miR-21-loaded bone marrow mesenchymal stem cell-derived exosomes inhibit pyroptosis by targeting MALT1 to repair chemotherapy-induced premature ovarian insufficiency DOI Creative Commons

Lichao Tang,

Yutao Yang,

Mingxin Yang

et al.

Cell Biology and Toxicology, Journal Year: 2024, Volume and Issue: 41(1)

Published: Dec. 20, 2024

Chemotherapy is essential for treating malignant tumors, but it can cause premature ovarian insufficiency (POI). Recent studies suggest that exosomes enriched with miR-21 (miR-21-Exo) may help mitigate POI, though the underlying mechanisms remain largely unexplored. This research investigates how miR-21-Exo influences chemotherapy-induced POI using an experimental model where KGN cells are exposed to cisplatin. We assessed impact of on cellular activity and generated overexpressing bone marrow mesenchymal stem (miR-21-BMSC) via lentiviral modification. Isolated was analyzed its effects function. Bioinformatics identified Mucosa-Associated Lymphoid Tissue Lymphoma Translocation Protein 1 (MALT1) as a target miR-21. confirmed regulates MALT1 NF-κB signaling pathway prevent cell pyroptosis. Further in rat demonstrated therapeutic potential safety miR-21-Exo. Overall, our findings highlight novel strategy addressing by modulating pathway, offering significant implications. 1. Successful construction validation miR-21-loaded cell-derived (miR-21-Exo). 2. facilitates proliferation granulosa cells, suppresses apoptosis pyroptosis, increases secretion estrogen hormones vitro vivo. 3. regulate inhibits pyroptosis cells.

Language: Английский

Premature ovarian insufficiency: a review on the role of oxidative stress and the application of antioxidants DOI Creative Commons

Yuqian Shi,

Xi-Ting Zhu,

Zhang Su-na

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 1, 2023

Normal levels of reactive oxygen species (ROS) play an important role in regulating follicular growth, angiogenesis and sex hormone synthesis ovarian tissue. When the balance between ROS antioxidants is disrupted, however, it can cause serious consequences oxidative stress (OS), quantity quality oocytes will decline. Therefore, this review discusses interrelationship OS premature insufficiency (POI), potential mechanisms methods by which improve POI through controlling level OS. We found that mediate changes genetic materials, signal pathways, transcription factors microenvironment, resulting abnormal apoptosis granulosa cells (GCs) meiosis as well decreased mitochondrial Deoxyribonucleic Acid(mtDNA) other changes, thus accelerating process aging. However, antioxidants, mesenchymal stem (MSCs), biological enzymes delay disease reducing vivo .

Language: Английский

Citations

50
Targeting autophagy in premature ovarian failure: Therapeutic strategies from molecular pathways to clinical applications DOI

Ziwen Ding,

Genbao Shao, Mingyang Li

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123473 - 123473

Published: Feb. 1, 2025

Language: Английский

Citations

1
Inducers and Inhibitors of Pyroptotic Death of Granulosa Cells in Models of Premature Ovarian Insufficiency and Polycystic Ovary Syndrome DOI Creative Commons
Çağlar Berkel

Reproductive Sciences, Journal Year: 2024, Volume and Issue: 31(10), P. 2972 - 2992

Published: July 18, 2024

Abstract Granulosa cells (GCs), the largest cell population and primary source of steroid hormones in ovary, are important somatic ovarian components. They have critical roles folliculogenesis by supporting oocyte, facilitating its growth, providing a microenvironment suitable for follicular development oocyte maturation, thus having essential functions maintaining female fertility reproductive health general. Pyroptotic death GCs associated inflammation been implicated pathogenesis several disorders females including Premature Ovarian Insufficiency (POI) Polycystic Ovary Syndrome (PCOS). Here, I reviewed factors, either intrinsic or extrinsic, that induce inhibit pyroptosis various models these disorders, both vitro vivo, also covered molecular mechanisms. Most studied factors influence NLRP3 inflammasome- GSDMD (Gasdermin D)-mediated GCs, compared to other inflammasomes gasdermins (GSDMs). conclude more complete mechanistic understanding terms GC is required be able develop novel strategies targeting inflammatory ovary.

Language: Английский

Citations

5
TCM “medicine and food homology” in the management of post-COVID disorders DOI Creative Commons
C. Y. Ng,

H. Bun,

Yan Zhao

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 31, 2023

The World Health Organization declared that COVID-19 is no longer a public health emergency of global concern on May 5, 2023. Post-COVID disorders are, however, becoming more common. Hence, there lies growing need to develop safe and effective treatment measures manage post-COVID disorders. Investigating the use TCM medicinal foods in long-term therapy illnesses may be beneficial given contemporary research's emphasis development foods.The for highlighted this review. Following discussion history "Medicine Food Homology" theory, pathophysiological effects will briefly reviewed. An analysis their functions treating then provided before offering some insight into potential directions future research application.TCM can different aspects management efficient choice because they are typically milder nature than chronic drug use. These findings also applied post-disease similar respiratory

Language: Английский

Citations

13
Oxidative stress and antioxidant therapy in the treatment of premature ovarian insufficiency and improving the quality of life DOI
L.V. Adamyan, Anna Osipova, L.G. Pivazyan

et al.

Russian Journal of Human Reproduction, Journal Year: 2025, Volume and Issue: 31(1), P. 21 - 21

Published: March 12, 2025

Reactive oxygen species (ROS), at physiological concentrations, play a key role in regulating follicular growth, angiogenesis, and the biosynthesis of sex steroids ovarian tissue. An imbalance between ROS body’s antioxidant system leads to oxidative stress (OS), which results degradation both quantitative qualitative characteristics oocytes. This review analyzes relationship OS premature insufficiency (POI), highlights potential pathogenic mechanisms, explores treatment approaches for POI through modulation levels via therapy. It has been established that plays significant genetic material damage, disrupts signaling pathways, transcription factor expression, alters oocyte microenvironment, ultimately leading apoptosis granulosa cells (GCs), disruption meiotic division, reduction mitochondrial DNA (mtDNA) content, other pathophysiological changes accelerate aging. Antioxidants, mesenchymal stem (MSCs), biological enzymes, agents have slow down progression by reducing representing promising direction improving quality life reproductive outcomes patients with POI.

Language: Английский

Citations

0
Cistanche Deserticola Polysaccharides Protect Against Cyclophosphamide-induced Premature Ovarian Failure in Mice by Regulating the JAK-STAT Pathway DOI
Jinhua Li, Weiwei Sun, Xing Wan

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119971 - 119971

Published: May 1, 2025

Language: Английский

Citations

0
Fluoride promotes the secretion of inflammatory factors in microglia through NLRP3/Caspase-1/GSDMD pathway DOI
Qiuyi Zhang, Tao Li, Ruili Shi

et al.

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(13), P. 19844 - 19855

Published: Feb. 17, 2024

Language: Английский

Citations

2
Advances in cytokine-based herbal medicine against premature ovarian insufficiency: A review DOI
Siyu Yuan,

Zhu Shixiao,

Sun Congying

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 333, P. 118477 - 118477

Published: June 21, 2024

Language: Английский

Citations

2
Mitochondrial Quality Control in Ovarian Function: From Mechanisms to Therapeutic Strategies DOI
Xiaomei Wang, Yuxin Liu, Jinzheng Wang

et al.

Reproductive Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: July 9, 2024

Language: Английский

Citations

2
Proanthocyanidins protects 3-NPA-induced ovarian function decline by activating SESTRIN2-NRF2-mediated oxidative stress in mice DOI Creative Commons

Yupei Huang,

Yanfan Cui,

Jian Huang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 27, 2024

Abstract Abnormal apoptosis of ovarian cells caused by oxidative stress is an important cause premature failure (POF). Previous studies revealed that proanthocyanidins (PCs) are powerful natural antioxidants can safely prevent damage in humans. However, the protective effect and mechanism PCs on function during course POF remain unknown. In this study, female mice were injected with 3-nitropropionic acid (3-NPA) to establish model; at same time, treated PC via gavage. Thereafter, expression various genes, hormones, related molecules was assessed. Compared those control group, index, follicle count all levels, MVH, PCNA BCL2, estradiol (E2) progesterone (P) levels significantly lower but significant recovery observed terms MVH E2 P + group. The marker genes BAX ROS increased group notably restored addition, Sestrin2, antiapoptotic protein, treatment as upstream downstream regulatory factors NRF2 SOD2, indices Sestrin2 overexpression similar summary, these findings suggest have potential innovative therapeutic agents for preventing treating activating SESTRIN2-NRF2 pathway against stress.

Language: Английский

Citations

2