Regeneration of Lumbriculus variegatus requires post‐amputation production of reactive oxygen species

Development Growth & Differentiation, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Abstract Animals vary in their ability to replace body parts lost injury, a phenomenon known as restorative regeneration. Uncovering conserved signaling steps required for regeneration may aid regenerative medicine. Reactive oxygen species (ROS) are necessary proper across wide range of taxa, but it is unknown whether ROS essential annelid As annelids widely used and excellent model regeneration, we sought determine play role the highly annelid, Lumbriculus variegatus . Using ROS‐sensitive fluorescent probe observed accumulation at wound site within 15 min after amputation; this burst lessened by 6 h post‐amputation. Chemical inhibition delayed an impairment that was partially rescued with exogenous ROS. Our results suggest similar other animals, depends upon signaling, implying phylogenetically ancient requirement

Language: Английский

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Impacts of severity and region of gill tissue resection on regeneration in Atlantic salmon (Salmo salar)

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 111815 - 111815

Published: Jan. 1, 2025

Language: Английский

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Characterization of regeneration initiating cells during Xenopus laevis tail regeneration

Genome biology, Journal Year: 2024, Volume and Issue: 25(1)

Published: Sept. 30, 2024

Language: Английский

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Wide-scale identification of novel/eliminated genes responsible for evolutionary transformations

Biology Direct, Journal Year: 2023, Volume and Issue: 18(1)

Published: Aug. 11, 2023

It is generally accepted that most evolutionary transformations at the phenotype level are associated either with rearrangements of genomic regulatory elements, which control activity gene networks, or changes in amino acid contents proteins. Recently, evidence has accumulated significant could also be loss/emergence whole genes. The targeted identification such genes a challenging problem for both bioinformatics and evo-devo research.To solve this we propose WINEGRET method, named after first letters title. Its main idea to search satisfy two requirements: first, desired were lost/emerged same stage phenotypic trait interest was lost/emerged, second, expression these significantly during development model organism. To verify requirement, do not use existing databases orthologs, but rely purely on homology local synteny by using some novel quickly computable conditions. Genes satisfying second requirement found deep RNA sequencing. As proof principle, used our method find absent extant amniotes (reptiles, birds, mammals) present anamniotes (fish amphibians), involved regeneration large body appendages. result, 57 identified. For three them, c-c motif chemokine 4, eotaxin-like, previously unknown called here sod4, essential roles tail demonstrated. Noteworthy, established latter belongs family Cu/Zn-superoxide dismutases lost amniotes, SOD4.We whose evolution interest. In proof-of-principle study, identified participate appendage anamniotes. Our provides wide range opportunities studying relationship between traits specific evolution.

Language: Английский

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Characteristics of a gill resection – Regeneration model in freshwater laboratory-reared Atlantic salmon (Salmo salar)

Aquaculture, Journal Year: 2023, Volume and Issue: 579, P. 740210 - 740210

Published: Oct. 12, 2023

Language: Английский

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Conservatism and Variability of the Antioxidant Defense System in the Retinal Pigment Epithelium of Vertebrates

Journal of Evolutionary Biochemistry and Physiology, Journal Year: 2023, Volume and Issue: 59(3), P. 655 - 675

Published: May 1, 2023

Language: Английский

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Antibacterial Activity of AXOTL-13, a Novel Peptide Identified from the Transcriptome of the Salamander Ambystoma mexicanum

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(11), P. 1445 - 1445

Published: Nov. 12, 2024

: Antimicrobial peptides are essential molecules in the innate immunity of various organisms and possess a broad spectrum antimicrobial, antitumor, immunomodulatory activities. Due to their multifunctionality, they seen as an alternative for controlling bacterial infections. Although conventional antibiotics have improved health worldwide, indiscriminate use has led emergence resistant microorganisms. To discover new with antimicrobial activity that could overcome limitations traditional antibiotics, this study aimed identify

Language: Английский

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Cellular and Molecular Triggers of Retinal Regeneration in Amphibians

Life, Journal Year: 2023, Volume and Issue: 13(10), P. 1981 - 1981

Published: Sept. 28, 2023

Understanding the mechanisms triggering initiation of retinal regeneration in amphibians may advance quest for prevention and treatment options degenerating human retina diseases. Natural requires two cell sources, namely pigment epithelium (RPE) ciliary marginal zone. The disruption RPE interaction with photoreceptors through surgery or injury triggers local systemic responses protection. In mammals, disease-induced damage to results shutdown function, cellular oxidative stress, pronounced immune response, death degeneration. contrast pathology regenerative have taxon-specific features ensuring efficient regeneration. These include rapid hemostasis, recruitment cells factors endogenous defense systems, activities immature system, high viability, efficiency extracellular matrix, cytoskeleton, surface remodeling. reactions are controlled by specific signaling pathways, transcription factors, epigenome, which insufficiently studied. This review provides a summary initiating reveals its collectively directed at recruiting universal trauma activate sources study integrated molecular network these processes is prospect future research demand biomedicine.

Language: Английский

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Molecular Signatures Integral to Natural Reprogramming in the Pigment Epithelium Cells after Retinal Detachment in Pleurodeles waltl

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(23), P. 16940 - 16940

Published: Nov. 29, 2023

The reprogramming of retinal pigment epithelium (RPE) cells into (transdifferentiation) lies in the bases regeneration several Urodela. identification key genes involved this process helps with looking for approaches to prevention and treatment RPE-related degenerative diseases human retina. purpose our study was examine transcriptome changes at initial stages RPE cell adult newt Pleurodeles waltl. isolated from eye samples day 0, 4, 7 after experimental surgical detachment neural retina used a de novo assembly through RNA-Seq method. A total 1019 transcripts corresponding differently expressed have been revealed silico: 83 increased expression an early stage, 168 late stage reprogramming. We identified up-regulation classical response genes, chaperones co-chaperones, regulation protein biosynthesis, suppressors oncogenes, EMT-related genes. growth proportion down-regulated ribosomal translation-associated Our findings contribute revealing molecular mechanism

Language: Английский

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Protein tyrosine phosphatases in zebrafish development and regeneration

Published: Dec. 4, 2023

Protein tyrosine phosphatases (PTPs) have a central role in cell signaling, by dephosphorylating other proteins. PTPs are highly susceptible to oxidation, due the low pKa of their active sites. Their reactive cysteine gets oxidized, which leads temporary inactivation PTP. It has been shown many different species that burst Reactive Oxygen Species (ROS) is produced upon injury. This ROS plays an important wound healing and regeneration. We hypothesized could start signaling cascade oxidizing PTPs. Previously, eight were identified specifically oxidized response amputation tail fin adult zebrafish. One these PTPs, Shp2a, was be essential for In chapter 2 we describe how generated zebrafish knockouts 6 previously caudal paralogs. show not addition, did recapitulate all phenotypes expected. hypothesize this genetic compensation. 3 our investigation with knockout ptpn4a. majority mutant died at juvenile stage suffered from epileptic seizures. corresponds human patients who suffer intellectual disability seizures nonsense mutations PTPN4. minority compensated loss Ptpn4a survived, but also separate line lost its compensation ability over time showed enhanced lethality stage. 4 ptpn6. displayed fully penetrant mortality larval lethal hyperinflammation. phenotype motheaten mice lacking SHP1 suffering neutrophilic dermatoses emphysema. Shp1 led reduced number emerging HSPCs, increased HSPC proliferation, macrophages decreased neutrophils. affected behavior neutrophils directional migration wounding. 5 investigated oxidation Shp2a during regeneration, creating fusion protein consisting linked Catalase. Due close vicinity, Catalase protected oxidation. inhibiting inhibited indicates transient step results research puts end apparent paradox catalytic activity regeneration proceed. reduction negative feedback loop, resulting once again.

Language: Английский

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