Stem Cell Reviews and Reports, Journal Year: 2023, Volume and Issue: 19(7), P. 2166 - 2191
Published: July 26, 2023
Language: Английский
Journal of Clinical Investigation, Journal Year: 2023, Volume and Issue: 133(19)
Published: Oct. 1, 2023
Alveolar macrophages (AMs) are the sentinel cells of alveolar space, maintaining homeostasis, fending off pathogens, and controlling lung inflammation. During acute injury, AMs orchestrate initiation resolution inflammation in order to ultimately restore homeostasis. This central role makes attractive targets for therapeutic interventions. Single-cell RNA-Seq spatial omics approaches, together with methodological advances such as generation human from pluripotent stem cells, have increased understanding ontogeny, function, plasticity during infectious sterile inflammation, which could move field closer clinical application. However, proresolution phenotypes might conflict proinflammatory antibacterial responses. Therefore, targeting at vulnerable time points over course injury harbor risk serious side effects, loss host defense capacity. Thus, identification key signaling hubs that determine functional fate decisions is utmost importance harness their potential.
Language: Английский
Citations
54
Respiratory Research, Journal Year: 2024, Volume and Issue: 25(1)
Published: Jan. 13, 2024
Abstract Acute respiratory distress syndrome (ARDS) is a common condition associated with critically ill patients, characterized by bilateral chest radiographical opacities refractory hypoxemia due to noncardiogenic pulmonary edema. Despite significant advances, the mortality of ARDS remains unacceptably high, and there are still no effective targeted pharmacotherapeutic agents. With outbreak coronavirus disease 19 worldwide, has increased correspondingly. Comprehending pathophysiology underlying molecular mechanisms may thus be essential developing therapeutic strategies reducing mortality. To facilitate further understanding its pathogenesis exploring novel therapeutics, this review provides comprehensive information from presents therapeutics. We first describe that involve dysregulated inflammation, alveolar-capillary barrier dysfunction, impaired alveolar fluid clearance oxidative stress. Next, we summarize signaling pathways related above four aspects pathophysiology, along latest research progress. Finally, discuss emerging show exciting promise in ARDS, including several pharmacologic therapies, microRNA-based therapies mesenchymal stromal cell highlighting pathophysiological basis influences on signal transduction for their use.
Language: Английский
Citations
20
Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13
Published: Nov. 17, 2022
Background Chronic rhinosinusitis with nasal polyps (CRSwNP) is a common sinonasal inflammatory disorder high heterogeneity. Increasing evidence have indicated that the infiltration of macrophages especially M2 play pivotal roles in pathogenesis CRSwNP, but underlying mechanisms remain undetermined. This study sought to identify potential biomarkers related CRSwNP. Methods The expression datasets GSE136825 and GSE179265 were download from Gene Expression Omnibus (GEO) database merged. Then, CIBERSORT weighted gene co-expression network analysis (WGCNA) algorithms applied macrophage-related modules. Thereafter, differentially expressed genes (DEGs) selected perform functional enrichment analyses. A protein-protein interaction (PPI) was built hub quantitative real-time reverse transcriptions PCR used verify bioinformatics results. Results total 92 DEGs associated identified for further analysis. results ontology (GO) Kyoto Encyclopedia genomes (KEGG) analyses illustrated macrophage-associated primarily enriched immune responses extracellular matrix structure. PPI 18 might be After verification, AIF1, C1QA, C1QB, C3AR1, CCR1, CD163, CD4, CD53, CD86, CSF1R, CYBB, FCER1G, FCGR3A, IL10RA, ITGB2, LAPTM5, PLEK, TYROBP as Conclusion These findings yield new insights into Further studies these would help better understand disease progression treatment targets.
Language: Английский
Citations
27
Phytomedicine, Journal Year: 2022, Volume and Issue: 108, P. 154489 - 154489
Published: Oct. 12, 2022
Language: Английский
Citations
24
Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11
Published: Jan. 17, 2023
Stem cell-based therapy has drawn attention as an alternative option for promoting prosthetic osteointegration in osteoporosis by virtue of its unique characteristics. However, estrogen deficiency is the main mechanism postmenopausal osteoporosis. Estrogen, effective antioxidant, deficienncy also results accumulation reactive oxygen species (ROS) body, affecting osteogenic differentiation stem cells and bone formation i In this study, we prepared a ROS-scavenging hydrogel crosslinking epigallocatechin-3-gallate (EGCG), 3-acrylamido phenylboronic acid (APBA) acrylamide. The engineered can scavenge ROS efficiently, enabling it to be cell carrier marrow-derived mesenchymal (BMSCs) protect delivered from ROS-mediated death osteogenesis inhibition, favorably enhancing tissue repair potential cells. Further
Language: Английский
Citations
15
Stem Cell Research & Therapy, Journal Year: 2023, Volume and Issue: 14(1)
Published: Nov. 14, 2023
Abstract Background Acute compartment syndrome (ACS) is one of the most common complications musculoskeletal injury, leading to necrosis and demise skeletal muscle cells. Our previous study showed that embryonic stem cells-derived mesenchymal cells (ESC–MSCs) are novel therapeutics in ACS treatment. As extracellular vesicles (EVs) rapidly gaining attention as cell-free have advantages over parental cells, therapeutic potential mechanisms EVs from ESC–MSCs on need be explored. Method In present study, we examined protective effects experimental rat model investigated role macrophages mediating these effects. Next, used transcriptome sequencing explore by which ESC–MSC-EVs regulate macrophage polarization. Furthermore, miRNA was performed identify candidates associated with Results We found intravenous administration ESC–MSC-EVs, given at time fasciotomy, significantly promotes anti-inflammation process, angiogenesis, functional recovery ACS. The beneficial were affecting polarization delivering various miRNAs NF-κB, JAK/STAT, PI3K/AKT pathways. data further illustrate mainly modulate via miR-21/PTEN, miR-320a/PTEN, miR-423/NLRP3, miR-100/mTOR, miR-26a/TLR3 axes. Conclusion Together, our results demonstrated ACS, wherein macrophages.
Language: Английский
Citations
15
Biomolecules, Journal Year: 2023, Volume and Issue: 13(3), P. 536 - 536
Published: March 15, 2023
Macrophages are innate immune cells and often classified as M1 macrophages (pro-inflammatory states) M2 (anti-inflammatory states). Exosomes cell-derived nanovesicles that range in diameter from 30 to 150 nm. Non-coding RNAs (ncRNAs), including microRNAs (miRNAs), long noncoding (lncRNAs), circular (circRNAs), abundant exosomes exosomal ncRNAs influence responses. Exosomal control macrophage-linked intercellular communication via their targets or signaling pathways, which can play positive negative roles lung cancer inflammatory disorders, acute injury (ALI), asthma, pulmonary fibrosis. In cancer, mediated between tumor tumor-associated (TAMs), coordinating proliferation, migration, invasion, metastasis, evasion, therapy resistance. illnesses, mediate macrophage activation inflammation promote inhibit damage. Furthermore, we also discussed the possible applications of ncRNA-based therapies for disorders.
Language: Английский
Citations
14
Inflammation Research, Journal Year: 2023, Volume and Issue: 72(6), P. 1237 - 1255
Published: May 22, 2023
Evidence indicated that the early stage transition of macrophages' polarization stages yielded a superior prognosis for acute lung injury (ALI) or respiratory distress syndrome (ARDS). Rhein (cassic acid) is one major component many traditional Chinese medicines, and has been reported to perform with strong anti-inflammation capabilities. However, role rhein played mechanism via which it did so in LPS-induced ALI/ARDS remain unclear.ALI/ARDS was induced by LPS (3 mg/kg, i.n, st), accompanied applications (50 100 i.p, qd), vehicle NFATc1 inhibitor (10 qd) vivo. Mice were sacrificed 48 h after modeling. Lung parameters, epithelial cell apoptosis, macrophage polarization, oxidative stress examined. In vitro, conditioned medium from alveolar cells stimulated used culturing RAW264.7 line, along administrations (5 25 μM). RNA sequencing, molecule docking, biotin pull-down, ChIP-qPCR, dual luciferase assay performed clarify mechanisms this pathological process.Rhein significantly attenuated tissue inflammation promoted M2 ALI/ARDS. alleviated intracellular ROS level, activation P65, thus M1 macrophages. terms mechanism, its protective roles targeting NFATc1/Trem2 axis, whose function mitigated both Trem2 blocking experiments.Rhein axis regulate response ALI/ARDS, shed more light on possibilities clinical treatments process.
Language: Английский
Citations
13
Stem Cell Reviews and Reports, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 8, 2025
Language: Английский
Citations
0
Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 17, 2025
Language: Английский
Citations
0