IntechOpen eBooks, Journal Year: 2023, Volume and Issue: unknown
Published: Nov. 2, 2023
Language: Английский
Life, Journal Year: 2023, Volume and Issue: 13(1), P. 148 - 148
Published: Jan. 4, 2023
Urinary tract infections (UTIs) represent one of the most common that are frequently encountered in health care facilities. One main mechanisms used by bacteria allows them to survive hostile environments is biofilm formation. Biofilms closed bacterial communities offer protection and safe hiding, allowing evade host defenses hide from reach antibiotics. Inside communities, show an increased rate horizontal gene transfer exchange resistance virulence genes. Additionally, communication within orchestrate expression genes, which further cements infestation increases invasiveness infection. These facts stress necessity continuously updating our information understanding etiology, pathogenesis, eradication methods this growing public concern. This review seeks understand role formation recurrent urinary tact outlining underlying different uropathogens, addition shedding light on some strategies.
Language: Английский
Citations
64
Biology, Journal Year: 2023, Volume and Issue: 12(4), P. 504 - 504
Published: March 26, 2023
Protecting food from bacterial contamination is crucial for ensuring its safety and avoiding foodborne illness. Serratia marcescens one of the contaminants that can form biofilms pigments spoil product could cause infections illness to consumer. Food preservation essential diminish such or at least reduce their pathogenesis; however, it should not affect odor, taste, consistency must be safe. Sodium citrate a well-known safe additive current study aims evaluate anti-virulence anti-biofilm activity low concentrations against S. marcescens. The antibiofilm activities sodium were evaluated phenotypically genotypically. results showed significant effect on decreasing biofilm formation other virulence factors, as motility production prodigiosin, protease, hemolysins. This owed downregulating virulence-encoding genes. An in vivo investigation was conducted mice histopathological examination isolated tissues liver kidney confirmed citrate. In addition, an silico docking binding ability quorum sensing (QS) receptors regulates virulence. marked virtual compete QS proteins, which explain citrate's effect. conclusion, used prevent by bacteria.
Language: Английский
Citations
30
World Journal of Diabetes, Journal Year: 2023, Volume and Issue: 14(6), P. 680 - 704
Published: June 14, 2023
Diabetes is a chronic disease that considered one of the most stubborn global health problems continues to defy efforts scientists and physicians. The prevalence diabetes in population grow alarming levels year after year, causing an increase incidence complications care costs all over world. One major complication high susceptibility infections especially lower limbs due immunocompromised state diabetic patients, which definitive factor cases. Diabetic foot continue be common patients are associated with risk serious such as bone infection, limb amputations, life-threatening systemic infections. In this review, we discussed circumstances infection well some commonly isolated pathogens from related virulence behavior. addition, shed light on different treatment strategies aim at eradicating infection.
Language: Английский
Citations
27
Trends in Microbiology, Journal Year: 2023, Volume and Issue: 31(7), P. 749 - 762
Published: Feb. 26, 2023
Language: Английский
Citations
26
Biomedicines, Journal Year: 2023, Volume and Issue: 11(5), P. 1442 - 1442
Published: May 14, 2023
The repurposing of drugs is one the most competent strategies for discovering new antimicrobial agents. Vildagliptin a dipeptidyl peptidase-4 inhibitor (DPI-4) that used effectively in combination with metformin to control blood glucose levels diabetic patients. This study was designed evaluate anti-virulence activities this against clinically important pathogens, Pseudomonas aeruginosa. current findings show significant ability vildagliptin-metformin diminish biofilm formation, bacterial motility, and production virulent extracellular enzymes pyocyanin pigment. Furthermore, drug significantly increased susceptibility P. aeruginosa oxidative stress, indicating immunity enhancement eradication cells. In compliance vitro findings, histopathological photomicrographs mice showed considerable protective effect metformin-vildagliptin aeruginosa, revealing relief inflammation due aeruginosa-induced pathogenesis. mainly employs quorum sensing (QS) systems its huge arsenal virulence factors. can be interrupted by anti-QS both vildagliptin, as exhibited affinity QS receptors. Additionally, downregulated expression main three QS-encoding genes These at very low concentrations (10, 1.25 mg/mL, respectively) compared (850, 50 diabetes.
Language: Английский
Citations
25
Saudi Pharmaceutical Journal, Journal Year: 2024, Volume and Issue: 32(5), P. 102041 - 102041
Published: March 18, 2024
The rise of antibiotic resistance in bacteria is becoming a global concern, particularly due to the dwindling supply new antibiotics. This situation mandates discovery antimicrobial candidates. Plant-derived natural compounds have historically played crucial role development antibiotics, serving as rich source substances possessing properties. Numerous studies supported reputation 6-gingerol, prominent compound found ginger family, for its antibacterial In this study, activities 6-gingerol were evaluated against Gram-negative bacteria, Acinetobacter baumannii and Klebsiella pneumoniae, with particular focus on clinically significant Pseudomonas aeruginosa Gram-positive Staphylococcus aureus. Furthermore, anti-virulence assessed vitro, vivo, silico. current findings showed that 6-gingerol's activity effect disruption bacterial cell membrane efflux pumps, it significantly decreased disrupted S. aureus P. aeruginosa. biofilm formation production virulence factors concentrations below MICs. properties could be attributed capacity disrupt virulence-regulating systems; quorum sensing (QS). 6-Gingerol was interact QS receptors downregulate genes responsible QS. addition, molecular docking, dynamics (MD) simulation results indicated comparable binding affinity co-crystalized ligands different targets well stable interactions during 100 ns MD simulations. These suggest holds promise an agent can combined antibiotics treatment severe infections.
Language: Английский
Citations
13
Nanotechnology Reviews, Journal Year: 2024, Volume and Issue: 13(1)
Published: Jan. 1, 2024
Abstract Bacterial biofilms contribute to increased pathogenesis and bacterial resistance. Biofilms can enhance pathogenicity by shielding bacteria from the immune system antibiotics, they are associated with persistent infections. Additionally, antibiotic resistance mechanisms within make them challenging treat, emphasizing need for strategies be addressed. Mitigating virulence is a promising strategy that could ease their eradication host immunity without stressing induce The merits of this augmented when using safe anti-virulence candidates in proper formulations. current study aimed evaluate antibiofilm efficacy curcumin–zein nanoparticles against Pseudomonas aeruginosa . In vitro investigations were performed assess effect on biofilm formation, motility, production factors, including proteases, hemolysins, pyocyanin, comparison bulk curcumin. Furthermore, expression genes encode quorum sensing (QS) systems regulate was assessed. An silico done affinity curcumin QS receptors. an vivo protection assay inhibitory our preparation diminishing P. aeruginosa’s capacity pathogenesis. results showed significant activities compared These attributed curcumin’s interfering its virulence. conclusion, acquires anti-QS, anti-virulence, vastly enhanced upon loading zein nanoparticles.
Language: Английский
Citations
11
Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15
Published: March 12, 2024
The development of resistance to carbapenems in Klebsiella pneumoniae due the production metallo-β-lactamases (MBLs) is a critical public health problem because are last-resort drugs used for treating severe infections extended-spectrum β-lactamases (ESBLs) producing K. . Restoring activity by inhibition valuable approach combat carbapenem resistance. In this study, two well-characterized clinical multidrug and carbapenem-resistant isolates were used. sub-inhibitory concentrations pantoprazole well-reported metallo-β-lactamase inhibitor captopril inhibited hydrolytic activities metallo-β-lactamases, with having more inhibiting activities. Both drugs, when combination meropenem, exhibited synergistic Pantoprazole could also downregulate expression genes bla NDM VIM A docking study revealed that bind chelate zinc ions New Delhi Verona integron-encoded MBL (VIM) enzymes higher affinity than control drug comparable natural ligand indicating significant inhibitory against metallo-β-lactamases. conclusion, can be meropenem as new strategy serious caused
Language: Английский
Citations
7
Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14
Published: April 4, 2024
Pseudomonas aeruginosa belongs to the critical pathogens that represent a global public health problem due their high rate of resistance as listed by WHO. P. can result in many nosocomial infections especially individuals with compromised immune systems. Attenuating virulence factors interference quorum sensing (QS) systems is promising approach treat -resistant infections. Thymoquinone natural compound isolated from Nigella sativa (black seed) essential oil. In this study, minimum inhibitory concentration thymoquinone was detected followed investigating antibiofilm and antivirulence activities subinhibitory against PAO1. The effect on expression QS genes assessed quantitative real-time PCR, protective pathogenesis PAO1 mice mouse survival test. significantly inhibited biofilm, pyocyanin, protease activity, swarming motility. At molecular level, markedly downregulated lasI , l asR rhlI rhlR . Moreover, could protect pathologic effects increasing 20% 100%. conclusion, agent be used an adjunct therapeutic antibiotics attenuate pathogenicity
Language: Английский
Citations
7
Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15
Published: May 21, 2024
Background P. aeruginosa , a significant bacterium, can cause severe illness and resistance to antibiotics. Quorum sensing (QS) systems regulate virulence factors production. Targeting QS could reduce bacteria pathogenicity prevent antibiotic resistance. Cruciferous vegetables contain sulforaphane, known for its anti-inflammatory, antioxidant, anticancer, antimicrobial properties. Aim We aimed examine the inhibitory influences of at sub-inhibitory concentration (¼ minimum concentration, MIC), on in . Materials methods The sulforaphane’s anti-virulence actions concentrations were explored vitro vivo A sub-MIC sulforaphane was combined with anti-pseudomonal drugs, results this combination assessed. virtual affinity receptors studied, effect expression genes quantified. Results Sulforaphane significantly decreased biofilm formation, motility, ability withstand oxidative stress, synthesis extracellular enzymes such as proteases, hemolysins, elastase, well other like pyocyanin. In addition, lessened severity infection mice. reduced antipseudomonal antibiotics’ MICs when used together, resulting synergistic effects. observed impacts attributed inhibit via suppressing genes’ expression. Conclusion shows promise potent anti-QS agent that be alongside conventional antimicrobials manage infections effectively. Furthermore, study paves way further investigation similar structures pharmacophores candidates.
Language: Английский
Citations
7