The Spread of SARS-CoV-2 Omicron Variant in CALABRIA: A Spatio-Temporal Report of Viral Genome Evolution DOI Creative Commons

Claudia Veneziano,

Nadia Marascio, Carmela De Marco

et al.

Viruses, Journal Year: 2023, Volume and Issue: 15(2), P. 408 - 408

Published: Jan. 31, 2023

We investigated the evolution of SARS-CoV-2 spread in Calabria, Southern Italy, 2022. A total 272 RNA isolates from nasopharyngeal swabs individuals infected with were sequenced by whole genome sequencing (N = 172) and/or Sanger 100). Analysis diffusion Omicron variants Calabria revealed prevalence 10 different sub-lineages (recombinant BA.1/BA.2, BA.1, BA.1.1, BA.2, BA.2.9, BA.2.10, BA.2.12.1, BA.4, BA.5, BE.1). observed that presented a similar trend as some notable exceptions: BA.1 disappeared April but not rest Italy; recombinant BA.1/BA.2 showed higher frequency (13%) than Italy (0.02%); BA.4 and BA.5 emerged later other Italian regions. In addition, 16 non-canonical mutations S protein 151 non-structural proteins. Most occurred mainly whereas or accessory proteins (ORF1ab, ORF3a, ORF8 N) identified BA.2 sub-lineages. conclusion, data reported here underscore importance monitoring entire genome.

Language: Английский

An update on lateral flow immunoassay for the rapid detection of SARS-CoV-2 antibodies DOI Creative Commons
Lucia Spicuzza, Davide Campagna, Chiara Di Maria

et al.

AIMS Microbiology, Journal Year: 2023, Volume and Issue: 9(2), P. 375 - 401

Published: Jan. 1, 2023

Over the last three years, after outbreak of COVID-19 pandemic, an unprecedented number novel diagnostic tests have been developed. Assays to evaluate immune response SARS-CoV-2 widely considered as part control strategy. The lateral flow immunoassay (LFIA), detect both IgM and IgG against SARS-CoV-2, has studied a point-of-care (POC) test. Compared laboratory tests, LFIAs are faster, cheaper user-friendly, thus available also in areas with low economic resources. Soon onset numerous kits for rapid antibody detection were put on market emergency use authorization. However, since then, scientists tried better define accuracy these their usefulness different contexts. In fact, while during first phase pandemic auxiliary molecular diagnosis COVID-19, successively became tool seroprevalence surveillance address infection policies. When 2021 massive vaccination campaign was implemented worldwide, interest LFIA reemerged due need establish extent longevity immunization vaccinated population priorities guide health policies low-income countries limited access vaccines. Here, we summarize accuracy, advantages limits POC detection, highlighting efforts that made improve this technology over few years.

Language: Английский

Citations

14
An overview of electrochemical biosensors used for COVID-19 detection DOI
Tatiana Lima Valério, Raquel Anastácio, Stella Schuster da Silva

et al.

Analytical Methods, Journal Year: 2024, Volume and Issue: 16(15), P. 2164 - 2176

Published: Jan. 1, 2024

This short review presents the latest advances in field of electrochemical biosensors, focusing particularly on impedimetric biosensors for direct measurement analytes.

Language: Английский

Citations

5
From Detection to Protection: Antibodies and Their Crucial Role in Diagnosing and Combatting SARS-CoV-2 DOI Creative Commons
Anoop Kumar, Prajna Tripathi, Prashant Kumar

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(5), P. 459 - 459

Published: April 25, 2024

Understanding the antibody response to SARS-CoV-2, virus responsible for COVID-19, is crucial comprehending disease progression and significance of vaccine therapeutic development. The emergence highly contagious variants poses a significant challenge humoral immunity, underscoring necessity grasping intricacies specific antibodies. This review emphasizes pivotal role antibodies in shaping immune responses their implications diagnosing, preventing, treating SARS-CoV-2 infection. It delves into kinetics characteristics explores current antibody-based diagnostics, discussing strengths, clinical utility, limitations. Furthermore, we underscore potential SARS-CoV-2-specific antibodies, various therapies such as monoclonal polyclonal anti-cytokines, convalescent plasma, hyperimmunoglobulin-based therapies. Moreover, offer insights vaccines, emphasizing neutralizing order confer immunity along with emerging concern (VOCs) circulating Omicron subvariants. We also highlight challenges field, risks antibody-dependent enhancement (ADE) shed light on associated original antigenic sin (OAS) effect long COVID. Overall, this intends provide valuable insights, which are advancing sensitive diagnostic tools, identifying efficient therapeutics, developing effective vaccines combat evolving threat global scale.

Language: Английский

Citations

5
A Quantitative Detection Algorithm for Multi-Test Line Lateral Flow Immunoassay Applied in Smartphones DOI Creative Commons
Shenglan Zhang,

Xincheng Jiang,

Siqi Lu

et al.

Sensors, Journal Year: 2023, Volume and Issue: 23(14), P. 6401 - 6401

Published: July 14, 2023

The traditional lateral flow immunoassay (LFIA) detection method suffers from issues such as unstable results and low quantitative accuracy. In this study, we propose a novel multi-test line using smartphone-based SAA strips. Following the utilization of image processing techniques to extract analyze pigments on strips, analysis was conducted. Experimental setups with controlled lighting conditions in dark box were designed capture samples smartphones different specifications for analysis. algorithm's sensitivity robustness validated by introducing noise samples, performance strips algorithms determined. experimental demonstrate that proposed based achieves an accuracy rate 94.23% 260 which is comparable methods but higher stability lower algorithm complexity.

Language: Английский

Citations

12
Advancing Microfluidic Immunity Testing Systems: New Trends for Microbial Pathogen Detection DOI Creative Commons
Yiran Wang, Jingwei Chen, Yule Zhang

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(14), P. 3322 - 3322

Published: July 15, 2024

Pathogenic microorganisms play a crucial role in the global disease burden due to their ability cause various diseases and spread through multiple transmission routes. Immunity tests identify antigens related these pathogens, thereby confirming past infections monitoring host's immune response. Traditional pathogen detection methods, including enzyme-linked immunosorbent assays (ELISAs) chemiluminescent immunoassays (CLIAs), are often labor-intensive, slow, reliant on sophisticated equipment skilled personnel, which can be limiting resource-poor settings. In contrast, development of microfluidic technologies presents promising alternative, offering automation, miniaturization, cost efficiency. These advanced methods poised replace traditional by streamlining processes enabling rapid, high-throughput immunity testing for pathogens. This review highlights latest advancements systems designed rapid testing, incorporating immunosensors, single molecule arrays (Simoas), lateral flow assay (LFA), smartphone integration. It focuses key pathogenic such as SARS-CoV-2, influenza, ZIKA virus (ZIKV). Additionally, discusses challenges, commercialization prospects, future directions advance infectious detection.

Language: Английский

Citations

4
Siphon-based multichannel acoustofluidic separator for rapid and multiplexed biomolecule detection DOI Creative Commons
Cooper P. Thome,

Creighton T. Tisdale,

C. Wyatt Shields

et al.

Device, Journal Year: 2025, Volume and Issue: unknown, P. 100727 - 100727

Published: March 1, 2025

Language: Английский

Citations

0
Engineering and Deployment of Sars-Cov-2 Recombinant Protein-Based Covid-19 Detection Kits in Ecuador DOI
Andrea Aluisa, Marcela Orozco,

Stefany Olmedo Karolys

et al.

Lecture notes in networks and systems, Journal Year: 2025, Volume and Issue: unknown, P. 299 - 315

Published: Jan. 1, 2025

Language: Английский

Citations

0
Microarray-Based Detection of Antibodies against SARS-CoV-2 Proteins, Common Respiratory Viruses and Type I Interferons DOI Creative Commons
Elena Savvateeva, М. А. Филиппова, Vladimir T. Valuev-Elliston

et al.

Viruses, Journal Year: 2021, Volume and Issue: 13(12), P. 2553 - 2553

Published: Dec. 20, 2021

A microarray-based assay to detect IgG and IgM antibodies against betacoronaviruses (SARS-CoV-2, SARS, MERS, OC43, HKU1), other respiratory viruses type I interferons (IFN-Is) was developed. This multiplex applied track antibody cross-reactivity due previous contact with similar identify IFN-Is as the markers for severe COVID-19. In total, 278 serum samples from convalescent plasma donors, COVID-19 patients in intensive care unit (ICU) who recovered mild/moderate COVID-19, vaccine recipients, prepandemic pandemic autoimmune endocrine disorders, a heterogeneous cohort including healthy individuals chronically ill were analyzed. The anti-SARS-CoV-2 microarray results agreed well ELISA results. Regarding ICU patients, autoantibodies detected 10.5% of samples, found simultaneously contain more than two different viruses. Cross-reactivity between SARS-CoV-2 nucleocapsid OC43 HKU1 spike proteins observed, resulting positive signals disorders. presence absence RBD should be interpreted caution.

Language: Английский

Citations

26
Creation of Standardized Common Data Elements for Diagnostic Tests in Infectious Disease Studies: Semantic and Syntactic Mapping DOI Creative Commons
Caroline Stellmach, Sina M. Hopff, Thomas Jaenisch

et al.

Journal of Medical Internet Research, Journal Year: 2024, Volume and Issue: 26, P. e50049 - e50049

Published: June 10, 2024

Background It is necessary to harmonize and standardize data variables used in case report forms (CRFs) of clinical studies facilitate the merging sharing collected patient across several studies. This particularly true for that focus on infectious diseases. Public health may be highly dependent findings such Hence, there an elevated urgency generate meaningful, reliable insights, ideally based a high sample number quality data. The implementation core elements incorporation interoperability standards can creation harmonized sets. Objective study’s objective was compare, harmonize, focused diagnostic tests as part CRFs 6 international diseases order to, ultimately, then make available panstudy common (CDEs) ongoing future foster comparability trials. Methods We reviewed compared metadata comprised collection all disease under consideration identify CDEs. examined availability semantic standard codes within Systemized Nomenclature Medicine - Clinical Terms, National Cancer Institute Thesaurus, Logical Observation Identifiers Names Codes system unambiguous representation testing information makes up proposed 2 models incorporate syntactic identified Results Of 216 were considered scope analysis, we 11 CDEs describe (in particular, serology sequencing) diseases: viral lineage/clade; test date, type, performer, manufacturer; target gene; quantitative qualitative results; specimen identifier, date. Conclusions identification first step facilitating exchange possible subset (and with that, large research projects) shared analysis increase power findings. path harmonization standardization study interest paved ways. First, map terminologies ensures each element’s (variable’s) definition it has single, unique interpretation Second, these assisted by “wrapping” them format, Fast Health care Interoperability Resources or Data Interchange Standards Consortium’s Acquisition Harmonization Model.

Language: Английский

Citations

3
SARS-CoV-2 seropositivity amongst healthcare workers in South Africa during the Omicron wave: natural infection versus vaccination DOI
Daniel Hoffmann, Pieter Meyer, Catherine Worsley

et al.

Journal of Clinical Pathology, Journal Year: 2025, Volume and Issue: unknown, P. jcp - 209722

Published: Jan. 16, 2025

Concerns over population-level immunity have been heightened with each successive wave of COVID-19, prompting questions about whether it is primarily derived from vaccination efforts or previous natural infections the virus. We wished to determine seroprevalence SARS-CoV-2 antibodies among healthcare workers (HCWs) in Pretoria (Tshwane), South Africa, and establish they were infection. Serum samples collected HCWs during fourth COVID-19 between 1 December 2021 13 March 2022. The tested using Abbott Spike IgG (S-IgG), IgM (S-IgM) Nucleocapsid (NC-IgG) kits. Of 221 participants, 76% (n=168) women 24% (n=53) men. A total 96.4% (n=213) participants vaccinated. Natural infection-derived detected 23% (n=51) vaccine-derived 74% (n=164) HCWs. Even after three waves most their detectable vaccination. Vaccination remains an essential tool protect patients

Language: Английский

Citations

0