Functional characterisation of neuropeptides that act as ligands for both calcitonin-type and pigment-dispersing factor-type receptors in a deuterostome DOI Open Access
Cong Xiao, Huachen Liu, Lihua Liu

et al.

Published: Sept. 23, 2024

The calcitonin (CT) family of related peptides exert their diverse physiological effects in mammals via two G-protein coupled receptors, CTR and the CTR-like receptor CLR. Analysis phylogenetic distribution CT-type signaling has revealed occurrence CTR/CLR-type proteins deuterostome protostome invertebrates. Furthermore, experimental studies have that Drosophila melanogaster CT-like peptide DH 31 can act as a ligand for pigment-dispersing factor (PDF) receptor. Here we investigated mechanisms functions neuropeptides invertebrate, sea cucumber Apostichopus japonicus (phylum Echinodermata). In A. japonicus, single gene encodes (AjCT1 AjCT2), both which ligands (AjCTR) PDF-type receptors (AjPDFR1, AjPDFR2), but with differential activation downstream cAMP/PKA, Gαq/Ca 2+ /PKC ERK1/2 pathways. expression encoding AjCT1 AjCT2 transcripts variety organ systems, highest circumoral nervous system. vitro pharmacological experiments and/or cause dose-dependent relaxation longitudinal body wall muscle intestine preparations. vivo loss-of-function tests potential role promoting feeding growth . This is first study to obtain evidence deuterostome. because economic importance foodstuff, discovery regulators this species may provide basis practical applications aquaculture.

Language: Английский

Identification and characterization of neuropeptides in sea urchin Strongylocentrotus intermedius DOI
Qianqian Li,

Mingya Zhang,

K. Liu

et al.

General and Comparative Endocrinology, Journal Year: 2025, Volume and Issue: unknown, P. 114716 - 114716

Published: March 1, 2025

Language: Английский

Citations

0
Localization of relaxin‐like gonad‐stimulating peptide expression in starfish reveals the gonoducts as a source for its role as a regulator of spawning DOI Creative Commons

Yuling Feng,

Vı́ctor M. González, Ming Lin

et al.

The Journal of Comparative Neurology, Journal Year: 2023, Volume and Issue: 531(13), P. 1299 - 1316

Published: May 22, 2023

Abstract Oocyte maturation and gamete release (spawning) in starfish are triggered by relaxin‐like gonad‐stimulating peptide (RGP), a neuropeptide that was first isolated from the radial nerve cords of these animals. Hitherto, it has generally been assumed source RGP triggers spawning physiologically. To investigate other sources RGP, here we report comprehensive anatomical analysis its expression, using both situ hybridization immunohistochemistry to map precursor transcripts respectively, Asterias rubens . Cells expressing were revealed ectoneural epithelium circumoral ring, arm tips, tube feet, cardiac stomach, pyloric and, most notably, gonoducts. Using specific antibodies A. immunostaining cells and/or fibers region terminal tentacle tip‐associated structures, body wall, peristomial membrane, esophagus, caeca, Our discovery is expressed gonoducts proximal gonadotropic site action gonads important because provides new perspective on how may act as gonadotropin starfish. Thus, hypothesize starfish, while produced parts regulate physiological/behavioral processes.

Language: Английский

Citations

9
Relaxin-like Gonad-Stimulating Peptides in Asteroidea DOI Creative Commons
Masatoshi Mita

Biomolecules, Journal Year: 2023, Volume and Issue: 13(5), P. 781 - 781

Published: April 30, 2023

Starfish relaxin-like gonad-stimulating peptide (RGP) is the first identified hormone with gonadotropin-like activity in invertebrates. RGP a heterodimeric peptide, comprising A and B chains disulfide cross-linkages. Although had been named substance (GSS), purified member of relaxin-type family. Thus, GSS was renamed as RGP. The cDNA encodes not only chains, but also signal C-peptides. After

Language: Английский

Citations

8
A Putative Role of Vasopressin/Oxytocin-Type Neuropeptide in Osmoregulation and Feeding Inhibition of Apostichopus japonicus DOI Open Access
Cong Xiao, Huachen Liu,

Yingqiu Zheng

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(18), P. 14358 - 14358

Published: Sept. 20, 2023

Vasopressin/oxytocin (VP/OT)-type neuropeptide is an ancient neurophysin-associated and has been intensively studied to be involved in multiple physiological processes protostomian deuterostome vertebrates. However, little known about the functions of VP/OT-type invertebrates especially echinoderms. Here, we firstly report signaling important economic species, Apostichopus japonicus, which widely cultured Asia, with high nutritional medicinal values. Molecular characterization analysis holotocin its precursor revealed highly conserved features VP/OT family. The candidate receptor for (AjHOR) was confirmed able activate via cAMP-PKA possible Ca2+-PKC pathway, further activated downstream ERK1/2 cascade. Holotocin expression profile showed that they were mainly concentrated circumoral nerve ring. Furthermore, vitro pharmacological experiments demonstrated caused contractile responses preparations from A. japonicus. And vivo functional studies indicated short-term injection resulted body bloat long-term reduced mass, suggesting potential roles osmoregulation feeding co-inhibition holotocin-CCK. Our findings provided a comprehensive description AjHOR-holotocin signaling, inhibition by controlling muscle contractions.

Language: Английский

Citations

7
Endocrine regulation of reproductive biology in echinoderms: An evolutionary perspective from closest marine invertebrate relatives to chordates DOI
Yixin Wang, Xinghai Liu,

Yingqiu Zheng

et al.

Molecular and Cellular Endocrinology, Journal Year: 2023, Volume and Issue: 580, P. 112105 - 112105

Published: Nov. 10, 2023

Language: Английский

Citations

7
A feather star is born: embryonic development and nervous system organization in the crinoid Antedon mediterranea DOI Creative Commons
Silvia Mercurio, Giacomo Gattoni, Giorgio Scarı̀

et al.

Open Biology, Journal Year: 2024, Volume and Issue: 14(8)

Published: Aug. 1, 2024

Crinoids belong to the Echinodermata, marine invertebrates with a highly derived adult pentaradial body plan. As sister group all other extant echinoderms, crinoids occupy key phylogenetic position explore evolutionary history of whole phylum. However, their development remains understudied compared that echinoderms. Therefore, aim here was establish Mediterranean feather star ( Antedon mediterranea ) as an experimental system for developmental biology. We first set up method culturing embryos in vitro and defined standardized staging this species. then optimized protocols characterize morphological molecular main structures Focusing on nervous system, we showed larval apical organ includes serotonergic, GABAergic glutamatergic neurons, which develop within conserved anterior signature. described composition early post-metamorphic revealed it has These results further our knowledge crinoid provide new techniques investigate embryogenesis. This will pave way inclusion comparative studies addressing origin echinoderm plan diversification deuterostomes.

Language: Английский

Citations

2
A feather star is born: embryonic development and nervous system organization in the crinoidAntedon mediterranea DOI Creative Commons
Silvia Mercurio, Giacomo Gattoni, Giorgio Scarı̀

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 30, 2024

Abstract Background Crinoids belong to the phylum Echinodermata, marine invertebrates with a highly derived pentaradial body plan. As only living members of Pelmatozoa, sister group other extant echinoderms, crinoids are in key phylogenetic position reconstruct evolutionary history this phylum. However, development has been scarcely investigated, limiting their potential for comparative studies. Many difficult collect wild and embryo manipulation is challenging. Conversely, Mediterranean feather star Antedon mediterranea can be found shallow waters used experimental studies, most notably investigate regeneration. Results The aim here was establish A. as an system developmental biology. To accomplish this, we set up method culturing embryos vitro from zygote hatching larva stage that allowed us define timeline standardized staging species. We then optimized protocols characterize main structures plan, using combination microscopy techniques whole mount immunohistochemistry situ hybridization chain reaction. Focusing on nervous system, show larval apical organ includes serotonergic, GABAergic glutamatergic neurons form under influence conserved anterior molecular signature. neural plexus instead composed develops during formation ciliary bands. Larval disappear at metamorphosis, ectoneural entoneural components adult develop early post-metamorphic stages. Furthermore, oral ectoderm contains acquires “anterior” signature expressing Six3/6 Lhx2/9 orthologs. Conclusions Our results deepen our knowledge crinoid provide new embryogenesis, promoting use This turn will pave way inclusion studies understand origin echinoderm plan clarify many unanswered questions deuterostome evolution.

Language: Английский

Citations

1
Research Progress on Starfish Outbreaks and Their Prevention and Utilization DOI Open Access
Liang Qu,

Yongxin Sun,

Chong Zhao

et al.

Published: June 27, 2024

Starfish are keystone species as predators in benthic ecosystems, but when population outbreaks occur this can have devastating consequences ecologically. Furthermore, starfish and invasions adverse impact economically by impacting shellfish aquaculture. Addressing the imperative need to proactively mitigate requires comprehensive research on their behavior underlying mechanisms of outbreaks. This review scrutinizes historical patterns among diverse across various regions, delineates factors contributing proliferation Asterias amurensis Chinese waters, articulates preventive remedial strategies, outlines potential for sustainable utilization starfish.

Language: Английский

Citations

1
Research Progress on Starfish Outbreaks and Their Prevention and Utilization: Lessons from Northern China DOI Creative Commons
Liang Qu,

Yongxin Sun,

Chong Zhao

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(7), P. 537 - 537

Published: July 17, 2024

Starfish are keystone species as predators in benthic ecosystems, but when population outbreaks occur, this can have devastating consequences ecologically. Furthermore, starfish and invasions adverse impact economically by impacting shellfish aquaculture. For example, an infestation of Qingdao led to a 50% reduction sea cucumber production 80% scallop production, resulting economic loss approximately RMB 100 million oyster other industries. Addressing the imperative need proactively mitigate requires comprehensive research on their behavior underlying mechanisms outbreaks. This review scrutinizes historical patterns among diverse across various regions, delineates factors contributing proliferation

Language: Английский

Citations

1
Functional characterisation of neuropeptides that act as ligands for both calcitonin-type and pigment-dispersing factor-type receptors in a deuterostome DOI Open Access
Cong Xiao, Huachen Liu, Lihua Liu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Abstract The calcitonin (CT) family of related peptides exert their diverse physiological effects in mammals via two G-protein coupled receptors, CTR and the CTR-like receptor CLR. Analysis phylogenetic distribution CT-type signaling has revealed occurrence CTR/CLR-type proteins deuterostome protostome invertebrates. Furthermore, experimental studies have that Drosophila melanogaster CT-like peptide DH 31 can act as a ligand for pigment-dispersing factor (PDF) receptor. Here we investigated mechanisms functions neuropeptides invertebrate, sea cucumber Apostichopus japonicus (phylum Echinodermata). In A. japonicus, single gene encodes (AjCT1 AjCT2), both which ligands (AjCTR) PDF-type receptors (AjPDFR1, AjPDFR2), but with differential activation downstream cAMP/PKA, Gαq/Ca 2+ /PKC ERK1/2 pathways. expression encoding AjCT1 AjCT2 transcripts variety organ systems, highest circumoral nervous system. vitro pharmacological experiments and/or cause dose-dependent relaxation longitudinal body wall muscle intestine preparations. vivo loss-of-function tests potential role promoting feeding growth . This is first study to obtain evidence deuterostome. because economic importance foodstuff, discovery regulators this species may provide basis practical applications aquaculture. Graphical abstract A schematic showing proposed molecular by regulate are represented purple yellow circles, respectively. For cell signaling, black arrows indicate process activated, whilst pink arrow indicates activated AjCT1/AjPDFR1, AjCT2/AjPDFR1 AjCT2/AjPDFR2

Language: Английский

Citations

1