Artificial Cells Nanomedicine and Biotechnology, Journal Year: 2024, Volume and Issue: 52(1), P. 261 - 269
Published: May 2, 2024
The widespread dissemination of bacterial resistance has led to great attention being paid finding substitutes for traditionally used antibiotics. Plants are rich in various phytochemicals that could be as antibacterial therapies. Here, we elucidate the phytochemical profile
Language: Английский
Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)
Published: Jan. 5, 2025
Abstract Carbapenem-resistant Klebsiella pneumoniae poses a severe risk to global public health, necessitating the immediate development of novel therapeutic strategies. The current study aimed investigate effectiveness green algae Arthrospira maxima (commercially known as Spirulina ) both in vitro and vivo against carbapenem-resistant K. . In this study, thirty isolates were collected, identified, then screened for their susceptibility several antibiotics carbapenemase production genes using PCR. Both bla KPC OXA-48 most predominant detected tested isolates. phytochemical profiling A. algal extract was conducted LC–MS/MS positive mode technique. minimum inhibitory concentrations (MIC) ranged from 500 1000 µg/mL. also resulted decreasing membrane integrity distortion bacterial cells revealed by scanning electron microscope. bioactive compounds that responsible antibacterial action fatty acids, including PUFAs, polysaccharides, glycosides, peptides, flavonoids, phycocyanin, minerals, essential amino vitamins. Moreover, an antibiofilm activity crystal violet assay qRT-PCR. A murine pneumonia model employed assessment extract. showed promising which comparable colistin (standard drug). This manifested improving pulmonary architecture, inflammatory cell infiltration, fibrosis after staining with hematoxylin eosin Masson’s trichrome stain. Using immunohistochemical investigations, percentage immunoreactive significantly decreased monoclonal antibodies tumor necrosis factor-alpha interleukin six. So, may be considered new candidate medications. Graphical
Language: Английский
Citations
2
International Immunopharmacology, Journal Year: 2023, Volume and Issue: 120, P. 110335 - 110335
Published: May 17, 2023
Language: Английский
Citations
38
Inflammopharmacology, Journal Year: 2023, Volume and Issue: 31(2), P. 755 - 772
Published: Feb. 22, 2023
Repairing the wound is a multistep process that includes spatial and temporal synchronization of different range cell types to increase speed contraction, proliferation epithelial cells, collagen formation. The need for proper management acute wounds be cured not turned into chronic significant clinical challenge. traditional practice medicinal plants in many regions world has been used healing since ancient times. Recent scientific research introduced evidence efficacy plants, their phyto-components, mechanisms underlying wound-repairing activity. This review aims briefly highlight wound-curing effect plant extracts purely natural substances excision, incision, burn experimental animal models with or without infection mice, rats (diabetic nondiabetic), rabbits last 5 years. vivo studies represented reliable how powerful products are properly. They have good scavenging activity against Reactive oxygen species (ROS) anti-inflammatory antimicrobial effects help healing. It evident incorporating bioactive dressings bio- synthetic polymers nanofiber, hydrogel, film, scaffold, sponge forms showed promising results phases haemostasis, inflammation, growth, re-epithelialization, remodelling.
Language: Английский
Citations
35
Frontiers in Molecular Biosciences, Journal Year: 2023, Volume and Issue: 9
Published: Jan. 11, 2023
The occurrence of chronic wounds is a major global health issue. These are difficult to heal as result disordered healing mechanisms. most common types diabetic ulcers, pressure arterial/venous ulcers and nonhealing surgical wounds. Although bacteria an important cause wounds, fungi also play substantial role in them. fungal infection rate varies with different wound types, but overall, the prevalence extremely underestimated clinical treatment management Wounds can be colonized by host cutaneous, commensal or environmental evolve into local infections, causing fungemia well invasive disease. Furthermore, involved wound-related infections help resist antibiotics immune response, forcing become reservoirs for multiresistant species, which considered potential key factor microbial bioburden ulcers. Fungi recalcitrant process. Biofilm establishment predominant mechanism resistance tolerance antimicrobials Candida albicans yeast Trichophyton rubrum filamentous main infection. Fungal species diversity drug phenotypes will emphasized. In this review, we outline latest research on discuss challenges future perspectives related diagnosing managing
Language: Английский
Citations
34
Heliyon, Journal Year: 2023, Volume and Issue: 9(6), P. e16853 - e16853
Published: June 1, 2023
This study showed the anti-candida, biofilm inhibitory, antioxidant, anticoagulant, and thrombolytic properties of biogenic silver nanoparticles (AgNPs) fabricated by using supernatant Penicillium fimorum (GenBank accession number OQ568180) isolated from soil. The AgNPs were characterized different analytical techniques. A sharp surface plasmon resonance (SPR) peak colloidal at 429.5 nm in UV-vis spectrum confirmed fabrication nanosized particles. broth microdilution assay anti-candida with a minimum inhibitory concentration (MIC) 4 μg mL-1. In next step, protein DNA leakage assays as well reactive oxygen species (ROS) performed to evaluate possible mechanisms representing an increase total along climb-up ROS levels AgNPs-treated samples. Flow cytometry also dose-dependent cell death Further studies performance against Candia albicans. concentrations MIC 4*MIC inhibited 79.68 ± 14.38% 83.57 3.41% formation C. albicans, respectively. Moreover, this that intrinsic pathway may play significant role anticoagulant AgNPs. addition, 500 mL-1, represented 49.27%, 73.96 2.59% DPPH radical scavenging potential, Promising biological suggests these nanomaterials good candidate for biomedical pharmaceutical applications.
Language: Английский
Citations
24
Journal of Nanomaterials, Journal Year: 2024, Volume and Issue: 2024, P. 1 - 48
Published: April 12, 2024
Nanotechnology has emerged as a promising field in pharmaceutical research, involving producing unique nanoscale materials with sizes up to 100 nm via physiochemical and biological approaches. Nowadays more emphasis been given eco-friendly techniques for developing nanomaterials enhance their applications minimize health environmental risks. With the help of green nanotechnology, wide range metal, metal oxide, bimetallic nanoparticles distinct chemical compositions, sizes, morphologies have manufactured which are safe, economical, environment friendly. Due biocompatibility vast potential biomedical (antibacterial, anticancer, antiviral, analgesic, anticoagulant, biofilm inhibitory activity) other fields such (nanofertilizers, fermentative, food, bioethanol production, construction field), garnered significant interest worldwide. The precursors combined natural extracts plants, algae, fungi, bacteria get potent novel Ag, Au, Co, Cu, Fe, Zr, Zn, Ni, Pt, Mg, Ti, Pd, Cd, Bi2O3, CeO2, Co3O4, CoFe2O4, CuO, Fe2O3, MgO, NiO, TiO2, ZnO, ZrO2, Ag-Au, Ag-Cr, Ag-Cu, Ag-Zn, Ag-CeO2, Ag-CuO, Ag-SeO2, Ag-TiO2, Ag-ZnO, Cu-Ag, Cu-Mg, Cu-Ni, Pd-Pt, Pt-Ag, ZnO-CuO, ZnO-SeO, ZnO-Se, Se-Zr, Co-Bi2O3. These plant-mediated possess excellent antibacterial anticancer activity when tested against several microorganisms cancer cell lines. Plants contain essential phytoconstituents (polyphenols, flavonoids, terpenoids, glycosides, alkaloids, etc.) compared sources (bacteria, algae) higher concentration that play vital role development because these plant-phytoconstituents act reducing, stabilizing, capping agent helps nanoparticles. After concluding all findings, this review designed first time way it imparts satisfactory knowledge about together, along mechanisms. Additionally, provides information characterization (UV–vis, FT-IR, DLS, XRD, SEM, TEM, BET, AFM) employed nanoparticles, applications, industries. Hence, various types versatile application diverse is not covered pieces literature.
Language: Английский
Citations
13
European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)
Published: Feb. 9, 2024
Abstract Multiple sclerosis (MS) is the most frequent inflammatory and demyelinating disease of central nervous system (CNS). The underlying pathophysiology MS destruction myelin sheath by immune cells. formation plaques, inflammation, injury neuronal characterizes its neuropathology. plaques are multiple focal regions demyelination disseminated in brain's white matter, spinal cords, deep grey cerebral cortex. Fenofibrate a peroxisome proliferative activated receptor alpha (PPAR-α) that attenuates reactions MS. inhibits differentiation Th17 inhibiting expression pro-inflammatory signaling. According to these findings, this review intended illuminate mechanistic immunoinflammatory role fenofibrate mitigating In conclusion, can attenuate neuropathology modulating different pathways, including oxidative stress, autophagy, mitochondrial dysfunction, inflammatory-signaling neuroinflammation.
Language: Английский
Citations
9
Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 165, P. 115010 - 115010
Published: June 19, 2023
Doxorubicin (DOX) is a widely used chemotherapeutic agent for various tumors treatment; apart from its activity, the traditional usage of DOX has been limited by adverse effects on multiple organs, mainly hepatotoxicity. The molecular mechanisms underlying hepatotoxicity are due to production reactive oxygen species (ROS) inducing oxidative stress, diminishing antioxidant enzymes, apoptosis, inflammation, and mitochondrial dysfunction. Thus, there an urgent need develop therapy that minimizes widens use in types cancers without fear serious Ginkgetin (GINK), natural biflavonoid, exhibits diverse actions, including promising free radical scavenging, antioxidant, anti-inflammatory activities. So, this study's objectives were determine whether GINK could mitigate DOX's hepatotoxic look into putative hepatoprotective pathway. Mice divided five groups: Normal control, control 100, Untreated group, groups treated with (50 100 mg/kg) intraperitoneally daily four days before administration additional three afterward. pretreatment showed marked protection also attenuation histopathological structural alterations. These outcomes corroborated biochemically considerable decrease alanine aminotransferases, aspartate aminotransferase, alkaline phosphatase levels. significantly augmented silent information regulator 1 nuclear translocation NF-E2-related factor 2 repressed expression protein levels forkhead box O1, inducible nitric oxide synthase, P53 relative group. alleviated stress induced significant via suppression interleukin-6, Kabba B, iNOS respectively. This study first investigate GINK's potentially beneficial acute hepatotoxicity, possibly exhibiting anti-inflammatory, anti-apoptotic modulation Sirt1/FOXO-1/NF-κB Signal.
Language: Английский
Citations
13
Journal of Zhejiang University SCIENCE B, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 25, 2025
Abstract Ulcerative colitis (UC) is an inflammatory condition of the intestine, resulting from increase in oxidative stress and pro-inflammatory mediators. In this study, extract endophytic bacterium Rhizobium aegyptiacum was prepared for first time using liquid chromatography-mass spectrometry (LC-MS). addition, also time, protective potential R. revealed vivo rat model UC. The animals were grouped into four categories: normal control (group I), II), acetic acid (AA)-induced UC III), -treated AA-induced IV). group IV, administered at 0.2 mg/kg daily one week before two weeks after induction After sacrificing rats on last day experiment, colon tissues collected subjected to histological, immunohistochemical, biochemical investigations. There a remarkable improvement histological findings as by hematoxylin eosin (H&E) staining, Masson’s trichrome periodic acid-Schiff (PAS) staining. Normal mucosal surfaces covered with straight, intact, thin brush border revealed. Goblet cells appeared magenta color, there significant decrease distribution collagen fibers mucosa submucosal connective tissues. All these comparable respective characteristics group. Regarding cyclooxygenase-2 (COX-2) immunostaining, weak immune reaction shown most cells. Moreover, examined scanning electron microscope, which confirmed results assessment. A regular polygonal unit pattern seen crypt orifices different sizes numerous goblet Furthermore, levels catalase (CAT), myeloperoxidase (MPO), nitric oxide (NO), interleukin-6 (IL-6), interlukin-1β (IL-1β) determined colonic groups colorimetric assay enzyme-linked immunosorbent (ELISA). comparison III, IV exhibited rise ( P <0.05) CAT level but substantial decline NO, MPO, cytokine (IL-6 IL-1 β) levels. Based reverse transcription-quantitative polymerase chain (RT-qPCR), tumor necrosis factor-α TNF-α ) gene expression upregulated significantly downregulated treatment IV. On contrary, heme oxygenase-1 HO-1 substantially Our imply that oral consumption ameliorates restoring reestablishing integrity, addition its anti-oxidant anti-inflammatory effects. Accordingly, potentially effective beneficial human therapy, needs be further investigated future work.
Language: Английский
Citations
0
Journal of Pharmaceutical Innovation, Journal Year: 2025, Volume and Issue: 20(2)
Published: March 6, 2025
Language: Английский
Citations
0