CEP76 is a critical regulator of male germ cell transition zone function and tail composition DOI Creative Commons
Brendan J. Houston, D. Jo Merriner, G. Gemma Stathatos

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: March 9, 2023

Abstract The transition zone is a specialised gate at the base of cilia/flagella, which segregates ciliary compartment from cytoplasm and strictly regulates protein entry. In this study, we have identified CEP76 as an essential germ cell protein, involved in selective entry incorporation key proteins required for sperm function fertility into ultimately tail. its absence tails are shorter immotile consequence deficits motility including DNAH2 AKAP4, accumulate neck mutant. We demonstrate tail fibrous sheath formation, outer dense fibre loading axoneme stability principal piece motility. Finally, identify that dictates annulus positioning composition, adding further evidence spermatid part same functional structure.

Language: Английский

The Cby3/ciBAR1 complex positions the annulus along the sperm flagellum during spermiogenesis DOI Creative Commons
Mohammed Hoque, Feng‐Qian Li, William Weber

et al.

The Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 223(3)

Published: Jan. 10, 2024

Proper compartmentalization of the sperm flagellum is essential for fertility. The annulus a septin-based ring that demarcates midpiece (MP) and principal piece (PP). It assembled at flagellar base, migrates caudally, halts upon arriving PP. However, mechanisms governing positioning remain unknown. We report Chibby3 (Cby3)/Cby1-interacting BAR domain-containing 1 (ciBAR1) complex required this process. Ablation either gene in mice results male fertility defects, caused by kinked flagella with mispositioned Cby3 ciBAR1 interact colocalize to near curved membrane invagination pocket. In absence Cby3, periannular membranes appear be deformed, allowing migrate over fibrous sheath into Collectively, our suggest Cby3/ciBAR1 regulates local properties position MP/PP junction.

Language: Английский

Citations

4
Impact of Air Pollution and Heavy Metal Exposure on Sperm Quality: A Clinical Prospective Research Study DOI Creative Commons

D Abilash,

T.B. Sridharan

Toxicology Reports, Journal Year: 2024, Volume and Issue: 13, P. 101708 - 101708

Published: Aug. 8, 2024

Exposure to air pollution poses significant risks human health, including detrimental effects on the reproductive system, affecting both men and women. Our prospective clinical study aimed assess impact of prolonged exposure sperm quality in male patients attending a fertility clinic. The current was conducted at Sri Narayani Hospital Research Centre Vellore, Tamil Nadu, India, examined samples obtained from individuals with extended pollution. Microscopic analysis, scanning electron microscopy (SEM), evaluate morphology. At same time, atomic absorption spectroscopy (AAS) determined presence heavy metals, Zinc (Zn), Magnesium (Mg), Lead (Pb) Cadmium (Cd), known affect production. findings revealed that long-term adversely affects quality, manifesting alterations during spermatogenesis cycle, morphological abnormalities observed through SEM, impaired motility. Additionally, epidemiological evidence suggests elevated levels cadmium lead environment induce oxidative stress, leading DNA damage reduced concentrations. These results underscore urgent need for environmental interventions mitigate protect health.

Language: Английский

Citations

4
State-of-the-art and future perspectives in infertility diagnosis: Conventional versus nanotechnology-based assays DOI

Bianca-Astrid Andone,

Iuliana M. Handrea-Dragan, Ioan Botiz

et al.

Nanomedicine Nanotechnology Biology and Medicine, Journal Year: 2023, Volume and Issue: 54, P. 102709 - 102709

Published: Sept. 16, 2023

Language: Английский

Citations

8
Genetic mutation ofCep76results in male infertility due to abnormal sperm tail composition DOI Creative Commons
Brendan J. Houston, D. Jo Merriner, G. Gemma Stathatos

et al.

Life Science Alliance, Journal Year: 2024, Volume and Issue: 7(6), P. e202302452 - e202302452

Published: April 3, 2024

The transition zone is a specialised gate at the base of cilia/flagella, which separates ciliary compartment from cytoplasm and strictly regulates protein entry. We identified potential new regulator male germ cell zone, CEP76. demonstrated that CEP76 was involved in selective entry incorporation key proteins required for sperm function fertility into ultimately tail. In mutant, tails were shorter immotile as consequence deficits essential motility including DNAH2 AKAP4, accumulated neck mutant. Severe annulus, fibrous sheath, outer dense fibre abnormalities also detected lacking Finally, we dictates annulus positioning structure. This study suggests adds further evidence to hypothesis spermatid are part same functional

Language: Английский

Citations

2
Sperm functionality is differentially regulated by porcine oviductal extracellular vesicles from the distinct phases of the estrous cycle DOI
S. M. Toledo-Guardiola, Pablo Martínez‐Díaz, R Martinez-Nunez

et al.

Reproduction Fertility and Development, Journal Year: 2024, Volume and Issue: 36(8)

Published: May 7, 2024

Context Extracellular vesicles (EVs) derived from the oviductal fluid (oEVs) play a critical role in various reproductive processes, including sperm capacitation, fertilisation, and early embryo development. Aims To characterise porcine oEVs (poEVs) different stages of estrous cycle (late follicular, LF; luteal, EL; mid ML; late LL) investigate their impact on functionality. Methods poEVs were isolated, characterised, labelled to assess binding boar spermatozoa. The effects motility, viability, acrosomal status, protein kinase A phosphorylation (pPKAs), tyrosine (Tyr-P), vitro fertility analysed. Key results observed as round or cup-shaped membrane-surrounded vesicles. Statistical analysis showed that did not significantly differ size, quantity, concentration among phases cycle. However, LF demonstrated higher affinity for sperm. Treatment with EL, ML, LL resulted decrease progressive motility total motility. Moreover, pPKA levels reduced presence LF, ML poEVs, while Tyr-P between groups. also penetration rate number spermatozoa per penetrated oocyte (P < 0.05). Conclusions modulatory functionality by interacting spermatozoa, affecting participating sperm–oocyte interaction. Implications differential suggest potential use additives IVF systems regulate

Language: Английский

Citations

2
AXDND1 is required to balance spermatogonial commitment and for sperm tail formation in mice and humans DOI Creative Commons
Brendan J. Houston, Joseph Nguyen, D. Jo Merriner

et al.

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(7)

Published: July 12, 2024

Abstract Dynein complexes are large, multi-unit assemblies involved in many biological processes via their critical roles protein transport and axoneme motility. Using next-generation sequencing of infertile men presenting with low or no sperm ejaculates, we identified damaging variants the dynein-related gene AXDND1 . We thus hypothesised that is a regulator male fertility. To test this hypothesis, produced knockout mouse model. Axdnd1 −/− males were sterile at all ages but presented an evolving testis phenotype wherein they could undergo one round histologically replete spermatogenesis followed by rapid depletion seminiferous epithelium. Marker experiments role for maintaining balance between differentiation-committed self-renewing spermatogonial populations, resulting disproportionate production differentiating cells absence increased during initial spermatogenic waves. Moreover, long-term maintenance was compromised, ultimately leading to catastrophic germ cell loss, destruction blood–testis barrier integrity immune infiltration. In addition, first wave immotile due abnormal structure, including presence ectopic vesicles abnormalities outer dense fibres microtubule doublet structures. Sperm output additionally compromised severe spermiation defect individualisation. Collectively these data identify as atypical dynein complex-related protein/vesicle relevance function tail formation mice humans. This study underscores importance studying consequences loss-of-function on both establishment

Language: Английский

Citations

2
The seminal plasma proteins Peptidyl arginine deaminase 2, rRNA adenine N (6)-methyltransferase and KIAA0825 are linked to better motility post thaw in stallions DOI
Gemma Gaitskell‐Phillips, Francisco E. Martín‐Cano, José M. Ortiz-Rodríguez

et al.

Theriogenology, Journal Year: 2021, Volume and Issue: 177, P. 94 - 102

Published: Oct. 15, 2021

Language: Английский

Citations

11
Critical role of Spatio-Temporally Regulated Maternal RNAs in Zebrafish Embryogenesis DOI Creative Commons
Gopal Kushawah,

Danielson Baia Amaral,

Huzaifa Hassan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Summary The maternal-to-zygotic transition shifts regulatory control from maternal to zygotic messenger RNAs (mRNA) through mRNA degradation. While temporal aspects of decay are known, spatial mechanisms remain underexplored. Using CRISPR-Cas9 and CRISPR-Cas13d systems, we functionally dissected the contribution versus fractions overcame challenges studying embryonic lethal genes. We identified differentially distributed mRNAs in specific cells evidenced critical role five mRNAs, cth1, arl4d, abi1b, foxa lhx1a, embryogenesis. Further, focused on uncharacterized cth1 gene, revealing its essential gametogenesis Cth1 acts as a spatio-temporal RNA factor regulating stability accumulation targets manner 3’UTR recognition during early development. Furthermore, drives localization. Our findings provide new insights into highlight dual CRISPR-Cas strategies Highlights Differentially marginal have Cas13d complements Cas9 limitation study functions is for required regulation. dynamics.

Language: Английский

Citations

1
AXDND1 is required to balance spermatogonial commitment and for sperm tail formation in mice and humans DOI Creative Commons
Brendan J. Houston, Joseph Nguyen, D. Jo Merriner

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 4, 2023

Abstract Dynein complexes are large, multi-unit assemblies involved in many biological processes including male fertility via their critical roles protein transport and axoneme motility. Previously we identified a pathogenic variant the dynein gene AXDND1 an infertile man. Subsequently additional four potentially compound heterozygous variants of unknown significance two men. We thus tested role mammalian by generating knockout mouse model. Axdnd1 -/- males were sterile at all ages but could undergo one round histologically complete spermatogenesis. Subsequently, progressive imbalance spermatogonial commitment to spermatogenesis over self-renewal occurred, ultimately leading catastrophic germ cell loss, loss blood-testis barrier patency immune infiltration. Sperm produced during first wave immotile due abnormal structure, presence ectopic vesicles abnormalities outer dense fibres microtubule doublet structures. output was additionally compromised severe spermiation defect sperm individualisation. Collectively, our data highlight essential as regulator spermiogenesis where it is for tail development, release

Language: Английский

Citations

2
The Effect of Cryopreservation on the Sperm Ultrastructure of Mus Musculus Albinus Strain DDY: Comparison of Nakagata vs Modified vs Kitazato Cryoprotectants DOI Open Access

Manggiasih Dwiayu Larasati,

Silvia Werdhy Lestari, Mulyoto Pangestu

et al.

Pharmacognosy Journal, Journal Year: 2024, Volume and Issue: 16(3), P. 563 - 569

Published: July 1, 2024

Introduction: Sperm morphology analysis is very necessary to understand male fertility and the etiology of infertility. Currently, scanning electron microscopy (SEM) has been widely used determine surface topology. In this study, we will compare effects spermatozoa cryopreservation using three different types cryoprotectants, namely Nakagata, modification Kitazato. The cryoprotectant compositions are Nakagata (raffinose skim milk), modified (glycerol raffinose) Kitazato trehalose). Methods: SEM was carried out on 8 sperm samples before after freeze-thaw process. Results: results obtain showed that able protect better than Analysis revealed damage plasma membrane, acrosome loss mitochondria in all treatment groups compared fresh sperm. obvious signs post-thaw such as missing membranes, showing damaged acrosomes middle structural disorganization. Conclusion: caused ultrastructural mice due freezing thawing. These details provide valuable data for further research minimize by Apart from that, examination TEM recommended a more comprehensive picture.

Language: Английский

Citations

0