Dexamethasone Induced Osteocyte Apoptosis in Steroid‐Induced Femoral Head Osteonecrosis through ROS‐Mediated Oxidative Stress DOI Creative Commons
Xinglong Zhang,

Zhenhuan Yang,

Qian Xu

et al.

Orthopaedic Surgery, Journal Year: 2024, Volume and Issue: 16(3), P. 733 - 744

Published: Feb. 21, 2024

Objective Glucocorticoid (GC) overuse is strongly associated with steroid‐induced osteonecrosis of the femoral head (SINFH). However, underlying mechanism SINFH remains unclear. This study aims to investigate effect dexamethasone (Dex)‐induced oxidative stress on osteocyte apoptosis and mechanisms. Methods Ten patients 10 developmental dysplasia hips (DDH) were enrolled in our study. Sixty rats randomly assigned Control, Dex, Dex + N‐Acetyl‐L‐cysteine (NAC), Dibenziodolium chloride (DPI), NAC, DPI groups. Magnetic resonance imaging (MRI) was used examine edema rats. Histopathological staining performed assess osteonecrosis. Immunofluorescence TUNEL 8‐OHdG conducted evaluate damage. Immunohistochemical carried out detect expression NOX1, NOX2, NOX4. Viability MLO‐Y4 cells measured using CCK‐8 assay staining. stress. 2′,7′‐Dichlorodihydrofluorescein diacetate (DCFH‐DA) measure reactive oxygen species (ROS). The NOX4 analyzed by Western blotting. Multiple comparisons one‐way analysis variance (ANOVA). Results In rat model, hematoxylin–eosin (HE) revealed a significantly higher rate empty lacunae group than DDH group. indicated significant increase TUNEL‐positive 8‐OHdG‐positive compared demonstrated proteins patients. Moreover, immunohistochemical showed proportion NOX2‐positive Control vitro, inhibited viability induced apoptosis. After treatment, intracellular ROS level increased. treatment did not alter NOX vitro. Additionally, NAC generation partially alleviated vivo Conclusion demonstrates that GC promotes through ROS‐induced Furthermore, we found increased NOXs serves as an important source generation.

Language: Английский

Diosmin mitigates dexamethasone-induced osteoporosis in vivo: Role of Runx2, RANKL/OPG, and oxidative stress DOI Open Access
El‐Shaimaa A. Arafa,

Noran O. Elgendy,

Mai A. Elhemely

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 161, P. 114461 - 114461

Published: March 6, 2023

Secondary osteoporosis is commonly caused by long-term intake of glucocorticoids (GCs), such as dexamethasone (DEX). Diosmin, a natural substance with potent antioxidant and anti-inflammatory properties, clinically used for treating some vascular disorders. The current work targeted exploring the protective properties diosmin to counteract DEX-induced in vivo. Rats were administered DEX (7 mg/kg) once weekly 5 weeks, second week, vehicle or (50 100 mg/kg/day) next four weeks. Femur bone tissues collected processed histological biochemical examinations. study findings showed that alleviated impairments DEX. In addition, upregulated expression Runt-related transcription factor 2 (Runx2) phosphorylated protein kinase B (p-AKT) mRNA transcripts Wingless (Wnt) osteocalcin. Furthermore, counteracted rise levels receptor activator nuclear factor-kB ligand (RANKL) reduction osteoprotegerin (OPG), both induced Diosmin restored oxidant/antioxidant equilibrium exerted significant antiapoptotic activity. aforementioned effects more pronounced at dose level mg/kg. Collectively, has proven protect rats against augmenting osteoblast development while hindering osteoclast resorption. Our could be stand recommending supplementation patients chronically using GCs.

Language: Английский

Citations

21
Cuproptosis-a potential target for the treatment of osteoporosis DOI Creative Commons

Dinglin Li,

Zhonghua Gao,

Qian Li

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: May 5, 2023

Osteoporosis is an age-related disease of bone metabolism marked by reduced mineral density and impaired strength. The causes the bones to weaken break more easily. Osteoclasts participate in resorption than osteoblasts formation, disrupting homeostasis leading osteoporosis. Currently, drug therapy for osteoporosis includes calcium supplements, vitamin D, parathyroid hormone, estrogen, calcitonin, bisphosphates, other medications. These medications are effective treating but have side effects. Copper a necessary trace element human body, studies shown that it links development Cuproptosis recently proposed new type cell death. Copper-induced death regulates lipoylated components mediated via mitochondrial ferredoxin 1; is, copper binds directly tricarboxylic acid cycle, resulting protein accumulation subsequent loss iron-sulfur cluster proteins, proteotoxic stress eventually Therapeutic options tumor disorders include targeting intracellular toxicity cuproptosis. hypoxic environment metabolic pathway glycolysis provide energy cells can inhibit cuproptosis, which may promote survival proliferation various cells, including osteoblasts, osteoclasts, effector T macrophages, thereby mediating process. As result, our group tried explain relationship between role cuproptosis its essential regulatory genes, as well pathological mechanism effects on cells. This study intends investigate treatment approach clinical beneficial

Language: Английский

Citations

17
Association of the composite dietary antioxidant index with bone mineral density in the United States general population: data from NHANES 2005–2010 DOI

Huawei Han,

Daiwen Chen,

Xinzhe Wang

et al.

Journal of Bone and Mineral Metabolism, Journal Year: 2023, Volume and Issue: 41(5), P. 631 - 641

Published: June 8, 2023

Language: Английский

Citations

17
Polyphenols as potential preventers of osteoporosis: A comprehensive review on antioxidant and anti-inflammatory effects, molecular mechanisms, and signal pathways in bone metabolism DOI Open Access

Zhan Su,

Bin Yao,

Gang Liu

et al.

The Journal of Nutritional Biochemistry, Journal Year: 2023, Volume and Issue: 123, P. 109488 - 109488

Published: Oct. 20, 2023

Language: Английский

Citations

17
Dexamethasone Induced Osteocyte Apoptosis in Steroid‐Induced Femoral Head Osteonecrosis through ROS‐Mediated Oxidative Stress DOI Creative Commons
Xinglong Zhang,

Zhenhuan Yang,

Qian Xu

et al.

Orthopaedic Surgery, Journal Year: 2024, Volume and Issue: 16(3), P. 733 - 744

Published: Feb. 21, 2024

Objective Glucocorticoid (GC) overuse is strongly associated with steroid‐induced osteonecrosis of the femoral head (SINFH). However, underlying mechanism SINFH remains unclear. This study aims to investigate effect dexamethasone (Dex)‐induced oxidative stress on osteocyte apoptosis and mechanisms. Methods Ten patients 10 developmental dysplasia hips (DDH) were enrolled in our study. Sixty rats randomly assigned Control, Dex, Dex + N‐Acetyl‐L‐cysteine (NAC), Dibenziodolium chloride (DPI), NAC, DPI groups. Magnetic resonance imaging (MRI) was used examine edema rats. Histopathological staining performed assess osteonecrosis. Immunofluorescence TUNEL 8‐OHdG conducted evaluate damage. Immunohistochemical carried out detect expression NOX1, NOX2, NOX4. Viability MLO‐Y4 cells measured using CCK‐8 assay staining. stress. 2′,7′‐Dichlorodihydrofluorescein diacetate (DCFH‐DA) measure reactive oxygen species (ROS). The NOX4 analyzed by Western blotting. Multiple comparisons one‐way analysis variance (ANOVA). Results In rat model, hematoxylin–eosin (HE) revealed a significantly higher rate empty lacunae group than DDH group. indicated significant increase TUNEL‐positive 8‐OHdG‐positive compared demonstrated proteins patients. Moreover, immunohistochemical showed proportion NOX2‐positive Control vitro, inhibited viability induced apoptosis. After treatment, intracellular ROS level increased. treatment did not alter NOX vitro. Additionally, NAC generation partially alleviated vivo Conclusion demonstrates that GC promotes through ROS‐induced Furthermore, we found increased NOXs serves as an important source generation.

Language: Английский

Citations

7