Microbiology Research,
Journal Year:
2023,
Volume and Issue:
14(3), P. 1114 - 1123
Published: Aug. 9, 2023
Glaesserella
parasuis
(G.
parasuis)
can
cause
peritonitis
in
piglets.
However,
the
pathogenesis
of
remains
unclear.
Baicalin
has
been
shown
to
possess
anti-inflammatory
and
anti-oxidant
functions.
The
aim
this
study
was
investigate
role
PANX-1/P2X7
axis
P2Y6
signaling
pathway
induced
by
G.
effect
baicain
on
activation
triggered
parasuis.
A
serovar
5
isolate
SH0165
strain
obtained
from
lungs
commercially
produced
pigs
which
had
typical
symptoms
Glässer’s
disease,
namely
arthritis,
fibrinous
polyserositis,
hemorrhagic
pneumonia,
meningitis.
Then,
35
piglets
were
randomly
divided
into
five
groups,
each
group
containing
seven
groups
consisted
a
negative
control
group,
an
infection
25
mg/kg
baicalin
50
100
group.
results
showed
that
could
promote
activation;
induce
NLRP3/caspase-1,
IL-1β
IL-18
expression;
trigger
PLC/PKC
MLCK/MLC
attenuate
expression
tight
junction
proteins
ZO-1,
E-cadherin,
Occludins,
claudin
1;
stimulate
CD14,
CD24,
CD36,
CD47,
CD91
peritoneum
as
measured
via
Western
blot
(p
<
0.01;
PLC,
p
0.05).
significantly
inhibit
axis,
P2Y6,
NLRP3/caspase-1
reduce
occludins,
1
blot.
Our
deepen
understanding
mechanism
provide
some
novel
potential
methods
controlling
infection.
Journal of Dental Research,
Journal Year:
2024,
Volume and Issue:
103(9), P. 889 - 898
Published: June 24, 2024
Located
at
the
interface
of
dentin-pulp
complex,
odontoblasts
are
specialized
cells
responsible
for
dentin
synthesis
and
nociceptive
signal
detection
in
response
to
external
stimuli.
Recent
studies
have
shown
that
mechanosensitive
ion
channel
PIEZO1
is
involved
bone
formation
remodeling
through
influx
calcium
ions,
it
abundantly
expressed
odontoblasts.
However,
specific
role
reactionary
dentinogenesis
underlying
mechanisms
remain
elusive.
In
this
study,
we
found
intense
expression
plasma
membrane
cytoplasm
healthy
human
third
molars,
mouse
mandibular
odontoblast-like
(hOBLCs).
hOBLCs,
positively
regulated
DSPP,
DMP1,
COL1A1
Ca
The Anatomical Record,
Journal Year:
2023,
Volume and Issue:
307(5), P. 1960 - 1968
Published: Nov. 17, 2023
Abstract
PIEZO1
and
PIEZO2
are
essential
components
of
mechanogated
ion
channels,
which
required
for
mechanotransduction
biological
processes
associated
with
mechanical
stimuli.
There
is
evidence
the
presence
in
teeth
periodontal
ligaments,
especially
cell
lines
mice,
but
human
studies
almost
nonexistent.
Decalcified
permanent
mouse
molars
were
processed
immunohistochemical
detection
PIEZO2.
Confocal
laser
microscopy
was
used
to
examine
co‐localization
PIEZO
1
vimentin
(a
marker
differentiated
odontoblasts)
teeth.
In
outer
layer
dental
pulp,
abundant
PIEZO1‐
PIEZO2‐positive
cells
found
that
had
no
odontoblast
morphology
vimentin‐negative.
Based
on
their
morphology,
location,
absence
positivity,
they
identified
as
pulp
stem
or
pre‐odontoblasts.
However,
ubiquitously
detected
colocalized
odontoblasts.
Intense
immunoreactivity
has
been
observed
murine
ligaments.
Our
findings
suggest
may
be
mechanosensors/mechanotransducers
odontoblasts,
well
transmission
forces
by
ligament
humans
mice.
BMC Oral Health,
Journal Year:
2024,
Volume and Issue:
24(1)
Published: April 16, 2024
Abstract
Background
Mechanosensitive
ion
channel
PIEZOs
have
been
widely
reported
to
involve
inflammation
and
pain.
This
study
aimed
clarify
expression
patterns
of
their
potential
relations
irreversible
pulpitis.
Materials
methods
Normal
pulp
tissues
(
n
=
29)
from
patients
with
impacted
third
molars
inflamed
23)
pulpitis
were
collected.
Pain
levels
assessed
using
a
numerical
rating
scale.
PIEZO
expressions
measured
real-time
PCR
then
confirmed
GEO
datasets
GSE77459,
immunoblot,
immunohistochemistry
staining.
Correlations
mRNA
inflammatory
markers,
pain
or
clinical
evaluated
Spearman’s
correlation
analysis.
Univariate
analysis
was
conducted
analyze
based
on
description
examinations
cold
test,
percussion,
palpation,
bite
test.
Results
Compared
normal
tissues,
PIEZO1
significantly
increased
in
while
PIEZO2
decreased,
which
further
GSE77459
protein
histological
level.
The
positive
the
between
as
well
verified.
also
positively
correlated
levels.
Besides,
who
continuous
detected
response
stimulus
exhibited
higher
level
tissues.
By
contrast,
reporting
duration
more
than
one
week
showed
PIEZO1.
Conclusions
demonstrated
upregulation
downregulation
revealed
relation
These
findings
suggested
that
might
play
critical
roles
progression
paved
way
for
investigations
at
novel
therapies
by
targeting
PIEZOs.
European Journal of Pharmacology,
Journal Year:
2024,
Volume and Issue:
984, P. 177052 - 177052
Published: Oct. 10, 2024
P2X3
and
P2X2/3
receptors
are
promising
therapeutic
targets
for
pain
treatment
selective
inhibitors
under
evaluation
in
ongoing
clinical
trials.
Here
we
aim
to
consolidate
quantitatively
evaluate
the
preclinical
evidence
on
treatment.
Biomolecules,
Journal Year:
2023,
Volume and Issue:
13(6), P. 879 - 879
Published: May 23, 2023
Odontoblasts
are
involved
in
sensory
generation
as
receptor
cells
and
dentin
formation.
We
previously
reported
that
an
increase
intracellular
cAMP
levels
by
cannabinoid
1
activation
induces
Ca2+
influx
via
transient
potential
vanilloid
subfamily
member
channels
odontoblasts,
indicating
cAMP/Ca2+
signal
coupling
is
dentinal
pain
reactionary
Here,
dynamics
cultured
human
odontoblasts
were
investigated
to
understand
the
detailed
expression
patterns
of
signaling
pathway
activated
Gs
protein-coupled
clarify
its
role
cellular
functions.
The
presence
plasma
membrane
Gαs
well
prostaglandin
I2
(IP),
5-hydroxytryptamine
5-HT4
(5-HT4),
dopamine
D1
(D1),
adenosine
A2A
(A2A),
vasoactive
intestinal
polypeptide
(VIP)
immunoreactivity
was
observed
odontoblasts.
In
extracellular
Ca2+,
application
agonists
for
IP
(beraprost),
(BIMU8),
(SKF83959),
(PSB0777),
VIP
receptors
increased
levels.
This
inhibited
adenylyl
cyclase
(AC)
inhibitor
SQ22536
each
antagonist,
dose-dependently.
These
results
suggested
express
IP,
5-HT4,
D1,
A2A,
receptors.
addition,
these
activating
AC
Pflügers Archiv - European Journal of Physiology,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 12, 2024
Abstract
In
odontoblasts,
intracellular
Ca
2+
signaling
plays
key
roles
in
reactionary
dentin
formation
and
generation
of
dentinal
pain.
Odontoblasts
also
express
several
G
s
protein-coupled
receptors
that
promote
production
cyclic
AMP
(cAMP).
However,
the
crosstalk
between
cAMP
signaling,
as
well
role
cellular
functions
remains
unclear.
this
study,
we
measured
levels
free
concentration
([Ca
]
i
).
We
investigated
effect
on
mineralization
by
odontoblasts.
presence
extracellular
,
application
forskolin
(adenylyl
cyclase
activator)
or
isoproterenol
(G
beta-2
adrenergic
receptor
agonist)
increased
[Ca
The
increases
could
not
be
observed
removing
indicating
is
capable
to
activate
entry.
Forskolin-induced
increase
was
inhibited
a
protein
kinase
A
inhibitor
sensitive
Gd
3+
2APB,
Zn
but
verapamil,
ML218,
La
.
immunofluorescence
analyses,
odontoblasts
were
immunopositive
for
calcium
homeostasis
modulator
1
(CALHM1),
which
found
close
ionotropic
ATP
subtype,
P2X
3
receptors.
When
CALHM1
knocked
down,
forskolin-induced
suppressed.
Alizarin
red
von
Kossa
staining
showed
decreased
mineralization.
These
findings
suggest
activation
adenylyl
elicited
level
influx
via
Subsequent
cAMP-dependent
mediated
addition,
pathway
negatively
dentinogenesis.
