Investigating the association between Candida albicans and early childhood dental caries: A comprehensive systematic review and meta-analysis DOI Creative Commons
Lusine Khachatryan,

Fatemeh Allahbakhsi,

Diana E. Vadiyan

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0315086 - e0315086

Published: Dec. 16, 2024

Background Early childhood caries (ECC) is a significant public health concern affecting children globally. Recent studies suggest potential association between the presence of Candida albicans (C . albicans) in oral cavity and risk ECC, but findings have been inconsistent. This systematic review meta-analysis aimed to investigate C ECC. Methods A comprehensive literature search was conducted across databases including PubMed, Web Science, Cochrane Central Register Controlled Trials, Embase, Google Scholar, Scopus. Studies were included if they examined occurrence or severity dental children. Data extraction quality assessment performed independently by two reviewers. Statistical analyses, pooled odds ratios (OR) 95% confidence intervals (CI), synthesize findings. Results total 22 studies, involving 3301 participants, meta-analysis. The revealed an increased likelihood with ratio 4.42 (95% CI: 3.14–6.24, p < 0.001). Subgroup analyses showed that factors such as geographic region, study design, detection methods influenced strength this association. Europe reported stronger (OR: 10.13, 6.01–17.09) compared those Asia 3.62, 2.53–5.20) Americas 3.98, 1.14–13.87). Case-control had higher OR (5.30, 1.72–16.31) cross-sectional (4.30, 2.92–6.33). ECC 4.08 2.65–6.27) plaque samples, 9.55 2.17–42.01) swab 4.70 2.44–9.09) saliva samples. No publication bias observed based on Begg’s test (P-value = 0.612) Egger’s 0.250). Conclusions provide support for hypothesis plays role development Moving forward, it essential future research concentrate comprehending mechanisms underlie relationship develop specific strategies prevention.

Language: Английский

Biofilm formation of Streptococcus mutans, Streptococcus sanguinis, Staphylococcus epidermidis, Staphylococcus aureus, Lactobacillus casei, and Candida Albicans on 5 thermoform and 3D printed orthodontic clear aligner and retainer materials at 3 time points: an in vitro study DOI Creative Commons

Mehrnaz Moradinezhad,

Effat Abbasi Montazeri, Alireza Hashemi Ashtiani

et al.

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Sept. 18, 2024

Orthodontic clear aligners and retainers have numerous advantages that is making them ever increasingly popular. However, they might, similar to any other oral appliance, contribute biofilm formation finally dental caries or white spot lesions gingival inflammations. The literature on orthodontic appliances very scarce limited a few microorganisms materials. Therefore, this experimental study evaluated the 5 thermoformed 3D printed CAD/CAM in 3 intervals.

Language: Английский

Citations

2
Polyalthic Acid from Copaifera lucens Demonstrates Anticariogenic and Antiparasitic Properties for Safe Use DOI Creative Commons
Mariana B. Santiago,

Vinícius Cristian Oti dos Santos,

Samuel Cota Teixeira

et al.

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(10), P. 1357 - 1357

Published: Sept. 26, 2023

This study aimed at evaluating the potential of Copaifera lucens, specifically its oleoresin (CLO), extract (CECL), and compound ent-polyalthic acid (PA), in combating caries toxoplasmosis, while also assessing toxicity. The involved multiple assessments, including determining minimum inhibitory concentration (MIC) bactericidal (MBC) against cariogenic bacteria. CLO PA exhibited MIC MBC values ranging from 25 to 50 μg/mL, whereas CECL showed equal or exceeding 400 μg/mL. displayed antibiofilm activity with biofilm (MICB50) spanning 62.5 1000 Moreover, effectively hindered intracellular proliferation Toxoplasma gondii 64 even after 24 h without treatment. Toxicological evaluations included vitro tests on V79 cells, where concentrations ranged 78.1 1250 μg/mL reduced colony formation. Additionally, using Caenorhabditis elegans model, lethal (LC50) was determined as 48 incubation. Notably, no significant differences micronucleus induction NDI were observed cultures treated 10, 20, 40 CLO. These findings underscore safety profile PA, highlighting their alternative treatments for toxoplasmosis.

Language: Английский

Citations

6
Fluoride export is required for competitive fitness of pathogenic microorganisms in dental biofilm models DOI Creative Commons
Aditya Banerjee, Chia-Yu Kang, Minjun An

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 18, 2024

Abstract Microorganisms resist fluoride toxicity using export proteins from one of several different molecular families. Cariogenic species Streptococcus mutans and Candida albicans extrude intracellular a CLC F − /H + antiporter FEX channel, respectively, whereas commensal eubacteria, such as gordonii, Fluc channel. In this work, we examine how genetic knockout impacts pathogen fitness in single-species three-species dental biofilm models. For biofilms generated S. with the transporter, exposure to low concentrations decreased counts, synergistically reduced populations C. , increased relative proportion gordonii properties associated pathogenicity, including acid production hydroxyapatite dissolution. Biofilms prepared channel also exhibited presence fluoride, but lesser degree. Imaging studies indicate that is highly sensitive strain undergoing complete lysis when exposed for moderate amount time, biochemical purification transporter functional reconstitution establishes protein dimer encoded by single gene. Together, these findings suggest oral pathogens can be targeted specific inhibitors restore symbiosis biofilms, especially susceptible toxicity. Importance Dental caries globally prevalent condition occurs pathogenic species, outcompete beneficial biofilm. Fluoride routinely used hygiene prevent caries. has antimicrobial properties, although most microbes possess exporters its This work shows sensitization cariogenic alters microbial composition biofilms. These results development drugs inhibit could potentiate anticaries effect over-the-counter products like toothpastes mouth rinses. novel strategy treat

Language: Английский

Citations

1
Measurement and analysis of microbial fluoride resistance in dental biofilm models DOI
Aditya Banerjee, Randy B Stockbridge, Lívia Maria Andaló Tenuta

et al.

Methods in enzymology on CD-ROM/Methods in enzymology, Journal Year: 2024, Volume and Issue: unknown, P. 155 - 174

Published: Jan. 1, 2024

Language: Английский

Citations

1
Fluoride-resistant Streptococcus mutans within cross-kingdom biofilms support Candida albicans growth under fluoride and attenuate the in vitro anti-caries effect of fluorine DOI Creative Commons

Yan Sun,

Yanhan Chen, Qian Du

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 5, 2024

Fluoride-resistant Streptococcus mutans ( S. ) might affect the ecological balance of biofilms in presence fluoride. We used a and Candida albicans C. cross-kingdom biofilm model to investigate whether fluoride-resistant would support growth under fluoride stress attenuate vitro anti-caries effect fluorine. The impact on formation by was investigated using crystal violet staining assay. Biofilm constitution determined colony-forming unit (CFU) counts fluorescent situ hybridization (FISH). Extracellular polysaccharide (EPS) generation EPS/bacterial dying water-insoluble detection. Acid production demineralization were monitored pH, lactic acid content, transversal microradiography (TMR). gene expression microorganisms measured qRT-PCR. Our results showed that both grew vigorously, forming robust biofilms, even sodium (NaF). Moreover, -containing had considerable cariogenic potential for EPS synthesis, production, ability NaF than fluoride-sensitive biofilms. Furthermore, two changed dissimilarly NaF. In summary, supported fluorine maintaining virulence

Language: Английский

Citations

1
Role of socioeconomic factors and interkingdom crosstalk in the dental plaque microbiome in early childhood caries DOI Creative Commons
Mohd Wasif Khan, Vivianne Cruz de Jesus,

Betty-Anne Mittermuller

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(8), P. 114635 - 114635

Published: Aug. 1, 2024

Early childhood caries (ECC) is influenced by microbial and host factors, including social, behavioral, oral health. In this cross-sectional study, we analyze interkingdom dynamics in the dental plaque microbiome its association with variables. We use 16S rRNA ITS1 amplicon sequencing on samples collected from preschool children questionnaire data to examine social determinants of The results indicate a significant enrichment Streptococcus mutans Candida dubliniensis ECC samples, contrast Neisseria oralis caries-free children. Our correlation analysis reveals that strongly correlated both bacilliformis Prevotella veroralis ECC. Additionally, shows associations variables, health status, age, place residence, mode childbirth. This study provides empirical evidence associating socioeconomic behavioral factors relation ECC, offering insights for developing targeted prevention strategies.

Language: Английский

Citations

1
Trans, Trans-Farnesol Enhances the Anti-Bacterial and Anti-Biofilm Effect of Arachidonic Acid on the Cariogenic Bacteria Streptococcus mutans and Streptococcus sobrinus DOI Open Access

Farah Haj-Yahya,

Doron Steinberg,

Ronit Vogt Sionov

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11770 - 11770

Published: Nov. 1, 2024

Background: Streptococcus mutans and sobrinus are Gram-positive bacteria involved in the development of dental caries, as they able to form biofilms on tooth enamel, ferment sugars into acids, survive under acidic conditions. This ultimately leads a local lowering pH value surface, which causes enamel cavities. Hypothesis: One measure reduce caries is limit growth cariogenic by using two anti-bacterial agents with different mechanisms action. The hypothesis this study was that activity ω-6 polyunsaturated arachidonic acid (AA) against S. can be enhanced sesquiterpene alcohol trans, trans-farnesol (t,t-farnesol). Methods: single combined treatment determined checkerboard assay. Bacterial viability assessed live/dead SYTO 9/propidium iodide (PI) staining flow cytometry. Anti-biofilm MTT metabolic assay, crystal violet biofilm biomass, 9/PI spinning disk confocal microscopy (SDCM) high-resolution scanning electron (HR-SEM). Results: t,t-Farnesol lowered minimum inhibitory concentration (MIC) (MBIC) AA at sub-MICs. reduced preformed mature biofilms, while t,t-farnesol had no significant effect. effect t,t-farnesol/AA further evidenced increased PI uptake, indicating membrane perforation. anti-biofilm verified SDCM HR-SEM. Gene expression studies showed some biofilm-related genes. Conclusions: Altogether, our suggests potential use naturally occurring compounds for preventing formation sobrinus. These findings have implications prevention.

Language: Английский

Citations

1
Cetyltrimethylammonium Chloride (CTAC) and Its Formulated Mouthwash Reduce the Infectivity of Streptococcus mutans and Candida albicans in Mono and Dual State DOI

Ravichellam Sangavi,

Ravi Jothi,

Nambiraman Malligarjunan

et al.

Applied Biochemistry and Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 28, 2024

Language: Английский

Citations

1
Role of socioeconomic factors and interkingdom crosstalk in the dental plaque microbiome in early childhood caries DOI Creative Commons
Mohd Wasif Khan, Vivianne Cruz de Jesus,

Betty-Anne Mittermuller

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 18, 2024

SUMMARY Early childhood caries (ECC) is influenced by microbial and host factors, including social, behavioral, oral health. In this cross-sectional study, we analyzed interkingdom dynamics in the dental plaque microbiome its association with variables. The samples collected from preschool children underwent 16S rRNA ITS1 gene sequencing. questionnaire data were for social determinants of results indicated a significant enrichment Streptococcus mutans Candida dubliniensis ECC samples, contrast to Neisseria oralis caries-free children. Our correlation analysis revealed that was strongly correlated both bacilliformis Prevotella veroralis ECC. Additionally, showed associations variables, health status, age, place residence, mode childbirth. This study provides empirical evidence associating socioeconomic behavioral factors relation ECC, offering insights developing targeted prevention strategies. HIGHLIGHTS Characterized between cariogenic species genus Both bacterial fungal are important status prediction using artificial intelligence Socioeconomic index associated caries-associated markers

Language: Английский

Citations

0
Dietary Immunoglobulin Y by Targeting Both GbpB and GtfB Enhances the Anticaries Effect in Rats DOI Creative Commons

Yunxiao Du,

Guobin Li, Xinglin Li

et al.

International Dental Journal, Journal Year: 2024, Volume and Issue: 74(6), P. 1298 - 1305

Published: May 25, 2024

The purpose of this work was to develop an anti-CAT-SYI immunoglobulin Y (IgY) antibody that targeted both GtfB (glucosyltransferase B) and GbpB (glucan-binding protein test its anticaries properties in rats.

Language: Английский

Citations

0