Sourdough Fermentation Improves the Antioxidant, Antihypertensive, and Anti-Inflammatory Properties of Triticum dicoccum

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(7), P. 6283 - 6283

Published: March 27, 2023

The fermentation process has been widely used to improve plant-based foods' nutritional and nutraceutical properties. This study aimed investigate compare the impact of sourdough on bioactive content profile, antioxidant antihypertensive activities, as well anti-inflammatory properties fermented (FS) non-fermented (NFS) flour from Tuscan Triticum dicoccum wheat (spelt) tumor necrosis factor-alpha (TNF-α)-inflamed human intestinal epithelial cells (HT-29). FS showed significantly higher total phenolic flavonoid content, in vitro ex vivo ACE-inhibitory activities than NFS. Gallic acid was identified by HPLC-DAD most representative polyphenol, followed rutin, trans-ferulic acid, iso-quercitrin, quercetin, spelt sample. Instead, rutin gallic were predominant compounds ones. Moreover, exhibited a better protective effect inflamed HT-29 counteracting TNFα-induced alterations, lowering expression IL-8, COX-2, ICAM-1 inflammatory mediator while enhancing enzyme HO-1 gene expression. In conclusion, positively affected functional spelt, which may represent valuable ingredient for formulation foods key product managing hypertension diseases.

Language: Английский

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Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties

Antioxidants, Journal Year: 2023, Volume and Issue: 12(7), P. 1424 - 1424

Published: July 14, 2023

The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, lipid fraction prepared for quantifying fatty acids using GC-FID. Extractable alkaline-hydrolysable PBP fractions (obtained defatted sample) were used determine qualitative quantitative profiles phenolic compounds, phenylamides alkaloids UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) conducted evaluate properties. Phenolic compounds more present in extractable than fraction. Luteolin predominant compound fraction, followed by tricetin derivatives kaempferol. This presents one first reports on quantification aglycone outside Myrtaceae plant family. exhibited different profile, with significantly lower amount phenolics. Kaempferol/derivatives, specific like ferulic 5-carboxyvanillic acids, (epi)catechin 3-O-gallate Regarding phenylamides, demonstrated diverse range these bioactive notable abundance spermine derivatives. In contrast, hydrolysable contained six spermidine derivative. examined also revealed presence seventeen alkaloids, belonging benzylisoquinoline, berberine isoquinoline classes. fatty-acid confirmed prevalence unsaturated acids. Furthermore, both significant activity, showing particularly high activity. Among conducted, CUPRAC assay highlighted exceptional ability PBP’s reduce cupric ions.

Language: Английский

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Translational Research on Bee Pollen as a Source of Nutrients: A Scoping Review from Bench to Real World

Nutrients, Journal Year: 2023, Volume and Issue: 15(10), P. 2413 - 2413

Published: May 22, 2023

The emphasis on healthy nutrition is gaining a forefront place in current biomedical sciences. Nutritional deficiencies and imbalances have been widely demonstrated to be involved the genesis development of many world-scale public health burdens, such as metabolic cardiovascular diseases. In recent years, bee pollen emerging scientifically validated candidate, which can help diminish conditions through nutritional interventions. This matrix being extensively studied, has proven very rich well-balanced nutrient pool. this work, we reviewed available evidence interest source. We mainly focused richness nutrients its possible roles main pathophysiological processes that are directly linked imbalances. scoping review analyzed scientific works published last four focusing clearest inferences perspectives translate cumulated experimental preclinical into clinically relevant insights. promising uses for malnutrition, digestive health, disorders, other bioactivities could helpful readjust homeostasis (as it also true case anti-inflammatory or anti-oxidant needs), well benefits diseases, were identified. knowledge gaps identified, along with practical challenges hinder establishment fructification these uses. A complete data collection made major range botanical species allows more robust clinical information.

Language: Английский

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Phenolic Screening and Mixture Design Optimization for In Vitro Assessment of Antioxidant and Antimicrobial Activities of Honey, Propolis, and Bee Pollen

Journal of Food Biochemistry, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

The current study used a design of experiments to evaluate the potential synergistic effects three Moroccan bee products (honey (H), propolis (P), and pollen (BP)) on free radical inhibition antibacterial activity against clinical strains Staphylococcus aureus Escherichia coli . phytochemical contents these were first evaluated using HPLC‐DAD with 20 identified compounds (9 in both H P extracts 13 BP extract). extract had highest content, high levels flavanone pinocembrin, flavanol catechin, lignan pinoresinol, simple phenolics ( p ‐coumaric gallic acids). Then, optimized mixtures determined an augmented simplex‐centroid design. formulations (H33%:P43%:BP24%) (H21%:P47%:BP32%) presented optimal total phenolic content DPPH‐IC 50 226.88 mgGAE/g 10.64 µg /mL, respectively, whereas (H26%:P52%:BP22%) (H35%:P40%:BP25%) showed antimicrobial S. (MIC S.aureus equal 4.34 µ g/mL) E. E.coli 5.70 g/mL), respectively. predicted responses from mixture proportions also experimentally validated. Compared single effect each isolated product, demonstrated increased biological activity, determination fractional inhibitory concentrations revealed between products. This emphasizes interest product for practical applications beyond pharmaceutical food industries. Their can be extended nutraceuticals, cosmeceuticals, animal health, environmental sustainability, advanced biomedical research, offering holistic solutions diverse challenges across various sectors. Exploring further unlock new avenues innovation sustainable development.

Language: Английский

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Antioxidant-Rich Polyfloral Bee Pollen Exerts Antimicrobial Activity and Anti-Inflammatory Effect in A549 Lung Epithelial Cells by Modulating the NF-κB Pathway

Foods, Journal Year: 2025, Volume and Issue: 14(5), P. 802 - 802

Published: Feb. 26, 2025

Bee pollen is produced by honeybees from the agglutination of grains belonging to one or more plant species. Although it intended be a nutritional source for hive, its remarkable concentration nutrients and phytochemicals, combined with pleasant organoleptic qualities, makes appealing human consumption. This study examined phytochemical content antioxidant antimicrobial activities polyfloral bee collected in Tuscany (Italy). Additionally, less studied anti-inflammatory potential towards tumor necrosis factor-alpha (TNF-α)-inflamed A549 cells was evaluated assess possible use inflammatory respiratory diseases. extract (BPE) chemically characterized terms total polyphenol (20.2 ± 1.5 mg gallic acid equivalents/g fw), flavonoid (9.22 0.64 quercetin carotenoid (10.4 1.4 µg carotenoids/g fw) contents. BPE exerted good antiradical ferric reducing power (38.6 4.5 Fe2+/g oxygen radical absorbance capacity (433.77 18.95 μmol TE/g 2,2-diphenyl-1-picrylhydrazyl (EC50 = 613.8 79.2 μg/mL) assays. inhibited growth Bacillus subtilis Pseudomonas stutzeri (MIC 10 mg/mL) microdilution assay. When TNF-α-inflamed were pretreated (10 50 µg/mL), upregulated interleukin-8 gene cyclooxygenase-2 protein expression significantly attenuated. modulated nuclear factor-κB signaling pathway decreasing active phosphorylated form levels. These encouraging results confirm that honeybee valuable health-promoting food could alleviate component various chronic pulmonary conditions.

Language: Английский

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Comparative effects of glucosinolate-derived isothiocyanates from Camelina sativa seeds and sulforaphane on A549 lung cancer cells.

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: unknown, P. 101982 - 101982

Published: May 1, 2025

Language: Английский

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Assessment of Sourdough Fermentation Impact on the Antioxidant and Anti-Inflammatory Potential of Pearl Millet from Burkina Faso

Foods, Journal Year: 2024, Volume and Issue: 13(5), P. 704 - 704

Published: Feb. 26, 2024

Millet, a gluten-free cereal, has received attention for its environmental friendliness and higher protein content than other grains. It represents staple food in many African countries, where fermentation is traditionally used preserving products preparing different cereal-based products. This study aimed to assess the impact of sourdough on bioactive compounds antioxidant anti-inflammatory properties pearl millet from Burkina Faso. Phenolic were investigated spectrophotometrically by HPLC-DAD. The activity unfermented (MF) fermented (FeMF) was evaluated vitro spectrophotometric fluorometric assays ex vivo oxidized human erythrocytes hemolysis inhibition. Finally, potential effect FeMF MF adenocarcinoma cell line (HT-29) exposed TNF-α inflammatory stimulus. Results revealed significantly levels polyphenols, flavonoids, following fermentation. Notable differences phenolic composition between are observed, with facilitating release such as gallic acid, quercetin, rutin. A dose-dependent protection against oxidative observed both FeMF- MF-pretreated erythrocytes. Similarly, pretreatment reduced markers TNF-α-treated cells, effects comparable those MF. Fermentation simple low-cost method improve flour promising nutraceutical potential.

Language: Английский

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Exploring microalgae and endophyte as biostimulants: antioxidant and anti-inflammatory properties of Cannabis sativa L. sprouts under standard and enrichment conditions

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105138 - 105138

Published: Sept. 1, 2024

Language: Английский

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67th International Open Readings Conference for students of Physics and Natural Sciences. Annual Abstract Book 2024

Vilnius University Proceedings, Journal Year: 2024, Volume and Issue: 46, P. 1 - 410

Published: May 31, 2024

“Open Readings 2024” is an international academic platform for students and young researchers worldwide to present their ongoing research results practices in physics natural sciences. The 67th International Scientific Conference Students of Physics Natural Sciences brings together participants discuss, learn, exchange, share scientific experiences, meet key experts, enjoy exciting program. conference features lectures by well-known, leading, accomplished scientists, as well presentations students’ research. offers BSc, MSc, PhD opportunity a larger audience receive constructive criticism helpful advice. This book provides overview all the at Open 2024 conference.

Language: Английский

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Anti-proliferative effect and mechanisms of Peony pollen on BPH via inhibition of inflammatory factors, oxidative damage and modulation of gut microbiota and SCFAs metabolism

Pharmacological Research - Modern Chinese Medicine, Journal Year: 2024, Volume and Issue: 12, P. 100472 - 100472

Published: June 21, 2024

Peony pollen (PP) has long been used as a functional food and herbal medicine. However, the potential protective effect mechanisms of PP on benign prostatic hyperplasia (BPH) are still unclear. This study aims to explore therapeutic BPH. The rat model BPH was induced by subcutaneous injection testosterone propionate (TP). Meanwhile, rats were treated with PP, physiological biochemical indexes, steroid 5 alpha reductase 2 (SRD5A2), proliferating cell nuclear antigen (PCNA) androgen receptor (AR) expressions, gut microbiota short-chain fatty acids (SCFAs) measured. results showed that wet weight prostate tissue thickness epithelium decreased significantly. In addition, also adjusted levels, down-regulated SRD5A2, PCNA AR inhibited expression inflammatory factors including interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), 1β (IL-1β) reduced oxidative damage in rats. It is worth noting can obviously regulate disorder rats, increase abundance beneficial bacteria (e.g., Romboutsia), reduce pathogenic norank_f_Oscillospiraceae), stimulate SCFAs content. These data resist abnormal proliferation down regulation attenuating oxidation stress damages directly microbiota, especially Firmicutes, increased level ameliorate Therefore, might be an excellent natural plant for development BPH-related drugs or dietary supplements.

Language: Английский

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