Lambertianic Acid from Platycladus orientalis Inhibits Muscle Atrophy in Dexamethasone-Induced C2C12 Muscle Atrophy Cells DOI Creative Commons

Chan Hee Cho,

Si Hyeon Chae,

Ngoc Han Le Thi

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(9), P. 1357 - 1357

Published: April 30, 2025

Platycladus orientalis, an evergreen tree belonging to the Cupressaceae family, has been traditionally used treat various ailments, including fever, cough, diarrhea, diuresis, cold symptoms, and gastrointestinal disorders in folk medicine. As part of our ongoing investigation aimed at discovering bioactive natural products elucidating their mechanisms action from sources, we investigated a methanol (MeOH) extract P. orientalis leaves. This led isolation identification labdane-type diterpene, lambertianic acid (LA), via column chromatography HPLC purification. The structure LA was elucidated using LC/MS NMR spectroscopic analyses, HR-ESIMS, while its absolute configuration confirmed through electronic circular dichroism (ECD) calculations. Recent studies have reported that diterpenes exhibit diverse pharmacological activities, such as anticancer, anti-inflammatory, anti-obesity, hypolipidemic effects. Notably, shown modulate adipocyte metabolism AMPK signaling; however, role skeletal muscle atrophy remains unexplored. Therefore, this study, effects on dexamethasone (Dex)-induced C2C12 myotubes. Treatment with concentrations 25 µM 50 significantly rescued myotube diameter reduced expression atrophy-related proteins, MuRF-1 atrogin-1/MAFbx, without compromising cell viability these moderate concentrations. These findings suggest derived exerts protective against atrophy, highlighting potential promising therapeutic candidate for muscle-wasting disorders.

Language: Английский

Mitochondrial dysfunction: roles in skeletal muscle atrophy DOI Creative Commons
Xin Chen, Yanan Ji, Ruiqi Liu

et al.

Journal of Translational Medicine, Journal Year: 2023, Volume and Issue: 21(1)

Published: July 26, 2023

Abstract Mitochondria play important roles in maintaining cellular homeostasis and skeletal muscle health, damage to mitochondria can lead a series of pathophysiological changes. Mitochondrial dysfunction atrophy, its molecular mechanism leading atrophy is complex. Understanding the pathogenesis mitochondrial useful for prevention treatment finding drugs methods target modulate function are urgent tasks atrophy. In this review, we first discussed normal muscle. Importantly, described effect on mechanisms involved. Furthermore, regulatory different signaling pathways (AMPK-SIRT1-PGC-1α, IGF-1-PI3K-Akt-mTOR, FoxOs, JAK-STAT3, TGF-β-Smad2/3 NF-κB pathways, etc.) factors were investigated dysfunction. Next, analyzed manifestations caused by diseases. Finally, summarized preventive therapeutic effects targeted regulation including drug therapy, exercise diet, gene stem cell therapy physical therapy. This review great significance holistic understanding role muscle, which helpful researchers further has an inspiring development strategies targeting future.

Language: Английский

Citations

114
Oxidative stress: Roles in skeletal muscle atrophy DOI
Han Zhang,

Guangdong Qi,

Kexin Wang

et al.

Biochemical Pharmacology, Journal Year: 2023, Volume and Issue: 214, P. 115664 - 115664

Published: June 16, 2023

Language: Английский

Citations

97
Fiber-Type Shifting in Sarcopenia of Old Age: Proteomic Profiling of the Contractile Apparatus of Skeletal Muscles DOI Open Access
Paul Dowling, Stephen Gargan, Dieter Swandulla

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2415 - 2415

Published: Jan. 26, 2023

The progressive loss of skeletal muscle mass and concomitant reduction in contractile strength plays a central role frailty syndrome. Age-related neuronal impairments are closely associated with sarcopenia the elderly, which is characterized by severe muscular atrophy that can considerably lessen overall quality life at old age. Mass-spectrometry-based proteomic surveys senescent human muscles, as well animal models sarcopenia, have decisively improved our understanding molecular cellular consequences fiber-type shifting during aging. This review outlines spectrometric identification proteome-wide changes atrophying focus on proteins potential markers distribution patterns. observed trend fast-to-slow transitions individual muscles aging process most likely linked to preferential susceptibility fast-twitching fibers atrophy. Studies models, including mostly aged rodent confirmed shifting. analysis fast versus slow isoforms key proteins, such myosin heavy chains, light actins, troponins tropomyosins, suggests them suitable bioanalytical tools

Language: Английский

Citations

46
Endoplasmic reticulum stress and unfolded protein response: Roles in skeletal muscle atrophy DOI
Yanan Ji, Quan Jiang,

Bingqian Chen

et al.

Biochemical Pharmacology, Journal Year: 2025, Volume and Issue: 234, P. 116799 - 116799

Published: Feb. 12, 2025

Language: Английский

Citations

2
Duchenne muscular dystrophy: pathogenesis and promising therapies DOI

Mengyuan Chang,

Yong Cai,

Zihui Gao

et al.

Journal of Neurology, Journal Year: 2023, Volume and Issue: 270(8), P. 3733 - 3749

Published: June 1, 2023

Language: Английский

Citations

27
Celecoxib attenuates hindlimb unloading-induced muscle atrophy via suppressing inflammation, oxidative stress and ER stress by inhibiting STAT3 DOI
Yanan Ji,

Junfei Lin,

Ruiqi Liu

et al.

Inflammopharmacology, Journal Year: 2024, Volume and Issue: 32(2), P. 1633 - 1646

Published: March 7, 2024

Language: Английский

Citations

9
hBMSC-EVs alleviate weightlessness-induced skeletal muscle atrophy by suppressing oxidative stress and inflammation DOI Creative Commons

Mengyuan Chang,

Ruiqi Liu,

Bingqian Chen

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 4, 2025

Muscle disuse and offloading in microgravity are likely the primary factors mediating spaceflight-induced muscle atrophy, for which there is currently no effective treatment other than exercise. Extracellular vesicles derived from bone marrow mesenchymal stem cells (BMSC-EVs) possess anti-inflammatory antioxidant properties, offering a potential strategy combating weightless muscular atrophy. In this study, human BMSCs-EVs (hBMSC-EVs) were isolated using super-centrifugation characterized. C2C12 myotube nutrition-deprivation mice tail suspension models established. Subsequently, diameter of myotubes, Soleus mass, cross-sectional area (CSA) fibers, grip strength assessed to investigate impact hBMSC-EVs on Immunostaining, transmission electron microscopy observation, western blot analysis employed assess fiber types, ROS levels, inflammation, ubiquitin–proteasome system activity, autophagy lysosome pathway activation skeletal The active can be internalized by myotubes muscle. effectively reduce atrophy caused nutritional deprivation, with concentration 10 × 108 particles/mL showing best effect (P < 0.001). Additionally, down-regulate protein levels associated UPS oxidative stress. Moreover, intravenous administration at 1 1010 reverse reduction soleus mass 0.001), CSA 0.01), 0.001) weightlessness. They demonstrate ability inhibit degradation mediated pathway, along suppression stress inflammatory responses. Furthermore, impede transition slow fibers fast via upregulation Sirt1 PGC-1α levels. Our findings indicate that capable inhibiting excessive suppressing response, reversing type transformation, delaying hindlimb unloading-induced enhancing function. study has further advanced understanding molecular mechanism underlying weightlessness demonstrated protective

Language: Английский

Citations

1
Licochalcone A and B enhance muscle proliferation and differentiation by regulating Myostatin DOI Creative Commons
Khurshid Ahmad, Eun Ju Lee, Shahid Ali

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 125, P. 155350 - 155350

Published: Jan. 9, 2024

Myostatin (MSTN) inhibition has demonstrated promise for the treatment of diseases associated with muscle loss. In a previous study, we discovered that Glycyrrhiza uralensis (G. uralensis) crude water extract (CWE) inhibits MSTN expression while promoting myogenesis. Furthermore, three specific compounds G. uralensis, namely liquiritigenin, tetrahydroxymethoxychalcone, and Licochalcone B (Lic B), were found to promote myoblast proliferation differentiation, as well accelerate regeneration injured tissue. The purpose this study was build on our findings demonstrate potential its two components, A A) Lic B, in mass regulation (by inhibiting MSTN), aging formation. A, evaluated thoroughly using silico, vitro vivo approaches. silico analyses included molecular docking, dynamics simulations these MSTN. Protein-protein docking carried out MSTN, docked complex MSTN-Lic receptor, activin type IIB receptor (ACVRIIB). Subsequent studies used C2C12 cell lines primary mouse stem cells acess differentiation normal aged cells, levels Atrogin 1, MuRF1, plasma concentrations, employing techniques such western blotting, immunohistochemistry, immunocytochemistry, assays, real-time RT-PCR. experiments models focused measuring fiber diameters. CWE by reducing Atrogin1 MuRF1 expressions protein concentration serum. interaction analysis revealed (binding energy -6.9 Kcal/mol) -5.9 bind reduce binding between it ACVRIIB, thereby downstream signaling. experimental analysis, which involved both studies, decreased when CWE, or administered into mice treated satellite (MSCs) myoblasts. diameters fibers increased orally mice, enhanced. also promoted suggesting they may have anti-muslce properties. They reduced phosphorylation SMAD2 SMAD3 (MSTN effectors), adding evidence is inhibited. These suggest active constituents anti-mauscle potential. be natural inhibitors therapeutic options disorders atrophy.

Language: Английский

Citations

8
MuSCs and IPCs: roles in skeletal muscle homeostasis, aging and injury DOI Creative Commons
Haiyan Jiang, Boya Liu,

Junfei Lin

et al.

Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)

Published: Jan. 30, 2024

Abstract Skeletal muscle is a highly specialized tissue composed of myofibres that performs crucial functions in movement and metabolism. In response to external stimuli injuries, range stem/progenitor cells, with stem cells or satellite (MuSCs) being the predominant cell type, are rapidly activated repair regenerate skeletal within weeks. Under normal conditions, MuSCs remain quiescent state, but become proliferative differentiate into new injury. addition MuSCs, some interstitial progenitor (IPCs) such as fibro-adipogenic progenitors (FAPs), pericytes, expressing PW1 negative for Pax7 (PICs), side population (SPCs), CD133-positive Twist2-positive have been identified playing direct indirect roles regenerating tissue. Here, we highlight heterogeneity, molecular markers, functional properties these explore role homeostasis, aging, muscle-related diseases. This review provides critical insights future therapies aimed at treating

Language: Английский

Citations

8
Skeletal muscle atrophy after sciatic nerve damage: Mechanistic insights DOI
Aarti Yadav, Rajesh Dabur

European Journal of Pharmacology, Journal Year: 2024, Volume and Issue: 970, P. 176506 - 176506

Published: March 15, 2024

Language: Английский

Citations

7