A Validated GC‐MS Method for Major Terpenes Quantification in Hydrodistilled Cannabis sativa Essential oil DOI Creative Commons
Noelle Joy, Daniel Jackson, Timothy Coolong

et al.

Phytochemical Analysis, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Terpenes, which are found in high concentrations the essential oil fraction of Cannabis sativa flower, have demonstrated potential many therapeutic and industrial applications. This work reports on a method developed for quantifying 18 terpenes C. obtained through hydrodistillation. The following has been evaluated specificity, selectivity, accuracy, linearity, precision, stability, limit detection, quantification. Samples were prepared by separating hydrodistillation then diluting with ethyl acetate containing 100 μg/mL solution n-tridecane octadecane as internal standards. Analysis was performed gas chromatograph mass spectrometer (GCMS) using selected ion monitoring (SIM). enabled quantification isomers nerolidol ocimene several coeluting compounds, recoveries 87.35%-116.61%. Two cultivars flower evaluated, dominant terpene compounds both β-myrcene (5.85-8.62 mg/g dried plant) β-caryophyllene (3.89-4.69 mg/g), followed α-humulene (1.35-1.99 limonene (0.91-1.33 α-bisabolol (0.66-0.68 mg/g). provides an accurate reliable procedure major GCMS SIM. simplicity solvent-free nature extraction, combined specificity accuracy SIM external standards, enables determination total individual within plant material supports numerous

Language: Английский

Terpenes and terpenoids as main bioactive compounds of essential oils, their roles in human health and potential application as natural food preservatives DOI Creative Commons
Ayu Masyita, Reka Mustika Sari, Ayun Dwi Astuti

et al.

Food Chemistry X, Journal Year: 2022, Volume and Issue: 13, P. 100217 - 100217

Published: Jan. 19, 2022

Essential oils (EOs) are volatile and concentrated liquids extracted from different parts of plants. Bioactive compounds found in EOs, especially terpenes terpenoids possess a wide range biological activities including anticancer, antimicrobial, anti-inflammatory, antioxidant, antiallergic. Available literature confirms that EOs exhibit antimicrobial food preservative properties considered as real potential application industry. Hence, the purpose this review is to present an overview current knowledge for pharmaceutical medical industries well their preservatives

Language: Английский

Citations

631
A Comprehensive Review on the Techniques for Extraction of Bioactive Compounds from Medicinal Cannabis DOI Creative Commons
H.M.S. Al Ubeed, Deep Jyoti Bhuyan,

Muhammad A. Alsherbiny

et al.

Molecules, Journal Year: 2022, Volume and Issue: 27(3), P. 604 - 604

Published: Jan. 18, 2022

is well-known for its numerous therapeutic activities, as demonstrated in pre-clinical and clinical studies primarily due to bioactive compounds. The

Language: Английский

Citations

89
Cannabis sativa bioactive compounds and their extraction, separation, purification, and identification technologies: An updated review DOI
Yi Liu, Hongyan Liu, Sheng‐Hong Li

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2022, Volume and Issue: 149, P. 116554 - 116554

Published: Feb. 5, 2022

Language: Английский

Citations

71
Plant-Derived Terpenoids: A Plethora of Bioactive Compounds with Several Health Functions and Industrial Applications—A Comprehensive Overview DOI Creative Commons
José S. Câmara, Rosa Perestrelo, Rui Ferreira

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(16), P. 3861 - 3861

Published: Aug. 15, 2024

Terpenoids are a large class of natural secondary plant metabolites which highly diverse in structure, formed from isoprene units (C-5), associated with wide range biological properties, including antioxidant, antimicrobial, anti-inflammatory, antiallergic, anticancer, antimetastatic, antiangiogenesis, and apoptosis induction, considered for potential application the food, cosmetics, pharmaceutical, medical industries. In plants, terpenoids exert variety basic functions growth development. This review gives an overview, highlighting current knowledge recent advances our understanding organization, regulation, diversification core specialized terpenoid metabolic pathways addressing most important volatile non-volatile plants. A comprehensive description different aspects plant-derived as sustainable source bioactive compounds, their biosynthetic pathway, several properties attributed to these health-promoting effects, industrial applications fields will be provided, emerging green extraction methods also discussed. addition, future research perspectives within this framework highlighted. Literature selection was carried out using National Library Medicine, PubMed, international reference data period 2010 2024 keyword "terpenoids". total 177,633 published papers were found, 196 original included according criteria scientific reliability, completeness, relevance theme considered.

Language: Английский

Citations

30
Green and selective supercritical fluid extraction of essential oil and cannabidiol from Cannabis sativa L. DOI Open Access
Lucia Baldino, Mariarosa Scognamiglio, Ernesto Reverchon

et al.

The Canadian Journal of Chemical Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 19, 2025

Abstract In this study, supercritical fluid extraction (SFE) was used to extract cannabis essential oil and cannabinoids from the same matrix by changing decarboxylation conditions CO 2 density. Cannabis extracted at 90 bar 50°C (0.288 g/cm 3 density), it mainly composed of α‐pinene, eucalyptol, β‐caryophyllene, selinene. Subsequently, carried out on vegetable matter operating 105°C for 15 min and, then, 120°C 1 h. The largest cannabidiol (2.96% w/w) yield obtained when SFE performed 100 40°C (0.623 density). An effective online fractional separation compounds also SFE, since no cuticular waxes were detected in GC–MS traces products interest. Operating way, expensive, time‐consuming, polluting post‐processing steps not required, producing a ready‐to‐use market.

Language: Английский

Citations

1
Cannabis for Medical Use: Versatile Plant Rather Than a Single Drug DOI Creative Commons

Shiri Procaccia,

Gil M. Lewitus,

Carni Lipson Feder

et al.

Frontiers in Pharmacology, Journal Year: 2022, Volume and Issue: 13

Published: April 25, 2022

Medical Cannabis and its major cannabinoids (−)- trans -Δ 9 -tetrahydrocannabinol (THC) cannabidiol (CBD) are gaining momentum for various medical purposes as their therapeutic qualities becoming better established. However, studies regarding efficacy oftentimes inconclusive. This is chiefly because a versatile plant rather than single drug effects do not depend only on the amount of THC CBD. Hundreds cultivars hybrids exist worldwide, each with unique distinct chemical profile. Most focus CBD, but these just two over 140 phytocannabinoids found in addition to milieu terpenoids, flavonoids other compounds potential activities. Different plants contain very different array metabolites varying relative ratios, it interplay between molecules from endocannabinoid system body that determines ultimate response associated adverse effects. Here, we discuss how phytocannabinoid profiles differ depending chemovar types, review factors affect secondary metabolite accumulation including genotype, growth conditions, processing, storage delivery route; highlight make treatment highly complex.

Language: Английский

Citations

34
Bioactive compounds from hemp (Cannabis sativa L.) seeds: optimization of phenolic antioxidant extraction using simplex lattice mixture design and HPLC-DAD/ESI-MS2 analysis DOI Creative Commons
Chaymae Benkirane, Abdessamad Ben Moumen, Marie‐Laure Fauconnier

et al.

RSC Advances, Journal Year: 2022, Volume and Issue: 12(39), P. 25764 - 25777

Published: Jan. 1, 2022

The extraction of phenolic compounds from defatted hempseeds was optimized using a simplex lattice mixture design with three solvents (water, methanol, and acetone). response variables were total content (TPC) antioxidant activity evaluated by different spectrophotometric tests. results showed that the binary acetone-water in equal proportions is optimal combination to achieve maximum TPC (53.65 mg GAE per g extract) higher activities (265.53, 36.25, 119.03, 69.46, 68.91 TE g-1 extract for TAC, DPPH, ABTS, FRAP, CUPRAC tests respectively). In addition, profile analysis hemp seeds HPLC-DAD/ESI-MS2 techniques predominance hydroxycinnamic acid amides lignanamides. It allowed visualizing effect each solvent on relative extracted amount identified compound. This study suggests N-trans-caffeoyltyramine, cannabisin A, B might contribute strongly potent hempseed extracts. Thus, it encourages use as source antioxidants added value pharmaceutical cosmetic applications.

Language: Английский

Citations

33
Terpenes and Cannabinoids in Supercritical CO2 Extracts of Industrial Hemp Inflorescences: Optimization of Extraction, Antiradical and Antibacterial Activity DOI Creative Commons
Stela Jokić, Igor Jerković, Valentina Pavić

et al.

Pharmaceuticals, Journal Year: 2022, Volume and Issue: 15(9), P. 1117 - 1117

Published: Sept. 7, 2022

Natural products are increasingly in demand dermatology and cosmetology. In the present study, highly valuable supercritical CO2 (sCO2) extracts rich bioactive compounds with antiradical antibacterial activity were obtained from inflorescences of industrial hemp. Volatile analyzed by gas chromatography tandem mass spectrometry (GC-MS), while cannabinoids determined high performance liquid (HPLC-DAD). Extraction yields varied 0.75 to 8.83%, depending on pressure temperature applied. The extract at 320 bar 40 °C highest content (305.8 µg mg-1) cannabidiolic acid (CBDA) showed best properties. All tested concentrations 10.42 mL-1 66.03 possessed inhibitory activities against E. coli, P. aeruginosa, B. subtilis, S. aureus. sCO2 cannabidiol (CBD) α-pinene, β-pinene, β-myrcene, limonene was most effective. optimal conditions for extraction volatile terpenes hemp determined. 60 proved be all responses studied, a different effect targeted. A low 131.2 monoterpenes, sesquiterpenes 319.7 bar. 284.78 CBD.

Language: Английский

Citations

31
Antimicrobial Properties of Plant Fibers DOI Creative Commons
Lizbeth Zamora-Mendoza, Esteban Guamba,

Karla Miño

et al.

Molecules, Journal Year: 2022, Volume and Issue: 27(22), P. 7999 - 7999

Published: Nov. 18, 2022

Healthcare-associated infections (HAI), or nosocomial infections, are a global health and economic problem in developed developing countries, particularly for immunocompromised patients their intensive care units (ICUs) surgical site hospital areas. Recurrent pathogens HAIs prevail over antibiotic-resistant bacteria, such as methicillin-resistant

Language: Английский

Citations

31
Supercritical Carbon Dioxide Technology for Recovering Valuable Phytochemicals from Cannabis sativa L. and Valorization of Its Biomass for Food Applications DOI Creative Commons
Ana Carolina de Aguiar, Renata Vardanega, Juliane Viganó

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(9), P. 3849 - 3849

Published: May 1, 2023

Supercritical carbon dioxide (CO2) extraction techniques meet all-new consumer market demands for health-promoting phytochemical compound-rich extracts produced from green and sustainable technology. In this regard, review is dedicated to discussing the promise of integrating high-pressure CO2 technologies into Cannabis sativa L. processing chain valorize its valuable pharmaceutical properties food biomass. To do this, cannabis plant, cannabinoids, endocannabinoid system were reviewed understand their therapeutic side effects. The supercritical fluid (SFE) technique was presented as a smart alternative producing bioproducts. impact SFE operating conditions on compound examined aerial parts (inflorescences, stems, leaves), seeds, byproducts. Furthermore, opportunities using non-thermal biomass addressed industrial hemp valorization, focusing biorefinery simultaneously produce cannabidiol new ingredients applications plant-based products.

Language: Английский

Citations

19