Hydrogels in Gene Delivery Techniques for Regenerative Medicine and Tissue Engineering DOI

Kexing Xu,

Qinmeng Zhang,

Danji Zhu

et al.

Macromolecular Bioscience, Journal Year: 2024, Volume and Issue: 24(6)

Published: Jan. 24, 2024

Hydrogels are 3D networks swollen with water. They biocompatible, strong, and moldable emerging as a promising biomedical material for regenerative medicine tissue engineering to deliver therapeutic genes. The excellent natural extracellular matrix simulation properties of hydrogels enable them be co-cultured cells or enhance the expression viral non-viral vectors. Its biocompatibility, high strength, degradation performance also make action process carriers in tissues more ideal, making it an ideal material. It has been shown that hydrogel-based gene delivery technologies have potential play therapy-relevant roles organs such bone, cartilage, nerve, skin, reproductive organs, liver animal experiments preclinical trials. This paper reviews recent articles on explains manufacture, applications, developmental timeline, limitations, future directions techniques.

Language: Английский

Precision Nanomedicine with Bio-Inspired Nanosystems: Recent Trends and Challenges in Mesenchymal Stem Cells Membrane-Coated Bioengineered Nanocarriers in Targeted Nanotherapeutics DOI Creative Commons
Mirza Salman Baig, Anas Ahmad, Rijawan Rajjak Pathan

et al.

Journal of Xenobiotics, Journal Year: 2024, Volume and Issue: 14(3), P. 827 - 872

Published: June 24, 2024

In the recent past, formulation and development of nanocarriers has been elaborated into broader fields opened various avenues in their preclinical clinical applications. particular, cellular membrane-based nanoformulations have formulated to surpass surmount limitations restrictions associated with naïve or free forms therapeutic compounds circumvent physicochemical immunological barriers including but not limited systemic barriers, microenvironmental roadblocks, other subcellular hinderances-which are quite heterogeneous throughout diseases patient cohorts. These drug delivery overcome through mesenchymal cells precision therapeutics, where these interventions led significant enhancements efficacies. However, still focuses on optimization paradigms a one-size-fits-all resolutions. As stem cell engineered highly diversified fashions, being optimized for delivering payloads more better personalized modes, entering arena as well nanomedicine. this Review, we included some advanced which designed utilized both non-personalized applicability can be employed improvements nanotherapeutics. present report, authors focused aspects advancements nanoparticle conceptions several roadblocks It suggested that well-informed designing will lead appreciable efficacy payload approaches also enable tailored customized designs MSC-based applications, finally amending outcomes.

Language: Английский

Citations

5
Re-establishing immune tolerance in multiple sclerosis: focusing on novel mechanisms of mesenchymal stem cell regulation of Th17/Treg balance DOI Creative Commons

Huiru Hu,

Hui Li, Ruoyu Li

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: July 15, 2024

The T-helper 17 (Th17) cell and regulatory T (Treg) axis plays a crucial role in the development of multiple sclerosis (MS), which is regarded as an immune imbalance between pro-inflammatory cytokines maintenance tolerance. Mesenchymal stem (MSC)-mediated therapies have received increasing attention MS research. In its animal model experimental autoimmune encephalomyelitis, MSC injection was shown to alter differentiation CD4

Language: Английский

Citations

5
Stem cell-derived exosome treatment for acute spinal cord injury: a systematic review and meta-analysis based on preclinical evidence DOI Creative Commons

Chunlin Mou,

Zhi Xia, Xiujuan Wang

et al.

Frontiers in Neurology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 24, 2025

Background The study aims were to systematically review and analyze preclinical research on the efficacy of exosomes derived from various mesenchymal stem cell sources (MSC-exos) for treatment spinal cord contusion injury (SCI) in small animal models. Methods We conducted a systematic search PubMed, Embase Google Scholar databases their inception through February 29, 2024, identify eligible English-language studies based predefined inclusion exclusion criteria. Two independent investigators performed literature screening, data extraction bias assessment. Results A total 235 rats used assess locomotor recovery at initial assessment, exhibited significant improvement hind limb movement those treated with exosomes, as indicated by statistically increase Basso-Beattie-Bresnahan (BBB) scores (MD: 1.26, 95% CI: 1.14–1.38, p < 0.01) compared controls. This trend persisted final assessment across 21 studies, pooled analysis confirming similar results 1.56, 1.43–1.68, 0.01). Funnel plot asymmetry BBB both baseline endpoint assessments, suggesting potential publication bias. Exosomes bone marrow, adipose tissue, umbilical or human placental MSCs. Meta-analysis showed no differences therapeutic among these MSC-exos time points. Conclusion demonstrated considerable promise improving motor function SCI-affected rats, marrow MSC-derived having particularly notable effectiveness.

Language: Английский

Citations

0
Injectable thermosensitive selenium-containing hydrogel as mesenchymal stem cell carrier to improve treatment efficiency in limb ischemia DOI Creative Commons
Xuan Tian, Xin Yan, Nan Zang

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 25, P. 100967 - 100967

Published: Jan. 21, 2024

Limb ischemia is a refractory disease characterized by persistent inflammation, insufficient angiogenesis, and tissue necrosis. Although mesenchymal stem cells (MSCs) have shown potential for treating limb ischemia, their therapeutic effects are limited low engraftment rates. Therefore, developing an optimal MSC delivery system that enhances cell viability imperative. Selenium, known its cytoprotective properties in various types, offers strategy to enhance effect of MSCs. In this study, we evaluated the selenium on MSCs, developed injectable thermosensitive selenium-containing hydrogel based PLGA-PEG-PLGA triblock copolymer, as carrier improve after engraftment. The biocompatibility, biodegradability, capabilities hydrogels were assessed. Furthermore, MSCs encapsulated within was using cellular animal experiments. Selenium protects from oxidative damage upregulating GPX4 through transcriptional mechanism. exhibited favorable antioxidant properties. It can be easily injected into target area liquid form at room temperature undergoes gelation body temperature, thereby preventing diffusion promoting cytoprotection effectively inhibited macrophage M1 polarization while M2 polarization, thus accelerating angiogenesis restoring blood perfusion ischemic limbs. This study demonstrated promising method delivery. By addressing challenge retention rate, which major obstacle application, improves ischemia.

Language: Английский

Citations

4
The sphingolipids change in exosomes from cancer patients and association between exosome release and sphingolipids level based on a pseudotargeted lipidomics method DOI

Kaige Yang,

Wenchang Fu,

Mengjiao Deng

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1305, P. 342527 - 342527

Published: March 25, 2024

Language: Английский

Citations

4
HWJMSC-EVs promote cartilage regeneration and repair via the ITGB1/TGF-β/Smad2/3 axis mediated by microfractures DOI Creative Commons

Zhian Chen,

Tianhua Zhou, Huan Luo

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: April 12, 2024

Abstract The current first-line treatment for repairing cartilage defects in clinical practice is the creation of microfractures (MF) to stimulate release mesenchymal stem cells (MSCs); however, this method has many limitations. Recent studies have found that MSC-derived extracellular vesicles (MSC-EVs) play an important role tissue regeneration. This study aimed verify whether MSC-EVs promote damage repair mediated by MFs and explore mechanisms. In vitro experiments showed human umbilical cord Wharton’s jelly (hWJMSC-EVs) promoted vitality chondrocytes proliferation differentiation ability bone marrow-derived MSCs. was mainly because hWJMSC-EVs carry integrin beta-1 (ITGB1), MSCs overexpress ITGB1 after absorbing EVs, thereby activating transforming growth factor-β/Smad2/3 axis. a rabbit knee joint model osteochondral defect repair, injection different concentrations into cavity concentration 50 µg/ml significantly improved formation transparent MF surgery. Extraction regenerated revealed changes ITGB1, factor-β, Smad2/3 were directly proportional cartilage. summary, Graphical abstract

Language: Английский

Citations

4
The role of exosomes derived from stem cells in nerve regeneration: A contribution to neurological repair DOI
Qiankun Zhang, Jiale Liu, Wei Wang

et al.

Experimental Neurology, Journal Year: 2024, Volume and Issue: 380, P. 114882 - 114882

Published: July 14, 2024

Language: Английский

Citations

4
Identification of Common Angiogenesis Marker Genes in Chronic Lung Diseases and Their Relationship with Immune Infiltration Based on Bioinformatics Approaches DOI Creative Commons
Lu Liu, Man Wang,

Shihuan Yu

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(2), P. 331 - 331

Published: Jan. 31, 2025

Objective: This study aims to explore the role of angiogenesis-related genes in chronic lung diseases (ILD and COPD) using bioinformatics methods, with goal identifying novel therapeutic targets slow disease progression prevent its deterioration into fibrosis or pulmonary artery hypertension. Methods: The research methods encompassed differential analysis, WGCNA (Weighted Gene Co-expression Network Analysis), multiple machine learning approaches screen for key genes. Set Enrichment Analysis (GSEA), Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) were utilized assess related biological functions pathways. Additionally, immune cell infiltration was analyzed evaluate status correlation between immunity. Results: COPD ILD are closely associated pathways angiogenesis, responses, others, both groups linked inflammation-related signaling established a disease-related gene set comprising 171 further screened out 21 angiogenesis. Ultimately, four genes—COL10A1, EDN1, MMP1, RRAS—were identified through methods. These angiogenesis processes, clustering analysis based on them can reflect different states variations infiltration. Conclusions: COL10A1, RRAS represent potential slowing preventing their deterioration. Furthermore, monocytes exhibited consistent patterns across control groups, as well among subgroups, suggesting significant development diseases.

Language: Английский

Citations

0
Comparing the therapeutic influence of bone marrow Mesenchymal stem cells versus its derived exosomes against diabetic hepatopathy in rats DOI

Rehab Mahmoud Kher-Eldin,

Yara S. Abouelela, Noha A. E. Yasin

et al.

Experimental Cell Research, Journal Year: 2025, Volume and Issue: unknown, P. 114436 - 114436

Published: March 1, 2025

Language: Английский

Citations

0
Bone marrow mesenchymal stem cell exosomes improve fracture union via remodeling metabolism in nonunion rat model DOI Creative Commons
Cheng Li, Ming Chen,

Lijun Guo

et al.

Journal of Orthopaedic Surgery and Research, Journal Year: 2025, Volume and Issue: 20(1)

Published: March 25, 2025

Nonunion of fractures is a major unsolved problem in clinical treatment and prognosis orthopedics. Bone marrow mesenchymal stem cell (BMSC) exosomes have been proven to be involved mediating tissue bone regeneration variety diseases. However, the role BMSC fracture nonunion unclear. were injected into rat model fracture, fracture-healing site was detected by micro-CT serum metabolites analyzed LC-MS/MS. The results showed that could successfully isolated from BMSCs cultured an exosome-free medium. Compared with group, exosome-treated rats healing obviously. PBS there 158 up-regulated differential abundance (DAMs) 79 down-regulated DAMs BMSC-exo group. enriched 'Th1 Th2 differentiation', 'ErbB signaling pathway', 'PPAR pathway' 'HIF-1 related function proliferation differentiation. DAMs-PE HIF-1 pathway metabolite promote healing. Our study reveals mechanism which BMSC-exosome improves process through metabolic reprogramming provides reference for nonunion.

Language: Английский

Citations

0