Low-intensity pulsed ultrasound promotes the osteogenesis of mechanical force-treated periodontal ligament cells via Piezo1

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: April 17, 2024

Background Low-intensity pulsed ultrasound (LIPUS) can accelerate tooth movement and preserve bone integrity during orthodontic treatment. However, the mechanisms by which LIPUS affects tissue remodeling (OTM) remain unclear. Periodontal ligament cells (PDLCs) are pivotal in maintaining periodontal equilibrium when subjected to mechanical stimuli. One notable mechano-sensitive ion channel, Piezo1, modulate cellular function response cues. This study aimed elucidate involvement of Piezo1 osteogenic force-treated PDLCs stimulated LIPUS. Method After establishing rat OTM models, was used stimulate rats locally. distance alveolar density were assessed using micro-computed tomography, histological analyses included hematoxylin eosin staining, tartrate-resistant acid phosphatase staining immunohistochemical staining. GsMTx4 Yoda1 respectively utilized for functional inhibition activation experiments rats. We isolated human (hPDLCs) vitro evaluated effects on differentiation hPDLCs real-time quantitative PCR, Western blot, alkaline alizarin red Small interfering RNA employed validate role this process. Results promoted osteoclast accelerated Furthermore, alleviated resorption under pressure enhanced osteogenesis both vivo downregulating expression. Subsequent administration siPIEZO1 transfection attenuated inhibitory effect pressure, whereas efficacy partially mitigated. treatment inhibited hPDLCs, resulting reduced expression Collagen Ⅰα1 osteocalcin ligament. able counteract these effects. Conclusion research unveils that promotes via Piezo1.

Language: Английский

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Mechanobiology of Dental Pulp Cells

Cells, Journal Year: 2024, Volume and Issue: 13(5), P. 375 - 375

Published: Feb. 21, 2024

The dental pulp is the inner part of tooth responsible for properly functioning during its lifespan. Apart from very big biological heterogeneity cells, microenvironments differ a lot in context mechanical properties-ranging 5.5 kPa to around 100 GPa dentin and enamel. This physical complexity plays key role physiology turn, great target variety therapeutic approaches. First all, mechanisms are crucial pain propagation process surface nerves inside pulp. On other hand, modulation environment affects cells thus important regenerative medicine. In present review, we describe physiological significance biomechanical processes pathology Moreover, couple those phenomena with recent advances fields bioengineering pharmacology aiming control reduce pain, enhance differentiation into desired lineages. reviewed literature shows progress topic pulp-although mainly vitro. few positions, it leaves gap necessary filling studies providing vivo.

Language: Английский

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The Role of the Piezo1 Mechanosensitive Channel in the Musculoskeletal System

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(7), P. 6513 - 6513

Published: March 30, 2023

Since the recent discovery of mechanosensitive Piezo1 channels, many studies have addressed role channel in various physiological or even pathological processes different organs. Although number on their effects musculoskeletal system is constantly increasing, we are still far from a precise understanding. In this review, knowledge available so regarding summarized, reviewing results achieved field skeletal muscles, bones, joints and cartilage, tendons ligaments, as well intervertebral discs.

Language: Английский

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The anatomy, neurophysiology, and cellular mechanisms of intradental sensation

Frontiers in Pain Research, Journal Year: 2024, Volume and Issue: 5

Published: March 25, 2024

Somatosensory innervation of the oral cavity enables detection a range environmental stimuli including minute and noxious mechanical forces. The trigeminal sensory neurons underlie sensation originating from tooth. Prior work has provided important physiological molecular characterization dental pulp innervation. Clinical experiences have informed our conception consequence activating these neurons. However, biological role within tooth is yet to be defined. Recent transcriptomic data, combined with mouse genetic tools, capacity provide cell-type resolution for behavioral function pulp-innervating Importantly, tools can applied determine neuronal origin acute pain that coincides damage as well stemming tissue inflammation (i.e., pulpitis) toward developing treatment strategies aimed at relieving distinct forms pain.

Language: Английский

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PIEZO1 Ion Channels Mediate Mechanotransduction in Odontoblasts

Journal of Endodontics, Journal Year: 2022, Volume and Issue: 48(6), P. 749 - 758

Published: Feb. 25, 2022

IntroductionOdontoblasts, terminally differentiated dentin-forming cells with their processes that penetrate into dentin, have been considered potential sensory cells. Current research suggests odontoblasts sense external stimuli and transmit pain signals. PIEZO1, as a specific mechanically activated ion channel, may play an important role in mechanical transduction odontoblasts. In this study, we devoted to investigating the functions underlying molecular mechanisms of PIEZO1 channels odontoblast mechanotransduction.MethodsHuman dental pulp stem were cultured vitro induced differentiate odontoblast-like (OLCs). The expression protein pulp, cells, OLCs was detected by immunohistochemistry or immunofluorescence. sensitivity constructed fluid shear stress model examined calcium fluorescence intensity. A single-cell stimulation used detect electrophysiological properties OLCs. Yoda1 (a PIEZO1-specific agonist), GsMTx4 antagonist), non–calcium extracellular solution utilized confirm mechanotransduction both assays. amount ATP released measured under GsMTx4. Rat trigeminal ganglion neurons whole-cell patch-clamp recording stimulation.ResultsPIEZO1 positively expressed odontoblastic bodies but weakly After treatment shearing Yoda1, intensity intracellular ions increased rapidly did not noticeably change after solution. When applied OLCs, evoked inward currents recorded electrophysiology. current inactivation became slower treatment, these almost completely disappeared addition significantly stimulation, while reversed release ATP. Whole-cell detection showed slow frequency action potentials neurons.ConclusionsTaken together, findings indicated fast via application signals adjacent Thus, mediate various pathophysiological conditions dentin.

Language: Английский

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Piezo1-pannexin-1-P2X3 axis in odontoblasts and neurons mediates sensory transduction in dentinal sensitivity

Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 13

Published: Dec. 14, 2022

According to the "hydrodynamic theory," dentinal pain or sensitivity is caused by fluid movement following application of various stimuli dentin surface. Recent convergent evidence in Vitro has shown that plasma membrane deformation, mimicking movement, activates mechanosensitive transient receptor potential (TRP)/Piezo channels odontoblasts, with Ca2+ signal eliciting release ATP from pannexin-1 (PANX-1). The released P2X3 receptor, which generates and propagates action potentials intradental Aδ afferent neurons. Thus, odontoblasts act as sensory cells, odontoblast-neuron communication established TRP/Piezo channel-PANX-1-P2X3 complex may describe mechanism transduction sequence for sensitivity. To determine whether acting receptors are essential generating pain, we evaluated nociceptive scores analyzing behaviors evoked conscious Wistar rats Cre-mediated transgenic mouse models. In dentin-exposed group, treatment a bonding agent on surface, well systemic administration A-317491 (P2X3 antagonist), mefloquine 10PANX (non-selective selective PANX-1 antagonists), GsMTx-4 (selective Piezo1 channel HC-030031 TRPA1 but not HC-070 TRPC5 significantly reduced cold water (0.1 ml) stimulation exposed surface incisors compared without local treatment. When applied lower first molar, A-317491, 10PANX, were those Dentin-exposed mice, somatic odontoblast-specific depletion, also showed significant reduction did show any type cell deletion, including odontoblasts. odontoblast-eliminated receptor-positive A-neurons morphologically intact. These results indicate neurotransmission between neurons mediated Piezo1/TRPA1-pannexin-1-P2X3 axis necessary development pain. addition, generate mechanosensory cells.

Language: Английский

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Mechanosensitive Piezo1 and Piezo2 ion channels in craniofacial development and dentistry: Recent advances and prospects

Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 21, 2022

Mechanical forces play important roles in many biological processes and there is increasing interest understanding of these roles. Mechanotransduction the process by which mechanical stimuli are converted to biochemical signals through specific mechanisms, this results activation downstream signaling pathways with effects on cell behaviors. This review systematically summarizes current mechanosensitive Piezo1 Piezo2 ion channels craniofacial bone, tooth, periodontal tissue, presenting latest relevant evidence implications for potential treatments managements dental orofacial diseases deformities. The widely expressed various cells tissues have essential functions mechanosensation mechanotransduction. These an active role physiological pathological processes, such as growth development, mechano-stimulated bone homeostasis mediation inflammatory responses. Emerging indicates expression tissue-derived stem suggests that they function sensation transduction, dentin mineralization remodeling modulate orthodontic tooth movement.

Language: Английский

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The Migration and the Fate of Dental Pulp Stem Cells

Biology, Journal Year: 2023, Volume and Issue: 12(5), P. 742 - 742

Published: May 19, 2023

Human dental pulp stem cells (hDPSCs) are adult mesenchymal (MSCs) obtained from and derived the neural crest. They can differentiate into odontoblasts, osteoblasts, chondrocytes, adipocytes nerve cells, they play a role in tissue repair regeneration. In fact, DPSCs, depending on microenvironmental signals, odontoblasts regenerate dentin or, when transplanted, replace/repair damaged neurons. Cell homing depends recruitment migration, it is more effective safer than cell transplantation. However, main limitations of poor migration MSCs limited information we have regulatory mechanism direct differentiation MSCs. Different isolation methods used to recover DPSCs yield different types. To date, most studies use enzymatic method, which prevents observation migration. Instead, explant method allows for single that migrate at two times and, therefore, could fates, example, self-renewal. amoeboid modes with formation lamellipodia, filopodia blebs, biochemical biophysical signals microenvironment. Here, present current knowledge possible intriguing particular attention cues mechanosensing properties, fate DPSCs.

Language: Английский

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Dual action of Dooku1 on PIEZO1 channel in human red blood cells

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: July 10, 2023

PIEZO1 is a mechanosensitive non-selective cation channel, present in many cell types including Red Blood Cells (RBCs). Together with the Gárdos forms RBCs tandem that participates rapid adjustment of volume. The pharmacology allowing functional studies roles has only recently been developed, Yoda1 as widely used agonist. In 2018, analogues were step towards an improved understanding and functions. Among these, Dooku1 was most promising antagonist Yoda1-induced effects, without having any ability to activate channels. Since then, various antagonize effects. study using RBCs, shows apparent IC 50 on effects 90.7 µM, one order magnitude above previously reported data other types. Unexpectedly, it able, by itself , produce entry calcium sufficient trigger channel activation. Moreover, evoked rise intracellular sodium concentrations, suggesting targets channel. abolished upon GsMTx4, known blocker, indicating likely PIEZO1. Our observations lead conclusion behaves agonist RBC membrane, similarly but lower potency. Taken together, these results show must be interpreted care especially due unique characteristics membrane associated cytoskeleton.

Language: Английский

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Dentin Mechanobiology: Bridging the Gap between Architecture and Function

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5642 - 5642

Published: May 22, 2024

It is remarkable how teeth maintain their healthy condition under exceptionally high levels of mechanical loading. This suggests the presence inherent adaptation mechanisms within structure to counter constant stress. Dentin, situated between enamel and pulp, plays a crucial role in mechanically supporting tooth function. Its intermediate stiffness viscoelastic properties, attributed its mineralized, nanofibrous extracellular matrix, provide flexibility, strength, rigidity, enabling it withstand loading without fracturing. Moreover, dentin’s unique architectural features, such as odontoblast processes dentinal tubules spatial compartmentalization odontoblasts dentin sensory neurons contribute distinctive perception external stimuli while acting defensive barrier for dentin-pulp complex. Since architecture governs functions nociception repair response stimuli, understanding mechanobiology developing treatments pain management dentin-associated diseases regeneration. review discusses physical features regulate mechano-sensing, focusing on mechano-sensitive ion channels. Additionally, we explore advanced vitro platforms that mimic providing deeper insights into fundamental mechanobiological phenomena laying groundwork effective mechano-therapeutic strategies diseases.

Language: Английский

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