Quorum Sensing Inhibitors: An Alternative Strategy to Win the Battle against Multidrug-Resistant (MDR) Bacteria

Molecules, Journal Year: 2024, Volume and Issue: 29(15), P. 3466 - 3466

Published: July 24, 2024

Antibiotic resistance is a major problem and global health concern. In total, there are 16 million deaths yearly from infectious diseases, at least 65% of diseases caused by microbial communities that proliferate through the formation biofilms. overuse has resulted in evolution multidrug-resistant (MDR) strains. As result, now much more interest non-antibiotic therapies for bacterial infections. Among these revolutionary, non-traditional medications quorum sensing inhibitors (QSIs). Bacterial cell-to-cell communication known as (QS), it mediated tiny diffusible signaling molecules autoinducers (AIs). QS dependent on density population. used Gram-negative Gram-positive bacteria to control wide range processes; both scenarios, entails synthesis, identification, reaction chemicals, also auto-inducers. Since usual processes regulated expression virulence factors creation biofilms, being investigated an alternative solution antibiotic resistance. Consequently, use QS-inhibiting agents, such QSIs quenching (QQ) enzymes, interfere with seems like good strategy prevent This review sheds light inhibition mechanisms discusses how using this approach can aid winning battle against resistant bacteria.

Language: Английский

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Utilization of zein nano-based system for promoting antibiofilm and anti-virulence activities of curcumin against Pseudomonas aeruginosa

Nanotechnology Reviews, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 1, 2024

Abstract Bacterial biofilms contribute to increased pathogenesis and bacterial resistance. Biofilms can enhance pathogenicity by shielding bacteria from the immune system antibiotics, they are associated with persistent infections. Additionally, antibiotic resistance mechanisms within make them challenging treat, emphasizing need for strategies be addressed. Mitigating virulence is a promising strategy that could ease their eradication host immunity without stressing induce The merits of this augmented when using safe anti-virulence candidates in proper formulations. current study aimed evaluate antibiofilm efficacy curcumin–zein nanoparticles against Pseudomonas aeruginosa . In vitro investigations were performed assess effect on biofilm formation, motility, production factors, including proteases, hemolysins, pyocyanin, comparison bulk curcumin. Furthermore, expression genes encode quorum sensing (QS) systems regulate was assessed. An silico done affinity curcumin QS receptors. an vivo protection assay inhibitory our preparation diminishing P. aeruginosa’s capacity pathogenesis. results showed significant activities compared These attributed curcumin’s interfering its virulence. conclusion, acquires anti-QS, anti-virulence, vastly enhanced upon loading zein nanoparticles.

Language: Английский

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Unveiling the hidden language of bacteria: anti-quorum sensing strategies for gram-negative bacteria infection control

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(3)

Published: Feb. 27, 2024

Language: Английский

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Phenazine Scaffolds as a Potential Allosteric Inhibitor of LasR Protein in Pseudomonas aeruginosa

Bioinformatics and Biology Insights, Journal Year: 2025, Volume and Issue: 19

Published: Jan. 1, 2025

Millions of individuals suffer from chronic infections caused by bacterial biofilms, resulting in significant loss life. Pseudomonas aeruginosa stands out as a major culprit causing such infections, largely due to its antibiotic resistance. This pathogen poses considerable threat healthcare settings, particularly critically ill and immunocompromised patients. The persistence recurrent is often attributed biofilms. Therefore, there an urgent need discover novel small molecules capable efficiently eliminating biofilms independent growth. In this project, silico drug discovery approach was employed identify nine halogenated-phenazine compounds allosteric inhibitors the LasR protein. key transcription factor that triggers other quorum-sensing systems plays crucial role biofilm formation activation virulence genes. By inhibiting LasR, specifically targeting site, dimerization subsequent could be prevented. Molecular docking simulations, coupled with binding energy calculations, identified five potential anti-biofilm agents. These exhibited higher affinities distal suggesting their structural capability interact site residues Based on these findings, it proposed serve promising leads for treatment quorum-sensing-related infections.

Language: Английский

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Antimicrobial and anti-virulence activities of 4-shogaol from grains of paradise against gram-negative bacteria: Integration of experimental and computational methods

Journal of Ethnopharmacology, Journal Year: 2023, Volume and Issue: 323, P. 117611 - 117611

Published: Dec. 27, 2023

Language: Английский

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Thymoquinone is a natural antibiofilm and pathogenicity attenuating agent in Pseudomonas aeruginosa

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: April 4, 2024

Pseudomonas aeruginosa belongs to the critical pathogens that represent a global public health problem due their high rate of resistance as listed by WHO. P. can result in many nosocomial infections especially individuals with compromised immune systems. Attenuating virulence factors interference quorum sensing (QS) systems is promising approach treat -resistant infections. Thymoquinone natural compound isolated from Nigella sativa (black seed) essential oil. In this study, minimum inhibitory concentration thymoquinone was detected followed investigating antibiofilm and antivirulence activities subinhibitory against PAO1. The effect on expression QS genes assessed quantitative real-time PCR, protective pathogenesis PAO1 mice mouse survival test. significantly inhibited biofilm, pyocyanin, protease activity, swarming motility. At molecular level, markedly downregulated lasI , l asR rhlI rhlR . Moreover, could protect pathologic effects increasing 20% 100%. conclusion, agent be used an adjunct therapeutic antibiotics attenuate pathogenicity

Language: Английский

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Investigating Sulforaphane’s anti-virulence and anti-quorum sensing properties against Pseudomonas aeruginosa

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: May 21, 2024

Background P. aeruginosa , a significant bacterium, can cause severe illness and resistance to antibiotics. Quorum sensing (QS) systems regulate virulence factors production. Targeting QS could reduce bacteria pathogenicity prevent antibiotic resistance. Cruciferous vegetables contain sulforaphane, known for its anti-inflammatory, antioxidant, anticancer, antimicrobial properties. Aim We aimed examine the inhibitory influences of at sub-inhibitory concentration (¼ minimum concentration, MIC), on in . Materials methods The sulforaphane’s anti-virulence actions concentrations were explored vitro vivo A sub-MIC sulforaphane was combined with anti-pseudomonal drugs, results this combination assessed. virtual affinity receptors studied, effect expression genes quantified. Results Sulforaphane significantly decreased biofilm formation, motility, ability withstand oxidative stress, synthesis extracellular enzymes such as proteases, hemolysins, elastase, well other like pyocyanin. In addition, lessened severity infection mice. reduced antipseudomonal antibiotics’ MICs when used together, resulting synergistic effects. observed impacts attributed inhibit via suppressing genes’ expression. Conclusion shows promise potent anti-QS agent that be alongside conventional antimicrobials manage infections effectively. Furthermore, study paves way further investigation similar structures pharmacophores candidates.

Language: Английский

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Relocating Glyceryl Trinitrate as an Anti-Virulence Agent against Pseudomonas aeruginosa and Serratia marcescens: Insights from Molecular and In Vivo Investigations

Microorganisms, Journal Year: 2023, Volume and Issue: 11(10), P. 2420 - 2420

Published: Sept. 28, 2023

The problem of antibiotic resistance is a global critical public health concern. In light the threat returning to pre-antibiotic era, new alternative approaches are required such as quorum-sensing (QS) disruption and virulence inhibition, both which apply no discernible selective pressure on bacteria, therefore mitigating potential for development resistant strains. Bearing in mind significant role QS orchestrating bacterial virulence, disrupting becomes essential effectively diminishing virulence. This study aimed assess use sub-inhibitory concentration (0.25 mg/mL) glyceryl trinitrate (GTN) inhibit Serratia marcescens Pseudomonas aeruginosa. GTN could decrease expression genes tested bacteria manner. Histopathological revealed ability alleviate congestion hepatic renal tissues infected mice reduce leukocyte infiltration. recommends topical treat infection caused by P. aeruginosa S. combination with antibiotics.

Language: Английский

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Vitamin D3 potentiates antimicrobial and antibiofilm activities of streptomycin and thymoquinone against Pseudomonas aeruginosa

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(3)

Published: March 1, 2025

Language: Английский

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Arctigenin from Burdock Root Exhibits Potent Antibacterial and Anti-Virulence Properties against Pseudomonas aeruginosa

Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 34(8), P. 1642 - 1652

Published: June 17, 2024

Language: Английский

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