Effects of rearing systems (cage versus floor) on the microbial composition and transcriptome of goose ileum DOI Creative Commons

Zhiyu He,

Xuejian Li, Xi Zhang

et al.

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: May 23, 2024

There is a gradual transition from water to dryland rearing of geese. In this study, we performed 16S rRNA sequencing (16S rRNA-seq) and transcriptome (RNA-seq) reveal the effects cage (CR) floor (FR) systems on microbial composition goose ileum. Through rRNA-seq, Linear Discriminant Analysis Effect Size (LEfSe) analysis identified 2 ( hgcI_clade Faecalibacterium ) 14 Bacteroides , Proteiniphilum Proteiniclasticum etc.) differential microbiota in CR FR, respectively. The system influenced 4 pathways including biosynthesis amino acids ileal microbiota. Moreover, 1,198 differentially expressed genes (DEGs) ileum mucosa, with 957 up-regulated 241 FR. CR, Kyoto Encyclopedia Genes Genomes (KEGG) pathway revealed significant enrichment p < 0.05) 28 KEGG pathways, most which were associated acid metabolism. DEGs mainly enriched cellular processes, apoptosis, necroptosis, senescence. Spearman correlation metabolism-related showed positive correlation. Additionally, Phascolarctobacterium Sutterella positively correlated FGF10 PIK3R1 0.01), conclusion, there might be differences metabolism levels between FR geese, observed increase harmful bacterial species impact activity cells.

Language: Английский

Effects of Heat-Treated Lactobacillus helveticus CP790-Fermented Milk on Gastrointestinal Health in Healthy Adults: A Randomized Double-Blind Placebo-Controlled Trial DOI Open Access
Reiko Tanihiro, Masahiro Yuki,

Katsuhisa Sakano

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(14), P. 2191 - 2191

Published: July 10, 2024

Probiotic-fermented milk is commonly used to maintain intestinal health. However, the effects of heat-treated fermented milk, which does not contain live microorganisms, on function are yet fully understood. This study aimed investigate whether Lactobacillus helveticus CP790-fermented affects fecal microbiota and gut health as a “postbiotic”. A randomized, double-blind, placebo-controlled trial was conducted in healthy Japanese individuals aged 20–59 years with tendency toward constipation. Participants consumed 100 mL either test beverage (n = 60) or placebo for four weeks. The beverages were prepared while nonfermented flavored lactic acid. Fecal samples analyzed using 16S rRNA gene sequencing. Constipation symptoms assessed defecation logs Patient Assessment Symptoms (PAC-SYM) questionnaire. Mood state also Profile States 2 (POMS2) questionnaire explore its potential “psychobiotic”. Desulfobacterota significantly decreased by intake. PICRUSt2 analysis predicted decrease proportion genes involved sulfate reduction pathway following consumption milk. intervention improved stool consistency straining during defecation. These improvements correlated Desulfobacterota. After intervention, overall mood, expressed total mood disturbance, depression–dejection better CP790 group than group. results suggest that intake could be effective modulating improving constipation states.

Language: Английский

Citations

2
The effects of Mineral Waters from Slănic Moldova's Spring 1 and Spring 1 bis on Fibroblast activity: An In Vitro Study DOI Creative Commons
Constantin Munteanu,

Mihail Hoteteu,

Diana Munteanu

et al.

Balneo and PRM Research Journal, Journal Year: 2023, Volume and Issue: Vol.14, no.3, P. 591 - 591

Published: Sept. 1, 2023

Primary fibroblast cultures obtained from Wistar rats were investigated with a focus on two vital physiological mechanisms: inflammatory processes and oxidative stress balance. These are believed to be affected by mud sulfurous natural mineral waters, forming the fundamental biological basis for understanding therapeutic effects of these substances. Existing scientific research highlights that various cell types, including fibroblasts, recruited during inflammation. cells respond wide array intercellular microenvironmental signals, leading regulated production both pro- anti-inflammatory mediators. Examples include cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, well chemokines enzymes like cyclooxygenase (COX)-2. Together, play roles in modulating response.

Language: Английский

Citations

5
H2S-sensing properties of flame-synthesized RuO2-decorated WO3 nanoparticulate spin-coated films DOI

Mameaseng Siriwalai,

Matawee Punginsang,

Kanittha Inyawilert

et al.

Materials Science and Engineering B, Journal Year: 2023, Volume and Issue: 299, P. 116968 - 116968

Published: Oct. 27, 2023

Language: Английский

Citations

4
Demonstrating the Beneficial Effect of Low Protein Diet in Primary Sclerosing Cholangitis through a Randomized Clinical Trial and Multi-omics Data Analysis DOI Open Access

Xiaole Yin,

Gila Sasson,

Zheng Sun

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 27, 2024

ABSTRACT Primary sclerosing cholangitis (PSC), a progressive cholestatic hepatobiliary disease characterized by inflammation and fibrosis of the bile ducts, has pathophysiology that is not understood. No effective therapies exist. The only treatment option for PSC liver transplant. We undertook pilot randomized trial diet to investigate disease, role advance potential therapy. enrolled 20 patients with randomly assigned them Low Protein/low sulfur Diet (LPD, n=10) or Specific Carbohydrate (SCD, 8 weeks. Results showed low protein intake benefits patients, whereas higher levels exacerbate condition. further identified gut bacterial markers useful distinguishing LPD responders (mostly concomitant ulcerative colitis) from non-responders. Additionally, integrating multi-omics data, we propose this modifies intestinal cycle reducing hydrogen sulfide (H 2 S) production. Our findings provide an understanding beneficial effect as well insights into possible key driver in PSC.

Language: Английский

Citations

1
Effects of rearing systems (cage versus floor) on the microbial composition and transcriptome of goose ileum DOI Creative Commons

Zhiyu He,

Xuejian Li, Xi Zhang

et al.

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: May 23, 2024

There is a gradual transition from water to dryland rearing of geese. In this study, we performed 16S rRNA sequencing (16S rRNA-seq) and transcriptome (RNA-seq) reveal the effects cage (CR) floor (FR) systems on microbial composition goose ileum. Through rRNA-seq, Linear Discriminant Analysis Effect Size (LEfSe) analysis identified 2 ( hgcI_clade Faecalibacterium ) 14 Bacteroides , Proteiniphilum Proteiniclasticum etc.) differential microbiota in CR FR, respectively. The system influenced 4 pathways including biosynthesis amino acids ileal microbiota. Moreover, 1,198 differentially expressed genes (DEGs) ileum mucosa, with 957 up-regulated 241 FR. CR, Kyoto Encyclopedia Genes Genomes (KEGG) pathway revealed significant enrichment p < 0.05) 28 KEGG pathways, most which were associated acid metabolism. DEGs mainly enriched cellular processes, apoptosis, necroptosis, senescence. Spearman correlation metabolism-related showed positive correlation. Additionally, Phascolarctobacterium Sutterella positively correlated FGF10 PIK3R1 0.01), conclusion, there might be differences metabolism levels between FR geese, observed increase harmful bacterial species impact activity cells.

Language: Английский

Citations

1