The Microbiological Landscape of Diabetic Foot Infections: Implications for Treatment and Prevention DOI Open Access
Reem A. Mansour,

Doaa A. Abdulwaha

International Journal of Medical Science and Dental Health, Journal Year: 2024, Volume and Issue: 10(02), P. 65 - 85

Published: Feb. 15, 2024

Diabetic foot infections (DFIs) represent one of the most serious and frequent complications resulting from diabetes mellitus, causing substantial morbidity as well enhanced risk for amputation. The microbiological world DIFs is typically multifaceted usually includes a polymicrobial community with both aerobic bacteria this anaerobe purpose review to decompose current knowledge about microbial diversity in DFIs, their resistance patterns biofilms contribution resilience towards infection persistence therapy. Development molecular techniques has allowed increased insight into DFI microbiome, not only uncovering prevalent pathogens such Staphylococcus aureus Pseudomonas aeruginosa but also lesser frequently isolated organisms. employment multidrug-resistant organisms intricate relationship between species within present management challenges. focuses on relevance early accurate diagnosis using culture-based methodologies methods that, turn, would enable more specific antimicrobial Moreover, opportunity new antibacterial agents will be available; other local delivery systems adjunctive therapies like negative pressure wound therapy addressed. Strategies prevention that involve patient education, regular checks proper glycemic control form part essential measures reducing incidences DFIs. An interdisciplinary approach care highlighted review, influencing diabetology specialists, infectious disease physicians microbiologists podiatrists able improve treatment outcomes. This aims provide recent synthesis aspects DFIs clinical implications, an intent informing development effective strategies thus improving patient’s quality life health systems.

Language: Английский

Periodontitis: etiology, conventional treatments, and emerging bacteriophage and predatory bacteria therapies DOI Creative Commons
Anna M. Łasica, Piotr Golec,

Agnieszka Laskus

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 26, 2024

Inflammatory periodontal diseases associated with the accumulation of dental biofilm, such as gingivitis and periodontitis, are very common pose clinical problems for clinicians patients. Gingivitis is a mild form gum disease when treated quickly properly completely reversible. Periodontitis an advanced irreversible periodontium periods exacerbations, progressions remission. chronic inflammatory condition that damages tissues supporting tooth in its socket, i.e., gums, ligaments, root cementum bone. Periodontal inflammation most commonly triggered by bacteria present excessive accumulations plaque (biofilm) on surfaces. This driven disproportionate host immune responses induced imbalance composition oral changes their metabolic activities. microbial dysbiosis favors establishment conditions ultimately results destruction tooth-supporting tissues. Apart shift response, environmental factors genetics also important etiology In addition to destruction, can result significant systemic complications. Conventional methods treatment (improving hygiene, biofilm control, mechanical removal, using local or antimicrobial agents) not fully effective. All this prompts search new therapy. Advanced periodontitis multiple abscesses often antibiotics, amoxicillin, tetracycline, doxycycline, minocycline, clindamycin, combined therapy amoxicillin metronidazole. However, due growing problem antibiotic resistance, does always achieve desired therapeutic effect. review summarizes pathogenesis, current approaches treatment, limitations state research possibility application bacteriophages predatory combat responsible periodontitis. We landscape potential applications alternative therapies based phages bacteria, highlight gaps existing knowledge need be addressed before trials utilizing these strategies seriously considered.

Language: Английский

Citations

16
Green Synthesis of Silver-Chitosan Nanocomposite Exhibits Promising Antibiofilm Properties Against Pathogenic Bacteria Escherichia coli and Staphylococcus aureus DOI Creative Commons

Subhransu Sekhar Behera,

Amrito Ghosh Majumdar,

Himansu Gullani

et al.

The Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 100264 - 100264

Published: Feb. 1, 2025

Language: Английский

Citations

2
Current strategies against multidrug-resistant Staphylococcus aureus and advances toward future therapy DOI Creative Commons
Saurabh Kumar, Richa Prasad Mahato,

Sanjay Ch

et al.

The Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 100281 - 100281

Published: Feb. 1, 2025

Language: Английский

Citations

1
Advancements in Antibacterial Therapy: Feature Papers DOI Creative Commons
Giancarlo Angeles Flores,

Gaia Cusumano,

Roberto Venanzoni

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 557 - 557

Published: March 1, 2025

Antimicrobial resistance (AMR) is a growing global health crisis that threatens the efficacy of antibiotics and modern medical interventions. The emergence multidrug-resistant (MDR) pathogens, exacerbated by misuse in healthcare agriculture, underscores urgent need for innovative solutions. (1) Background: AMR arises from complex interactions between human, animal, environmental health, further aggravated overuse inadequate regulation antibiotics. Conventional treatments are increasingly ineffective, necessitating alternative strategies. Emerging approaches, including bacteriophage therapy, antimicrobial peptides (AMPs), nanotechnology, microbial extracellular vesicles (EVs), CRISPR-based antimicrobials, provide novel mechanisms complement traditional combating resistant pathogens. (2) Methods: This review critically analyzes advanced antibacterial strategies conjunction with systemic reforms such as stewardship programs, One Health framework, surveillance tools. These methods can enhance detection, guide interventions, promote sustainable practices. Additionally, economic, logistical, regulatory challenges impeding their implementation evaluated. (3) Results: technologies, CRISPR exhibit promising potential targeting mechanisms. However, disparities resource distribution barriers hinder widespread adoption. Public–private partnerships agriculture practices critical to overcoming these obstacles. (4) Conclusions: A holistic integrated approach essential mitigating impact AMR. By aligning therapeutic policies, fostering interdisciplinary collaboration, ensuring equitable distribution, we develop response this 21st-century challenge.

Language: Английский

Citations

1
A Klebsiella-phage cocktail to broaden the host range and delay bacteriophage resistance both in vitro and in vivo DOI Creative Commons

Huanchang Chen,

Haifeng Liu, Yanchun Gong

et al.

npj Biofilms and Microbiomes, Journal Year: 2024, Volume and Issue: 10(1)

Published: Nov. 14, 2024

Bacteriophages (phages), viruses capable of infecting and lysing bacteria, are a promising alternative for treating infections from hypervirulent, antibiotic-resistant pathogens like Klebsiella pneumoniae, though narrow host range phage resistance remain challenges. In this study, the hypervirulent K. pneumoniae NTUH-K2044 was used to purify ΦK2044, while two ΦK2044-resistant strains were further phages: ΦKR1, ΦKR8 hospital sewage. A detailed characterization showed that ΦK2044 specifically killed KL1 capsule-type ΦKR1 targeted 13 different capsular serotypes. The cocktail (ΦK2044 + ΦKR8) effectively in biofilms, pre-treatment biofilm formation, delayed phage-resistance. improved 7-day survival Galleria mellonella mouse models therapeutic potential catheter model. summary, proof-of-principle has broad range, including highly drug-resistant serves as starting point optimizing therapy.

Language: Английский

Citations

7
Antibacterial, antibiofilm and anti-quorum sensing activities of 1,2,3,5-tetrazine derivatives linked to a benzothiazole moiety DOI Creative Commons
Jean Paul Dzoyem, Joseph Tsemeugne, Boniface Pone Kamdem

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Abstract A series of known tetrazine derivatives, containing benzothiazole scaffold, were prepared during the coupling reactions selected diazotized 2-aminobenzo[d]thiazole derivatives with p-acetaminophen. The as-prepared compounds characterized based on NMR and MS spectrometry. antibacterial anti-biofilm activities synthesized evaluated by microdilution method, whereas anti-quorum sensing effect was carried out using assay for inhibition violacein formation. As a result, 4a, 4b 4c revealed minimum inhibitory concentrations bactericidal ranging from 8 to 128 µg/mL 32 256 µg/mL, respectively. Compounds 4a (52-86.5%), (57.7-79.4%) (59.9-80.3%) prevented biofilm formation in all four bacteria tested percentages more than 50%. that inhibited 50% production found be 62.71, 28.56 107.45 respectively, thus attesting these possess activity. Noteworthy, our previous investigation attested are non-cytotoxic human mammalian cells Vero. This novel contribution demonstrates antibacterial, antibiofilm tetrazine-based benzothiazoles, which might prospected as scaffolds discovery efficient antibiotics decreased risk microbial drug resistance.

Language: Английский

Citations

0
Indicators of the Microbial Corrosion of Steel Induced by Sulfate-Reducing Bacteria Under the Influence of Certain Drugs DOI Creative Commons
Наталія Ткачук, Любов Зелена,

Yaroslav Novikov

et al.

Microbiology Research, Journal Year: 2025, Volume and Issue: 16(1), P. 21 - 21

Published: Jan. 16, 2025

Microorganisms cause microbiologically influenced corrosion, for the prevention of which bactericide inhibitors are used. The aim work was to study in vitro sensitivity SRB Desulfovibrio oryzae NUChC SRB1 different concentrations dimethyl sulfoxide (DMSO), and evaluate indicators microbial corrosion steel induced by this bacterium presence pharmaceutical drugs DMSO paracetamol. D. 1–100% (v/v) studied via dilution method Postgate’s “C” liquid medium. activity against 3 investigated under paracetamol treatment at a final concentration 45% 0.2% (w/v), respectively, according ability bacteria form biofilm on surface samples (via crystal violet method) effect rate gravimetric method). It revealed that affected exhibited bactericidal properties (at range 10–100%, v/v) antibiofilm 45%, v/v). Despite its confirmed reduction bacterial mass, anticorrosion features were not observed model 35-day conditions an anaerobic environment with sulfate reduction. Paracetamol (0.2%, w/v) did affect formation these conditions, significantly contributed increase steel. prospect further research is assess influence compounds found wastewater, clarify possible additional causes damage equipment plants. Further should also be directed investigating antimicrobial complexes DMSO, considered as ecological solution problem prevention.

Language: Английский

Citations

0
Prevalence, Antibiotic Resistance Patterns, and Virulence Factors of Staphylococcus aureus Isolates Associated with Bovine Mastitis in Northern Bangladesh DOI Creative Commons
Md Mominul Islam, Md Imran Hossain,

Md. Sadequl Islam

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(3), P. e42107 - e42107

Published: Jan. 21, 2025

Staphylococcus (S.) aureus is a major cause of bovine mastitis and notorious for its capacity to resist antibiotics, presenting substantial risks both livestock human health. The aim this research was assess the prevalence S. in cases, as well examine their patterns antimicrobial resistance virulence genes contributing cattle. For study, 120 milk samples were gathered from clinically affected cows across three districts northern part Bangladesh. detection confirmation involved standard microbiological biochemical techniques. antibiotic sensitivity strains evaluated using disk diffusion method with variety antibiotics frequently used veterinary settings. Furthermore, PCR utilized explore presence linked pathogenicity aureus. Findings revealed that out sampled 56 isolated, indicating rate 46.66 % cases clinical mastitis. results diverse range among isolates, notable penicillin (100 %), ampicillin (85 amoxicillin (75 tetracycline (66 chlortetracycline (64 azithromycin (57 kanamycin (54 gentamicin (50 %). 36 isolates %) multidrug resistant nature. gene profiling identified responsible biofilm formation (bap), adhesion, inflammation tissue damage (seb, pvl), toxin production (hla hlb), potential isolates. Notably, 12 (21.42 harbored methicillin (mecA), raising concerns about transmission antimicrobial-resistant dairy humans through food chain. These findings underscore critical importance implementing stringent stewardship practices surveillance measures farming mitigate dissemination resistance.

Language: Английский

Citations

0
Electrogenic Dynamics of Biofilm Formation: Correlation Between Genetic Expression and Electrochemical Activity in Bacillus subtilis DOI Creative Commons
Adel Yavarinasab, Jerry He, Abhirup Mookherjee

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117218 - 117218

Published: Jan. 1, 2025

Language: Английский

Citations

0
Frontiers in superbug management: innovating approaches to combat antimicrobial resistance DOI
Priyanka Chambial, Neelam Thakur, Prudhvi Lal Bhukya

et al.

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(3)

Published: Feb. 14, 2025

Language: Английский

Citations

0