Quorum Sensing Orchestrates Antibiotic Drug Resistance, Biofilm Formation, and Motility in Escherichia coli and Quorum Quenching Activities of Plant-derived Natural Products: A Review DOI Creative Commons
Kevin Smith Puno Cabuhat, Llewelyn Sotto Moron-Espiritu

Journal of Pure and Applied Microbiology, Journal Year: 2022, Volume and Issue: 16(3), P. 1538 - 1549

Published: Aug. 19, 2022

Quorum sensing (QS) is a type of cell-to-cell communication that influenced by an increase in signaling molecules known as autoinducers, which correlated to the density microbial communities. In this review, we aim discuss and provide updates on different used Escherichia coli, such acyl-homoserine lactone (AHL), autoinducer-2 (AI-2), indole influence key phenotypes antibiotic drug resistance, biofilm formation, motility during quorum sensing. Based literature, E. coli have functions AHL was found cause modulation resistance inhibition formation motility. Meanwhile, AI-2 modulate On other hand, existing various plants possess phytochemicals can be alter QS its downstream swimming swarming motility, genes related curli production. However, exact physiological molecular mechanisms these natural compounds are still understudied. Understanding those therefore highly recommended conduct necessary step for future scholars develop drugs target actions QS-signaling receptors linked without putting bacteria under stress, thereby preventing development resistance.

Language: Английский

The Natural Product Curcumin as an Antibacterial Agent: Current Achievements and Problems DOI Creative Commons
Chongshan Dai, Jiahao Lin, Hui Li

et al.

Antioxidants, Journal Year: 2022, Volume and Issue: 11(3), P. 459 - 459

Published: Feb. 25, 2022

The rapid spread of antibiotic resistance and lack effective drugs for treating infections caused by multi-drug resistant bacteria in animal human medicine have forced us to find new antibacterial strategies. Natural products served as powerful therapeutics against bacterial infection are still an important source the discovery novel drugs. Curcumin, constituent turmeric, is considered safe oral consumption treat infections. Many studies showed that curcumin exhibited activities Gram-negative Gram-positive bacteria. action involves disruption membrane, inhibition production virulence factors biofilm formation, induction oxidative stress. These characteristics also contribute explain how acts a broad-spectrum adjuvant, which was evidenced markedly additive or synergistical effects with various types conventional antibiotics non-antibiotic compounds. In this review, we summarize properties, underlying molecular mechanism curcumin, discuss its combination use, nano-formulations, safety, current challenges towards development agent. We hope review provides valuable insight, stimulates broader discussions, spurs further developments around promising natural product.

Language: Английский

Citations

161
The interplay between diet and the gut microbiome: implications for health and disease DOI
Fiona C. Ross, Dhrati Patangia, Ghjuvan Micaelu Grimaud

et al.

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: 22(11), P. 671 - 686

Published: July 15, 2024

Language: Английский

Citations

116
Exploring the antivirulence potential of phenolic compounds to inhibit quorum sensing in Pseudomonas aeruginosa DOI
Emília Maria França Lima, Felipe Alves de Almeida, Uelinton Manoel Pinto

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(2)

Published: Jan. 11, 2025

Language: Английский

Citations

2
Quorum sensing interference by phenolic compounds – A matter of bacterial misunderstanding DOI Creative Commons
Emília Maria França Lima, Stephen C. Winans, Uelinton Manoel Pinto

et al.

Heliyon, Journal Year: 2023, Volume and Issue: 9(7), P. e17657 - e17657

Published: June 26, 2023

Over the past decade, numerous publications have emerged in literature focusing on inhibition of quorum sensing (QS) by plant extracts and phenolic compounds. However, there is still a scarcity studies that delve into specific mechanisms which these compounds inhibit QS. Thus, our question whether can QS or indirect manner to elucidate underlying involved. This study focused most studied system, namely, auto-inducer (AI-1) type QS, represented N-acyl-homoserine lactone (AHL) signals AHL-mediated responses. Here, we analyzed recent order understand how act at cellular level, sub-inhibitory concentrations, evaluated they may act. The biotechnological application inhibitors holds promising prospects for pharmaceutical food industries, serving as adjunct therapies prevention biofilms various surfaces.

Language: Английский

Citations

29
In Vitro Antibiofilm Activity of Resveratrol against Aeromonas hydrophila DOI Creative Commons
Ting Qin, Kai Chen, Bingwen Xi

et al.

Antibiotics, Journal Year: 2023, Volume and Issue: 12(4), P. 686 - 686

Published: March 31, 2023

Aeromonas hydrophila is a Gram-negative bacterium that widely exists in various aquatic environments and causes septicemia fish humans. Resveratrol, natural polyterpenoid product, has potential chemo-preventive antibacterial properties. In this study, we investigated the effect of resveratrol on A. biofilm formation motility. The results demonstrated resveratrol, at sub-MIC levels, can significantly inhibit hydrophila, was decreased with increasing concentrations. motility assay showed could diminish swimming swarming hydrophila. Transcriptome analyses (RNA-seq) treated 50 100 μg/mL respectively, presented 230 308 differentially expressed genes (DEGs), including 90 or 130 upregulated 178 downregulated genes. Among them, related to flagellar, type IV pilus chemotaxis were repressed. addition, mRNA virulence factors OmpA, extracellular proteases, lipases T6SS dramatically suppressed. Further analysis revealed major DEGs involved flagellar assembly bacterial pathways be regulated by cyclic-di-guanosine monophosphate (c-di-GMP)- LysR-Type transcriptional regulator (LTTR)-dependent quorum sensing (QS) systems. Overall, our indicate disturbing QS systems, used as promising candidate drug against motile Aeromonad septicemia.

Language: Английский

Citations

24
Deciphering the dynamics of methicillin-resistant Staphylococcus aureus biofilm formation: from molecular signaling to nanotherapeutic advances DOI Creative Commons

Nirmeen Aboelnaga,

Salma W. Elsayed,

Nehal Adel Abdelsalam

et al.

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: March 22, 2024

Abstract Methicillin-resistant Staphylococcus aureus (MRSA) represents a global threat, necessitating the development of effective solutions to combat this emerging superbug. In response selective pressures within healthcare, community, and livestock settings, MRSA has evolved increased biofilm formation as multifaceted virulence defensive mechanism, enabling bacterium thrive in harsh conditions. This review discusses molecular mechanisms contributing across its developmental stages, hence representing step forward developing promising strategies for impeding or eradicating biofilms. During staphylococcal development, cell wall-anchored proteins attach bacterial cells biotic abiotic surfaces; extracellular polymeric substances build scaffolds formation; cidABC operon controls lysis biofilm, proteases facilitate dispersal. Beside three main sequential stages (attachment, maturation, dispersal), unveils two unique process MRSA; multiplication exodus. We also highlighted quorum sensing cell-to-cell communication process, allowing distant adapt conditions surrounding biofilm. S. , is mediated by autoinducing peptides (AIPs) signaling molecules, with accessory gene regulator system playing pivotal role orchestrating production AIPs various factors. Several inhibitors showed anti-virulence antibiofilm effects that vary type function according targeted molecule. Disrupting architecture sessile are crucial steps prevent colonization on other surfaces organs. context, nanoparticles emerge efficient carriers delivering antimicrobial agents throughout architecture. Although metal-based have been previously used combatting biofilms, non-degradability toxicity human body presents real challenge. Therefore, organic conjunction proposed strategy against As nanotherapeutics continue gain recognition an strategy, more could offer solution biofilm-mediated resistance.

Language: Английский

Citations

16
Comparative assessment of phenolic composition profile and biological activities of green extract and conventional extracts of Salvia sclarea DOI Creative Commons
Mohammed Mansour Quradha, Mehmet Emin Duru, Selçuk Küçükaydın

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 22, 2024

Abstract In recent years, there have been an attempt to develop safe and environmental friendly solvents replace conventional solvents, use for extraction bioactive compounds from natural sources. A current investigation involved the preparation of green, methanolic, ultrasonic extracts S. sclarea , compared their phenolic profiling using HPLC–DAD, antibacterial, antioxidant, enzyme inhibition activities. The HPLC–DAD analysis revealed that Rosmarinic acid was main content in all extracts, with Ellagic only present green extract. extract exhibited superior anti-biofilm activity against Aureus E. Faecalis other at MIC concentration. Furthermore, also displayed highest swarming motility P. Aeruginosa range 68.0 ± 2.1 (MIC) 19.5 0.6 (MIC/4). better inhibitory BChE (with IC 50 = 131.6 0.98 µg/mL) AChE 47.00 1.50%) extracts; while, showed strong violacein production by C. Violaceum a 05.5 0.1 (MIC/32) 100 0.00 (MIC), followed 15.0 0.5 (MIC/8) additionally, methanoic significant urease (IC 171.6 0.95 µg/mL 5 0 187.5 1.32 respectively. Both methanolic considerable antioxidant activities, as β -carotene-linoleic 5.61 0.47 5.37 0.27 µg/mL), DPPH · 19.20 0.70 16.31 0.23 ABTS + 8.64 0.63 6.50 0.45 CUPRAC (A 17.22 0.36 12.28 0.12 respectively, likewise performing metal chelating extracts. is reported cost effective solvent free method extracting products produces toxic chemicals. This could be used industries renewable method.

Language: Английский

Citations

10
Capsaicin from chili peppers and its analogues and their valued applications: An updated literature review DOI
Na Wang, Xiang Zhou,

Taihong Zhang

et al.

Food Research International, Journal Year: 2025, Volume and Issue: 208, P. 116034 - 116034

Published: Feb. 25, 2025

Language: Английский

Citations

1
New insights into antimicrobial and antibiofilm effects of edible mushrooms DOI
Ashaimaa Y. Moussa, Shaimaa Fayez, Hang Xiao

et al.

Food Research International, Journal Year: 2022, Volume and Issue: 162, P. 111982 - 111982

Published: Sept. 30, 2022

Language: Английский

Citations

33
Anti-Quorum Sensing and Anti-Biofilm Activity of Pelargonium × hortorum Root Extract against Pseudomonas aeruginosa: Combinatorial Effect of Catechin and Gallic Acid DOI Creative Commons
Fatma M. Abdel Bar, Manal A. Alossaimi, Engy Elekhnawy

et al.

Molecules, Journal Year: 2022, Volume and Issue: 27(22), P. 7841 - 7841

Published: Nov. 14, 2022

HPLC-UV was used to compare the major constituents of two Pelargonium × hortorum cultivars and sidoides root extract. It revealed presence catechin gallic acid in high concentrations absence umckalin P. extracts. The antibacterial activity these extracts screened against 19 Pseudomonas aeruginosa clinical isolates. showed lowest MIC values (512-1024 µg/mL). This concluded be attributable acid. anti-biofilm catechin, acid, their combination examined by a crystal violet assay. reduced percentage strong moderate biofilm-forming isolates from 52.63% 5.26%. impact on lasI lasR genes expression using qRT-PCR simultaneous docking LasR protein explored. downregulated gene eight six isolates, respectively, greatest score. Additionally, vivo protection capability this infected mice enhancement survival rate. Our study potential biofilm quorum-sensing-inhibitory as novel alternative inhibit bacterial pathogenicity.

Language: Английский

Citations

31