Zinc Oxide Nanoparticles as Potential Delivery Carrier: Green Synthesis by Aspergillus niger Endophytic Fungus, Characterization, and In Vitro/In Vivo Antibacterial Activity

Pharmaceuticals, Journal Year: 2022, Volume and Issue: 15(9), P. 1057 - 1057

Published: Aug. 26, 2022

We aimed to synthesize zinc oxide nanoparticles (ZnO NPs) using the endophytic fungal extract of Aspergillus niger. The prepared ZnO NPs were characterized, and their in vitro vivo antibacterial activity was investigated. Isolated fungus identification carried out 18S rRNA. A. niger employed for green synthesis NPs. elucidated against Staphylococcus aureus broth microdilution method quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, a systemic infection model mice. biosynthesized showed maximum optical density at 380 nm with characteristic peaks on Fourier-transform infrared spectrum. X-ray diffraction pattern highly matched standard platform crystals. Energy-dispersive analysis confirmed that main composition oxygen atoms. Scanning transmission electron microscopies spherical geometry smooth surface. Zeta potential measurements (26.6 ± 0.56 mV) verified adequate stability Minimum inhibitory concentrations S. isolates ranged from 8 128 µg/mL. revealed antibiofilm activity, resulting downregulation tested biofilm genes 29.17% isolates. Regarding experiment, reduced congestion fibrosis liver spleen tissues. They also improved function, increased survival rate, significantly decreased inflammatory markers (p < 0.05). synthesized by promising action

Language: Английский

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Next-generation probiotics: the upcoming biotherapeutics

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: April 15, 2024

Abstract Recent and continuing advances in gut microbiome research have pointed out the role of microbiota as an unexplored source potentially beneficial probiotic microbes. Along lines these advances, both public awareness acceptance probiotics are increasing. That’s why; academic industrial is dedicated to identifying investigating new microbial strains for development next-generation (NGPs). At this time, there a growing interest NGPs biotherapeutics that alter affect various diseases development. In work, we focused on some emergent promising NGPs, specifically Eubacterium hallii, Faecalibacterium prausnitzii, Roseburia spp., Akkermansia muciniphila , Bacteroides fragilis their presence can impact diseases. Emerging studies point roles open up novel therapeutic options. Interestingly, were found enhance gastrointestinal immunity, immunotherapy efficacy cancer patients, retain intestinal barrier integrity, generate valuable metabolites, especially short-chain fatty acids, decrease complications chemotherapy radiotherapy. Although many considered prevention treatment several chronic diseases, humans still lacking. Therefore, approval microbes from regulatory agencies rare. Besides, issues limit wide use market, such suitable methods culture storage oxygen-sensitive The present review goes over main points related gives viewpoint key hinder application. Furthermore, advancement human healthiness investigations by clarifying limitations traditional microorganisms, discussing characteristics emerging defining management certain ailments. Future should emphasize isolation, mechanisms action probiotics, safety, clinical humans.

Language: Английский

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Role of ketogenic diet in neurodegenerative diseases focusing on Alzheimer diseases: The guardian angle

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 95, P. 102233 - 102233

Published: Feb. 14, 2024

Language: Английский

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Traditional herbs against COVID-19: back to old weapons to combat the new pandemic

European journal of medical research, Journal Year: 2022, Volume and Issue: 27(1)

Published: Sept. 26, 2022

Abstract Background Recently, the coronavirus (COVID-19) pandemic is a chief public health disaster caused by severe acute respiratory syndrome 2 (SARS-CoV-2). There are no established effective preventive or therapeutic anti-COVID-19 drugs available except for some recently approved vaccines. Still, countless recent studies recommend various alternative and complementary approaches against COVID-19, which medicinal herbs employed as traditional remedies to enhance immunity struggle with viral infections. In addition, physicians worldwide highly interested in vitamin mineral supplements help them combat COVID-19 either through protection treatment. Dietary specifically D, C, zinc provide good prophylactic support presently treatment regimens. present work, we have focused on plant-based promising activities. Aim To enable investigators researchers identify potential herbal compounds anti-COVID activity be used therapies this pandemic. Main body This review highlights published concerning natural herbs, bioactive metabolites, dietary supplements, functional foods that could prevent and/or treat COVID-19. Herein, explored inhibitors of SARS-CoV-2 discussed how these form larger discussions diet disease. Moreover, investigating components, outlined several can fight hindering replication entry its host cells, deterring cytokine storm, other means. Finally, summarized products, foods, potent inhibit disease progression. Conclusions Based reviewed it was concluded doubt phytochemical components starring role deterrence cure contagion.

Language: Английский

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Anti-Biofilm and Antibacterial Activities of Cycas media R. Br Secondary Metabolites: In Silico, In Vitro, and In Vivo Approaches

Antibiotics, Journal Year: 2022, Volume and Issue: 11(8), P. 993 - 993

Published: July 24, 2022

Enterococcus species possess many virulence factors that have an essential role in exacerbating the infections caused by them. The current study aimed to evaluate effect of secondary metabolites ginkgetin (GINK) and sotetsuflavone (SOTE), isolated from Cycas media R. Br dichloromethane fraction, on faecalis (E. faecalis) isolates for first time. antibacterial antivirulence activities compounds were investigated using docking studies vitro determination minimum inhibitory concentrations (MICs). Additionally, flow cytometry scanning electron microscope (SEM) utilized assess SOTE tested bacteria. Moreover, crystal violet assay qRT-PCR used test biofilm-forming ability E. isolates. In addition, a systemic infection model was vivo investigate activity SOTE. We found both GINK showed good affinity five proteins enrolled faecalis, with being highest, suggesting possible mechanisms ligands. exhibited higher than GINK, as values MICs lower those GINK. Thus, we performed assays However, they did not exhibit any significant variations (p > 0.05) membrane depolarization evaluated SEM, distortion deformation treated cells. Regarding its impact biofilm formation, it inhibited isolates, determined qRT-PCR. experiment revealed resulted reduction inflammation liver spleen increase survival rate. also improved liver-function tests decreased tumor necrosis factor-alpha immunostaining markers, interleukins (IL-1β IL-6), ELISA. can conclude could be promising compound should future preclinical clinical studies.

Language: Английский

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DPP-4 inhibitors and type 2 diabetes mellitus in Parkinson’s disease: a mutual relationship

Pharmacological Reports, Journal Year: 2023, Volume and Issue: 75(4), P. 923 - 936

Published: June 3, 2023

Language: Английский

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Cholinergic dysfunction in COVID-19: frantic search and hoping for the best

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2022, Volume and Issue: 396(3), P. 453 - 468

Published: Dec. 3, 2022

Language: Английский

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Deciphering the impact and mechanism of Trikatu, a spices-based formulation on alcoholic liver disease employing network pharmacology analysis and in vivo validation

Frontiers in Nutrition, Journal Year: 2022, Volume and Issue: 9

Published: Nov. 16, 2022

Trikatu Churna ( TC ) comprising Zingiber officinale rhizome, Piper longum , and nigrum fruit, is effective in treating liver diseases has high nutraceutical values. However, the efficacy of alcoholic disease (ALD) its mechanism remain largely unknown. This study evaluated hepatoprotective effects different doses as well to identify bioactive components determine their action against ethanol-induced ALD. A compound-target network analysis model was established potential compounds pathways that might regulate effects. Further, in-vivo studies were performed validate (200 mg/kg 400 b.w.) treatment management The revealed both dosages demonstrate significant p &gt; 0.0001) by improving body weight, total bilirubin, serum glutamic oxaloacetic transaminase (SGOT), pyruvic (SGPT), alkaline phosphate (ALP), cholesterol, protein, globulin, albumin, morphology. High-performance thin-layer chromatography (HPTLC) fingerprinting showed presence piperine. Network pharmacology identifies role regulating various signaling processes including Advanced glycation end products-receptor for advanced products (AGE-RAGE), Hypoxia-inducible factors (HIF-1), Nuclear factor kappa-light-chain-enhancer activated B cells (NF-Kappa B), Phosphatidylinositol 3-kinase/protein kinase (PI3K/Akt) exert anti-inflammatory, antioxidant anti-apoptotic managing Based on bioinformatics analysis, some key targets found be Prostaglandin-Endoperoxide Synthase 2 (PTGS2) or Cyclooxygenase-2 (COX-2), Sirtuin 1 (SRT1), caspase-3. These may serve a novel therapeutic option preclinical validation ethnopharmacological ALD further paved way researchers perform next-level translational clinical studies. in-depth experimental these bioinformatics-based results will give clearer picture mechanisms.

Language: Английский

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Functional Foods: A Promising Strategy for Restoring Gut Microbiota Diversity Impacted by SARS-CoV-2 Variants

Nutrients, Journal Year: 2023, Volume and Issue: 15(11), P. 2631 - 2631

Published: June 5, 2023

Natural herbs and functional foods contain bioactive molecules capable of augmenting the immune system mediating anti-viral functions. Functional foods, such as prebiotics, probiotics, dietary fibers, have been shown to positive effects on gut microbiota diversity function. The use has linked enhanced immunity, regeneration, improved cognitive function, maintenance microbiota, significant improvement in overall health. plays a critical role maintaining health disruptions its balance various problems. SARS-CoV-2 infection affect diversity, emergence variants poses new challenges combat virus. recognizes infects human cells through ACE2 receptors prevalent lung epithelial cells. Humans are prone because their respiratory gastrointestinal tracts rich microbial high levels TMPRSS2. This review article explores potential mitigating impact strategy these effects.

Language: Английский

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Curcumin as a hepatoprotective agent against chemotherapy-induced liver injury

Life Sciences, Journal Year: 2023, Volume and Issue: 332, P. 122119 - 122119

Published: Sept. 21, 2023

Despite significant advances in cancer therapeutics, chemotherapy remains the cornerstone of treatment for many tumors. Importantly, however, chemotherapy-induced toxicity, including hepatotoxicity, can lead to interruption or discontinuation potentially effective therapy. In recent years, special attention has been paid search complementary therapies mitigate toxicity. Although there is currently a lack specific interventions prevent hepatotoxicity chemotherapy-treated patients, polyphenol compound curcumin emerged as potential strategy overcome this adverse effect. Here we review, firstly, molecular and physiological mechanisms major risk factors hepatotoxicity. We then present an overview how by targeting mechanisms. Hepatotoxicity well-described side effect cytotoxic drugs that limit their clinical application. Inflammation oxidative stress are most common involved Several studies have shown could and/or palliate liver injury, mainly due its anti-inflammatory, antioxidant, antifibrotic hypolipidemic properties. Further investigation using bioavailable formulations warranted demonstrate efficacy hepatoprotective agent patients.

Language: Английский

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