Dietary Antioxidants in the Treatment of Male Infertility: Counteracting Oxidative Stress

Biology, Journal Year: 2021, Volume and Issue: 10(3), P. 241 - 241

Published: March 20, 2021

Infertility affects about 15% of the population and male factors only are responsible for ~25–30% cases infertility. Currently, etiology suboptimal semen quality is poorly understood, many environmental genetic factors, including oxidative stress, have been implicated. Oxidative stress an imbalance between production free radicals, or reactive oxygen species (ROS), capacity body to counteract their harmful effects through neutralization by antioxidants. The purpose this review, employing joint expertise international researchers specialized in nutrition fertility areas, update knowledge reproductive consequences excessive ROS concentrations on Assisted Reproduction Techniques (ART) clinical outcomes, discuss role antioxidants finally why foods dietary patterns more innocuous long term solution ameliorating therefore results ART outcomes. Since a narrative review not systematic/meta-analysis, summarized information present study should be considered cautiously.

Language: Английский

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Whole-exome sequencing of a cohort of infertile men reveals novel causative genes in teratozoospermia that are chiefly related to sperm head defects

Human Reproduction, Journal Year: 2021, Volume and Issue: 37(1), P. 152 - 177

Published: Oct. 13, 2021

Abstract STUDY QUESTION Can whole-exome sequencing (WES) and in vitro validation studies identify new causative genes associated with teratozoospermia, particularly for sperm head defect? SUMMARY ANSWER We investigated a core group of infertile patients, including 82 cases unexplained abnormal 67 individuals multiple morphological abnormalities the flagella (MMAF), revealed rare novel deleterious gene variants correlated or tail defects. WHAT IS KNOWN ALREADY Teratozoospermia is one most common factors causing male infertility. Owing to high phenotypic variability, currently known genetic causes teratozoospermia can only explain rather minor component patients anomalous sperm-head shapes, agents responsible atypical shapes remain largely unknown. DESIGN, SIZE, DURATION executed WES analysis Chinese cohort (N = 149) defective head. also sought reveal influence different morphology on ICSI outcome. PARTICIPANTS/MATERIALS, SETTING, METHODS In this study, 149 men (82 MMAF) were recruited. implemented analyzed negative effects mutations candidate their protein conformations and/or expression. genes’ spatiotemporal expression/localization during spermatogenesis both humans mice, explored interactions proteins that are be involved development. compared outcomes affected various aberrations morphology. MAIN RESULTS AND THE ROLE OF CHANCE identified piwi like RNA-mediated silencing 4 (PIWIL4: 1/82 1.21%), coiled-coil C2 domain containing 1B (CC2D1B: cyclin B3 (CCNB3: KIAA1210 (KIAA1210: 2/82 2.43%) choline phosphotransferase 1 (CHPT1: which correlates head; functional evidence supports roles all these formation. The septin 12 (SEPTIN12: suggested acrosome additionally observed dynein axonemal heavy chain 2 (DNAH2: 1/67 1.49%), 10 (DNAH10: 1.49%) (DNAH12: MMAF, significantly lower fertilization rate MMAF following ICSI. Consequently, our study suggests PIWIL4 CC2D1B might circumvented by degree, CHPT1 loss-of-function failed treatment. LIMITATIONS, REASONS FOR CAUTION we discovered relationship between genotype phenotype deformities humans. However, molecular mechanism relevant development needs further illuminated future research. Furthermore, should provided using knockout/knock-in mouse models additional confirmation spermatogenesis. WIDER IMPLICATIONS FINDINGS This documents For first time, suggest SEPTIN12 related human acrosomal hypoplasia, CCNB3 globozoospermia uncovered two genes—KIAA1210 CHPT1associated biogenesis small absent acrosomes. Additionally, it postulated have contribution demonstrate morphologies indicates may play significant role failure. Our findings therefore provide valuable information diagnosis respect will allow clinicians adopt optimal treatment strategy develop personalized medicine directly targeting effects. FUNDING/COMPETING INTEREST(S) work was financed West China Second University Hospital Sichuan (KS369 KL042). authors declare they do not any conflicts interests. TRIAL REGISTRATION NUMBER N/A.

Language: Английский

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Resveratrol Improves the Frozen-Thawed Ram Sperm Quality

Animals, Journal Year: 2023, Volume and Issue: 13(24), P. 3887 - 3887

Published: Dec. 18, 2023

Cryopreservation generates a substantial quantity of ROS in semen, leading to decline sperm quality and fertilization capacity. The objective this study was investigate the effects resveratrol its optimal concentration on ram after cryopreservation. Ram semen diluted with freezing medium containing different concentrations (0, 25, 50, 75, 100 μM). After thawing, various parameters such as total motility, progressive acrosome integrity, plasma membrane mitochondrial potential, glutathione (GSH) content, synthase (GPx) activity, superoxide dismutase (SOD) catalase (CAT) lipid peroxidation (LPO) malondialdehyde (MDA) level, SIRT1 DNA oxidative damage, AMPK phosphorylation level were assessed. In addition, post-thaw apoptosis evaluated. Comparatively, addition up 75 μM significantly improved motility cryopreserved sperm. Specifically, 50 demonstrated notable enhancement antioxidant capacity, adenosine triphosphate (ATP) levels compared control group (p < 0.05). It also 0.05) reduced damage DNA. However, detrimental observed at resveratrol. conclusion, cryopreservation solution is for enhancing

Language: Английский

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Pyrroloquinoline Quinone Improves Ram Sperm Quality through Its Antioxidative Ability during Storage at 4 °C

Antioxidants, Journal Year: 2024, Volume and Issue: 13(1), P. 104 - 104

Published: Jan. 15, 2024

Sperm motility is an important factor in the migration of sperm from uterus to oviduct. During preservation vitro, generates excessive ROS that damages its function. This study aims investigate whether addition pyrroloquinoline quinone (PQQ) diluted medium could improve chilled ram quality, and then elucidates mechanism. Ram semen was with Tris-citric acid-glucose (TCG) containing different doses PQQ (0 nM, 10 100 1000 10,000 nM), stored at 4 °C. patterns, plasma membrane integrity, acrosome mitochondrial potential, reactive oxygen species (ROS) levels, malondialdehyde (MDA) superoxide dismutase (SOD) activity, ATP levels were measured after preservation. Furthermore, expressions NADH dehydrogenase 1 (MT-ND1) 6 (MT-ND6) also detected by western blotting. In addition, capacitation ability bind zona pellucina evaluated. It observed significantly (p < 0.05) improved motility, integrity during The percentage high potential treatment group much higher than control. supplementation decreased MDA while increasing levels. Interestingly, MT-ND1 MT-ND6 protein treated nM damage proteins. increased tyrosine phosphorylation induced capacitation. value sperm–zona pellucida binding capacity Overall, ram- preservation, protected quality quenching reduce maintain function, preserved sperm’s fertilization.

Language: Английский

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Supplementing Boar Diet with Nicotinamide Mononucleotide Improves Sperm Quality Probably through the Activation of the SIRT3 Signaling Pathway

Antioxidants, Journal Year: 2024, Volume and Issue: 13(5), P. 507 - 507

Published: April 24, 2024

Sperm quality is an important indicator to evaluate the reproduction ability of animals. Nicotinamide mononucleotide (NMN) participates in cell energy metabolism and reduces oxidative stress. However, effect regulatory mechanism NMN on porcine sperm are still unknown. Here, 32 Landrace boars were randomly assigned four groups (n = 8) fed with different levels (0, 8, 16 or mg/kg/d) for 9 weeks, then serum semen samples collected investigate function molecular quality. The results showed that dietary supplementation significantly increased volume, density motility (p < 0.05). Interestingly, apparently improved antioxidative indexes testosterone 0.05) serum. Furthermore, upregulated protein sirtuin 3 (SIRT3), antioxidation phosphorylation (OXPHOS), but downregulated apoptosis semen. Mechanically, protected from H2O2-induced stress through SIRT3 deacetylation. Importantly, SIRT3-specific inhibitor 3-TYP attenuated antiapoptosis sperm. Therefore, exerts improve boar via signaling pathway. Our findings suggest a novel potential feed additive produce high-quality

Language: Английский

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Role of Antioxidants of Natural Herbs in Management of Male Infertility

Journal for Research in Applied Sciences and Biotechnology, Journal Year: 2023, Volume and Issue: 2(1), P. 55 - 80

Published: Feb. 5, 2023

If you and your spouse have been trying to conceive for a year neither of has successful, there is possibility that are one the 50–80 million people throughout world who struggle with infertility. There male component present in approximately 20%-30% these instances. It common knowledge low-quality sperm oxidative stress (OS) both role aetiology Because oxidation DNA, proteins, lipids, excessive levels reactive oxygen species (ROS) potential disrupt viability, motility, morphology cells. ROS responsible changes. Methods: Through use PubMed interface, we searched MEDLINE database studies had completed published during past ten years analysed effect antioxidants on infertile guys (2012–2022). A number different phrases, including infertility, alpha-lipoic acid, zinc, folate, coenzyme Q10, selenium, vitamin, were utilised search. The study's findings indicate inositol serves purpose mechanisms foster contacts between oocytes, it also affects OS cells by way its engagement mitochondrial events. Alpha-lipoic acid (ALA) lessens damage caused improves quality ways, morphology, count. might be connection not getting enough zinc diet having sperm. Zinc folate two nutrients can boost quantity When taken orally, Q10 increases as well their overall motility forward movement. therapy selenium (Se) results an improvement sperm's quality, this connected increase ejaculatory production. Only vitamin B12 found improve sperm; does boosting count decreasing DNA damage. Vitamin only proved do this. Conclusions: Dietary supplementation may men semen reducing OS-induced increasing hormone synthesis spermatozoa concentration, morphology. This achieved low semen. Antioxidants exert effects variety ways; hence, important researchers conducting future clinical trials study combining antioxidants.

Language: Английский

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From oxidative imbalance to compromised standard sperm parameters: Toxicological aspect of phthalate esters on spermatozoa

Environmental Toxicology and Pharmacology, Journal Year: 2023, Volume and Issue: 98, P. 104085 - 104085

Published: Feb. 23, 2023

Language: Английский

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Cuscuta chinensis flavonoids reducing oxidative stress of the improve sperm damage in bisphenol A exposed mice offspring

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 255, P. 114831 - 114831

Published: March 24, 2023

Bisphenol A (BPA) is a common environmental endocrine disruptor, and overexposure threat to male reproduction. Although studies have confirmed that BPA exposure causes decrease in sperm quality offspring, the dosage used, underlying mechanism not clear. The purpose of this study investigate whether Cuscuta chinensis flavonoids (CCFs) can antagonize or alleviate BPA-induced reproductive injury by analyzing processes associated with BPA's impairment quality. 40 mg/kg bw/day CCFs were administered dams at gestation day (GD) 0.5-17.5. Testicles serum mice are collected on postnatal 56 (PND56), spermatozoa detect relevant indicators. Our results showed compared group, could significantly increase contents luteinizing hormone (LH), follicle-stimulating (FSH), testosterone (T) males PND 56, as well transcription levels estrogen receptor alpha (ERα), steroidogenic acute regulatory protein (StAR) Cytochrome P450 family 11, subfamily A, member 1 (CYP11A1). also inhibit production reactive oxygen species (ROS), reduce oxidative stress, mitochondrial membrane potential, apoptosis. It has certain effect telomere length DNA copy number. These suggest adult regulating expression stress correlated factors, ultimately mitigate negative effects mice.

Language: Английский

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Donepezil and quercetin alleviate valproate-induced testicular oxidative stress, inflammation and apoptosis: Imperative roles of AMPK/SIRT1/ PGC-1α and p38-MAPK/NF-κB/ IL-1β signaling cascades

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 134, P. 112240 - 112240

Published: May 13, 2024

Language: Английский

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Sperm Metabolism

Molecular Reproduction and Development, Journal Year: 2024, Volume and Issue: 91(10)

Published: Oct. 1, 2024

ABSTRACT Bioenergetics plays a crucial role in sperm functions, including motility, capacitation‐related protein modifications, oocyte recognition and interaction, all of which are essential for fertilization. Sperm metabolism is recognized as flexible, responding to environmental cues energetic demands during ejaculation, the journey along female tract, until Recent studies suggest that metabolic functions relevant beyond fertilization may influence zygote embryo development, impacting paternal‐derived effects on offspring development health. In recent years, homeostasis have gained increasing interest male reproduction research. Given implications fertility‐related processes, this field not only human fertility but also livestock research, semen conservation, assisted reproductive techniques. Newly developed assessment tools allowing better understanding under different conditions identifying species‐specific peculiarities. This review aims discuss current knowledge mammalian metabolism, focusing features, changes journey, potential contributions translational research biotechnologies. Furthermore, we propose future perspectives bioenergetics

Language: Английский

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