Piezo1‐mediated M2 macrophage mechanotransduction enhances bone formation through secretion and activation of transforming growth factor‐β1

Cell Proliferation, Journal Year: 2023, Volume and Issue: 56(9)

Published: March 7, 2023

Macrophages are multifunctional immune system cells that essential for the mechanical stimulation-induced control of metabolism. Piezo1 is a non-selective calcium channel expressed in multifarious tissues to convey signals. Here, cellular model tension was used study effect stretch on phenotypic transformation macrophages and its mechanism. An indirect co-culture explore macrophage activation bone marrow mesenchymal stem (BMSCs), treadmill running validate mechanism vivo vitro studies. p53 acetylated deacetylated by as result strain being detected Piezo1. This process able polarize towards M2 secretes transforming growth factor-beta (TGF-β1), which subsequently stimulates BMSCs migration, proliferation osteogenic differentiation. Knockdown inhibits conversion reparative phenotype, thereby affecting remodelling. Blockade TGF-β I, II receptors significantly reduced exercise-increased mass mice. In conclusion, we showed causes influx, deacetylation, polarization TGF-β1 release through These events support BMSC osteogenesis.

Language: Английский

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Piezo1 channel‐mediated Ca²⁺ signaling inhibits lipopolysaccharide‐induced activation of the NF‐κB inflammatory signaling pathway and generation of TNF‐α and IL‐6 in microglial cells

Glia, Journal Year: 2022, Volume and Issue: 71(4), P. 848 - 865

Published: Nov. 29, 2022

Microglial cells are crucial in maintaining central nervous system (CNS) homeostasis and mediating CNS disease pathogenesis. Increasing evidence supports that alterations the mechanical properties of microenvironments influence glial cell phenotypes, but mechanisms regulating microglial function remain elusive. Here, we examined mechanosensitive Piezo1 channel cells, particularly, how activation regulates pro-inflammatory production cytokines, using BV2 primary cells. expression was detected both at mRNA protein levels. Application activator Yoda1 induced Ca2+ flux to increase intracellular concentration reduced by treatment with ruthenium red, a inhibitor, or Piezo1-specific siRNA, supporting functions as surface -permeable channel. Priming lipopolysaccharide (LPS) TNF-α IL-6, which were inhibited Yoda1. Furthermore, LPS priming ERK, p38 MAPKs, NF-κB. LPS-induced NF-κB, not ERK p38, Yoda1-induced inhibition blunted siRNA-mediated depletion and, furthermore, BAPTA-AM prevent increase. Collectively, our results support downregulates especially initiating signaling inhibit NF-κB inflammatory pathway. These findings reveal previously unrecognized mechanism function, raising an interesting perspective on targeting this molecular alleviate neuroinflammation associated pathologies.

Language: Английский

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Mechanobiology of Dental Pulp Cells

Cells, Journal Year: 2024, Volume and Issue: 13(5), P. 375 - 375

Published: Feb. 21, 2024

The dental pulp is the inner part of tooth responsible for properly functioning during its lifespan. Apart from very big biological heterogeneity cells, microenvironments differ a lot in context mechanical properties-ranging 5.5 kPa to around 100 GPa dentin and enamel. This physical complexity plays key role physiology turn, great target variety therapeutic approaches. First all, mechanisms are crucial pain propagation process surface nerves inside pulp. On other hand, modulation environment affects cells thus important regenerative medicine. In present review, we describe physiological significance biomechanical processes pathology Moreover, couple those phenomena with recent advances fields bioengineering pharmacology aiming control reduce pain, enhance differentiation into desired lineages. reviewed literature shows progress topic pulp-although mainly vitro. few positions, it leaves gap necessary filling studies providing vivo.

Language: Английский

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Mechanosensitive Piezo1 and Piezo2 ion channels in craniofacial development and dentistry: Recent advances and prospects

Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 21, 2022

Mechanical forces play important roles in many biological processes and there is increasing interest understanding of these roles. Mechanotransduction the process by which mechanical stimuli are converted to biochemical signals through specific mechanisms, this results activation downstream signaling pathways with effects on cell behaviors. This review systematically summarizes current mechanosensitive Piezo1 Piezo2 ion channels craniofacial bone, tooth, periodontal tissue, presenting latest relevant evidence implications for potential treatments managements dental orofacial diseases deformities. The widely expressed various cells tissues have essential functions mechanosensation mechanotransduction. These an active role physiological pathological processes, such as growth development, mechano-stimulated bone homeostasis mediation inflammatory responses. Emerging indicates expression tissue-derived stem suggests that they function sensation transduction, dentin mineralization remodeling modulate orthodontic tooth movement.

Language: Английский

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Dentin Mechanobiology: Bridging the Gap between Architecture and Function

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5642 - 5642

Published: May 22, 2024

It is remarkable how teeth maintain their healthy condition under exceptionally high levels of mechanical loading. This suggests the presence inherent adaptation mechanisms within structure to counter constant stress. Dentin, situated between enamel and pulp, plays a crucial role in mechanically supporting tooth function. Its intermediate stiffness viscoelastic properties, attributed its mineralized, nanofibrous extracellular matrix, provide flexibility, strength, rigidity, enabling it withstand loading without fracturing. Moreover, dentin’s unique architectural features, such as odontoblast processes dentinal tubules spatial compartmentalization odontoblasts dentin sensory neurons contribute distinctive perception external stimuli while acting defensive barrier for dentin-pulp complex. Since architecture governs functions nociception repair response stimuli, understanding mechanobiology developing treatments pain management dentin-associated diseases regeneration. review discusses physical features regulate mechano-sensing, focusing on mechano-sensitive ion channels. Additionally, we explore advanced vitro platforms that mimic providing deeper insights into fundamental mechanobiological phenomena laying groundwork effective mechano-therapeutic strategies diseases.

Language: Английский

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Piezo1 Promotes Odontogenic Differentiation of Dental Pulp Stem Cells Under Stress Conditions

International Dental Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Beyond hydrodynamics: The role of ion channels in dentine hypersensitivity

Journal of Dentistry, Journal Year: 2025, Volume and Issue: unknown, P. 105745 - 105745

Published: April 1, 2025

Language: Английский

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Piezo1 in Digestive System Function and Dysfunction

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(16), P. 12953 - 12953

Published: Aug. 19, 2023

Piezo1, a non-selective cation channel directly activated by mechanical forces, is widely expressed in the digestive system and participates biological functions physiologically pathologically. In this review, we summarized latest insights on Piezo1’s cellular effect across entire system, discussed role of Piezo1 various aspects including ingestion digestion, material metabolism, enteric nervous intestinal barrier, inflammatory response within system. The goal comprehensive review to provide solid foundation for future research about

Language: Английский

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PIEZO1 Promotes Odontoblast-Mediated Reactionary Dentinogenesis via SEMA3A

Journal of Dental Research, Journal Year: 2024, Volume and Issue: 103(9), P. 889 - 898

Published: June 24, 2024

Located at the interface of dentin-pulp complex, odontoblasts are specialized cells responsible for dentin synthesis and nociceptive signal detection in response to external stimuli. Recent studies have shown that mechanosensitive ion channel PIEZO1 is involved bone formation remodeling through influx calcium ions, it abundantly expressed odontoblasts. However, specific role reactionary dentinogenesis underlying mechanisms remain elusive. In this study, we found intense expression plasma membrane cytoplasm healthy human third molars, mouse mandibular odontoblast-like (hOBLCs). hOBLCs, positively regulated DSPP, DMP1, COL1A1 Ca

Language: Английский

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Expression of Piezo1 in the Trigeminal Neurons and in the Axons That Innervate the Dental Pulp

Frontiers in Cellular Neuroscience, Journal Year: 2022, Volume and Issue: 16

Published: June 29, 2022

Information on the neurons and axons that express mechanosensitive channel Piezo1 its expression in innervating dental pulp may help understand nature of Piezo1-mediated mechanosensation underlying mechanism dentin sensitivity elicited by mechanical stimuli. For this, we here investigated neurochemical properties rat trigeminal ganglion (TG) their sensory root human light electron microscopic immunohistochemistry quantitative analysis. was expressed mainly medium-sized large TG neurons. Piezo1-immunopositive (+) frequently coexpressed marker for with myelinated axons, NF200, but rarely markers unmyelinated CGRP or IB4. In TG, primarily small (Aδ, 60.2%) also (Aβ, 24.3%) (C, 15.5%) axons. pulp, numerous NF200+ which formed a network peripheral often “ascended” toward dentin. Most Piezo1+ radicular became where immunoreaction product associated axonal plasma membrane, suggesting functional role pulp. These findings suggest is involved mediating acute pain high-threshold stimuli, mechanotransduction occurs

Language: Английский

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