Wide‐ranging migration and destination of early olfactory placode‐derived neurons in chick embryos DOI
Momoko Miyakawa,

Shizuko Murakmai,

Yasuo Uchiyama

et al.

The Anatomical Record, Journal Year: 2022, Volume and Issue: 306(2), P. 298 - 310

Published: Sept. 15, 2022

Cell migration from the olfactory placode (OP) is a well-known phenomenon wherein various cell types, such as gonadotropin-releasing hormone (GnRH)-producing neurons, migrate toward telencephalon (TEL) during early embryonic development. However, spatial relationship between migratory cells and forebrain unclear. We examined development of whole-mount chick embryos to observe three-dimensional among OP-derived nerve (ON), TEL. Migratory neurons that express highly polysialylated neural adhesion molecule (PSA-NCAM) emerge OP spread over relatively wide TEL area at Hamburger Hamilton (HH) stage 17. Most form cellular cord pit rostral within HH18-20. The earliest axons epithelium (OE) were detected along this neuronal using DiI-labeling HH21. Furthermore, few PSA-NCAM-positive dispersed around lateral HH18. A long branch extending was transiently observed HH18-24. These results suggest novel route developmental stages. Following GFP vector introduction into HH13-15 embryos, labeled HH23 HH27. At HH36, in rostral-lateral TEL, including bulb (OB) region. GFP-labeled calretinin-positive OB, suggesting enter function interneurons OB.

Language: Английский

SPIRAL: integrating and aligning spatially resolved transcriptomics data across different experiments, conditions, and technologies DOI Creative Commons
Tiantian Guo, Zhiyuan Yuan,

Yan Pan

et al.

Genome biology, Journal Year: 2023, Volume and Issue: 24(1)

Published: Oct. 20, 2023

Abstract Properly integrating spatially resolved transcriptomics (SRT) generated from different batches into a unified gene-spatial coordinate system could enable the construction of comprehensive spatial transcriptome atlas. Here, we propose SPIRAL, consisting two consecutive modules: SPIRAL-integration, with graph domain adaptation-based data integration, and SPIRAL-alignment, cluster-aware optimal transport-based coordination alignment. We verify SPIRAL both synthetic real SRT datasets. By encoding correlations to gene expressions, SPIRAL-integration surpasses state-of-the-art methods in batch effect removal joint identification. aligning spots cluster-wise, SPIRAL-alignment achieves more accurate alignments than existing methods.

Language: Английский

Citations

25
Retinal Alterations Predict Early Prodromal Signs of Neurodegenerative Disease DOI Open Access
Fabio Casciano,

Enrico Zauli,

Claudio Celeghini

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1689 - 1689

Published: Jan. 30, 2024

Neurodegenerative diseases are an increasingly common group of that occur late in life with a significant impact on personal, family, and economic life. Among these, Alzheimer’s disease (AD) Parkinson’s (PD) the major disorders lead to mild severe cognitive physical impairment dementia. Interestingly, those may show onset prodromal symptoms early after middle age. Commonly, evaluation these neurodegenerative is based detection biomarkers, where functional structural magnetic resonance imaging (MRI) have shown central role revealing or phases, although it can be expensive, time-consuming, not always available. The aforementioned visual system due pathophysiological mechanisms shared between eye brain. In disease, α-synuclein deposition retinal cells, as well dopaminergic neurons substantia nigra, alters cortex function, resulting modifications field. Similarly, modified by neurofibrillary tangles neuritic amyloid β plaques typically seen brain, this reflect accumulation biomarkers retina during stages postmortem retinas AD patients. light, ophthalmic neurodegeneration could become cost-effective method for diagnosis diseases, overcoming limitations deep This analysis commonly used practice, interest has risen recent years. review will discuss relationship degeneration, highlighting how represent noninvasive straightforward diseases.

Language: Английский

Citations

12
Altered olfactory responses in Fmr1 KO mice DOI Creative Commons
Jan Tůma, Amtul-Noor Rana,

T Philip

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 23, 2025

Abstract Fragile X syndrome (FXS) is a neurodevelopmental disorder oftentimes associated with abnormal social behaviors and altered sensory responsiveness. It hypothesized that the inappropriate filtering of stimuli, including olfaction, can lead to aberrant behavior in FXS. However, previous studies investigating olfaction animal models FXS have shown inconsistent results. Here, we found Fmr1 knock-out (KO) mice, mouse model FXS, showed increased sniffing duration for non-social odors during their first exposure. Additionally, while wild-type (WT) males demonstrated differences behavioral patterns between KO did not show such distinction. We also spent significantly less time female urine odor compared WT males. Moreover, an volume olfactory bulb Overall, our findings suggest mice demonstrate atypical as well structural changes bulb.

Language: Английский

Citations

1
Restoration of Adult Neurogenesis by Intranasal Administration of Gangliosides GD3 and GM1 in The Olfactory Bulb of A53T Alpha-Synuclein-Expressing Parkinson’s-Disease Model Mice DOI
Takahiro Fuchigami, Yutaka Itokazu, John C. Morgan

et al.

Molecular Neurobiology, Journal Year: 2023, Volume and Issue: 60(6), P. 3329 - 3344

Published: Feb. 28, 2023

Language: Английский

Citations

15
Early and transitory hypoactivity and olfactory alterations after chronic atrazine exposure in female Sprague-Dawley rats DOI

Jonathan Sánchez-Yépez,

Triana Acevedo-Huergo,

María Soledad Mendoza-Trejo

et al.

NeuroToxicology, Journal Year: 2024, Volume and Issue: 101, P. 68 - 81

Published: Feb. 8, 2024

Language: Английский

Citations

3
The transcriptomic and spatial organization of telencephalic GABAergic neuronal types DOI Creative Commons
Cindy T. J. van Velthoven, Yuan Gao, Michael Kunst

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 18, 2024

The telencephalon of the mammalian brain comprises multiple regions and circuit pathways that play adaptive integrative roles in a variety functions. There is wide array GABAergic neurons telencephalon; they multitude functions, dysfunction these has been implicated diverse disorders. In this study, we conducted systematic in-depth analysis transcriptomic spatial organization neuronal types all mouse their developmental origins. This was accomplished by utilizing 611,423 single-cell transcriptomes from comprehensive high-resolution cell type atlas for adult whole have generated, supplemented with an additional RNA-sequencing dataset containing 99,438 high-quality collected pre- postnatal developing brain. We present hierarchically organized telencephalic taxonomy 7 classes, 52 subclasses, 284 supertypes, 1,051 clusters, as well corresponding 450 clusters across different ages. Detailed charting efforts reveal extraordinary complexity where relationships among reflect both locations Transcriptomically developmentally related can often be found distant indicating long-distance migration dispersion common characteristic nearly classes neurons. Additionally, find various dimensions discrete continuous variations are correlated gene expression gradients. Lastly, cortical, striatal some pallidal undergo extensive diversification, whereas septal most emerge simultaneously during embryonic stage limited diversification. Overall, serve foundational reference molecular, structural functional studies circuits entire community.

Language: Английский

Citations

3
Neurogenesis dynamics in the olfactory bulb: deciphering circuitry organization, function, and adaptive plasticity DOI Creative Commons
Moawiah M. Naffaa

Neural Regeneration Research, Journal Year: 2024, Volume and Issue: 20(6), P. 1565 - 1581

Published: June 26, 2024

Adult neurogenesis persists after birth in the subventricular zone, with new neurons migrating to granule cell layer and glomerular layers of olfactory bulb, where they integrate into existing circuitry as inhibitory interneurons. The generation these bulb supports both structural functional plasticity, aiding circuit remodeling triggered by memory learning processes. However, presence neurons, coupled cellular diversity within presents an ongoing challenge understanding its network organization function. Moreover, continuous integration plays a pivotal role regulating information processing. This adaptive process responds changes epithelial composition contributes formation memories modulating connectivity interacting intricately higher-order brain regions. adult functions remains topic debate. Nevertheless, functionality is linked cells around mitral tufted cells. organizational pattern significantly impacts output, behavior, synaptic which are crucial for perception memory. Additionally, this further shaped axon terminals originating from cortical subcortical Despite behaviors related olfaction, complex highly interconnected processes have not been comprehensively studied whole. Therefore, manuscript aims discuss our current explore how neural plasticity contribute enhancing adaptability system. These mechanisms thought support memory, potentially through increased complexity restructuring structures, well addition that aid adaptation. underscores importance employing precise methodologies elucidate specific roles amidst conflicting data varying experimental paradigms. Understanding essential gaining insights complexities function behavior.

Language: Английский

Citations

3
The neurobiological effects of senescence on dopaminergic system: A comprehensive review DOI
Gabriel S. Rocha, Marco Aurélio M. Freire, Karina Mary de Paiva

et al.

Journal of Chemical Neuroanatomy, Journal Year: 2024, Volume and Issue: 137, P. 102415 - 102415

Published: March 22, 2024

Language: Английский

Citations

2
Tropospheric ozone effect on olfactory perception and olfactory bulb dopaminergic interneuron excitability DOI Creative Commons
Angela Pignatelli, Mascia Benedusi, Mario Barbieri

et al.

NeuroToxicology, Journal Year: 2024, Volume and Issue: 104, P. 36 - 44

Published: July 14, 2024

Ozone (O3) forms in the Earth's atmosphere, both naturally and by reactions of man-made air pollutants. Deleterious effects O3 have been found respiratory system. Here, we examine whether alters olfactory behavior cellular properties For this purpose, mice were exposed to at a concentration highly polluted city [0.8 ppm], elicited social non-social odors habituation/dishabituation tests was assessed. In addition, electrical responses dopaminergic bulb (OB) neurons also evaluated. differentially compromises perception odors: it reduces Swiss Webster mice, while effect observed C57BL/6 J only for some odors. Additionally, reduced rate spontaneous spike firing periglomerular cells (PG-DA) OB. Because could reflect changes excitability and/or synaptic inputs, ability alter PG-DA activity tested together with cell membrane resistance, potential, rheobase chronaxie. Taken together, our data suggest affect perception.

Language: Английский

Citations

2
Characterization by Gene Expression Analysis of Two Groups of Dopaminergic Cells Isolated from the Mouse Olfactory Bulb DOI Creative Commons
Fabio Casciano, Nicoletta Bianchi,

Mirta Borin

et al.

Biology, Journal Year: 2023, Volume and Issue: 12(3), P. 367 - 367

Published: Feb. 25, 2023

The olfactory bulb (OB) is one of two regions the mammalian brain which undergo continuous neuronal replacement during adulthood. A significant fraction cells added in adulthood to bulbar circuitry constituted by dopaminergic (DA) neurons. We took advantage a peculiar property neurons transgenic mice expressing eGFP under tyrosine hydroxylase (TH) promoter: while DA located glomerular layer (GL) display full electrophysiological maturation, eGFP+ mitral (ML) show characteristics immature cells. In addition, they also lower fluorescence intensity, possibly reflecting different degrees maturation. To investigate whether this difference maturation might be confirmed at gene expression level, we used fluorescence-activated cell sorting technique on enzymatically dissociated OB. were divided into groups based their level fluorescence, corresponding ML and fully mature from GL. Semiquantitative real-time PCR was performed detect genes linked degree showed that indeed low are Our method can further explore differences between these

Language: Английский

Citations

4