miR-550a-5p promotes the proliferation and migration of hepatocellular carcinoma by targeting GNE via the Wnt/β-catenin signaling pathway DOI Creative Commons
Chunming Li,

Wu Wu,

Zhu Zhu

et al.

Neoplasma, Journal Year: 2022, Volume and Issue: 69(06), P. 1359 - 1372

Published: Jan. 1, 2022

Liver cancer is one of the most common tumors with a high malignant degree in world. Its diagnosis and treatment are very difficult limited. More novel powerful DAT strategies urgently needed to break this situation. An increasing number studies have shown that microRNAs (miRNAs) could be used not only as biomarkers for prognosis hepatocellular carcinoma (HCC) but also important targets molecular targeted therapy. However, role miR-550a-5p HCC its specific mechanism remain unclear. Here we proposed verified hypothesis regulate progression was positively associated poor prognosis. We found decreased would inhibit proliferation migration cell lines (HCs) by performing relevant assays. Interestingly, knocking down GNE reverse above effect on HCs. Meanwhile, western blot results showed Wnt/β-catenin signaling pathway at least partly involved regulation miR-550a-5p. In addition, suppress growth vivo via xenograft tumor model assay. All all, draw conclusion miR-550a-5p/GNE axis functioned an promoting pathway.

Language: Английский

Novel PBMC LncRNA signatures as diagnostic biomarkers for colorectal cancer DOI
Zhaosheng Li,

Dongfeng Wang,

Wenjun Zhang

et al.

Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 253, P. 154985 - 154985

Published: Nov. 28, 2023

Language: Английский

Citations

9
Long Non-Coding RNAs in Kidney Injury: A Comprehensive Review DOI Creative Commons
Ramdas Bhat, Preeti Shanbhag

Journal of Prevention Diagnosis and Management of Human Diseases, Journal Year: 2024, Volume and Issue: 42, P. 39 - 52

Published: March 9, 2024

Kidney injury, a global health challenge, necessitates nuanced understanding of molecular intricacies for effective interventions. Long non-coding RNAs (lncRNAs), once dismissed as transcriptional noise, now emerge pivotal players in orchestrating renal health. Dysregulation specific lncRNAs like TUG1, MALAT1, H19, and NEAT1 provides signatures, distinguishing physiological states from pathological conditions. In acute kidney injury (AKI), TUG1 MALAT1 regulate apoptosis, inflammation, fibrosis. Chronic disease (CKD) involves H19 modulating cell proliferation apoptosis. Beyond diagnostics, actively shape fibrosis, positioning them master regulators the intricate ballet Recent strides research, coupled with cutting-edge genomics bioinformatics tools, highlight their roles therapeutic potential. Challenges interactions necessitate exploration promising avenues, including single-cell RNA sequencing artificial intelligence, paving way personalized interventions regenerative medicine diseases.

Language: Английский

Citations

1
Clinical validation of peripheral blood mononuclear cell DNA methylation markers for accurate early detection of hepatocellular carcinoma in Asian patients DOI Creative Commons
David Cheishvili,

Chifat Wong,

Mohammad Mahbubul Karim

et al.

Communications Medicine, Journal Year: 2024, Volume and Issue: 4(1)

Published: Oct. 30, 2024

Hepatocellular carcinoma (HCC), a leading cause of cancer-related deaths globally, poses significant challenges in early detection. Improved diagnostic accuracy can drastically influence patient outcomes, emphasizing the need for innovative, non-invasive biomarkers. This study utilized cohort 402 participants, including healthy controls, chronic hepatitis patients, and HCC patients from Bangladesh, to evaluate DNA methylation signatures peripheral blood mononuclear cells (PBMC). We performed targeted next-generation sequencing on selected genes previously identified assess their dynamics. The development M8 M4 scores was based these dynamics, using Receiver Operating Characteristic (ROC) analysis determine effectiveness detecting early-stage alongside existing markers such as epiLiver alpha-fetoprotein (AFP). Integration with AFP significantly enhances sensitivity HCC. M4+epiLiver score achieves 79.4% Stage A HCC, while combining increases 88.2–95.7% across all stages, indicating superior performance compared each marker used alone. Our confirms that gene profiles established substantially improves integrated approach holds promise advancing cancer diagnostics, potentially earlier treatment interventions improved survival rates high-risk patients. Cheishvili et al. investigate use detection hepatocellular an Asian cohort. demonstrates biomarkers accurately distinguish between controls different stages disease. Liver is one top causes death worldwide, finding it crucial successful treatment. research focuses simple test look specific changes signal liver cancer. tested this method diverse group people those already at high risk due infections. By new other tests, we were able detect more than by traditional methods could make easier less invasive find early, offering better chance effective hopeful prognosis risk.

Language: Английский

Citations

1
Multiomics characterization of fatty acid metabolism for the clinical management of hepatocellular carcinoma DOI Creative Commons
Xin Huang, Benzhe Su, Mengjun Li

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Dec. 18, 2023

Abstract Hepatocellular carcinoma (HCC) is a prevalent malignancy and there lack of effective biomarkers for HCC diagnosis. Living organisms are complex, different omics molecules interact with each other to implement various biological functions. Genomics metabolomics, which the top bottom systems biology, play an important role in clinical management. Fatty acid metabolism associated malignancy, prognosis, immune phenotype cancer, potential hallmark malignant tumors. In this study, genes metabolites related fatty were thoroughly investigated by dynamic network construction algorithm named EWS-DDA early diagnosis prognosis HCC. Three gene ratios eight metabolite identified as Further analysis using analysis, statistical document validation discovery sets suggested that selected had great prognostic value helped achieve Experimental results in-depth evaluation from viewpoints can facilitate further understanding pathological alterations characteristics, improving performance prognosis.

Language: Английский

Citations

3
Circ_0084615 promotes epithelial‐mesenchymal transition‐mediated tumor progression in hepatocellular carcinoma DOI Creative Commons
Yu Wu, Li Peng

Annals of Gastroenterological Surgery, Journal Year: 2024, Volume and Issue: 8(6), P. 1107 - 1117

Published: June 3, 2024

Abstract Aim CircRNAs have been identified as crucial regulators in tumorigenesis and progression. This study aimed to explore the biological role underlying mechanism of circ_0084615 hepatocellular carcinoma (HCC). Methods The expression RNAs was detected by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). effects silencing on malignant behaviors HCC cells were assessed CCK‐8, colony formation, wound healing, Transwell assays vitro tumor transplantation experiment vivo. proteins Western blotting. Dual‐luciferase reporter assay RNA‐binding protein immunoprecipitation performed circ_0084615. Results A significant upregulation observed tissues, positively correlated with TNM staging. Silencing impeded cell viability, migration, invasion, epithelial‐mesenchymal transition, xenograft growth. Mechanistically, could bind miR‐1200 eliminate its ability destroy actin‐like 6A (ACTL6A) mRNA, thereby increasing ACTL6A facilitating cells. Conclusions clarified oncogenic activity circ_0084615, providing potential diagnostic biomarker therapeutic target for inhibiting

Language: Английский

Citations

0
The combination of serum lncRNA PTTG3P and mRNA PTTG1 serves as a diagnostic and prognostic marker for hepatocellular carcinoma DOI Creative Commons
Shunwang Cao, Fei Zhong, Xueying Chen

et al.

Molecular Medicine Reports, Journal Year: 2024, Volume and Issue: 31(2)

Published: Nov. 29, 2024

Long noncoding RNA (lncRNA) PTTG3P has been demonstrated to participate in the development of hepatocellular carcinoma (HCC) by targeting mRNA PTTG1. The present study aimed investigate diagnostic efficacy serum lncRNA PTTG3P, PTTG1 and their combination for diagnosis prognosis HCC. A total 373 participants were enrolled study, including 73 patients with HCC, 100 chronic hepatitis B (CHB), liver cirrhosis (LC) healthy controls (HCs). expression levels RNAs quantified reverse transcription‑quantitative PCR. association between clinical characteristics was further analyzed. Receiver operating characteristic (ROC) curve area under (AUC) analyses performed estimate ability combinations other biomarkers results revealed that markedly increased HCC CHB compared HCs. Additionally, postoperative significantly lower than preoperative concentrations 36 paired Spearman's correlation coefficient analysis showed correlated aspartate transaminase (AST). ROC both had a significant predictive value AUC values alone 0.636 0.634, respectively. Furthermore, combining PTTG1, α‑fetoprotein (AFP), alanine aminotransferase (ALT), AST, γ‑glutamyl transpeptidase (GGT) alkaline phosphatase (ALP) value. best performance AFP, ALT, GGT ALP an 0.959, sensitivity 90.4% specificity 98.0%. In conclusion, AFP appeared be noninvasive biomarker comparatively high

Language: Английский

Citations

0
Non-Coding RNAs in Cancer: Mechanistic Insights and Therapeutic Implications DOI

Arjumand John,

Nuha Almulla,

Noureddine Elboughdiri

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 266, P. 155745 - 155745

Published: Nov. 29, 2024

Language: Английский

Citations

0
Lnc_CXADR/miR-421/ATG7 as promising biomarkers relevant to hepatocellular carcinoma DOI
Dalia Abdel-Wahab Mohamed, Raghda M. Ghorab,

Soha Abuelela

et al.

Gene Reports, Journal Year: 2024, Volume and Issue: 38, P. 102114 - 102114

Published: Dec. 10, 2024

Language: Английский

Citations

0
Construction of a ceRNA network to reveal a vascular invasion associated prognostic model in hepatocellular carcinoma DOI Creative Commons

Yun Liu,

Lu Yang,

Mengsi Yu

et al.

Open Medicine, Journal Year: 2023, Volume and Issue: 18(1)

Published: Jan. 1, 2023

Abstract The aim of this study is to explore the prognostic value vascular invasion (VI) in hepatocellular carcinoma (HCC) by searching for competing endogenous RNAs (ceRNA) network and constructing a new model HCC. differentially expressed genes (DEGs) between HCC normal tissues were identified from GEO TCGA. StarBase miRanda prediction tools applied construct circRNA-miRNA-mRNA network. DEGs with without VI also identified. Then, hub screened build risk score through method least absolute shrinkage selection operator. ability was assessed using Kaplan−Meier Cox regression analysis. In result, there 221 up-regulated 47 down-regulated circRNAs (DEcircRNAs) compared tissue. A circRNA-related ceRNA established, containing 11 DEcircRNAs, 12 DEmiRNAs, 161 DEmRNAs. Meanwhile, another DEG analysis revealed 625 123 VI, then protein–protein interaction (PPI) built based on 122 VI-related DEGs. From intersection within PPI networks, we obtained seven novel model. patients high-risk scores had shorter survival time presented more advanced T/N/M stages as well occurrence. conclusion VI-associated constructed study, great predict outcome patients.

Language: Английский

Citations

1
miR-550a-5p promotes the proliferation and migration of hepatocellular carcinoma by targeting GNE via the Wnt/β-catenin signaling pathway DOI Creative Commons
Chunming Li,

Wu Wu,

Zhu Zhu

et al.

Neoplasma, Journal Year: 2022, Volume and Issue: 69(06), P. 1359 - 1372

Published: Jan. 1, 2022

Liver cancer is one of the most common tumors with a high malignant degree in world. Its diagnosis and treatment are very difficult limited. More novel powerful DAT strategies urgently needed to break this situation. An increasing number studies have shown that microRNAs (miRNAs) could be used not only as biomarkers for prognosis hepatocellular carcinoma (HCC) but also important targets molecular targeted therapy. However, role miR-550a-5p HCC its specific mechanism remain unclear. Here we proposed verified hypothesis regulate progression was positively associated poor prognosis. We found decreased would inhibit proliferation migration cell lines (HCs) by performing relevant assays. Interestingly, knocking down GNE reverse above effect on HCs. Meanwhile, western blot results showed Wnt/β-catenin signaling pathway at least partly involved regulation miR-550a-5p. In addition, suppress growth vivo via xenograft tumor model assay. All all, draw conclusion miR-550a-5p/GNE axis functioned an promoting pathway.

Language: Английский

Citations

2