Enhancing Tetrahydrocannabinol’s Therapeutic Efficacy in Inflammatory Bowel Disease: The Roles of Cannabidiol and the Cannabinoid 1 Receptor Allosteric Modulator ZCZ011

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(2), P. 148 - 148

Published: Jan. 23, 2025

Background/Objectives: Current inflammatory bowel disease (IBD) treatments focus on symptomatic relief, highlighting the need for innovative approaches. Dysregulation of cannabinoid 1 (CB1) receptor, part endocannabinoid system, is linked to colitis. While tetrahydrocannabinol (THC) alleviates colitis via CB1 activation, its psychotropic effects limit clinical use. ZCZ011, a CB1R allosteric modulator, and cannabidiol (CBD), non-psychoactive cannabinoid, offer alternatives. This study investigated combining sub-therapeutic THC doses with ZCZ011 or CBD in murine model dextran sodium sulphate (DSS)-induced Methods: Acute was induced 4% DSS 7 days, followed by 3 days water. Chronic modelled over 24 alternating concentrations. The combination 2.5 mg/kg 20 10 evaluated. Key markers were assessed determine efficacy safety, including activity index (DAI), inflammation, cytokine levels, GLP-1, organ health. Results: DSS-induced resulted increased DAI scores, cytokines, inflammation dysregulation GLP-1 ammonia. at significantly improved but ineffective 5 mg/kg. alone showed transient effects. However, either alleviated markers, restored colon integrity reestablished homeostasis. also maintained favourable haematological biochemical profiles, notable reduction colitis-induced elevated ammonia levels. Conclusions: demonstrates synergistic potential low-dose combined as novel, effective safer therapeutic strategy ulcerative

Language: Английский

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The Skin and Natural Cannabinoids–Topical and Transdermal Applications

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(7), P. 1049 - 1049

Published: July 24, 2023

The chemical constituents of the

Language: Английский

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Determining Antioxidant Activity of Cannabis Leaves Extracts from Different Varieties—Unveiling Nature’s Treasure Trove

Antioxidants, Journal Year: 2023, Volume and Issue: 12(7), P. 1390 - 1390

Published: July 6, 2023

Cannabis leaves contain a diverse range of antioxidants, including cannabinoids, flavonoids, and phenolic compounds, which offer significant health benefits. Utilising cannabis as source antioxidants presents cost-effective approach because they are typically discarded during the cultivation plants for their seeds or fibres. Therefore, this presented study aimed to assess antioxidant activity selected hemp cultivars, such Białobrzeska, Tygra, Henola, based on results obtained with 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid, ferric reducing power, cupric capacity, 2,2-Diphenyl-1-picrylhydrazyl assays. The cannabinoid profile was analysed contents cannabidiol (CBD), cannabigerol (CBG), Δ9-tetrahydrocannabinol (Δ9-THC), cannabichromene (CBC), determined chromatographic following variables were tested: impact various extractants (methanol, ethanol, isopropanol), mixtures (50:50, v/v, well extraction methods (maceration ultra-sound-assisted extraction) in obtaining extracts characterised by different profiles. revealed that selection extractant conditions significantly influenced active compounds' efficiency activity. Among tested conditions, ultrasound-assisted using methanol yielded highest profile: CBD = 184.51 ± 5.61; CBG 6.10 0.21; Δ9-THC 0.51 0.01; CBC 0.71 0.01 μg/g potential Białobrzeska leaf extracts.

Language: Английский

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Cannabidiol-Loaded Solid Lipid Nanoparticles Ameliorate the Inhibition of Proinflammatory Cytokines and Free Radicals in an In Vitro Inflammation-Induced Cell Model

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4744 - 4744

Published: April 26, 2024

Cannabidiol (CBD) is a non-psychoactive compound derived from Cannabis sativa. It has demonstrated promising effects in combating inflammation and holds potential as treatment for the progression of chronic inflammation. However, clinical application CBD limited due to its poor solubility bioavailability. This study introduces an effective method preparing CBD-loaded solid lipid nanoparticles (CBD-SLNs) using combination low-energy hot homogenization ultrasonication. We enhanced this process by employing statistical optimization with response surface methodology (RSM). The optimized CBD-SLN formulation utilizes glyceryl monostearate primary component nanocarrier. screened tool managing Stable, uniformly dispersed spherical size 123 nm, charge −32.1 mV, encapsulation efficiency 95.16%, drug loading 2.36% were obtained. CBD-SLNs exhibited sustained release properties, ensuring prolonged controlled delivery, which could potentially amplify therapeutic effects. Additionally, we observed that significantly reduced both reactive oxygen nitrogen species proinflammatory cytokines chondrocyte macrophage cell lines, these inhibitory being more pronounced than those free CBD. In conclusion, superiority over CBD, highlighting delivery system

Language: Английский

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Cannabidiol and Beta-Caryophyllene Combination Attenuates Diabetic Neuropathy by Inhibiting NLRP3 Inflammasome/NFκB through the AMPK/SIRT3/Nrf2 Axis

Published: April 28, 2024

Background In this study we investigated in detail the role of cannabidiol (CBD), Beta-caryophyllene (BC), or their combinations diabetic peripheral neuropathy (DN). The key factors that contribute to DN include mitochondrial dysfunction, inflammation, and oxidative stress. Methods Briefly, Streptozotocin (STZ) (55 mg/kg) was injected intraperitoneally induce Sprague Dawley rats performed procedures including Randall Sellito Calipers, Von Frey Aesthesiometer, hot plate, cold plate methods determine mechanical thermal hyperalgesia vivo. blood flow nerves assessed by using Laser Doppler device. Schwann cells were exposed high glucose (HG) at a dose 30 mM hyperglycemia DCFDA, JC1 & Mitosox staining done membrane potential, reactive oxygen species superoxides in-vitro. administered i.p with BC (30mg/kg), CBD (15mg/kg), combination while treated 3.65 µM CBD, 75 BC, assess amelioration. Results Our results revealed exposure diminished HG-induced cells, part, through reducing superoxides. Further, treatment in-vivo could prevent deterioration quality control system promoting autophagy biogenesis improving flow. treatments also reduced pain hypersensitivity allodynia increased antioxidant anti-inflammatory action rats. These vivo effects attributed significant upregulation AMPK, SIRT3, Nrf2, PINK1, PARKIN, LC3B, Beclin1 TFAM functions downregulation NLRP3 inflammasome, NFκB, COX2 p62 activity noted as determined western blotting. Conclusion present studies demonstrated STZ HG induced nitrosative stress play crucial pathogenesis neuropathy. We find, for first time, ameliorate via modulating system.

Language: Английский

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Cannabidiol and Beta-Caryophyllene Combination Attenuates Diabetic Neuropathy by Inhibiting NLRP3 Inflammasome/NFκB through the AMPK/sirT3/Nrf2 Axis

Biomedicines, Journal Year: 2024, Volume and Issue: 12(7), P. 1442 - 1442

Published: June 28, 2024

In this study, we investigated in detail the role of cannabidiol (CBD), beta-caryophyllene (BC), or their combinations diabetic peripheral neuropathy (DN). The key factors that contribute to DN include mitochondrial dysfunction, inflammation, and oxidative stress.

Language: Английский

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Effects of Oral Cannabidiol on Health and Fitness in Healthy Adults: An 8-Week Randomized Trial

Nutrients, Journal Year: 2023, Volume and Issue: 15(12), P. 2664 - 2664

Published: June 7, 2023

There is a lack of research on the effects cannabidiol (CBD) health-related fitness, physical activity, cognitive health, psychological wellbeing, and concentrations C-reactive protein (CRP) in healthy individuals. CBD has potential anti-inflammatory neuroprotective effects.

Language: Английский

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Kinetic studies of the rich interaction of cannabinoids with oxygen-centered radicals and excited carbonyl compounds

Free Radical Biology and Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

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Targeting Epigenetic Plasticity to Reduce Periodontitis-Related Inflammation in Diabetes: CBD, Metformin, and Other Natural Products as Potential Synergistic Candidates for Regulation? A Narrative Review

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2853 - 2853

Published: March 21, 2025

Periodontitis is unanimously accepted to be the sixth complication of diabetes mellitus (DM), while inverse relationship causality still deciphered. Among proposed mechanisms gut dysbiosis, which responsible for systemic release proinflammatory mediators. In this process, Gram-negative bacteria from oral cavity enter general circulation, leading emergence bi-hormonal beta-pancreatic cells that lack ability secrete insulin. Additionally, epigenetic and adaptive in affected may play a role reducing inflammation. The reactive oxygen species, cytokines, adipokines, such as interleukins, tumor necrosis factor alpha, leptin, prostaglandin E2, C-reactive protein, or matrix metalloproteinases, determine changes, methylation DNA nucleotides changes activity histone acetylases/deacetylases. management periodontitis involves targeting inflammation, its potential connection modulation observed other chronic conditions help explain preventing DM patients. This review focuses on key might contribute development, explores novel multi-drug therapies could prevent these effects.

Language: Английский

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Effect of Different Extraction Methods on the Structural Analysis, Water Solubility, and Bioactivity of Hemp Polysaccharides-Cannabidiol Complexes

Colloids and Surfaces A Physicochemical and Engineering Aspects, Journal Year: 2025, Volume and Issue: unknown, P. 136729 - 136729

Published: March 1, 2025

Language: Английский

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