A four-oscillator model of seasonally adapted morning and evening activities in Drosophila melanogaster DOI Creative Commons
Taishi Yoshii,

Aika Saito,

Tatsuya Yokosako

et al.

Journal of Comparative Physiology A, Journal Year: 2023, Volume and Issue: 210(4), P. 527 - 534

Published: May 23, 2023

Abstract The fruit fly Drosophila melanogaster exhibits two activity peaks, one in the morning and another evening. Because peaks change phase depending on photoperiod they are exposed to, convenient for studying responses of circadian clock to seasonal changes. To explain determination researchers have employed two-oscillator model, which oscillators control peaks. reside different subsets neurons brain, express genes, so-called neurons. However, mechanism underlying is complex requires a new model mechanistic exploration. Here, we hypothesize four-oscillator that controls bimodal rhythms. four regulate evening sleep during midday at night. In this way, rhythms formed by interactions among (two oscillators), may judiciously flexible waveform under conditions. Although still hypothetical, would provide perspective adaptation

Language: Английский

What Drosophila can tell us about state-dependent peptidergic signaling in insects DOI Creative Commons
Dick R. Nässel

Insect Biochemistry and Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 104275 - 104275

Published: Feb. 1, 2025

Language: Английский

Citations

0
A Critical Role for the Fascin Family of Actin Bundling Proteins in Axon Development, Brain Wiring and Function DOI Creative Commons
Katherine R. Hardin,

Arjolyn B. Penas,

Stephan Joubert

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 22, 2025

Abstract Actin-based cell motility drives many neurodevelopmental events including guided axonal growth. Fascin is a major family of F-actin bundling proteins, but its role in axon development and brain wiring unknown. Here, we report that fascin required for development, function. We show enriched the motile filopodia growth cones inhibition impairs extension branching hippocampal neurons culture. next provide evidence essential using Drosophila melanogaster as an vivo model. express single ortholog mammalian called Singed (SN), which highly expressed mushroom body (MB) central nervous system. observe loss SN results drastic MB disruption, highlighted by α- β-lobe defects are consistent with altered guidance. SN-null flies also exhibit defective sensorimotor behaviors assessed negative geotaxis assay. MB-specific expression rescues structure deficits, indicating functions autonomously neurons. Together, our data from primary neuronal culture models highlight critical Significance statement actin-binding proteins (ABPs) crosslink bundle actin filaments to underline membrane protrusions development. Inhibition cultured cells causes it dissipate F-actin-rich become cytosolic, reduces formation, decreases outgrowth branching. In fruit flies, orthologue, Singed, specifically lobes, leads behavioral impairments can be rescued upon Singed.

Language: Английский

Citations

0
On the origin and evolution of the dual oscillator model underlying the photoperiodic clockwork in the suprachiasmatic nucleus DOI
Jennifer A. Evans, William J. Schwartz

Journal of Comparative Physiology A, Journal Year: 2023, Volume and Issue: 210(4), P. 503 - 511

Published: July 23, 2023

Language: Английский

Citations

9
Breaking free from the clock’s tyranny restores memory to brain damaged flies DOI Open Access
Stephane Dissel,

Ellen Morgan,

Lijuan Cao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 26, 2024

Abstract The relationship between sleep and memory is an active topic of investigation. In this context, we demonstrate that enhancing restores to flies with ablated Mushroom Bodies (MB), a key center; consistent across several assays. Mapping the underlying circuitry reveals circadian modulation subset Dopaminergic neurons (DANs) modulate aversive learning. Using imaging, show MB-ablation disrupts, time day these are most responsive. Knocking down receptor for clock output signal, Pigment-dispersing factor (Pdfr), in DANs MB-ablated flies. Crucially, does not result impairments absence functioning clock. Our results reveal neuromodulation’s role cognitive restoration, where aids damaged brains, but unexpectedly hinders process.

Language: Английский

Citations

3
The Never Given 2022 Pittendrigh/Aschoff Lecture: The Clock Network in the Brain—Insights From Insects DOI Creative Commons
Charlotte Helfrich‐Förster

Journal of Biological Rhythms, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

My journey into chronobiology began in 1977 with lectures and internships Wolfgang Engelmann Hans Erkert at the University of Tübingen Germany. At that time, only known animal clock gene was

Language: Английский

Citations

3
Pigment-dispersing factor and CCHamide1 in the Drosophila circadian clock network DOI

Riko Kuwano,

Maki Katsura,

Mai Iwata

et al.

Chronobiology International, Journal Year: 2023, Volume and Issue: 40(3), P. 284 - 299

Published: Feb. 14, 2023

Animals possess a circadian central clock in the brain, where behavioural rhythms are generated. In fruit fly (Drosophila melanogaster), comprises network of approximately 150 neurons, which is important for maintenance coherent and robust rhythm. Several neuropeptides involved have been identified, including Pigment-dispersing factor (PDF) CCHamide1 (CCHa1) neuropeptides. PDF signals bidirectionally to CCHa1-positive neurons; thus, neuron groups expressing CCHa1 interact reciprocally. However, role these interactions molecular remains elusive. this study, we generated Pdf 01 CCHa1SK8 double mutants examined their locomotor activity-related rhythms. The single or displayed free-running under constant dark conditions, whereas 98% were arrhythmic. light-dark evening activity was phase-advanced compared with that mutants. contrast, both had diminished morning activity. These results suggest effects mutation varied parameters. triple per 01, further revealed signalling plays suppression during daytime clock-less background. Our provide insights into between roles

Language: Английский

Citations

7
Spatiotemporal Changes in Netrin/Dscam1 Signaling Dictate Axonal Projection Direction in Drosophila Small Ventral Lateral Clock Neurons DOI Creative Commons
Jingjing Liu, Yuedong Wang, Xian Liu

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: May 14, 2024

Axon projection is a spatial- and temporal-specific process in which the growth cone receives environmental signals guiding axons to their final destination. However, mechanisms underlying changes axonal direction without well-defined landmarks remain elusive. Here, we present evidence showcasing dynamic nature of projections Drosophila ’s small ventral lateral clock neurons (s-LNvs). Our findings reveal that these undergo an initial vertical early larval stage, followed by subsequent transition horizontal early-to-mid third instar larvae. The s-LNv correlates with mushroom body calyx expansion, while s-LNv-expressed Down syndrome cell adhesion molecule (Dscam1) interacts Netrins regulate projection. During specific temporal window, locally newborn dorsal secrete Netrins, facilitating s-LNvs. study establishes compelling vivo model probe switching absence clear landmarks. These underscore significance local microenvironments complementary regulation transitions.

Language: Английский

Citations

2
Molecular and Neural Mechanisms of Temperature Preference Rhythm in Drosophila melanogaster DOI Creative Commons
Tadahiro Goda,

Yujiro Umezaki,

Fumika N. Hamada

et al.

Journal of Biological Rhythms, Journal Year: 2023, Volume and Issue: 38(4), P. 326 - 340

Published: May 24, 2023

Temperature influences animal physiology and behavior. Animals must set an appropriate body temperature to maintain homeostasis maximize survival. Mammals their temperatures using metabolic behavioral strategies. The daily fluctuation in is called the rhythm (BTR). For example, human increases during wakefulness decreases sleep. BTR controlled by circadian clock, closely linked with metabolism sleep, entrains peripheral clocks located liver lungs. However, underlying mechanisms of are largely unclear. In contrast mammals, small ectotherms, such as Drosophila, control choosing environmental temperatures. preferred Drosophila day at night; this pattern referred preference (TPR). As flies close that surrounding environment. Thus, TPR produces BTR, which exhibits a similar BTR. review, we summarize regulatory TPR, including recent studies describe neuronal circuits relaying ambient information dorsal neurons (DNs). neuropeptide diuretic hormone 31 (DH31) its receptor (DH31R) regulate mammalian homolog DH31R, calcitonin (CALCR), also plays important role mouse regulation. addition, both fly separately regulated from another clock output, locomotor activity rhythms. These findings suggest fundamental regulation may be conserved between mammals flies. Furthermore, discuss relationships other physiological functions, dissection could facilitate understanding interaction sleep

Language: Английский

Citations

6
Characterization of clock‐related proteins and neuropeptides in Drosophila littoralis and their putative role in diapause DOI Creative Commons
Giulia Manoli, Meet Zandawala, Taishi Yoshii

et al.

The Journal of Comparative Neurology, Journal Year: 2023, Volume and Issue: 531(15), P. 1525 - 1549

Published: July 26, 2023

Insects from high latitudes spend the winter in a state of overwintering diapause, which is characterized by arrested reproduction, reduced food intake and metabolism, increased life span. The main trigger to enter diapause decreasing day length summer-autumn. It thus assumed that circadian clock acts as an internal sensor for measuring photoperiod orchestrates appropriate seasonal changes physiology metabolism through various neurohormones. However, little known about neuronal organization network neurosecretory system controls high-latitude insects. We addressed this here mapping expression proteins neuropeptides/neurohormones fly Drosophila littoralis. found principal both systems similar melanogaster, but with some striking differences neuropeptide levels patterns. small ventrolateral neurons express pigment-dispersing factor (PDF) short F (sNPF) are most important robust rhythmicity D. melanogaster virtually lack PDF sNPF In contrast, dorsolateral ion transport peptide additionally allatostatin-C appear suited transfer day-length information lateral cells littoralis contain more neuropeptides than melanogaster. Among them, coexpress corazonin, PDF, diuretic hormone 44 control diapause. Our work sets stage investigate roles these diverse regulating insect

Language: Английский

Citations

6
3D‐atlas of the brain of the cockroach Rhyparobia maderae DOI Creative Commons
Vanessa Althaus, Stefanie Jahn, Azar Massah

et al.

The Journal of Comparative Neurology, Journal Year: 2022, Volume and Issue: 530(18), P. 3126 - 3156

Published: Aug. 29, 2022

Abstract The Madeira cockroach Rhyparobia maderae is a nocturnal insect and prominent model organism for the study of circadian rhythms. Its master clock, controlling locomotor activity sleep–wake cycles, located in accessory medulla optic lobe. For better understanding brain regions controlled by clock organization this general, we created three‐dimensional (3D) reconstruction all neuropils cerebral ganglia based on anti‐synapsin anti‐γ‐aminobutyric acid immunolabeling whole mount brains. Forty‐nine major were identified three‐dimensionally reconstructed. Single‐cell dye fills complement data provide evidence distinct subdivisions certain areas. Most defined fruit fly Drosophila melanogaster could be distinguished as well. However, some do not exist entities while others are lacking fly. In addition to neuropils, fiber systems, tracts, commissures reconstructed served important landmarks separating Being insect, R. an new species growing collection 3D atlases only second hemimetabolous which detailed atlas available. This will highly valuable evolutionary comparison greatly facilitate addressing areas that supervised clock.

Language: Английский

Citations

8