Antidiabetic effects of protein hydrolysates from Trachinotus ovatus and identification and screening of peptides with α-amylase and DPP-IV inhibitory activities

Current Research in Food Science, Journal Year: 2023, Volume and Issue: 6, P. 100446 - 100446

Published: Jan. 1, 2023

In the present study, antidiabetic properties of Trachinotus ovatus protein hydrolysates (TOH) in streptozotocin-induced diabetic mice were investigated, and peptides with α-amylase (AAM) dipeptidyl peptidase IV (DPP-IV) inhibitory activities identified screened. The results showed that TOH alleviated body weight loss, polyphagia, blood glucose elevation insulin secretion decline mice. After 4 weeks administration, random (RBG) decreased significantly. groups a dose-dependent reduction fasting (FBG), especially high-dose group, which reduced FBG by 58% versus effect metformin. Moreover, exerted remarkable protective on hepatorenal function, as evidenced increased superoxide dismutase (SOD), catalase (CAT) glutathione peroxidase (GPX) serum urea levels. Histopathological studies confirmed can significantly protect kidney pancreas from histological changes, was great benefit for ensuring normal preventing occurrence complications such nephropathy. Two fractions higher activity against AAM DPP-IV, F4 F6, obtained ultrafiltration TOH-2 (≤3 kDa). A total 19 potentially active 3 F6 screened LC‒MS/MS combined bioinformatic analysis. These are small molecular composed 2-6 amino acids, rich characteristic acids proline, arginine, phenylalanine asparagine, contain high proportions (68% F4, 67% F6) hydrophobicity ≥50%. They offer potent potential could bind to sites internal cavities target enzymes DPP-IV. summary, this study revealed first time effects their derived peptides, promising natural ingredients be used treatment or prevention diabetes.

Language: Английский

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Eucommia ulmoides leaf extracts combined with Astragalus polysaccharides: Effects on growth, antioxidant capacity, and intestinal inflammation in juvenile large yellow croaker (Larimichthys crocea)

Fish & Shellfish Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 110229 - 110229

Published: Feb. 1, 2025

Language: Английский

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Effects of hydroxyproline supplementation in low fish meal diets on collagen synthesis, myofiber development and muscular texture of juvenile Pacific white shrimp (Litopenaeus vannamei)

Animal nutrition, Journal Year: 2024, Volume and Issue: 17, P. 428 - 437

Published: March 28, 2024

This experiment aimed to evaluate the impact of dietary hydroxyproline (Hyp) supplementation on flesh quality juvenile Pacific white shrimp (Litopenaeus vannamei) fed a low fishmeal diet. Six formulated diets included one high (HF; 25% content) and five (10% with 0%, 0.2%, 0.4%, 0.6% 0.8% Hyp (LF0, LF2, LF4, LF6 LF8, respectively). Each diet was assigned four replicates 40 (0.32 ± 0.00 g) per replicate were times day for 8 weeks. Dietary had little effects growth performance, but significantly increased contents Hyp, prolyl 4-hydroxylases (P4Hs), collagen (P < 0.05). The meat yield, springiness, hardness, chewiness, cohesiveness muscle highest in LF2 group. Cooking loss freezing lowest group 0.4% myofiber density decreased diameter Supplementation up-regulated mRNA expression smyhc5, smyhc15, col1a1, col1a2, igf-1f, tgf-β tor, down-regulated smyhc 1, 2, 6a protein P-4E-BP1, P-AKT, AKT P-AKT/AKT These results suggested that addition improved L. vannamei.

Language: Английский

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Comparison of the Retention Rates of Synthetic and Natural Astaxanthin in Feeds and Their Effects on Pigmentation, Growth, and Health in Rainbow Trout (Oncorhynchus mykiss)

Antioxidants, Journal Year: 2022, Volume and Issue: 11(12), P. 2473 - 2473

Published: Dec. 15, 2022

The coloring efficiency and physiological function of astaxanthin in fish vary with its regions. aim this study was to compare the retention rates dietary from different sources effects on growth, pigmentation, Oncorhynchus mykiss. Fish were fed astaxanthin-supplemented diets (LP: 0.1% Lucantin® Pink CWD; CP: Carophyll® Pink; EP: Essention® PR: 1% Phaffia rhodozyma; HP: Haematococcus pluvialis), or a diet without supplementation, for 56 days. Dietary enhanced pigmentation as well growth fish. intestinal morphology improved, crude protein content dorsal muscle significantly increased astaxanthin. Moreover, led decrease total cholesterol levels alanine aminotransferase aspartate activity plasma. CP also produced highest level umami amino acids (aspartic acid glutamic acid). Regarding antioxidant capacity, Nrf2/HO-1 signaling enzyme activity. Innate immune responses, including lysozyme complement systems, stimulated by CWD had stability feed achieved best performed promotion resulted flesh quality. H. pluvialis source achieving properties immunity O.

Language: Английский

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Genome-wide association study for growth-related traits in golden pompano (Trachinotus ovatus)

Aquaculture, Journal Year: 2023, Volume and Issue: 572, P. 739549 - 739549

Published: April 3, 2023

Language: Английский

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Effects of exogenous taurine supplementation on the growth, antioxidant capacity, intestine immunity, and resistance against Streptococcus agalactiae in juvenile golden pompano (Trachinotus ovatus) fed with a low-fishmeal diet

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 14, 2022

Taurine has various biological functions in fish, playing an essential role growth, resistance to oxidative stress, and intestine immunity. Here, we evaluated the effects of exogenous taurine added low-fishmeal diets on anti-oxidative immunity, Streptococcus agalactiae juvenile golden pompano ( Trachinotus ovatus ). Our study showed that supplementation 1.2% (T3 group) greatly enhanced weight gain rate specific growth (SGR) pompano, significantly upregulating growth-related factor expression brain liver, as well levels parameters serum. Polynomial regression analysis using SGR estimated optimal dietary level for at 1.18%. Moderate also increased muscular thickness villus length within intestine, maintained intestinal physical barrier stability, activated Nrf2/Keap-1/HO-1 signaling pathway, antioxidant enzyme gene activity serum, upregulated immunoglobulin complement parallel with declining reactive oxygen species (ROS) Antioxidant was intestine. Furthermore, suppressed NF-κB pro-inflammatory cytokine expression, anti-inflammatory improved Finally, survival challenged S. . Overall, our findings provide additional information support rational use healthy aquatic animal farming.

Language: Английский

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Quercetin alleviates the toxicity of difenoconazole to the respiratory system of carp by reducing ROS accumulation and maintaining mitochondrial dynamic balance

Toxicology and Applied Pharmacology, Journal Year: 2024, Volume and Issue: 484, P. 116860 - 116860

Published: Feb. 10, 2024

Language: Английский

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The effects of citral on the intestinal health and growth performance of American bullfrogs (Aquarana catesbeiana)

BMC Veterinary Research, Journal Year: 2025, Volume and Issue: 21(1)

Published: Feb. 3, 2025

Bullfrogs (Aquarana catesbeiana) are increasingly cultivated for their high nutritional value and adaptability to intensive aquaculture systems. However, ensuring optimal intestinal health growth performance remains a challenge due poor water quality stocking densities. This study evaluated the effects of varying dietary concentrations citral, natural compound from lemongrass essential oil, on health, microbiota, antioxidant capacity, juvenile bullfrogs. A total 200 bullfrogs (initial weight 6.85 ± 0.71 g) were randomly assigned into six groups, each receiving diets supplemented with citral at 0, 1, 2, 4, 8, 16 mL/kg feed 8 weeks. Citral supplementation significantly improved morphology, goblet cell numbers, mucosal thickness, villus-to-crypt ratios peaking 2-4 (P < 0.05). Optimal doses also enhanced digestive enzyme activities, α-amylase, lipase, pepsin activities showing significant increases compared control group Antioxidant markers, including capacity (T-AOC) glutathione (GSH), highest 2 mL/kg, while higher reduced superoxide dismutase (SOD) catalase (CAT) indicating potential oxidative stress 8-16 modulated increasing relative abundance beneficial bacteria such as Cetobacterium 1-2 microbial diversity decreased above 4 mL/kg. Growth analysis revealed that gain rate (WGR), specific (SGR), carcass (CW), efficiency (FE), survival rates declined linear regression model determined concentration be 3.216-3.942 concludes optimally enhances performance, in bullfrogs, may disrupt gut balance. These findings provide valuable insights use compounds like sustainable practices.

Language: Английский

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Continuous Liquid-Liquid Extraction of High-Purity Lutein from Chlorella Vulgaris Via Centrifugal Partition Chromatography: Utilizing Limonene as Renewable Solvent for Microalgae Biomass Valorization

Published: Jan. 1, 2025

Language: Английский

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Continuous Liquid–Liquid Extraction of High-Purity Lutein from Chlorella vulgaris via Centrifugal Partition Chromatography: Utilizing Limonene as Renewable Solvent for Microalgae Biomass Valorization

Foods, Journal Year: 2025, Volume and Issue: 14(9), P. 1637 - 1637

Published: May 6, 2025

In this study, an efficient and eco-friendly method was developed for continuous liquid–liquid extraction of lutein from microalgae Chlorella vulgaris. By employing a limonene-based biphasic liquid system, high-purity successfully obtained the crude extract in single run via centrifugal partition chromatography (CPC). Evaluation optimization results demonstrated that limonene could effectively serve as replacement n-hexane solvent system extraction, exhibiting natural renewability minimal environmental impact. Furthermore, elution–extrusion operation mode employed to fully exploit nature stationary phase process, allowing sampling separation without interruption. This proposed protocol offers sustainable environmentally friendly alternative extracting valuable ingredients biomass, demonstrating its potential scalable solution producing lutein-enriched applicable functional foods nutraceuticals.

Language: Английский

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