Zinc Oxide Nanoparticles as Potential Delivery Carrier: Green Synthesis by Aspergillus niger Endophytic Fungus, Characterization, and In Vitro/In Vivo Antibacterial Activity

Pharmaceuticals, Journal Year: 2022, Volume and Issue: 15(9), P. 1057 - 1057

Published: Aug. 26, 2022

We aimed to synthesize zinc oxide nanoparticles (ZnO NPs) using the endophytic fungal extract of Aspergillus niger. The prepared ZnO NPs were characterized, and their in vitro vivo antibacterial activity was investigated. Isolated fungus identification carried out 18S rRNA. A. niger employed for green synthesis NPs. elucidated against Staphylococcus aureus broth microdilution method quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, a systemic infection model mice. biosynthesized showed maximum optical density at 380 nm with characteristic peaks on Fourier-transform infrared spectrum. X-ray diffraction pattern highly matched standard platform crystals. Energy-dispersive analysis confirmed that main composition oxygen atoms. Scanning transmission electron microscopies spherical geometry smooth surface. Zeta potential measurements (26.6 ± 0.56 mV) verified adequate stability Minimum inhibitory concentrations S. isolates ranged from 8 128 µg/mL. revealed antibiofilm activity, resulting downregulation tested biofilm genes 29.17% isolates. Regarding experiment, reduced congestion fibrosis liver spleen tissues. They also improved function, increased survival rate, significantly decreased inflammatory markers (p < 0.05). synthesized by promising action

Language: Английский

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Next-generation probiotics: the upcoming biotherapeutics

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: April 15, 2024

Abstract Recent and continuing advances in gut microbiome research have pointed out the role of microbiota as an unexplored source potentially beneficial probiotic microbes. Along lines these advances, both public awareness acceptance probiotics are increasing. That’s why; academic industrial is dedicated to identifying investigating new microbial strains for development next-generation (NGPs). At this time, there a growing interest NGPs biotherapeutics that alter affect various diseases development. In work, we focused on some emergent promising NGPs, specifically Eubacterium hallii, Faecalibacterium prausnitzii, Roseburia spp., Akkermansia muciniphila , Bacteroides fragilis their presence can impact diseases. Emerging studies point roles open up novel therapeutic options. Interestingly, were found enhance gastrointestinal immunity, immunotherapy efficacy cancer patients, retain intestinal barrier integrity, generate valuable metabolites, especially short-chain fatty acids, decrease complications chemotherapy radiotherapy. Although many considered prevention treatment several chronic diseases, humans still lacking. Therefore, approval microbes from regulatory agencies rare. Besides, issues limit wide use market, such suitable methods culture storage oxygen-sensitive The present review goes over main points related gives viewpoint key hinder application. Furthermore, advancement human healthiness investigations by clarifying limitations traditional microorganisms, discussing characteristics emerging defining management certain ailments. Future should emphasize isolation, mechanisms action probiotics, safety, clinical humans.

Language: Английский

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In Vivo and In Vitro Antimicrobial Activity of Biogenic Silver Nanoparticles against Staphylococcus aureus Clinical Isolates

Pharmaceuticals, Journal Year: 2022, Volume and Issue: 15(2), P. 194 - 194

Published: Feb. 3, 2022

can cause a wide range of severe infections owing to its multiple virulence factors in addition resistance antimicrobials; therefore, novel antimicrobials are needed. Herein, we used

Language: Английский

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Anti-Biofilm and Antibacterial Activities of Cycas media R. Br Secondary Metabolites: In Silico, In Vitro, and In Vivo Approaches

Antibiotics, Journal Year: 2022, Volume and Issue: 11(8), P. 993 - 993

Published: July 24, 2022

Enterococcus species possess many virulence factors that have an essential role in exacerbating the infections caused by them. The current study aimed to evaluate effect of secondary metabolites ginkgetin (GINK) and sotetsuflavone (SOTE), isolated from Cycas media R. Br dichloromethane fraction, on faecalis (E. faecalis) isolates for first time. antibacterial antivirulence activities compounds were investigated using docking studies vitro determination minimum inhibitory concentrations (MICs). Additionally, flow cytometry scanning electron microscope (SEM) utilized assess SOTE tested bacteria. Moreover, crystal violet assay qRT-PCR used test biofilm-forming ability E. isolates. In addition, a systemic infection model was vivo investigate activity SOTE. We found both GINK showed good affinity five proteins enrolled faecalis, with being highest, suggesting possible mechanisms ligands. exhibited higher than GINK, as values MICs lower those GINK. Thus, we performed assays However, they did not exhibit any significant variations (p > 0.05) membrane depolarization evaluated SEM, distortion deformation treated cells. Regarding its impact biofilm formation, it inhibited isolates, determined qRT-PCR. experiment revealed resulted reduction inflammation liver spleen increase survival rate. also improved liver-function tests decreased tumor necrosis factor-alpha immunostaining markers, interleukins (IL-1β IL-6), ELISA. can conclude could be promising compound should future preclinical clinical studies.

Language: Английский

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Bilosomes as Nanoplatform for Oral Delivery and Modulated In Vivo Antimicrobial Activity of Lycopene

Pharmaceuticals, Journal Year: 2022, Volume and Issue: 15(9), P. 1043 - 1043

Published: Aug. 24, 2022

Owing to the disseminating resistance among pathogenic bacteria, especially Klebsiella pneumoniae, there is a high need for alternate compounds with antibacterial activity. Herein, lycopene was isolated from Lycopersicon esculentum L. Molecular docking approach employed explore binding affinity selected vital proteins of K. pneumoniae mechanisms being investigated. This proposed promising activity lycopene. However, pharmacological use hampered by its poor solubility and limited oral bioavailability. Accordingly, bilosomes were fabricated delivery. The computed entrapment efficiency, mean vesicular size, zeta potential values optimized formulation 93.2 ± 0.6%, 485.8 35.3 nm, -38.3 4, respectively. In vitro drug release studies revealed controlled constructed bilosomes, liberation based on Higuchi kinetics model. Transmission electron microscopic evaluation spherical nanovesicles free aggregates. Moreover, in vivo formulations against multidrug-resistant isolates explored. exhibited lowest minimum inhibitory concentrations ranging 8 32 µg/mL. addition, scanning microscopy remarkable deformation lysis bilosomes-treated bacterial cells. Regarding investigation, lung infection model mice employed. tested reduced inflammation congestion treated mice's tissues, resulting normal-sized bronchioles alveoli very few congested vessels. it resulted significant reduction pulmonary fibrosis. conclusion, this study investigated naturally controlling infections triggered isolates. Furthermore, introduced as biocompatible nanocarrier modulation delivery antimicrobial

Language: Английский

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The wound healing effect of botanicals and pure natural substances used in in vivo models

Inflammopharmacology, Journal Year: 2023, Volume and Issue: 31(2), P. 755 - 772

Published: Feb. 22, 2023

Repairing the wound is a multistep process that includes spatial and temporal synchronization of different range cell types to increase speed contraction, proliferation epithelial cells, collagen formation. The need for proper management acute wounds be cured not turned into chronic significant clinical challenge. traditional practice medicinal plants in many regions world has been used healing since ancient times. Recent scientific research introduced evidence efficacy plants, their phyto-components, mechanisms underlying wound-repairing activity. This review aims briefly highlight wound-curing effect plant extracts purely natural substances excision, incision, burn experimental animal models with or without infection mice, rats (diabetic nondiabetic), rabbits last 5 years. vivo studies represented reliable how powerful products are properly. They have good scavenging activity against Reactive oxygen species (ROS) anti-inflammatory antimicrobial effects help healing. It evident incorporating bioactive dressings bio- synthetic polymers nanofiber, hydrogel, film, scaffold, sponge forms showed promising results phases haemostasis, inflammation, growth, re-epithelialization, remodelling.

Language: Английский

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Rosuvastatin and diosmetin inhibited the HSP70/TLR4 /NF-κB p65/NLRP3 signaling pathways and switched macrophage to M2 phenotype in a rat model of acute kidney injury induced by cisplatin

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 171, P. 116151 - 116151

Published: Jan. 22, 2024

Numerous efforts to manage acute kidney injury (AKI) were unsuccessful because its pathophysiology is still poorly understood. Thus, our research hotspot was explore the possible renoprotective effects of rosuvastatin (Ros) and diosmetin (D) on macrophage polarization role HSP70/TLR4/MyD88/NF-κB p65/NLRP3/STAT3 signaling in cis-induced AKI study activity D against uropathogenic bacteria. Fifty-four albino male rats randomized into 9 groups equally: Control, Ros, D20, D40, untreated Cis, Cis cotreated with D40 Ros+D40 for 10 days. Our results indicated that Ros D, a dose-dependent manner, markedly restored body weight, systolic blood pressure, renal histological architecture besides significantly upregulated SOD levels, expression anti-inflammatory CD163 macrophages, arginase1levels, IL-10 levels,STAT3 PCNA immunoreactivity. Also, they downregulated index, serum urea, creatinine, cystatin c, inflammatory biomarkers (C reactive protein, IL1β & TNF-α), MDA p65/NLRP3 expressions, proinflammatory CD68 macrophages caspase-3 immunoreactivity, resulting reversal damage. These findings further confirmed by molecular docking showed binding affinity towards TLR4 NLRP3. Furthermore, had antibacterial action minimum inhibitory concentration ranging from 128 256 µg/mL caused delay growth tested isolates, negatively affected membrane integrity. In conclusion, antioxidant, antiapoptotic properties switched CD163. Additionally, targeting signals are effective therapeutic strategy AKI.

Language: Английский

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Wound-Healing Potential of Rhoifolin-Rich Fraction Isolated from Sanguisorba officinalis Roots Supported by Enhancing Re-Epithelization, Angiogenesis, Anti-Inflammatory, and Antimicrobial Effects

Pharmaceuticals, Journal Year: 2022, Volume and Issue: 15(2), P. 178 - 178

Published: Jan. 31, 2022

A wound is a complicated bioprocess resulting in significant tissue damage, which worsened by secondary bacterial infection, commonly

Language: Английский

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Silver Nanoparticles Prepared Using Encephalartos laurentianus De Wild Leaf Extract Have Inhibitory Activity against Candida albicans Clinical Isolates

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(10), P. 1005 - 1005

Published: Sept. 25, 2022

Candida albicans is a major human opportunistic pathogen causing infections, which range from cutaneous to invasive systemic infections. Herein, the antifungal and anti-biofilm potential of silver nanoparticles (AgNPs) green synthesized in presence Encephalartos laurentianus leaf extract (ELLE) were investigated. The bioactive chemicals ELLE, including phenolics, flavonoids, glycosides identified quantified for first time. AgNPs showed minimum inhibitory concentration (MIC) values against C. clinical isolates ranging 8 256 µg/mL. In addition, significantly decreased biofilm formation. impact on expression genes encoding formation was assessed using qRT-PCR. had beneficial role macroscopic wound healing, they resulted complete epithelization without any granulation tissue or inflammation. Treatment with negative immunostaining tumor necrosis factor-α. levels inflammation markers, interleukin-6 interleukin-1β, (p < 0.05) AgNPs-treated group. There also pronounced increase gene fibronectin platelet-derived growth factor tissues. Thus, ELLE may be promising healing agent.

Language: Английский

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The role of berberine in Covid-19: potential adjunct therapy

Inflammopharmacology, Journal Year: 2022, Volume and Issue: 30(6), P. 2003 - 2016

Published: Oct. 2, 2022

Language: Английский

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