Odor and reward-evoked GABAergic neuronal activity in the basal forebrain influences olfactory-guided behavior in mice DOI Creative Commons
Stephen J. Moss, Evelyne K. Tantry,

Elaine H. Le

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Sept. 4, 2022

ABSTRACT Sensory perception relies on the flexible detection and interpretation of stimuli across variable contexts, conditions, behavioral states. The basal forebrain is a hub for state regulation, supplying dense cholinergic GABAergic projections to various brain regions involved in sensory processing. Of neurons forebrain, parvalbumin (PV) somatostatin (SST) subtypes serve opposing roles towards regulating To elucidate role circuits sensory-guided behavior, we investigated signaling dynamics during odor-guided decision-making. We used fiber photometry record cell type-specific activity an odor discrimination task correlated temporal patterns PV SST neuronal with olfactory performance. found that while both PV-expressing SST-expressing were activated by odors, selectively suppressed reward whereas activated. Notably, chemogenetic inhibition BF modestly altered decision bias favor reward-seeking optogenetic presentations improved accuracy. Together, these results suggest bidirectional neuron distinctly influence decision-making guided behavior. SIGNIFICANCE STATEMENT This study reveals distinct Fiber shows parvalbumin-expressing are rewards, somatostatin-expressing activated, establishing unique recruitment reinforcement. Chemogenetic interventions demonstrate divergent behavior perception. research provides new insights into how shape

Language: Английский

Signaling mechanisms underlying activity‐dependent integration of adult‐born neurons in the mouse olfactory bulb DOI
Suyang Bao,

Juan M. Romero,

Benjamin D. W. Belfort

et al.

genesis, Journal Year: 2024, Volume and Issue: 62(2)

Published: March 30, 2024

Summary Adult neurogenesis has fascinated the field of neuroscience for decades given prospects harnessing mechanisms that facilitate rewiring and/or replacement adult brain tissue. The subgranular zone hippocampus and subventricular lateral ventricle are two main areas in exhibit ongoing neurogenesis. Of these, adult‐born neurons within olfactory bulb have proven to be a powerful model studying circuit plasticity, providing broad accessible avenue into neuron development, migration, continued integration This review focuses on some recognized molecular signaling underlying activity‐dependent development. Notably, activity behavioral states contribute plasticity through sensory centrifugal inputs, which calcium‐dependent transcriptional programs, local translation, neuropeptide play important roles. also highlights needed investigation better understand remarkable phenomenon integration.

Language: Английский

Citations

1
Optotagging and Characterization of GABAergic Rostral Ventromedial Medulla (RVM) neurons DOI Creative Commons
Taylor Follansbee,

Henry Chang,

Mirela Lodi Carstens

et al.

Molecular Pain, Journal Year: 2024, Volume and Issue: unknown

Published: July 25, 2024

The transmission of nociceptive and pruriceptive signals in the spinal cord is greatly influenced by descending modulation from brain areas such as rostral ventromedial medulla (RVM). Within RVM three classes neurons have been discovered which are relevant to pain modulation, On, Off, Neutral cells. These were due their functional response stimulation. On cells excited, Off inhibited, no noxious Since these identified characteristics it has difficult molecularly identify them. In present study, we leverage our ability perform optotagging within determine whether GABAergic. We found that 27.27% cells, 47.37% 42.6% results demonstrate represent a heterogeneous population provide reliable technique for molecular identification neurons.

Language: Английский

Citations

1
JUMP: replicability analysis of high-throughput experiments with applications to spatial transcriptomic studies DOI Creative Commons
Pengfei Lyu, Yan Li, Xiaoquan Wen

et al.

Bioinformatics, Journal Year: 2023, Volume and Issue: 39(6)

Published: June 1, 2023

Replicability is the cornerstone of scientific research. The current statistical method for high-dimensional replicability analysis either cannot control false discovery rate (FDR) or too conservative.

Language: Английский

Citations

2
Long-range GABAergic inhibition modulates spatiotemporal dynamics of the output neurons in the olfactory bulb DOI Open Access
Pablo S. Villar,

Ruilong Hu,

Ricardo C. Araneda

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2020, Volume and Issue: unknown

Published: July 10, 2020

SUMMARY Local interneurons of the olfactory bulb (OB) are densely innervated by long-range GABAergic neurons from basal forebrain (BF), suggesting that this top-down inhibition regulates early processing in system. However, how inputs modulate OB output neurons, mitral/tufted cells, is unknown. Here, acute brain slices, we show optogenetic activation BF produced distinct local circuit effects can influence activity cells spatiotemporal domains. Activation axons a fast disinhibition consistent with rapid and synchronous release GABA onto glomerular inframitral circuits OB, which also reduced spike precision response to simulated stimuli. In addition, modulated oscillations layer-specific manner. The intensity locally evoked θ was decreased upon circuit, while γ were granule cells. Furthermore, input dendrodendritic Together, these results suggest well suited temporal spatial aspects circuits.

Language: Английский

Citations

5
The Response Dynamics and Function of Cholinergic and GABAergic Neurons in the Basal Forebrain During Olfactory Learning DOI Creative Commons

Ping Zhou,

Penglai Liu,

Ying Zhang

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2022, Volume and Issue: 16

Published: July 27, 2022

Modulation of neural circuits is essential for flexible sensory perception and decision-making in a changing environment. Cholinergic GABAergic projections to the olfactory system from horizontal limb diagonal band Broca (HDB) basal forebrain are crucial odor detection learning. Although studies have demonstrated that HDB neurons respond during learning, how cholinergic differ their response dynamics roles learning remains unclear. In this study, we examined profiles these two subpopulations passive exposure associative We show excitatory responses both tended habituate repeated exposure. However, while habituated were also observed go-go task, there was no such habituation neurons. Moreover, S+ S- trials diverged once mice learned go/no-go task. Furthermore, chemogenetic inactivation impaired discrimination. Together, findings suggest reflect attention positive reinforcement may regulate discrimination via top-down inputs system.

Language: Английский

Citations

3
Loss of GABA co-transmission from cholinergic neurons impairs behaviors related to hippocampal, striatal, and medial prefrontal cortex functions DOI Creative Commons
R. Oliver Goral, Kathryn M. Harper, Briana J. Bernstein

et al.

Frontiers in Behavioral Neuroscience, Journal Year: 2022, Volume and Issue: 16

Published: Nov. 24, 2022

Introduction: Altered signaling or function of acetylcholine (ACh) has been reported in various neurological diseases, including Alzheimer's disease, Tourette syndrome, epilepsy among others. Many neurons that release ACh also co-transmit the neurotransmitter gamma-aminobutyrate (GABA) at synapses hippocampus, striatum, substantia nigra, and medial prefrontal cortex (mPFC). Although transmission is crucial for higher brain functions such as learning memory, role co-transmitted GABA from remains unknown. Thus, overarching goal this study was to investigate how a systemic loss co-transmission affected behavioral performance mice. Methods: To do this, we used conditional knock-out mouse vesicular transporter (vGAT) crossed with ChAT-Cre driver line selectively ablate synapses. In comprehensive series standardized assays, compared Cre-negative control mice Cre-positive vGAT both sexes. Results: Loss did not disrupt animal's sociability, motor skills sensation. However, absence co-transmission, found significant alterations social, spatial fear memory well reduced reliance on striatum-dependent response strategies T-maze. addition, male knockout (CKO) showed increased locomotion. Discussion: Taken together, leads deficits behaviors. Therefore, propose ACh/GABA modulates neural circuitry involved

Language: Английский

Citations

3
Basal forebrain modulation of olfactory coding in vivo DOI Creative Commons
Juan Pablo Venegas, Marcela Díaz,

Laura Orellana-Garcia

et al.

International journal of psychological research, Journal Year: 2023, Volume and Issue: 16(2), P. 62 - 86

Published: Aug. 23, 2023

Sensory perception is one of the most fundamental brain functions, allowing individuals to properly interact and adapt a constantly changing environment. This process requires integration bottom-up topdown neuronal activity, which centrally mediated by basal forebrain, region that has been linked series cognitive processes such as attention alertness. Here, we review latest research using optogenetic approaches in rodents vivo electrophysiological recordings are shedding light on role this region, regulating olfactory processing decisionmaking. Moreover, summarize evidence highlighting anatomical physiological differences forebrain with autism spectrum disorder, could underpin sensory abnormalities they exhibit, propose line potential opportunity understand neurobiological basis disorder.La percepción sensorial es una de las funciones cerebrales más fundamentales, permitiendo los individuos interactuar manera apropiada con el entorno y adaptarse un ambiente en constante cambio. Este proceso requiere la integración actividad ascendente descendente, que mediada por cerebro (BF), región cerebral ha sido asociada serie procesos cognitivos, como estados atención alerta.En este trabajo revisamos últimas investigaciones han utilizado optogenética registros electrofisiológicos iluminado rol del BF procesamiento olfatorio toma decisiones. Además, resumimos literatura destaca alteraciones fisiológicas anatómicas trastornos espectro autista, podrían subyacer anormalidades presentan, proponemos esta línea investigación posible oportunidad para entender bases neurobiológicas trastorno.

Citations

1
Olfactory integration and odor perception DOI
Patricia Duchamp‐Viret, Nicola Kuczewski, Christine Baly

et al.

Elsevier eBooks, Journal Year: 2022, Volume and Issue: unknown, P. 149 - 204

Published: Aug. 19, 2022

Language: Английский

Citations

2
Dynamic cholinergic tone in the basal forebrain reflects reward-seeking and reinforcement during olfactory behavior DOI Creative Commons

Elizabeth Hanson,

Katie L. Brandel-Ankrapp, Benjamin R. Arenkiel

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2020, Volume and Issue: unknown

Published: Dec. 2, 2020

Abstract Sensory perception underlies how we internalize and interact with the external world. In order to adapt changing circumstances interpret signals in a variety of contexts, sensation needs be reliable, but sensory input flexible. An important mediator this flexibility is top-down regulation from cholinergic basal forebrain. Basal forebrain projection neurons serve as pacemakers gatekeepers for downstream neural networks, modulating circuit activity across diverse neuronal populations. This control necessary cue detection, learning, memory, disproportionately disrupted neurodegenerative diseases associated cognitive decline. Intriguingly, signaling acts locally within sculpt output neurons. To determine local impacts pathways that participate regulation, sought define dynamics during motivated behavior learning. Towards this, utilized fiber photometry genetically encoded acetylcholine indicator GAChR2.0 temporal patterns olfactory-guided, behaviors We show reliably increased reward-seeking was strongly suppressed by reward delivery go/no-go, olfactory-cued discrimination task. The observed transient reduction tone mirrored suppression GABAergic activity. Together, these findings suggest changes rapidly reflect rewardseeking positive reinforcement impact

Language: Английский

Citations

1
Long-range GABAergic projections contribute to cortical feedback control of sensory processing DOI Creative Commons
Camille Mazo, Antoine Nissant, Soham Saha

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2020, Volume and Issue: unknown

Published: Dec. 20, 2020

Abstract Cortical sensory areas send excitatory projections back to earlier stage of processing. Here, we uncover for the first time existence a corticofugal inhibitory feedback between two areas, paralleling well-documented feedback. In olfactory system, reveal that subpopulation GABAergic neurons in anterior nucleus and piriform cortex target bulb. These long-range inputs synapse with both local output bulb neurons, mitral tufted cells. Optogenetic stimulation coupled vivo imaging network modeling showed activation these drives net subtractive inhibition spontaneous odor-evoked activity as well Further, cortical enhanced separation population odor responses cells, but not Targeted pharmacogenetic silencing axon terminals OB impaired discrimination similar mixtures. We propose here represents new circuit motif systems, involved refining processing perception.

Language: Английский

Citations

1