Microfluidic systems for modeling digestive cancer: a review of recent progress

Biomedical Physics & Engineering Express, Journal Year: 2024, Volume and Issue: 10(5), P. 052002 - 052002

Published: Aug. 14, 2024

Abstract Purpose . This review aims to highlight current improvements in microfluidic devices designed for digestive cancer simulation. The emphasizes the use of multicellular 3D tissue engineering models understand complicated biology tumor microenvironment (TME) and progression. purpose is develop oncology research improve patients’ lives. Methods analyzes recent on mimicking cancer. It uses tissue-engineered devices, notably organs a chip (OOC), simulate human organ function lab. Cell cultivation modern three-dimensional hydrogel platforms allows precise geometry, biological components, physiological qualities. novel methodologies, key findings, technical progress explain this field’s advances. Results study discusses advances Micro systems with are emphasized. These capture complex biochemical gradients, niche variables, dynamic cell–cell interactions (TME). reveal stomach progression by duplicating TME. Recent discoveries technology have improved our understanding gut biology, as shown review. Conclusion Microfluidic play crucial role modeling furthering research. could transform drug development treatment revealing provides complete summary suggests future field professionals. review’s major goal further medical

Language: Английский

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Helicobacter pylori and gastric cancer: current insights and nanoparticle-based interventions

RSC Advances, Journal Year: 2025, Volume and Issue: 15(7), P. 5558 - 5570

Published: Jan. 1, 2025

Background: H. pylori is recognized as one of the main causes gastric cancer, and this type cancer considered leading diseases causing deaths all over world.

Language: Английский

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Cyclophilin A Inhibitors Suppress Proliferation and Induce Apoptosis of MKN45 Gastric Cancer Stem-like Cells by Regulating CypA/CD147-Mediated Signaling Pathway

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(5), P. 4734 - 4734

Published: March 1, 2023

Gastric cancer stem cells (GCSCs) are a subgroup of gastric (GC) with high self-renewal and multi-lineage differentiation abilities that lead to tumor initiation, metastasis, drug resistance, relapse. Therefore, the eradication GCSCs can contribute effective treatment advanced or metastatic GC. In our previous study, compound 9 (C9), novel derivative nargenicin A1, was identified as potential natural anticancer agent specifically targeted cyclophilin A (CypA). However, its therapeutic effect molecular mechanisms action on GCSC growth have not been assessed. this we investigated effects CypA inhibitors, including C9 cyclosporin (CsA), MKN45-derived GCSCs. Compound CsA effectively suppressed cell proliferation by inducing cycle arrest at G0/G1 phase promoted apoptosis activating caspase cascade in MKN45 addition, potently inhibited GCSC-grafted chick embryo chorioallantoic membrane (CAM) model. Furthermore, two compounds significantly decreased protein expression key markers CD133, CD44, integrin α6, Sox2, Oct4, Nanog. Notably, activities were associated regulation CypA/CD147-mediated AKT mitogen-activated kinase (MAPK) signaling pathways. Collectively, findings suggest inhibitors could be agents used combat targeting CypA/CD147 axis.

Language: Английский

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The hypoxia-inducible factor-1α in stemness and resistance to chemotherapy in gastric cancer: Future directions for therapeutic targeting

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: Feb. 8, 2023

Hypoxia-inducible factor-1α (HIF-1α) is a crucial mediator of intra-tumoral heterogeneity, tumor progression, and unresponsiveness to therapy in tumors with hypoxia. Gastric tumors, one the most aggressive clinic, are highly enriched hypoxic niches, degree hypoxia strongly correlated poor survival gastric cancer patients. Stemness chemoresistance two root causes patient outcomes. Based on pivotal role HIF-1α stemness cancer, interest identifying critical molecular targets strategies for surpassing action expanding. Despite that, understanding induced signaling far from complete, development efficacious inhibitors bears various challenges. Hence, here we review mechanisms by which stimulates clinical efforts challenges translate anti-HIF-1α into clinic.

Language: Английский

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The potential therapeutic effects of Galbanic acid on cancer

Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 248, P. 154686 - 154686

Published: July 13, 2023

Language: Английский

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RNF220 promotes gastric cancer growth and stemness via modulating the USP22/wnt/β-catenin pathway

Tissue and Cell, Journal Year: 2023, Volume and Issue: 83, P. 102123 - 102123

Published: June 1, 2023

Language: Английский

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Strategies for the Isolation and Identification of Gastric Cancer Stem Cells

Stem Cells International, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

Gastric cancer stem cells (GCSCs) originate from both gastric adult and bone marrow are conspicuously present within the histological milieu of tissue. GCSCs play pivotal multifaceted roles in initiation, progression, recurrence cancer. Hence, characterization not only facilitates precise target identification for prospective therapeutic interventions but also has significant implications targeted therapy prognosis The prevailing techniques GCSC purification involve their isolation using surface‐specific cell markers, such as those identified by flow cytometry immunomagnetic bead sorting techniques. In addition, vitro culture side‐population integral methods this context. This review discusses surface biomarkers, techniques, GCSCs, well role treatment

Language: Английский

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Preparation and Identification of a Fluorescent Probe with CsPbBr3 Perovskite Quantum Dots and CD44v6 specific peptide for gastric cancer imaging

Nanotechnology, Journal Year: 2024, Volume and Issue: 36(2), P. 02LT02 - 02LT02

Published: Oct. 15, 2024

Since the sensitivity and accuracy of traditional detection for early gastric cancer diagnosis are still insufficient, it is significant to continuously optimize optical molecular imaging technology based on an endoscopic platform. The signal intensity stability chemical fluorescent dyes low, which hinders clinical application technology. This work developed a probe perovskite quantum dots (PQDs) peptide ligands. By utilizing CsPbBr

Language: Английский

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FTO Promotes the Stemness of Gastric Cancer Cells

DNA and Cell Biology, Journal Year: 2023, Volume and Issue: 42(7), P. 411 - 420

Published: May 25, 2023

The full name of the FTO gene is fat mass and obesity-associated gene. In recent years, it has also been found that involved in m6A demethylation regulates progression multiple cancers, including gastric cancer. cancer stem cell theory argues cells are key factors metastasis, inhibiting expression stemness genes a good method to inhibit metastasis Currently, role regulating still unclear. By analyzing public databases, was discovered increased cancer, high associated with poor prognosis patients After were isolated, protein cells; reduced after knockdown; subcutaneous tumors nude mice smaller than those control group enhanced overexpressed by plasmid. reviewing additional literature experimental validation, we SOX2 may be factor which promotes cells. Therefore, concluded could promote cells, targeting potential therapeutic approach for metastatic CTR number: TOP-IACUC-2021-0123.

Language: Английский

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Blockade of Src signaling prevented stemness gene expression and proliferation of patient-derived gastric cancer stem cells

Tzu Chi Medical Journal, Journal Year: 2024, Volume and Issue: 37(1), P. 65 - 71

Published: Sept. 30, 2024

Gastric cancer (GC) is one of the most malignant tumors. Mounting studies highlighted gastric stem cells (GCSCs) were responsible for failure treatment due to recurrence and drug resistance advanced GC. However, targeted therapy against GCSC improving GC prognosis suffered from lack suitable models molecular targets in terms personalized medicine. To address this issue, two patient-derived cell lines SD209 SD292 with (CSCs) such as phenotype isolated establishing aiming at critical metastatic signaling The primary GCSCs established parts tissues characterization (SCs) both cellular levels. Western blot Immunohistochemistry (IHC) performed identifying deregulated tissue. Immunofluorescence was used analyzing proliferating SC markers attached on fibroblast. Acridine orange propidium iodide analyses survival suspensions. In culture environments SD292, a lot mesenchymal fibroblasts spread crowd together which clumps, suspected GCSC, firmly attached. IHC analysis, stemness genes CD44 Ep-CAM increased tumor SD209, whereas Nanog-1 octamer-binding transcription factor 3 (OCT-3) that SD292. By immunofluorescent analysis proliferation marker Ki67, growth found be reduced by dasatinib, inhibitor Src kinase whose activity upregulated GCs. Dasatinib also suppressed expression OCT-3 fibroblasts. This study may provide base GCSCs/GCs progression future preclinical/clinical settings.

Language: Английский

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