Phage therapy minimally affects the water microbiota in an Atlantic salmon (Salmo salar) rearing system while still preventing infection DOI Creative Commons
Alexander W. Fiedler, Madeleine S. Gundersen,

Toan P. Vo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 4, 2023

Abstract Excessive usage of antibiotics threatens the bacterial diversity in microbiota animals. An alternative to that has been suggested not disturb is (bacterio)phage therapy. In this study, we challenged germ-free and microbially colonized yolk sac fry Atlantic salmon with Flavobacterium columnare observed mere presence a protected fish against lethal infection. We then investigated effect phage-or oxytetracycline treatment on survival rearing water community characteristics using 16S rRNA gene amplicon sequencing. Phage led an increased F. columnare- reduced relative amounts pathogen microbiota. absence , phage did affect composition or α-diversity phage’s host, induced minor changes composition, without affecting α-diversity. Surprisingly, had no observable reduce abundance water. conclusion, showed prevents mortality while negatively microbiota, thus suggesting may be suitable antibiotics. also demonstrated protective fry.

Language: Английский

Community assembly of gut microbiomes in yolk sac fry of Atlantic salmon: host genetics, environmental microbiomes, and ecological processes DOI Creative Commons

Amalie Johanne Horn Mathisen,

Sol Gómez de la Torre Canny, Madeleine S. Gundersen

et al.

FEMS Microbiology Ecology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

In this study, we investigated the influence of host genetics and environmental microbiomes on early gut microbiome Atlantic salmon. We aimed at rearing fish in either r- or K-selected environments, where r-selected environment would be expected to dominated by fast-growing opportunistic bacteria thus represent more detrimental microbial than water. Eggs from both wild aquaculture strains salmon were hatched under germ-free conditions. One week after hatching (wph), flasks inoculated with water communities. Three wph, no effect strain observed. r-selection was found take place all flasks, including added Still, differed significantly between that had been (Add-r Add-K respectively). Lower alpha diversity higher abundances Pseudomonas observed for indicating an unfavorable environment. Selection structured microbiomes, but extensive intraindividual variation explained stochastic processes community assembly. The also Add-r flasks. they similarities assembly communities less influenced processes. lower growth rates probably a result negative host-microbe interactions. These findings highlight importance of, challenges related to, managing when cultivating fish.

Language: Английский

Citations

0
Fish as biological models: Implications for gut microbiota research in biomedical and aquaculture sciences DOI
Diana Medina‐Félix, Estefanía Garibay‐Valdez, Francisco Vargas‐Albores

et al.

Aquaculture International, Journal Year: 2025, Volume and Issue: 33(4)

Published: May 2, 2025

Language: Английский

Citations

0
Interwoven processes in fish development: microbial community succession and immune maturation DOI Creative Commons

Lisa Zoé Auclert,

Mousumi Sarker Chhanda, Nicolas Derôme

et al.

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e17051 - e17051

Published: March 27, 2024

Fishes are hosts for many microorganisms that provide them with beneficial effects on growth, immune system development, nutrition and protection against pathogens. In order to avoid spreading of infectious diseases in aquaculture, prevention includes vaccinations routine disinfection eggs equipment, while curative treatments consist the administration antibiotics. Vaccination processes can stress fish require substantial farmer’s investment. Additionally, antibiotics not specific, they may be effective short term, have major drawbacks long term. Indeed, eliminate bacteria which useful host promote raising antibiotic resistance beneficial, commensal but also pathogenic bacterial strains. Numerous publications highlight importance plays diversified microbial community colonizing (i.e., microbiota) health ultimately survival their host. This review targets current knowledge bidirectional communication between microbiota during development. It explores extent this mutualistic relationship: one hand, effect microbes exert ontogeny fishes, other impact critical steps development recruitment succession throughout life. We will first describe its gene expression fishes. Secondly, plurality microbiotas (depending organism, organ, stage) reviewed. Then, a description constant interactions fish’s life stages discussed. Healthy allow maturation modulation inflammation, both contribute homeostasis. Thus, equilibrium is closely linked stability examples from several species more extensively mechanisms occurring zebrafish model because much finely described species, thanks existing mutants precise investigations. conclude how conceptual framework associated research benefit considering relations maturation. More precisely, active tolerance earliest enables sustainable establishment complex healthy adult Establishing balanced host-microbiota interaction avoids triggering deleterious maintains immunological microbiological

Language: Английский

Citations

2
A brain microbiome in salmonids at homeostasis DOI Creative Commons
Amir Mani, C. Henn, Claire E. Couch

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(38)

Published: Sept. 18, 2024

Ectotherms have peculiar relationships with microorganisms. For instance, bacteria are recovered from the blood and internal organs of healthy teleosts. However, presence microbial communities in teleost brain has not been proposed. Here, we report a living bacterial community salmonids loads comparable to those spleen 1000-fold lower than gut. Brain share >50% their diversity gut communities. Using culturomics, obtained 54 isolates brains trout. Comparative genomics suggests that may adaptations for niche colonization polyamine biosynthesis. In natural system, Chinook salmon microbiomes shift juveniles reproductively mature adults. Our study redefines physiological between This symbiosis endow direct mechanism sense respond environmental microbes.

Language: Английский

Citations

2
Phage therapy minimally affects the water microbiota in an Atlantic salmon (Salmo salar) rearing system while still preventing infection DOI Creative Commons
Alexander W. Fiedler, Madeleine S. Gundersen,

Toan P. Vo

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Nov. 6, 2023

Excessive usage of antibiotics threatens the bacterial diversity in microbiota animals. An alternative to that has been suggested not disturb is (bacterio)phage therapy. In this study, we challenged germ-free and microbially colonized yolk sac fry Atlantic salmon with Flavobacterium columnare observed mere presence a protected fish against lethal infection. We then investigated effect phage- or oxytetracycline treatment on survival rearing water community characteristics using 16S rRNA gene amplicon sequencing. Phage led an increased F. columnare-challenged reduced relative amounts pathogen microbiota. absence columnare, phage did affect composition α-diversity phage's host, induced minor changes composition, without affecting α-diversity. Surprisingly, had no observable reduce abundance water. conclusion, showed prevents mortality while negatively microbiota, thus suggesting may be suitable antibiotics. also demonstrated protective fry.

Language: Английский

Citations

6
The stability and composition of the gut and skin microbiota of Atlantic salmon throughout the yolk sac stage DOI Creative Commons
Alexander W. Fiedler,

Martha K. R. Drågen,

Eirik Degré Lorentsen

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: July 6, 2023

The bacterial colonization of newly hatched fish is important for the larval development and health. Still, little known about ontogeny early microbiota fish. Here, we conducted two independent experiments with yolk sac fry Atlantic salmon that were (1) either reared conventionally, eggs as only source bacteria (egg-derived microbiota; EDM) or (2) germ-free re-colonized using lake water (lake-derived LDM). First, characterized gut skin at 6, 9, 13 weeks post hatching based on extracted RNA. In second experiment, exposed to high doses a pathogen commensal isolate sampled DNA. differed strongly between EDM LDM treatments. phyla Proteobacteria, Bacteroidetes, Actinobacteria dominated microbiota, which was found temporarily dynamic. Interestingly, more stable, both replicate rearing flasks, over time. Although similar, started differentiate during stage, several before consumed. Addition isolates flasks had minor effects microbiota.

Language: Английский

Citations

5
Feeding and water temperature modulate the potential activity of gut microbiota in greater amberjack juveniles (Seriola dumerili) DOI Creative Commons
Carmen Navarro-Guillén,

Eirik Degré Lorentsen,

Erick Perera

et al.

Frontiers in Marine Science, Journal Year: 2023, Volume and Issue: 10

Published: Sept. 12, 2023

Gut microbial communities are highly dynamic and respond to factors such as diet host metabolism. However, there is limited knowledge on changes in gut microbiota during the daily cycle. To investigate how interactions between feeding water temperature affected diurnal dynamics activity of microbiota, we analyzed qualitative quantitative composition feces potentially active bacterial community along a day cycle greater amberjack juveniles ( Seriola dumerili ). Bacterial density (based qPCR 16S rDNA) was relatively stable day. In contrast rRNA) increased period feeding, reduced pre-feeding levels four hours after last meal. The relative potential fish varied with postprandial time. At phylum level shift from Spirochaetes-dominated morning Proteobacteria feeding. lower taxonomic level, i.e. order, dominated by Mycoplasmoidales, which maintained throughout all treatments. By contrast, for absolute at order peak several orders 6h first most noticeable increase observed Mycoplasmoidales. Results present work also indicated close relation effect dynamics. This study assessing effects rhythm fish. Feeding main driver modulating activity, promoting modulated dynamics, marked time scale. addition, results indicate that analysis provides better resolution metabolically community, since change single taxon can alter taxa.

Language: Английский

Citations

5
Damage on intestinal barrier function and Microbial detoxification of deoxynivalenol: A review1 DOI Creative Commons
Jia Chen, Xinran Zhang, Ziqi He

et al.

Journal of Integrative Agriculture, Journal Year: 2023, Volume and Issue: 23(8), P. 2507 - 2524

Published: Nov. 28, 2023

Deoxynivalenol (DON) is a mycotoxin that produced by various species of Fusarium and ubiquitous in food feed. At low concentrations, it can cause metabolic disorders animals humans and, at high lead to pathological changes the body. The impact DON on human/animal health animal productivity has thus attracted great deal attention around world. causes severe damage intestine, including compromised intestinal barrier, mucosal damage, weakened immune function, alterations gut microbiota composition. These effects exacerbate infections inflammation livestock poultry, posing adverse overall health. Furthermore, research into biological methods for detoxification crucial avenue future studies. This includes utilization adsorption, enzymatic degradation, other approaches mitigate DON's impact, offering new strategies prevention treatment DON-induced diseases. Future will focus identifying highly efficient detoxifying microorganisms or enzymes reduce levels feed, thereby mitigating its risks both human

Language: Английский

Citations

2
Phage therapy minimally affects the water microbiota in an Atlantic salmon (Salmo salar) rearing system while still preventing infection DOI Creative Commons
Alexander W. Fiedler, Madeleine S. Gundersen,

Toan P. Vo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 4, 2023

Abstract Excessive usage of antibiotics threatens the bacterial diversity in microbiota animals. An alternative to that has been suggested not disturb is (bacterio)phage therapy. In this study, we challenged germ-free and microbially colonized yolk sac fry Atlantic salmon with Flavobacterium columnare observed mere presence a protected fish against lethal infection. We then investigated effect phage-or oxytetracycline treatment on survival rearing water community characteristics using 16S rRNA gene amplicon sequencing. Phage led an increased F. columnare- reduced relative amounts pathogen microbiota. absence , phage did affect composition or α-diversity phage’s host, induced minor changes composition, without affecting α-diversity. Surprisingly, had no observable reduce abundance water. conclusion, showed prevents mortality while negatively microbiota, thus suggesting may be suitable antibiotics. also demonstrated protective fry.

Language: Английский

Citations

0