Role and Therapeutic Targeting Strategies of Neutrophil Extracellular Traps in Inflammation

International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 5265 - 5287

Published: Sept. 1, 2023

Abstract: Neutrophil extracellular traps (NETs) are large DNA reticular structures secreted by neutrophils and decorated with histones antimicrobial proteins. As a key mechanism for to resist microbial invasion, NETs play an important role in the killing of microorganisms (bacteria, fungi, viruses). Although mostly known mediating killing, increasing evidence suggests that excessive induced stimulation physical chemical components, microorganisms, pathological factors can exacerbate inflammation organ damage. This review summarizes induction focuses on strategies inhibiting NETosis mechanisms involved pathogen evasion NETs. Furthermore, herbal medicine inhibitors nanodelivery improve efficiency inhibition levels Graphical Keywords: neutrophil traps, inflammation, targeted inhibition, nanotherapy,

Language: Английский

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Transplanting network pharmacology technology into food science research: A comprehensive review on uncovering food‐sourced functional factors and their health benefits

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2024, Volume and Issue: 23(5)

Published: Sept. 1, 2024

Abstract Network pharmacology is an emerging interdisciplinary research method. The application of network to reveal the nutritional effects and mechanisms active ingredients in food great significance promoting development functional food, facilitating personalized nutrition, exploring health effects. This article systematically reviews field science using a literature review progress discussed, factors on basis are explored. Additionally, limitations challenges future directions prospects proposed. serves as important tool action benefits food. It helps conduct in‐depth biological activities individual ingredients, composite foods, compounds assessment potential components. Moreover, it can help control enhance their functionality through relevant information during production processing samples guarantee safety. further analyzed summarized. Combining machine learning, artificial intelligence, clinical experiments, vitro validation, achievement transformation factor driven by for research.

Language: Английский

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Postbiotics Prepared Using Lactobacillus reuteri Ameliorates Ethanol‐Induced Liver Injury by Regulating the FXR/SHP/SREBP‐1c Axis

Molecular Nutrition & Food Research, Journal Year: 2024, Volume and Issue: 68(14)

Published: June 27, 2024

Scope While probiotics‐based therapies have exhibited potential in alleviating alcohol‐associated liver disease (ALD), the specific role of postbiotics derived from Lactobacillus reuteri ( L. ) ALD remains elusive. This study aims to investigate impact on ameliorating alcohol‐induced hepatic steatosis and underlying mechanisms. Methods results Using network pharmacology, elucidates targets pathways impacted by , identifying farnesoid X receptor (FXR) as a promising target for against ALD, lipid metabolism alcoholism act crucial associated with postbiotics‐targeting ALD. Furthermore, conducts histological biochemical analyses coupled LC/MS evaluate protective effects mechanisms Postbiotics may modulate bile acid vivo regulating FXR signaling, activating FXR/FGF15 pathway, influencing enterohepatic circulation acids (BAs). Subsequently, regulate activated BAs expression FXR‐mediated protein, including short regulatory partner (SHP) sterol element binding protein‐1c (SREBP‐1c), thereby mice Conclusion effectively alleviate ethanol‐induced FXR/SHP/SREBP‐1c axis, rigorously validated both vitro.

Language: Английский

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Opuntia ficus-indica (L.) Mill. - anticancer properties and phytochemicals: current trends and future perspectives

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 4, 2023

Cancer is a leading cause of mortality worldwide, and conventional cancer therapies such as chemotherapy radiotherapy often result in undesirable adverse effects. Natural products have emerged promising alternative for treatment, with comparatively fewer side effects reported. Opuntia ficus-indica (L.) Mill., member the Cactaceae family, contains diverse array phytochemicals, including flavonoids, polyphenols, betalains, tannins, which been shown to exhibit potent anticancer properties. Various parts plant, fruits, stems/cladodes, roots, demonstrated cytotoxic against malignant cell lines numerous studies. This review comprehensively summarizes attributes phytochemicals found highlighting their potential natural prevention treatment agents. Bibliometric metric analysis PubMed Scopus-retrieved data using VOSviewer well QDA provide further insights niche be explored. Most studies on its purified metabolites are related colorectal/colon cancer, followed by melanoma breast cancer. Very little attention has paid leukemia, thyroid, endometrial, liver, prostate it could considered an opportunity researchers explore O. these cancers. The most notable mechanisms expressed validated those apoptosis, cycle arrest (G0/G1 G2/M), Bcl-2 modulation, antiproliferative, oxidative stress-mediated mechanisms, cytochrome c. We also observed that cladodes fruits more studied than other plant parts, again opens explore. Further, line-based dominated, very few were animal-based experiments. Zebrafish model another platform However, seems like in-depth required ascertain clinical utility this biosustainable resource .

Language: Английский

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Trapped in the NETs: Multiple Roles of Platelets in the Vascular Complications Associated with Neutrophil Extracellular Traps

Cells, Journal Year: 2025, Volume and Issue: 14(5), P. 335 - 335

Published: Feb. 25, 2025

Neutrophil extracellular traps (NETs) have received significant attention in recent years for their role both the immune response and vascular damage associated with inflammation. Platelets been described as critical components of NETs since initial description this physio-pathological neutrophils. shown to play a dual responders also stimulators NETs. The direct interaction DNA leads entrapment platelets into NETs, phenomenon that significantly contributes thrombotic complications inflammation neutrophil activation, while paracrine stimulation neutrophils by has initiate process NET formation. In review, we provide comprehensive our current understanding molecular mechanisms underlying entrapping and, parallel, platelet-driven cellular responses promoting We then illustrate established examples contribution pathologies, describe important questions remain be answered regarding formation NET-dependent cardiovascular complication, highlight fundamental steps taken towards application platelets' development novel therapies.

Language: Английский

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Piperine–Chlorogenic Acid Hybrid Inhibits the Proliferation of the SK-MEL-147 Melanoma Cells by Modulating Mitotic Kinases

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(2), P. 145 - 145

Published: Jan. 19, 2023

Melanoma is considered the most aggressive form of skin cancer, showing high metastatic potential and persistent mortality rates despite introduction immunotherapy targeted therapies. Thus, it important to identify new drug candidates for melanoma. The design hybrid molecules, with different pharmacophore fragments combined in same scaffold, an interesting strategy obtaining multi-target more effective anticancer drugs. We designed nine compounds bearing piperine chlorogenic acid pharmacophoric groups evaluated their antitumoral on melanoma cells distinct mutational profiles SK-MEL-147, CHL-1 WM1366. identified compound named PQM-277 (3a) be cytotoxic one, inhibiting mitosis progression promoting accumulation pro-metaphase metaphase by altering expression genes that govern G2/M transition onset. Compound 3a downregulated FOXM1, CCNB1, CDK1, AURKA, AURKB, PLK1, upregulated CDKN1A. Molecular docking showed could interact CUL1-RBX1 complex, which activity necessary trigger molecular events essential FOXM1 transactivation and, turn, gene expression. In addition, effectively induced apoptosis increasing BAX/BCL2 ratio. Our findings demonstrate antitumor candidate prototype support further investigations evaluate its treatment, especially refractory cases BRAF/MEK inhibitors.

Language: Английский

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Insights into the potential benefits of triphala polyphenols toward the promotion of resilience against stress-induced depression and cognitive impairment

Current Research in Food Science, Journal Year: 2023, Volume and Issue: 6, P. 100527 - 100527

Published: Jan. 1, 2023

In response to environmental challenges, stress is a common reaction, but dysregulation of the can lead neuropsychiatric disorders, including depression and cognitive impairment. Particularly, there ample evidence that overexposure mental have lasting detrimental consequences for psychological health, function, ultimately well-being. fact, some individuals are resilient same stressor. A major benefit enhancing resilience in at-risk groups it may help prevent onset stress-induced health problems. potential therapeutic strategy maintaining healthy life address problems with botanicals or dietary supplements such as polyphenols. Triphala, also known Zhe Busong decoction Tibetan, well-recognized Ayurvedic polyherbal medicine comprising dried fruits from three different plant species. As promising food-sourced phytotherapy, triphala polyphenols been used throughout history treat variety medical conditions, brain maintenance. Nevertheless, comprehensive review still lacking. Here, primary objective this article provide an overview classification, safety, pharmacokinetics polyphenols, well recommendations development novel promoting susceptible individuals. Additionally, we summarize recent advances demonstrating beneficial by regulating 5-hydroxytryptamine (5-HT) brain-derived neurotrophic factor (BDNF) receptors, gut microbiota, antioxidant-related signaling pathways. Overall, scientific exploration warranted understand their efficacy. addition providing insights into mechanisms resilience, blood barrier (BBB) permeability systemic bioavailability need be improved research community. Moreover, well-designed clinical trials needed increase validity polyphenols' effects preventing treating impairment dysfunction.

Language: Английский

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Chlorogenic Acid from Burdock Roots Ameliorates Oleic Acid-Induced Steatosis in HepG2 Cells through AMPK/ACC/CPT-1 Pathway

Molecules, Journal Year: 2023, Volume and Issue: 28(21), P. 7257 - 7257

Published: Oct. 25, 2023

Hepatic steatosis can cause liver dysfunction and cell injury, on which natural functional factors are expected to be an effective approach for long-term intervention. However, the cellular molecular mechanisms unclear. Chlorogenic acid is a phenolic compound, regulate lipid metabolism abundant in burdock root. The aim of this study was investigate potential mechanism effect chlorogenic from root (ACQA) HepG2 cells. In study, we found that ACQA reduced number droplets levels oleic acid-treated Molecular mechanistic results showed enhanced CPT-1 expression by activating AMPK-related signaling pathways, concentrations Ca2+ cAMP were increased with intervention ACQA. addition, β-oxidation fatty acids, alanine transaminase aspartate transaminase, inhibited apoptosis Our studies elucidate novel enhances acids through AMPK/ACC/CPT-1 pathway protect against cells, provides insight into its as well strategies diseases.

Language: Английский

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The Cardioprotective Effects and Mechanisms of Astragalus-Safflower Herb Pairs on Coronary Heart Disease Identified by Network Pharmacology and Experimental Verification

Frontiers in Bioscience-Landmark, Journal Year: 2023, Volume and Issue: 28(5), P. 94 - 94

Published: May 22, 2023

Huang Qi (HQ, Astragalus) and Hong Hua (HH, Safflower), two Chinese herbal remedies, are widely used to treat coronary heart disease (CHD). However, the underlying mechanisms of this herb pair remain unclear. The aim study was determine potential synergistic effects Astragalus-Safflower in treatment CHD.Network pharamcology performed identify core components, targets, key genes (ASHP) for CHD. Enrichment analysis overlapping genes. Ultrahigh-performance liquid chromatography coupled with Q-Exactive MS/MS (UHPLC-QE-MS) detect blood component rat ASHP drug-containing serum, which is also considered be components ASHP. Molecular docking compounds proteins pyroptosis pathway mediated by NLR family pyrin domain containing 3 (NLRP3) inflammasomes. In vivo experiments were conducted verify effect mechanism CHD mice model.54 active 404 target identified from ASHP, 1576 targets 90 both. IL6, AKT1, IL1B, TP53, VEGFA, PTGS2, MMP9, CCL2, CXCL8 EGF hub Kyoto Encyclopedia Gene Genome (KEGG) revealed that NLRP3 inflammasome-mediated signaling one more critical pathways. UHPLC-QE-MS serum enrollment compound as calycosin isorhamnetin. showed quercetin, kaempferol, apigenin, isorhamnetin possessed good binding sites Caspase-1. Animal expression NLRP3, Caspase-1, GSDMD, IL-1β mRNA protein levels elevated mouse models CHD, decreased after intervention ASHP.ASHP can effectively may related inhibition pathway.

Language: Английский

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Exploration of the pharmacological mechanism of herbal medicines against hepatic disorder: A systematic review and network pharmacological analysis

Pharmacological Research - Natural Products, Journal Year: 2024, Volume and Issue: 3, P. 100048 - 100048

Published: April 30, 2024

Language: Английский

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