Ageing Research Reviews, Journal Year: 2023, Volume and Issue: 90, P. 102026 - 102026
Published: July 31, 2023
Since the discovery of mechanosensitive Piezo1 channel in 2010, there has been a significant amount research conducted to explore its regulatory role physiology and pathology various organ systems. Recently, growing body compelling evidence emerged linking activity health disease central nervous system. However, exact mechanisms underlying these associations remain inadequately comprehended. This review systematically summarizes current on implications for system mechanobiology, retrospects results demonstrating cell types within system, including neural stem cells, neurons, oligodendrocytes, microglia, astrocytes, brain endothelial cells. Furthermore, discusses understanding involvement disorders, such as Alzheimer's disease, multiple sclerosis, glaucoma, stroke, glioma.
Language: Английский
Citations
18Mechanobiology of Dental Pulp Cells
Cells, Journal Year: 2024, Volume and Issue: 13(5), P. 375 - 375
Published: Feb. 21, 2024
The dental pulp is the inner part of tooth responsible for properly functioning during its lifespan. Apart from very big biological heterogeneity cells, microenvironments differ a lot in context mechanical properties-ranging 5.5 kPa to around 100 GPa dentin and enamel. This physical complexity plays key role physiology turn, great target variety therapeutic approaches. First all, mechanisms are crucial pain propagation process surface nerves inside pulp. On other hand, modulation environment affects cells thus important regenerative medicine. In present review, we describe physiological significance biomechanical processes pathology Moreover, couple those phenomena with recent advances fields bioengineering pharmacology aiming control reduce pain, enhance differentiation into desired lineages. reviewed literature shows progress topic pulp-although mainly vitro. few positions, it leaves gap necessary filling studies providing vivo.
Language: Английский
Citations
6Mechanosensitive Piezo1 and Piezo2 ion channels in craniofacial development and dentistry: Recent advances and prospects
Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 13
Published: Oct. 21, 2022
Mechanical forces play important roles in many biological processes and there is increasing interest understanding of these roles. Mechanotransduction the process by which mechanical stimuli are converted to biochemical signals through specific mechanisms, this results activation downstream signaling pathways with effects on cell behaviors. This review systematically summarizes current mechanosensitive Piezo1 Piezo2 ion channels craniofacial bone, tooth, periodontal tissue, presenting latest relevant evidence implications for potential treatments managements dental orofacial diseases deformities. The widely expressed various cells tissues have essential functions mechanosensation mechanotransduction. These an active role physiological pathological processes, such as growth development, mechano-stimulated bone homeostasis mediation inflammatory responses. Emerging indicates expression tissue-derived stem suggests that they function sensation transduction, dentin mineralization remodeling modulate orthodontic tooth movement.
Language: Английский
Citations
20Dentin Mechanobiology: Bridging the Gap between Architecture and Function
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5642 - 5642
Published: May 22, 2024
It is remarkable how teeth maintain their healthy condition under exceptionally high levels of mechanical loading. This suggests the presence inherent adaptation mechanisms within structure to counter constant stress. Dentin, situated between enamel and pulp, plays a crucial role in mechanically supporting tooth function. Its intermediate stiffness viscoelastic properties, attributed its mineralized, nanofibrous extracellular matrix, provide flexibility, strength, rigidity, enabling it withstand loading without fracturing. Moreover, dentin’s unique architectural features, such as odontoblast processes dentinal tubules spatial compartmentalization odontoblasts dentin sensory neurons contribute distinctive perception external stimuli while acting defensive barrier for dentin-pulp complex. Since architecture governs functions nociception repair response stimuli, understanding mechanobiology developing treatments pain management dentin-associated diseases regeneration. review discusses physical features regulate mechano-sensing, focusing on mechano-sensitive ion channels. Additionally, we explore advanced vitro platforms that mimic providing deeper insights into fundamental mechanobiological phenomena laying groundwork effective mechano-therapeutic strategies diseases.
Language: Английский
Citations
4TRPV4 activation in Schwann cells mediates mechanically induced pain of oral cancer
Frontiers in Pain Research, Journal Year: 2025, Volume and Issue: 6
Published: March 12, 2025
Introduction Patients with oral cancer often experience intense functional pain due to mechanical stimulation at the site. The role of mechanosensitive ion channels in pain, such as TRPV4, is not fully understood. Objectives Our objective was investigate Schwann cell TRPV4 pain. Methods We examined impact inhibition on NU/J and C57BL/6J mice injected human tongue line (HSC-3) mouse (MOC2) hind paw or tongue. Mechanical heat sensitivity were assessed using von Frey Hargreaves tests, respectively. expression activity cells analyzed immunohistochemistry, qRT-PCR, Ca 2+ imaging, patch-clamp electrophysiology. effect activation responses evaluated a piezo stimulator. Conditioned media (CM) from TRPV4-activated into evaluate contribution hypersensitivity. Results reduced nociception dose-dependently without affecting sensitivity. also decreased facial mice. expressed mainly plasma membrane induced whole-cell currents cells. Mechanoactivated inhibited by antagonist HC-067047. CM hypersensitivity mice, which blocked pre-treatment Conclusion plays mediating mechanically cancer.
Language: Английский
Citations
0Beyond hydrodynamics: The role of ion channels in dentine hypersensitivity
Journal of Dentistry, Journal Year: 2025, Volume and Issue: unknown, P. 105745 - 105745
Published: April 1, 2025
Language: Английский
Citations
0Piezo1 in Digestive System Function and Dysfunction
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(16), P. 12953 - 12953
Published: Aug. 19, 2023
Piezo1, a non-selective cation channel directly activated by mechanical forces, is widely expressed in the digestive system and participates biological functions physiologically pathologically. In this review, we summarized latest insights on Piezo1’s cellular effect across entire system, discussed role of Piezo1 various aspects including ingestion digestion, material metabolism, enteric nervous intestinal barrier, inflammatory response within system. The goal comprehensive review to provide solid foundation for future research about
Language: Английский
Citations
10PIEZO1 Promotes Odontoblast-Mediated Reactionary Dentinogenesis via SEMA3A
Journal of Dental Research, Journal Year: 2024, Volume and Issue: 103(9), P. 889 - 898
Published: June 24, 2024
Located at the interface of dentin-pulp complex, odontoblasts are specialized cells responsible for dentin synthesis and nociceptive signal detection in response to external stimuli. Recent studies have shown that mechanosensitive ion channel PIEZO1 is involved bone formation remodeling through influx calcium ions, it abundantly expressed odontoblasts. However, specific role reactionary dentinogenesis underlying mechanisms remain elusive. In this study, we found intense expression plasma membrane cytoplasm healthy human third molars, mouse mandibular odontoblast-like (hOBLCs). hOBLCs, positively regulated DSPP, DMP1, COL1A1 Ca
Language: Английский
Citations
3Immunolocalization of the mechanogated ion channels PIEZO1 and PIEZO2 in human and mouse dental pulp and periodontal ligament
The Anatomical Record, Journal Year: 2023, Volume and Issue: 307(5), P. 1960 - 1968
Published: Nov. 17, 2023
Abstract PIEZO1 and PIEZO2 are essential components of mechanogated ion channels, which required for mechanotransduction biological processes associated with mechanical stimuli. There is evidence the presence in teeth periodontal ligaments, especially cell lines mice, but human studies almost nonexistent. Decalcified permanent mouse molars were processed immunohistochemical detection PIEZO2. Confocal laser microscopy was used to examine co‐localization PIEZO 1 vimentin (a marker differentiated odontoblasts) teeth. In outer layer dental pulp, abundant PIEZO1‐ PIEZO2‐positive cells found that had no odontoblast morphology vimentin‐negative. Based on their morphology, location, absence positivity, they identified as pulp stem or pre‐odontoblasts. However, ubiquitously detected colocalized odontoblasts. Intense immunoreactivity has been observed murine ligaments. Our findings suggest may be mechanosensors/mechanotransducers odontoblasts, well transmission forces by ligament humans mice.
Language: Английский
Citations
7Piezo Mediates the Mechanosensation and Injury-Repair of Pulpo-Dentinal Complex
International Dental Journal, Journal Year: 2023, Volume and Issue: 74(1), P. 71 - 80
Published: Oct. 11, 2023
The aim of this research was to investigate the functions Piezo channels in dentin defect, including mechanical signalling and odontoblast responses.
Language: Английский
Citations
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