogous to mammalian liver adipose tissue. Surprisingly, body period null mutants, which lack functional clock all tissues, exhibited 12-hour gene expression, particularly those involved peroxisome function, lipid metabolism, oxidative stress response. These transcriptomic were aligned with oscillations biogenesis activity, reactive oxygen levels, peroxidation. Furthermore, mutants storage, ultimately leading net reduction levels. Collectively, our results identify clock-independent ultradian metabolism that are essential for survival development.

Language: Английский

Citations

0
New insights into the functions of ACBD4/5-like proteins using a combined phylogenetic and experimental approach across model organisms DOI Creative Commons
Suzan Kors, Martin Schuster, Daniel C. Maddison

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Journal Year: 2024, Volume and Issue: 1871(8), P. 119843 - 119843

Published: Sept. 11, 2024

Language: Английский

Citations

3
Ergothioneine exhibits longevity-extension effect in Drosophila melanogaster via regulation of cholinergic neurotransmission, tyrosine metabolism, and fatty acid oxidation DOI
Hong-Yu Pan, Zhiwei Ye, Qianwang Zheng

et al.

Food & Function, Journal Year: 2021, Volume and Issue: 13(1), P. 227 - 241

Published: Nov. 20, 2021

Postulated mechanisms of lifespan extension by EGT: preservation CNS via the coordination cholinergic neurotransmission, tyrosine metabolism, and peroxisomal proteins, regulated autophagic activity, mitochondrial function.

Language: Английский

Citations

19
Insights Into the Peroxisomal Protein Inventory of Zebrafish DOI Creative Commons
Maki Kamoshita,

Rechal Kumar,

Marco Anteghini

et al.

Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 13

Published: Feb. 28, 2022

Peroxisomes are ubiquitous, oxidative subcellular organelles with important functions in cellular lipid metabolism and redox homeostasis. Loss of peroxisomal causes severe disorders developmental neurological abnormalities. Zebrafish emerging as an attractive vertebrate model to study well metabolism. Here, we combined bioinformatics analyses molecular cell biology reveal the first comprehensive inventory Danio rerio proteins, which systematically compared those human peroxisomes. Through analysis all PTS1-carrying demonstrate that D. lacks two well-known mammalian proteins (BAAT ZADH2/PTGR3), but possesses a putative malate synthase (Mlsl) verified differences presence purine degrading enzymes. Furthermore, revealed novel candidate , whose function localisation is discussed. Our findings confirm suitability zebrafish for peroxisome research open possibilities humans.

Language: Английский

Citations

14
Functional-metabolic coupling in distinct renal cell types coordinates organ-wide physiology and delays premature ageing DOI Creative Commons
Jack Holcombe, Helen Weavers

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Dec. 18, 2023

Precise coupling between cellular physiology and metabolism is emerging as a vital relationship underpinning tissue health longevity. Nevertheless, functional-metabolic within heterogenous microenvironments in vivo remains poorly understood due to complexity metabolic plasticity. Here, we establish the Drosophila renal system paradigm for linking mechanistic analysis of metabolism, at single-cell resolution, organ-wide physiology. Kidneys are amongst most energetically-demanding organs, yet exactly how individual cell types fine-tune meet their diverse, unique physiologies over life-course unclear. Integrating live-imaging metabolite organelle dynamics with spatio-temporal genetic perturbation intact functional tissue, uncover distinct signatures essential support healthy ageing. Cell type-specific programming glucose handling, PPP-mediated glutathione regeneration FA β-oxidation via dynamic lipid-peroxisomal networks, downstream differential ERR receptor activity, precisely match energetic demands whilst limiting damage premature senescence; however, dramatic dysregulation may underlie age-related dysfunction.

Language: Английский

Citations

8
Dysfunctional peroxisomal lipid metabolisms and their ocular manifestations DOI Creative Commons
Chuck T. Chen,

Zhuo Shao,

Zhongjie Fu

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: Sept. 7, 2022

Retina is rich in lipids and dyslipidemia causes retinal dysfunction eye diseases. In retina, are not only important membrane component cells organelles but also fuel substrates for energy production. However, our current knowledge of lipid processing the retina very limited. Peroxisomes play a critical role homeostasis genetic disorders with peroxisomal have different types ocular complications. this review, we focus on peroxisomes metabolism, including degradation detoxification very-long-chain fatty acids, branched-chain dicarboxylic reactive oxygen/nitrogen species, glyoxylate, amino as well biosynthesis docosahexaenoic acid, plasmalogen bile acids. We discuss potential contributions pathways to health summarize reported cases symptoms patients disorders, corresponding each disrupted pathway. review cross-talk between other such lysosomes, endoplasmic reticulum mitochondria.

Language: Английский

Citations

11
Peroxisomal ERK mediates Akh/glucagon action and glycemic control DOI Creative Commons
Jiaying Li,

Peixuan Dang,

Zhen Li

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 42(10), P. 113200 - 113200

Published: Oct. 1, 2023

The enhanced response of glucagon and its Drosophila homolog, adipokinetic hormone (Akh), leads to high-caloric-diet-induced hyperglycemia across species. While previous studies have characterized regulatory components transducing linear Akh signaling promoting carbohydrate production, the spatial elucidation action at organelle level still remains largely unclear. In this study, we find that phosphorylates extracellular signal-regulated kinase (ERK) translocates it peroxisome via calcium/calmodulin-dependent protein II (CaMKII) cascade increase production in fat body, leading hyperglycemia. mechanisms include ERK mediates body peroxisomal conversion amino acids into carbohydrates for gluconeogenesis Akh. Importantly, receptor (AkhR) or deficiency, importin-associated retention from peroxisome, inactivation sufficiently alleviates high-sugar-diet-induced We also observe mammalian glucagon-induced hepatic translocation diabetic subjects. Therefore, our results conclude Akh/glucagon-peroxisomal-ERK axis is a key regulator glycemic control.

Language: Английский

Citations

6
Recruitment of Peroxin 14 to lipid droplets affects lipid storage in Drosophila DOI Open Access

Kazuki Ueda,

Matthew Anderson‐Baron,

Julie Haskins

et al.

Journal of Cell Science, Journal Year: 2022, Volume and Issue: 135(7)

Published: March 11, 2022

Both peroxisomes and lipid droplets regulate cellular homeostasis. Direct inter-organellar contacts as well novel roles for proteins associated with peroxisome or occur when cells are induced to liberate fatty acids from droplets. We have shown a non-canonical role subset of peroxisome-assembly [Peroxin (Pex)] in this process Drosophila. Transmembrane Pex3, Pex13 Pex14 were observed surround newly formed Trafficking was enhanced by loss Pex19, which directs insertion transmembrane like into the bilayer membrane. Accumulation around did not induce changes size number, co-recruitment remaining Peroxins needed assemble observed. Increasing relative level surrounding affected recruitment Hsl lipase. Fat body-specific reduction these droplet-associated caused unique effect on larval fat body development their survival lipid-enriched minimal diets. This revealed heretofore unknown function Pex regulating storage. article has an First Person interview Kazuki Ueda, joint first author paper.

Language: Английский

Citations

8
Zebrafish model of human Zellweger syndrome reveals organ-specific accumulation of distinct fatty acid species and widespread gene expression changes DOI
Shigeo Takashima,

Shoko Takemoto,

Kayoko Toyoshi

et al.

Molecular Genetics and Metabolism, Journal Year: 2021, Volume and Issue: 133(3), P. 307 - 323

Published: May 8, 2021

Language: Английский

Citations

10