Exosomes derived from bone marrow mesenchymal stem cell preconditioned by low-intensity pulsed ultrasound stimulation promote bone–tendon interface fibrocartilage regeneration and ameliorate rotator cuff fatty infiltration DOI Creative Commons
Bing Wu, Tao Zhang, Huabin Chen

et al.

Journal of Orthopaedic Translation, Journal Year: 2024, Volume and Issue: 48, P. 89 - 106

Published: Aug. 2, 2024

Fibrovascular scar healing of bone-tendon interface (BTI) instead functional fibrocartilage regeneration is the main concern associated with unsatisfactory prognosis in rotator cuff repair. Mesenchymal stem cells (MSCs) exosomes have been reported to be a new promising cell-free approach for healing. Whereas, controversies abound whether native MSCs alone can effectively induce chondrogenesis. To explore effect derived from low-intensity pulsed ultrasound stimulation (LIPUS)-preconditioned bone marrow mesenchymal (LIPUS-BMSC-Exos) or un-preconditioned BMSCs (BMSC-Exos) on and underlying mechanism. C57BL/6 mice underwent unilateral supraspinatus tendon detachment repair were randomly assigned saline, BMSCs-Exos LIPUS-BMSC-Exos injection therapy. Histological, immunofluorescent biomechanical tests detected investigate BTI muscle fatty infiltration repaired cuff. In vitro, incubated BMSC-Exos then chondrogenic/adipogenic differentiation observed. Further, quantitative real-time polymerase chain reaction (qRT-PCR) was performed detect chondrogenesis/adipogenesis-related miRNA profiles BMSC-Exos. The potential key verified through function recover test its mimic inhibitor. results indicated that properties tendon-humeral junction significantly improved group than group. also exhibited higher histological score more newly regenerated at site postoperative 2 4 weeks less co-culture could promote chondrogenic inhibit adipogenic differentiation. Subsequently, qRT-PCR revealed enrichment miRNAs compared Moreover, we demonstrated this chondrogenesis-inducing primarily attributed miR-140, one most abundant LIPUS-BMSC-Exos. LIPUS-preconditioned ameliorate by positive regulation pro-chondrogenesis anti-adipogenesis, which delivering miR-140. These findings propose an innovative "LIPUS combined Exosomes strategy" combines both physiotherapeutic biotherapeutic advantages. This strategy possesses good translational as local LIPUS preconditioned BMSC-derived Exos during operation not only efficient promoting ameliorating infiltration, but time-saving, simple convenient patients.

Language: Английский

Revitalizing Ancient Mitochondria with Nano‐Strategies: Mitochondria‐Remedying Nanodrugs Concentrate on Disease Control DOI

Xingyu Long,

Min Liu,

Yayun Nan

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(18)

Published: Jan. 15, 2024

Abstract Mitochondria, widely known as the energy factories of eukaryotic cells, have a myriad vital functions across diverse cellular processes. Dysfunctions within mitochondria serve catalysts for various diseases, prompting widespread demise. Mounting research on remedying damaged indicates that constitute valuable target therapeutic intervention against diseases. But less clinical practice and lower recovery rate imply limitation traditional drugs, which need further breakthrough. Nanotechnology has approached favorable regiospecific biodistribution high efficacy by capitalizing excellent nanomaterials targeting drug delivery. Mitochondria‐remedying nanodrugs achieved ideal effects. This review elucidates significance in cells organs, while also compiling mortality data related Correspondingly, nanodrug‐mediate strategies applicable mitochondria‐remedying disease are detailed, with full understanding roles dysfunction advantages nanodrugs. In addition, future challenges directions discussed. conclusion, this provides comprehensive insights into design development nanodrugs, aiming to help scientists who desire extend their fields engage interdisciplinary subject.

Language: Английский

Citations

29
Mesenchymal stem cell-derived extracellular vesicles: a possible therapeutic strategy for orthopaedic diseases: a narrative review. DOI
Zhaolin Zeng, Hui Xie

PubMed, Journal Year: 2022, Volume and Issue: 3(3), P. 175 - 187

Published: Jan. 1, 2022

Accumulating evidence suggests that the therapeutic role of mesenchymal stem cells (MSCs) in bone diseases is closely related to paracrine-generated extracellular vesicles (EVs). MSC-derived EVs (MSC-EVs) carry proteins, nucleic acids, and lipids space affect microenvironment. They have similar biological functions MSCs, such as ability repair organ tissue damage. In addition, MSC-EVs also advantages long half-life, low immunogenicity, attractive stability, pass through blood-brain barrier, demonstrate excellent performance with potential practical applications diseases. this review, we summarise current mechanisms osteoporosis, osteoarthritis, tumours, osteonecrosis femoral head, fractures, well development combined materials science field orthopaedics. Additionally, explore critical challenges involved clinical application orthopaedic

Language: Английский

Citations

60
Mesenchymal Stem/Stromal Cells Derived from Human and Animal Perinatal Tissues—Origins, Characteristics, Signaling Pathways, and Clinical Trials DOI Creative Commons
Magdalena Kulus, Rafał Sibiak, Katarzyna Stefańska

et al.

Cells, Journal Year: 2021, Volume and Issue: 10(12), P. 3278 - 3278

Published: Nov. 23, 2021

Mesenchymal stem/stromal cells (MSCs) are currently one of the most extensively researched fields due to their promising opportunity for use in regenerative medicine. There many sources MSCs, which perinatal origin appear be an invaluable pool. Compared embryonic stem cells, they devoid ethical conflicts because derived from tissues surrounding fetus and can safely recovered medical waste after delivery. Additionally, MSCs exhibit better self-renewal differentiation properties than those adult tissues. It is important consider anatomy general description including isolation, differentiation, characterization different types both animals humans (placenta, umbilical cord, amniotic fluid). Ultimately, signaling pathways essential regarding clinical applications MSCs. these referring anatomical structure organs origin, when describing specific characteristics as well involved differentiation.

Language: Английский

Citations

59
Effect of polystyrene nanoplastics and their degraded forms on stem cell fate DOI
Gwang‐Bum Im,

Young Geon Kim,

In-Seong Jo

et al.

Journal of Hazardous Materials, Journal Year: 2022, Volume and Issue: 430, P. 128411 - 128411

Published: Feb. 2, 2022

Language: Английский

Citations

45
Understanding molecular characteristics of extracellular vesicles derived from different types of mesenchymal stem cells for therapeutic translation DOI Creative Commons
Zuo Ding, Zachary Greenberg,

Maria Fernanda Serafim

et al.

Extracellular Vesicle, Journal Year: 2024, Volume and Issue: 3, P. 100034 - 100034

Published: March 2, 2024

Mesenchymal stem cells (MSCs) have been studied for decades as candidates cellular therapy, and their secretome, including secreted extracellular vesicles (EVs), has identified to contribute significantly regenerative reparative functions. Emerging evidence suggested that MSC-EVs alone, could be used therapeutics emulate the biological function of MSCs. However, just with MSCs, shown vary in composition, depending on tissue source MSCs well protocols employed culturing obtaining EVs. Therefore, importance careful choice cell sources culture environments is receiving increasing attention. Many factors therapeutic potential MSC-EVs, tissue, isolation technique, conditions. This review illustrates molecular landscape EVs derived from different types MSC along strategies. A thorough analysis publicly available omic datasets was performed advance precision understanding unique source-dependent characteristics. The tissue-specific protein miRNA-driven Reactome ontology reveal distinct patterns top pathways across adipose, bone marrow, umbilical MSC-EVs. Moreover, a meta-analysis assisted by an AI technique analyze published literature, providing insights into translation based tissues.

Language: Английский

Citations

13
Iron-Mediated Regulation in Adipose Tissue: A Comprehensive Review of Metabolism and Physiological Effects DOI
Xinyu Yang, Xianghong Wang, Zhe Yang

et al.

Current Obesity Reports, Journal Year: 2025, Volume and Issue: 14(1)

Published: Jan. 3, 2025

Language: Английский

Citations

1
Crosstalk Between Antioxidants and Adipogenesis: Mechanistic Pathways and Their Roles in Metabolic Health DOI Creative Commons
M.W. Fu,

Kyung‐Sik Yoon,

Joohun Ha

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 203 - 203

Published: Feb. 10, 2025

The interplay between oxidative stress and adipogenesis is a critical factor in the development of obesity its associated metabolic disorders. Excessive reactive oxygen species (ROS) disrupt key transcription factors such as peroxisome proliferator-activated receptor gamma (PPARγ) CCAAT/enhancer-binding protein alpha (C/EBPα), impairing lipid metabolism, promoting adipocyte dysfunction, exacerbating inflammation insulin resistance. Antioxidants, classified endogenous (e.g., glutathione, superoxide dismutase, catalase) exogenous polyphenols, flavonoids, vitamins C E), are pivotal mitigating these effects by restoring redox balance preserving functionality. Endogenous antioxidants neutralize ROS safeguard cellular structures; however, under heightened stress, defenses often insufficient, necessitating dietary supplementation. Exogenous derived from plant-based sources, polyphenols vitamins, act through direct scavenging, upregulation antioxidant enzymes, modulation signaling pathways like nuclear kappa B (NF-κB) PPARγ, reducing peroxidation, inflammation, dysfunction. Furthermore, they influence epigenetic regulation transcriptional networks to restore differentiation limit accumulation. Antioxidant-rich diets, including Mediterranean diet, strongly with improved health, reduced rates, enhanced sensitivity. Advances personalized therapies, guided biomarkers supported novel delivery systems, present promising avenues for optimizing therapeutic interventions. This review, "Crosstalk Between Antioxidants Adipogenesis: Mechanistic Pathways Their Role Metabolic Health", highlights mechanistic which regulate enhance health.

Language: Английский

Citations

1
Print‐and‐Grow within a Novel Support Material for 3D Bioprinting and Post‐Printing Tissue Growth DOI Creative Commons
Majd Machour,

Noy Hen,

Idit Goldfracht

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 9(34)

Published: Oct. 19, 2022

Abstract 3D bioprinting holds great promise for tissue engineering, with extrusion in suspended hydrogels becoming the leading technique recent years. In this method, living cells are incorporated within bioinks, extruded layer by into a granular support material followed gelation of bioink through diverse cross‐linking mechanisms. This approach offers high fidelity and precise fabrication complex structures mimicking properties. However, transition cell mass mixed functional native‐like requires post‐printing cultivation vitro. An often‐overlooked drawback is nonuniform shrinkage deformation printed constructs during maturation period, to highly variable engineered unpredictable size shape. limitation poses challenge technology meet applicative requirements. A novel “print‐and‐grow,” involving subsequent κ ‐Carrageenan‐based microgels (CarGrow) days presented. CarGrow enhances long‐term structural stability objects providing mechanical support. Moreover, provides possibility live imaging monitor maturation. The “print‐and‐grow” method demonstrates accurate viability functionality while preserving construct's shape size.

Language: Английский

Citations

31
Fish scale-derived scaffolds with MSCs loading for photothermal therapy of bone defect DOI
Siyu Shen, Rui Liu, Chuanhui Song

et al.

Nano Research, Journal Year: 2023, Volume and Issue: 16(5), P. 7383 - 7392

Published: Feb. 22, 2023

Language: Английский

Citations

22
Assessing Tumorigenicity in Stem Cell-Derived Therapeutic Products: A Critical Step in Safeguarding Regenerative Medicine DOI Creative Commons
Zongjie Wang

Bioengineering, Journal Year: 2023, Volume and Issue: 10(7), P. 857 - 857

Published: July 19, 2023

Stem cells hold promise in regenerative medicine due to their ability proliferate and differentiate into various cell types. However, self-renewal multipotency also raise concerns about tumorigenicity during post-therapy. Indeed, multiple studies have reported the presence of stem cell-derived tumors animal models clinical administrations. Therefore, assessment is crucial evaluating safety therapeutic products. Ideally, needs be performed rapidly, sensitively, cost-effectively, scalable. This article reviews approaches for assessing tumorigenicity, including models, soft agar culture, PCR, flow cytometry, microfluidics. Each method has its advantages limitations. The selection assay depends on specific study stage development product. Combining assays may provide a more comprehensive evaluation tumorigenicity. Future developments should focus optimization standardization microfluidics-based methods, as well integration single platform efficient

Language: Английский

Citations

20