Antibacterial and Antibiofilm Activity of Eucalyptus camaldulensis Derived Fe3O4 Nano-particles against Foodborne Pathogens DOI Open Access

Afshan Zia,

Sadia Tahir,

Saba Saba

et al.

Lahore Garrison University Journal of Life Sciences, Journal Year: 2024, Volume and Issue: 8(1), P. 97 - 116

Published: March 15, 2024

Foodborne pathogens are zoonotic and multidrug resistant, which not only affecting economy but also accountable for public health burdens. The present study was aimed to evaluate the efficacy of medicinal plant i.e., Eucalyptus camaldulensis extract mediated Fe3O4 ZnO nanoparticles against 27 foodborne pathogenic strains, isolated from milk, meat, dry fruits vegetable samples collected Multan, Pakistan. In phytochemical screening, found contain numerous bioactive compounds including flavonoids, phenolic compounds, tannins, quinones, anthocyanins. NPs synthesized displayed highest antibacterial activity with zones inhibition 12-13mm pathogens. were have anti-inflammatory potential recorded percentage 67% at 40 μg/ml. demonstrated antibiofilm after 120 hours incubation. For DDPH antioxidant assay, by their absorbance 1.43. Therefore, proved as an effective eco-friendly approach combat resistance in bacterial infections throughcharacteristic antibacterial, properties.

Language: Английский

Upgrading the antibacterial and antibiofilm potential of nanoruthenium via encapsulation by thiazolium ionic liquids-functionalized chitosan film DOI
Dalal Nasser Binjawhar, Mohammad Y. Alfaifi, Mohamed A. El Hamd

et al.

European Polymer Journal, Journal Year: 2024, Volume and Issue: 207, P. 112822 - 112822

Published: Feb. 6, 2024

Language: Английский

Citations

4
Genetically engineered phages and engineered phage-derived enzymes to destroy biofilms of antibiotics resistance bacteria DOI Creative Commons

Fatemeh Eghbalpoor,

Mahdieh Gorji,

Maryam Zamani Alavigeh

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(15), P. e35666 - e35666

Published: Aug. 1, 2024

Language: Английский

Citations

4
Dielectric barrier discharge air plasma as a comprehensive disinfection approach to E. coli and S. aureus biofilm management DOI Creative Commons
Viktor Udachin, Sebastian Dahle, Rok Fink

et al.

Physica Scripta, Journal Year: 2025, Volume and Issue: 100(2), P. 025015 - 025015

Published: Jan. 2, 2025

Abstract This study aimed to assess the potential of Dielectric Barrier Discharge (DBD) cold atmospheric air plasma for E. coli and S. aureus biofilm management. The bacterial biofilms were grown on stainless-steel coupons treated with 1 5 min. results indicate reduction up 4.3 log CFU ml −1 3.3 , respectively. optical emission spectroscopy (OES) shows a possible presence OH O species as well abundance N 2 + features in plasma. Furthermore, Fourier Transform Infrared Spectroscopy (FTIR) revealed changes wavelengths that are specific carbohydrates, lipids proteins when effect was also confirmed by x-ray photoelectron (XPS) carbon decreased after treatment whereas phosphorus potassium increased due release from destructed cell wall. confocal laser scanning microscopy (CLSM) removing surface, where this most predominant min treatment. All all, our demonstrates DBD can effectively reduce viability reactive nitrogen oxygen within phase. Moreover, applied resulted successful eradication surface coupons.

Language: Английский

Citations

0
Echinacoside, a promising sortase A inhibitor, combined with vancomycin against murine models of MRSA-induced pneumonia DOI
Tao Jiang, Dai Yuan, Rong Wang

et al.

Medical Microbiology and Immunology, Journal Year: 2023, Volume and Issue: 212(6), P. 421 - 435

Published: Oct. 5, 2023

Language: Английский

Citations

10
Enhancing Surgical Outcomes: A Critical Review of Antibiotic Prophylaxis in Orthopedic Surgery DOI Open Access

Gaurav K Upadhyyaya,

Sachchidanand Tewari

Cureus, Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 27, 2023

The postoperative burden remains significant due to the possibility of prolonged hospitalization, escalated healthcare costs, and patient distress caused by postorthopedic surgical site infections (SSIs). Orthopedic surgery is likewise faced with a challenge posed these conditions. A positive association has been observed between presence SSIs heightened susceptibility adverse health outcomes, along elevated rates morbidity mortality. Systemic antibiotic prophylaxis (SAP) reduces risk acquiring an SSI. Closed fractures, open arthroplasty, percutaneous fixation each possess distinct attributes that impact data antimicrobial therapy. When implementing SAP, it crucial strike delicate equilibrium maintaining effective stewardship protocols preventing occurrence SSIs. This practice effectively prevents both incidence negative consequences emergence resistance. objective this study was examine existing literature on use in orthopedic explore potential associated inappropriate administration antibiotics.

Language: Английский

Citations

9
Antimicrobial and Antibiofilm Potential of Flourensia retinophylla against Staphylococcus aureus DOI Creative Commons
Minerva Edith Beltrán-Martínez, Melvin R. Tapia-Rodríguez, J. Fernando Ayala‐Zavala

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(12), P. 1671 - 1671

Published: June 17, 2024

Staphylococcus aureus is a Gram-positive bacteria with the greatest impact in clinical area, due to high rate of infections and deaths reaching every year. A previous scenario associated bacteria’s ability develop resistance against conventional antibiotic therapies as well biofilm formation. The above situation exhibits necessity reach new effective strategies this pathogen. Flourensia retinophylla medicinal plant commonly used for bacterial treatments has demonstrated antimicrobial effect, although its effect S. biofilms not been investigated. purpose work was analyze antibiofilm potential F. aureus. determined using an ethanolic extract retinophylla. surface charge membrane, K+ leakage on motility were determined. prevent remove analyzed terms biomass, metabolic activity viability. results showed that presents inhibitory (MIC: 250 µg/mL) bactericidal (MBC: 500 MIC increased by 1.4 times concentration extracellular medium 60%. inhibited process 100%, 61% 40% after 24, 48 72 h, respectively. prevented formation more than 80% biomass production activity. An at 10 × reduced 82% viability ≈50% preformed biofilms. suggest affects areus membrane removal. This could set precedent use alternative disinfectant control caused In addition, shrub be considered carrying out purification order identify compounds responsible effect.

Language: Английский

Citations

3
Photodynamic Therapy of LD4-Photosensitizer Attenuates the Acute Pneumonia Induced by Klebsiella pneumoniae DOI Open Access
Tao Zhuo, Xin Li, Hongzhi Yu

et al.

ACS Pharmacology & Translational Science, Journal Year: 2024, Volume and Issue: 7(4), P. 1101 - 1113

Published: March 5, 2024

Klebsiella pneumoniae is a Gram-negative bacterium that induces acute lung injury (ALI) and inflammation in humans, necessitating immediate hospitalization treatment. At present, the clinical treatment largely dependent on hormones or antibiotics but associated with drawbacks posed by lack of eradication upon drug resistance. Therefore, there an urgent need for novel effective treatments. The current study investigated K. pneumonia-induced ALI using photosensitizer LD4 conjunction photodynamic therapy (PDT). water content lungs (corresponding to edema) rat model pneumonia induced was reduced LD4-PDT. counts leukocyte, lymphocyte, polymorphonuclear leukocyte blood were determined pneumonia, as concentrations inflammatory cytokines (estimated enzyme-linked immunosorbent assay). LD4-PDT prominently levels interleukin (IL)-6, IL-10, tumor necrosis factor-α, superoxide dismutase, immune cells. Results suggest considerably alleviates oxidative stress caused pneumonia. Furthermore, it could effectively improve survival rate ameliorate histological changes while protecting integrity pulmonary epithelial These results highlight potential application treating pneumoniae.

Language: Английский

Citations

2
Effects of honokiol combined with resveratrol on bacteria responsible for oral malodor and their biofilm DOI Creative Commons

Shiqian Zheng,

Rongrong Deng,

Gengjiu Huang

et al.

Journal of Oral Microbiology, Journal Year: 2024, Volume and Issue: 16(1)

Published: June 9, 2024

This study aimed to investigate the effect of honokiol combined with resveratrol on bacteria responsible for oral malodor and their biofilm.

Language: Английский

Citations

2
A global bibliometric and visualized analysis of bacterial biofilm eradication from 2012 to 2022 DOI Creative Commons
Tao Wang, Rui Zhang, Zhiling Chen

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 22, 2023

Background To deeply explore the dynamic trends, focal points and emerging topics of bacterial biofilm eradication field provide novel insights for prospective research endeavors, first global bibliometric visualized analysis was employed in this study. Methods The study meticulously curated articles reviews concentrating on from Web Science Core Collection (WoSCC) identified literature published 2012–2022 further analysis, performed to elucidate a clustering pattern domain with tools mainly including CiteSpace VOSviewer. Results 15,503 authors affiliated 2,397 institutions spanning 96 countries or regions contributed corpus 3,201 articles, containing 7,005 keywords. USA emerged as commanding vanguard exploring antibiofilm strategies displaying pioneering initiatives within sphere. Chinese Academy Sciences (CAS) most prolific source publications. Noteworthy among authors, Pandian Shunmugiah Karutha secured lead article contributions well co-citations while Deng Le his team is poised become dominant influence future. Despite that, extent collaborative engagement across different appeared fall short its potential. Frontiers Microbiology led discourse by publishing substantial body standing recurrently co-cited publication. influential domains encompassed “bacterial formation, “photodynamic therapy” “phage therapy.” Recent trends forefronts concentrate intensifying into facilitating shift multiple controlled lab settings animal studies real-world clinical environments. Conclusion Fundamentally, presents comprehensive scrutiny reveals that realm undergoing rapid evolution, even greater expansion anticipated times ahead. Subsequent scholars should emphasize augmentation efforts focus their energies topics, thus contributing break through current barriers transitioning “fundamental” stage “practical” application.

Language: Английский

Citations

5
CRISPRi-Mediated Gene Silencing in Biofilm Cycle and Quorum Sensing DOI
Asha Arora

Published: Jan. 1, 2024

Biofilm is an assembly of architecturally, bacterial mono-species with different functional attributes but similar genetical makeup. It generally formed when multicellularity induced to cope up the survival their population density at threshold level. BfC (biofilm cycle) undergoes four phases, namely, attachment on substratum (both biotic as well abiotic), nonmotile interactive growth phase simulated by QS (quorum sensing) factors, biosynthesis EPS (exopolymeric substances) matrix and its mediated maturation, fourth disassembly factors inducing detachment. These phases are regulated activation number genes (csrA, fliA, flhD, luxS, lsrK, lsrR, qseB, mqsR, pfs, qseC, motA etc) that governs virulence factors. bcsA operon, csgD, fimB, hha, pgaC, etc. reported be activated only during while quorum sensing achieved through autoinducers. N-acyl-homoserine lactones autoinducing peptides which form AHL system Gram-negative bacteria AIP Gram-positive bacteria, respectively, serve major signaling molecules in sensing. Prokaryotes possess multiple pathways repair endogenous DNA double-strand breaks via genomes deploying CRISPR-Cas menacing alien from varied sources. Therefore, CRISPR interference (CRISPRi) inhibits biofilm formation changing gene expression cassettes. In CRISPRi, small guide RNA activates inactive dCas9 proteins catalytically adhere corresponding promoter region turn silencing sterically hindering transcription initiation or elongation. CRISPRi strategy also goes sensor kinase reproducing phenotypic flaws surface motility. Decreased motility, swarming movements, lack adhesins, derangement assist decreasing pathogenicity.

Language: Английский

Citations

1