Stem Cell-Based Therapy and Cell-Free Therapy as an Alternative Approach for Cardiac Regeneration DOI Creative Commons
Iwona Deszcz

Stem Cells International, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 17

Published: Nov. 2, 2023

The World Health Organization reports that cardiovascular diseases (CVDs) represent 32% of all global deaths. ineffectiveness conventional therapies in CVDs encourages the development novel, minimally invasive therapeutic strategies for healing and regeneration damaged tissue. self-renewal capacity, multilineage differentiation, lack immunogenicity, immunosuppressive properties mesenchymal stem cells (MSCs) make them a promising option CVDs. However, growing evidence suggests myocardial occurs through paracrine factors extracellular vesicle (EV) secretion, rather than differentiation into cardiomyocytes. Research shows secrete or surface-shed their culture media various cytokines, chemokines, growth factors, anti-inflammatory EVs, which constitute an MSC-conditioned medium (MSC-CM) secretome. use MSC-CM enhances cardiac repair resident heart cell proliferation, scar mass reduction, decrease infarct wall thickness, function improvement comparable to MSCs without side effects. This review highlights limitations benefits based on secretome as innovative treatment

Language: Английский

Young Exosome Bio‐Nanoparticles Restore Aging‐Impaired Tendon Stem/Progenitor Cell Function and Reparative Capacity DOI
Shanshan Jin, Yu Wang,

Xiaolan Wu

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(18)

Published: Feb. 13, 2023

Aging impairs tendon stem/progenitor cell function and homeostasis, however, effective treatments for aging-induced diseases are lacking. Exosomes naturally derived nanoparticles that contain bioactive molecules, therefore, have attracted great interest in tissue engineering regenerative medicine. In this study, it is shown young exosomes secreted by stem cells from human exfoliated deciduous teeth (SHED-Exos) possess abundant anti-aging signals. These bio-nanoparticles can alleviate the aging phenotypes of aged (AT-SCs) maintain their tenogenic capacity. Mechanistically, SHED-Exos modulate histone methylation inhibit nuclear factor-κB to reverse AT-SC aging. a mouse model, systemic administration SHED-Exo retards degeneration. Interestingly, local delivery SHED-Exos-loaded microspheres confers phenotypes, including reduced senescent decreased ectopic bone formation, thereby functionally structurally rescuing endogenous regeneration repair capacity rats. Overall, SHED-Exos, as natural nanoparticles, promising translational therapeutic potential aging-related diseases.

Language: Английский

Citations

75
Mesenchymal stem cell secretome for regenerative medicine: Where do we stand? DOI Creative Commons
C. Trigo, Joana S. Rodrigues, S.P. Camões

et al.

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: 70, P. 103 - 124

Published: May 9, 2024

Mesenchymal stem cell (MSC)-based therapies have yielded beneficial effects in a broad range of preclinical models and clinical trials for human diseases. In the context MSC transplantation, it is widely recognized that main mechanism regenerative potential MSCs not their differentiation, with vivo data revealing transient low engraftment rates. Instead, therapeutic are mainly attributed to its secretome, i.e., paracrine factors secreted by these cells, further offering more attractive innovative approach due effectiveness safety cell-free product. this review, we will discuss benefits MSC-derived secretome medicine particular focus on respiratory, hepatic, neurological Both free vesicular be detailed. We also address novel strategies capable improving healing potential, namely delivering important molecules according specific diseases tissue needs, as well non-clinical studies allow us dissect mechanisms action. includes both soluble non-soluble factors, organized extracellular vesicles (EVs). Importantly, besides depending origin, characteristics deeply influenced external stimuli, highlighting possibility optimizing through preconditioning approaches. Nevertheless, clarity around action remains ambiguous, whereas need standardized procedures successful translation those products clinics urges.

Language: Английский

Citations

24
Application of dental pulp stem cells for bone regeneration DOI Creative Commons
Ye Liu, Wei Xiong, Junyi Li

et al.

Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Feb. 29, 2024

Bone defects resulting from severe trauma, tumors, inflammation, and other factors are increasingly prevalent. Stem cell-based therapies have emerged as a promising alternative. Dental pulp stem cells (DPSCs), sourced dental pulp, garnered significant attention owing to their ready accessibility minimal collection-associated risks. Ongoing investigations into DPSCs revealed potential undergo osteogenic differentiation capacity secrete diverse array of ontogenetic components, such extracellular vesicles cell lysates. This comprehensive review article aims provide an in-depth analysis secretory emphasizing extraction techniques utilization while elucidating the intricate mechanisms governing bone regeneration. Furthermore, we explore merits demerits cell-free therapeutic modalities, well discuss prospects, opportunities, inherent challenges associated with DPSC therapy in context

Language: Английский

Citations

9
Regenerative potential of concentrated growth factor compared to platelet-rich fibrin in treatment of necrotic mature teeth: a randomized clinical trial DOI Creative Commons
Taghreed Salah,

Wael Hussein,

Heba Abdelkafy

et al.

BDJ Open, Journal Year: 2025, Volume and Issue: 11(1)

Published: Feb. 3, 2025

Language: Английский

Citations

1
Potential of Oral Cavity Stem Cells for Bone Regeneration: A Scoping Review DOI Creative Commons

Josefa Alarcón-Apablaza,

Ruth Prieto, M. F. Rojas

et al.

Cells, Journal Year: 2023, Volume and Issue: 12(10), P. 1392 - 1392

Published: May 15, 2023

Bone loss is a common problem that ranges from small defects to large after trauma, surgery, or congenital malformations. The oral cavity rich source of mesenchymal stromal cells (MSCs). Researchers have documented their isolation and studied osteogenic potential. Therefore, the objective this review was analyze compare potential MSCs for use in bone regeneration.A scoping carried out following Preferred Reporting Items Systematic Reviews Meta-Analyses extension Scoping (PRISMA-ScR) guidelines. databases reviewed were PubMed, SCOPUS, Scientific Electronic Library Online (SciELO), Web Science. Studies using stem promote regeneration included.A total 726 studies found, which 27 selected. used repair (I) dental pulp permanent teeth, (II) derived inflamed pulp, (III) exfoliated deciduous (IV) periodontal ligament cells, (V) cultured autogenous periosteal (VI) buccal fat pad-derived (VII) autologous bone-derived cells. Stem associate with scaffolds facilitate insertion into defect enhance regeneration. biological risk morbidity MSC-grafted site minimal. Successful formation MSC grafting has been shown as well larger periosteum, bone, pad.Stem maxillofacial origin are promising alternative treat craniofacial defects; however, an additional scaffold complement required cell delivery.

Language: Английский

Citations

17
Hypoxia Induces DPSC Differentiation versus a Neurogenic Phenotype by the Paracrine Mechanism DOI Creative Commons
Simona Delle Monache, Fanny Pulcini, Francesca Santilli

et al.

Biomedicines, Journal Year: 2022, Volume and Issue: 10(5), P. 1056 - 1056

Published: May 3, 2022

As previously described by several authors, dental pulp stem cells (DPSCs), when adequately stimulated, may acquire a neuronal-like phenotype acting as favorable source of in the generation nerves. Besides, it is known that hypoxia conditioning capable stimulating cell differentiation well survival and self-renewal, multiple growth factors, including Epidermal Growth factor (EGF) basic fibroblast (bFGF), are often involved induction neuronal progenitor cells. In this work, we investigated role commitment DPSCs into phenotype. These were conditioned with (O2 1%) for 5 16 days; subsequently, analyzed proliferation rate morphology, tested neural markers. Moreover, verified possible autocrine/paracrine comparing secretome profile hypoxic normoxic media (CM). Our results showed hypoxia-mediated DPSC was time dependent. (CM derived from stimulated able, turn, to induce SH-SY5Y neuroblastoma undifferentiated DPSCs. conclusion, under herein-mentioned conditions, seems favor neuron-like way, confirm potential clinical utility differentiated DPSCs, also suggest even greater CM-derived-hypoxic could more readily be used regenerative therapies.

Language: Английский

Citations

25
Advances in oral mesenchymal stem cell-derived extracellular vesicles in health and disease DOI Creative Commons

Huanyu Luo,

Anahid A. Birjandi,

Feilong Ren

et al.

Genes & Diseases, Journal Year: 2023, Volume and Issue: 11(1), P. 346 - 357

Published: April 12, 2023

Extracellular vesicles (EVs) are nano-size secreted naturally by all cells into the extracellular space and have been recognized as important cell-cell mediators in multicellular organisms. EVs contain nucleic acids, proteins, lipids, other cellular components, regulating many basic biological processes playing an role regenerative medicine diseases. can be traced to their of origin exhibit a similar function. Moreover, demonstrate low immunogenicity, good biocompatibility, fewer side effects, compared parent cells. Mesenchymal stem (MSCs) one most resource for EVs, with great capacity self-renewal multipotent differentiation, play essential cell therapy. The mechanism MSC therapy was thought attributed differentiation MSCs after targeted migration, previously noted. However, emerging evidence shows unknown MSC-derived paracrine factors Especially derived from oral tissue (OMSC-EVs), show more advantages than those repair regeneration, due lower invasiveness easier accessibility sample collection. Here, we systematically review biogenesis characteristics OMSC-EVs, well OMSC-EVs intercellular communication. Furthermore, discuss potential therapeutic roles systemic We highlight current challenges future directions focus attention on clinical translation. aim provide valuable insights explorative application OMSC-EVs.

Language: Английский

Citations

14
Platelet-rich plasma-derived exosomes enhance mesenchymal stem cell paracrine function and nerve regeneration potential DOI
Yongyi Zhang, Dan Yi, Quan Hong

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 699, P. 149496 - 149496

Published: Jan. 8, 2024

Language: Английский

Citations

5
Engineered exosome therapeutics for neurodegenerative diseases DOI Creative Commons

Biplob Saikia,

Anandh Dhanushkodi

Life Sciences, Journal Year: 2024, Volume and Issue: 356, P. 123019 - 123019

Published: Aug. 28, 2024

An increase in life expectancy comes with a higher risk for age-related neurological and cognitive dysfunctions. Given the psycho-socioeconomic burden due to unhealthy aging coming decades, United Nations has declared 2021-2030 as decade of healthy aging. In this line, multipotent mesenchymal stromal cell-based therapeutics received special interest from research community. Based on decades cell therapy, consensus emerged that therapeutic effects therapy are paracrine mechanisms rather than replacement. Exosomes, constituent secretome, nano-sized vesicles have been focus intense recent years possible agent or cargo deliver drugs into central nervous system induce neurogenesis, reduce neuroinflammation, confer neuroregeneration/neuroprotection, improve motor functions. review, we discussed neuroprotective properties exosomes derived adult stem cells, role exosomal miRNAs. We also reviewed various strategies exosome production their content better effects. Further, utilization ectomesenchymal cells like dental pulp treating neurodegenerative diseases.

Language: Английский

Citations

5
Immortalization of Mesenchymal Stem Cells for Application in Regenerative Medicine and Their Potential Risks of Tumorigenesis DOI Open Access

Natsuki Yamaguchi,

Eri Horio,

Jukito Sonoda

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13562 - 13562

Published: Dec. 18, 2024

Regenerative medicine utilizes stem cells to repair damaged tissues by replacing them with their differentiated and activating the body’s inherent regenerative abilities. Mesenchymal (MSCs) are adult that possess tissue capabilities immunomodulatory properties a much lower risk of tumorigenicity, making focus numerous clinical trials worldwide. MSCs primarily exert therapeutic effects through paracrine via secreted factors, such as cytokines exosomes. This has led increasing interest in cell-free therapy, where only conditioned medium (also called secretome) from MSC cultures is used for applications. However, face certain limitations, including cellular senescence, scarcity, donor heterogeneity, complexity, short survival post-implantation, regulatory ethics hurdles. To address these challenges, various types immortalized (ImMSCs) capable indefinite expansion have been developed. These offer significant promise essential tools reliable source both cell-based therapies aim translating into practical medicine. process immortalization, often involving transduction immortalizing genes, poses potential risks genetic instability resultant malignant transformation. Cell-free therapy particularly attractive, it circumvents tumorigenicity ethical concerns associated live cell therapies. Rigorous safety tests, monitoring chromosomal abnormalities, critical ensure safety. Technologies like inducible or suicide genes may allow controlled proliferation induce apoptosis after task completed. review highlights recent advancements immortalization tumorigenesis.

Language: Английский

Citations

5